---
_id: '1558'
abstract:
- lang: eng
  text: CyclophilinAis a conserved peptidyl-prolyl cis-trans isomerase (PPIase) best
    known as the cellular receptor of the immunosuppressant cyclosporine A. Despite
    significant effort, evidence of developmental functions of cyclophilin A in non-plant
    systems has remained obscure. Mutations in a tomato (Solanum lycopersicum) cyclophilin
    A ortholog, DIAGEOTROPICA (DGT), have been shown to abolish the organogenesis
    of lateral roots; however, a mechanistic explanation of the phenotype is lacking.
    Here, we show that the dgt mutant lacks auxin maxima relevant to priming and specification
    of lateral root founder cells. DGT is expressed in shoot and root, and localizes
    to both the nucleus and cytoplasm during lateral root organogenesis. Mutation
    of ENTIRE/ IAA9, a member of the auxin-responsive Aux/IAA protein family of transcriptional
    repressors, partially restores the inability of dgt to initiate lateral root primordia
    but not the primordia outgrowth. By comparison, grafting of a wild-type scion
    restores the process of lateral root formation, consistent with participation
    of a mobile signal. Antibodies do not detect movement of the DGT protein into
    the dgt rootstock; however, experiments with radiolabeled auxin and an auxin-specific
    microelectrode demonstrate abnormal auxin fluxes. Functional studies of DGT in
    heterologous yeast and tobacco-leaf auxin-transport systems demonstrate that DGT
    negatively regulates PIN-FORMED (PIN) auxin efflux transporters by affecting their
    plasma membrane localization. Studies in tomato support complex effects of the
    dgt mutation on PIN expression level, expression domain and plasma membrane localization.
    Our data demonstrate that DGT regulates auxin transport in lateral root formation.
author:
- first_name: Maria
  full_name: Ivanchenko, Maria
  last_name: Ivanchenko
- first_name: Jinsheng
  full_name: Zhu, Jinsheng
  last_name: Zhu
- first_name: Bangjun
  full_name: Wang, Bangjun
  last_name: Wang
- first_name: Eva
  full_name: Medvecka, Eva
  id: 298814E2-F248-11E8-B48F-1D18A9856A87
  last_name: Medvecka
- first_name: Yunlong
  full_name: Du, Yunlong
  last_name: Du
- first_name: Elisa
  full_name: Azzarello, Elisa
  last_name: Azzarello
- first_name: Stefano
  full_name: Mancuso, Stefano
  last_name: Mancuso
- first_name: Molly
  full_name: Megraw, Molly
  last_name: Megraw
- first_name: Sergei
  full_name: Filichkin, Sergei
  last_name: Filichkin
- first_name: Joseph
  full_name: Dubrovsky, Joseph
  last_name: Dubrovsky
- first_name: Jirí
  full_name: Friml, Jirí
  id: 4159519E-F248-11E8-B48F-1D18A9856A87
  last_name: Friml
  orcid: 0000-0002-8302-7596
- first_name: Markus
  full_name: Geisler, Markus
  last_name: Geisler
citation:
  ama: Ivanchenko M, Zhu J, Wang B, et al. The cyclophilin a DIAGEOTROPICA gene affects
    auxin transport in both root and shoot to control lateral root formation. <i>Development</i>.
    2015;142(4):712-721. doi:<a href="https://doi.org/10.1242/dev.113225">10.1242/dev.113225</a>
  apa: Ivanchenko, M., Zhu, J., Wang, B., Medvecka, E., Du, Y., Azzarello, E., … Geisler,
    M. (2015). The cyclophilin a DIAGEOTROPICA gene affects auxin transport in both
    root and shoot to control lateral root formation. <i>Development</i>. Company
    of Biologists. <a href="https://doi.org/10.1242/dev.113225">https://doi.org/10.1242/dev.113225</a>
  chicago: Ivanchenko, Maria, Jinsheng Zhu, Bangjun Wang, Eva Medvecka, Yunlong Du,
    Elisa Azzarello, Stefano Mancuso, et al. “The Cyclophilin a DIAGEOTROPICA Gene
    Affects Auxin Transport in Both Root and Shoot to Control Lateral Root Formation.”
    <i>Development</i>. Company of Biologists, 2015. <a href="https://doi.org/10.1242/dev.113225">https://doi.org/10.1242/dev.113225</a>.
  ieee: M. Ivanchenko <i>et al.</i>, “The cyclophilin a DIAGEOTROPICA gene affects
    auxin transport in both root and shoot to control lateral root formation,” <i>Development</i>,
    vol. 142, no. 4. Company of Biologists, pp. 712–721, 2015.
  ista: Ivanchenko M, Zhu J, Wang B, Medvecka E, Du Y, Azzarello E, Mancuso S, Megraw
    M, Filichkin S, Dubrovsky J, Friml J, Geisler M. 2015. The cyclophilin a DIAGEOTROPICA
    gene affects auxin transport in both root and shoot to control lateral root formation.
    Development. 142(4), 712–721.
  mla: Ivanchenko, Maria, et al. “The Cyclophilin a DIAGEOTROPICA Gene Affects Auxin
    Transport in Both Root and Shoot to Control Lateral Root Formation.” <i>Development</i>,
    vol. 142, no. 4, Company of Biologists, 2015, pp. 712–21, doi:<a href="https://doi.org/10.1242/dev.113225">10.1242/dev.113225</a>.
  short: M. Ivanchenko, J. Zhu, B. Wang, E. Medvecka, Y. Du, E. Azzarello, S. Mancuso,
    M. Megraw, S. Filichkin, J. Dubrovsky, J. Friml, M. Geisler, Development 142 (2015)
    712–721.
date_created: 2018-12-11T11:52:42Z
date_published: 2015-02-15T00:00:00Z
date_updated: 2021-01-12T06:51:35Z
day: '15'
department:
- _id: JiFr
doi: 10.1242/dev.113225
intvolume: '       142'
issue: '4'
language:
- iso: eng
month: '02'
oa_version: None
page: 712 - 721
publication: Development
publication_status: published
publisher: Company of Biologists
publist_id: '5613'
quality_controlled: '1'
scopus_import: 1
status: public
title: The cyclophilin a DIAGEOTROPICA gene affects auxin transport in both root and
  shoot to control lateral root formation
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 142
year: '2015'
...