---
_id: '14251'
abstract:
- lang: eng
  text: The phytohormone auxin and its directional transport through tissues play
    a fundamental role in development of higher plants. This polar auxin transport
    predominantly relies on PIN-FORMED (PIN) auxin exporters. Hence, PIN polarization
    is crucial for development, but its evolution during the rise of morphological
    complexity in land plants remains unclear. Here, we performed a cross-species
    investigation by observing the trafficking and localization of endogenous and
    exogenous PINs in two bryophytes, Physcomitrium patens and Marchantia polymorpha,
    and in the flowering plant Arabidopsis thaliana. We confirmed that the GFP fusion
    did not compromise the auxin export function of all examined PINs by using radioactive
    auxin export assay and by observing the phenotypic changes in transgenic bryophytes.
    Endogenous PINs polarize to filamentous apices, while exogenous Arabidopsis PINs
    distribute symmetrically on the membrane in both bryophytes. In Arabidopsis root
    epidermis, bryophytic PINs show no defined polarity. Pharmacological interference
    revealed a strong cytoskeleton dependence of bryophytic but not Arabidopsis PIN
    polarization. The divergence of PIN polarization and trafficking is also observed
    within the bryophyte clade and between tissues of individual species. These results
    collectively reveal a divergence of PIN trafficking and polarity mechanisms throughout
    land plant evolution and a co-evolution of PIN sequence-based and cell-based polarity
    mechanisms.
acknowledgement: This work was supported by the ERC grant (PR1023ERC02) to H. T. and
  J. F., and by the ministry of science and technology (grant number 110-2636-B-005-001)
  to K. J. L.
article_number: '100669'
article_processing_charge: Yes
article_type: original
author:
- first_name: Han
  full_name: Tang, Han
  id: 19BDF720-25A0-11EA-AC6E-928F3DDC885E
  last_name: Tang
  orcid: 0000-0001-6152-6637
- first_name: KJ
  full_name: Lu, KJ
  last_name: Lu
- first_name: Y
  full_name: Zhang, Y
  last_name: Zhang
- first_name: YL
  full_name: Cheng, YL
  last_name: Cheng
- first_name: SL
  full_name: Tu, SL
  last_name: Tu
- first_name: Jiří
  full_name: Friml, Jiří
  id: 4159519E-F248-11E8-B48F-1D18A9856A87
  last_name: Friml
  orcid: 0000-0002-8302-7596
citation:
  ama: Tang H, Lu K, Zhang Y, Cheng Y, Tu S, Friml J. Divergence of trafficking and
    polarization mechanisms for PIN auxin transporters during land plant evolution.
    <i>Plant Communications</i>. 2024;5(1). doi:<a href="https://doi.org/10.1016/j.xplc.2023.100669">10.1016/j.xplc.2023.100669</a>
  apa: Tang, H., Lu, K., Zhang, Y., Cheng, Y., Tu, S., &#38; Friml, J. (2024). Divergence
    of trafficking and polarization mechanisms for PIN auxin transporters during land
    plant evolution. <i>Plant Communications</i>. Elsevier. <a href="https://doi.org/10.1016/j.xplc.2023.100669">https://doi.org/10.1016/j.xplc.2023.100669</a>
  chicago: Tang, Han, KJ Lu, Y Zhang, YL Cheng, SL Tu, and Jiří Friml. “Divergence
    of Trafficking and Polarization Mechanisms for PIN Auxin Transporters during Land
    Plant Evolution.” <i>Plant Communications</i>. Elsevier, 2024. <a href="https://doi.org/10.1016/j.xplc.2023.100669">https://doi.org/10.1016/j.xplc.2023.100669</a>.
  ieee: H. Tang, K. Lu, Y. Zhang, Y. Cheng, S. Tu, and J. Friml, “Divergence of trafficking
    and polarization mechanisms for PIN auxin transporters during land plant evolution,”
    <i>Plant Communications</i>, vol. 5, no. 1. Elsevier, 2024.
  ista: Tang H, Lu K, Zhang Y, Cheng Y, Tu S, Friml J. 2024. Divergence of trafficking
    and polarization mechanisms for PIN auxin transporters during land plant evolution.
    Plant Communications. 5(1), 100669.
  mla: Tang, Han, et al. “Divergence of Trafficking and Polarization Mechanisms for
    PIN Auxin Transporters during Land Plant Evolution.” <i>Plant Communications</i>,
    vol. 5, no. 1, 100669, Elsevier, 2024, doi:<a href="https://doi.org/10.1016/j.xplc.2023.100669">10.1016/j.xplc.2023.100669</a>.
  short: H. Tang, K. Lu, Y. Zhang, Y. Cheng, S. Tu, J. Friml, Plant Communications
    5 (2024).
date_created: 2023-09-01T11:32:02Z
date_published: 2024-01-08T00:00:00Z
date_updated: 2025-07-02T12:51:02Z
day: '08'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1016/j.xplc.2023.100669
ec_funded: 1
external_id:
  pmid:
  - '37528584'
file:
- access_level: open_access
  checksum: edbc44c6d4a394d2bf70f92fdbb08f0a
  content_type: application/pdf
  creator: dernst
  date_created: 2024-01-30T12:59:57Z
  date_updated: 2024-01-30T12:59:57Z
  file_id: '14911'
  file_name: 2023_PlantCommunications_Tang.pdf
  file_size: 2825565
  relation: main_file
  success: 1
file_date_updated: 2024-01-30T12:59:57Z
has_accepted_license: '1'
intvolume: '         5'
issue: '1'
language:
- iso: eng
month: '01'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
  call_identifier: H2020
  grant_number: '742985'
  name: Tracing Evolution of Auxin Transport and Polarity in Plants
publication: Plant Communications
publication_identifier:
  issn:
  - 2590-3462
  issnl:
  - 1234-4567
publication_status: published
publisher: Elsevier
quality_controlled: '1'
scopus_import: '1'
status: public
title: Divergence of trafficking and polarization mechanisms for PIN auxin transporters
  during land plant evolution
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 5
year: '2024'
...