---
_id: '14447'
abstract:
- lang: eng
  text: "Auxin belongs among major phytohormones and governs multiple aspects of plant
    growth and development. The establishment of auxin concentration gradients, determines,
    among other processes, plant organ positioning and growth responses to environmental
    stimuli.\r\nHerein we report the synthesis of new NBD- or DNS-labelled IAA derivatives
    and the elucidation of their biological activity, fluorescence properties and
    subcellular accumulation patterns in planta. These novel compounds did not show
    auxin-like activity, but instead antagonized physiological auxin effects. The
    DNS-labelled derivatives FL5 and FL6 showed strong anti-auxin activity in roots
    and hypocotyls, which also occurred at the level of gene transcription as confirmed
    by quantitative PCR analysis. The auxin antagonism of our derivatives was further
    demonstrated in vitro using an SPR-based binding assay. The NBD-labelled compound
    FL4 with the best fluorescence properties proved to be unsuitable to study auxin
    accumulation patterns in planta. On the other hand, the strongest anti-auxin activity
    possessing compounds FL5 and FL6 could be useful to study binding mechanisms to
    auxin receptors and for manipulations of auxin-regulated processes."
acknowledgement: The authors would like to thank Karolína Kubiasová and Iñigo Saiz-Fernández
  for valuable scientific discussions. Open access publishing supported by the National
  Technical Library in Prague. This work was supported by the Palacký University Olomouc
  Young Researcher Grant Competition (JG_2020_002), by the Internal Grant Agency of
  Palacký University Olomouc (IGA_PrF_2023_016, IGA_PrF_2023_031), by the Ministry
  of Education, Youth and Sports of the Czech Republic through the European Regional
  Development Fund-Project Plants as a tool for sustainable global development (CZ.02.1.01/0.0/0.0/16_019/0000827)
  and the project Support of mobility at Palacký University Olomouc II. (CZ.02.2.69/0.0/0.0/18_053/0016919).
  The Biacore T200 SPR instrument was provided by the WISB Research Technology Facility
  within the School of Life Sciences, University of Warwick.
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Kristýna
  full_name: Bieleszová, Kristýna
  last_name: Bieleszová
- first_name: Pavel
  full_name: Hladík, Pavel
  last_name: Hladík
- first_name: Martin
  full_name: Kubala, Martin
  last_name: Kubala
- first_name: Richard
  full_name: Napier, Richard
  last_name: Napier
- first_name: Federica
  full_name: Brunoni, Federica
  last_name: Brunoni
- first_name: Zuzana
  full_name: Gelová, Zuzana
  id: 0AE74790-0E0B-11E9-ABC7-1ACFE5697425
  last_name: Gelová
  orcid: 0000-0003-4783-1752
- first_name: Lukas
  full_name: Fiedler, Lukas
  id: 7c417475-8972-11ed-ae7b-8b674ca26986
  last_name: Fiedler
- first_name: Ivan
  full_name: Kulich, Ivan
  id: 57a1567c-8314-11eb-9063-c9ddc3451a54
  last_name: Kulich
- first_name: Miroslav
  full_name: Strnad, Miroslav
  last_name: Strnad
- first_name: Karel
  full_name: Doležal, Karel
  last_name: Doležal
- first_name: Ondřej
  full_name: Novák, Ondřej
  last_name: Novák
- first_name: Jiří
  full_name: Friml, Jiří
  id: 4159519E-F248-11E8-B48F-1D18A9856A87
  last_name: Friml
  orcid: 0000-0002-8302-7596
- first_name: Asta
  full_name: Žukauskaitė, Asta
  last_name: Žukauskaitė
citation:
  ama: 'Bieleszová K, Hladík P, Kubala M, et al. New fluorescent auxin derivatives:
    anti-auxin activity and accumulation patterns in Arabidopsis thaliana. <i>Plant
    Growth Regulation</i>. 2023. doi:<a href="https://doi.org/10.1007/s10725-023-01083-0">10.1007/s10725-023-01083-0</a>'
  apa: 'Bieleszová, K., Hladík, P., Kubala, M., Napier, R., Brunoni, F., Gelová, Z.,
    … Žukauskaitė, A. (2023). New fluorescent auxin derivatives: anti-auxin activity
    and accumulation patterns in Arabidopsis thaliana. <i>Plant Growth Regulation</i>.
    Springer Nature. <a href="https://doi.org/10.1007/s10725-023-01083-0">https://doi.org/10.1007/s10725-023-01083-0</a>'
  chicago: 'Bieleszová, Kristýna, Pavel Hladík, Martin Kubala, Richard Napier, Federica
    Brunoni, Zuzana Gelová, Lukas Fiedler, et al. “New Fluorescent Auxin Derivatives:
    Anti-Auxin Activity and Accumulation Patterns in Arabidopsis Thaliana.” <i>Plant
    Growth Regulation</i>. Springer Nature, 2023. <a href="https://doi.org/10.1007/s10725-023-01083-0">https://doi.org/10.1007/s10725-023-01083-0</a>.'
  ieee: 'K. Bieleszová <i>et al.</i>, “New fluorescent auxin derivatives: anti-auxin
    activity and accumulation patterns in Arabidopsis thaliana,” <i>Plant Growth Regulation</i>.
    Springer Nature, 2023.'
  ista: 'Bieleszová K, Hladík P, Kubala M, Napier R, Brunoni F, Gelová Z, Fiedler
    L, Kulich I, Strnad M, Doležal K, Novák O, Friml J, Žukauskaitė A. 2023. New fluorescent
    auxin derivatives: anti-auxin activity and accumulation patterns in Arabidopsis
    thaliana. Plant Growth Regulation.'
  mla: 'Bieleszová, Kristýna, et al. “New Fluorescent Auxin Derivatives: Anti-Auxin
    Activity and Accumulation Patterns in Arabidopsis Thaliana.” <i>Plant Growth Regulation</i>,
    Springer Nature, 2023, doi:<a href="https://doi.org/10.1007/s10725-023-01083-0">10.1007/s10725-023-01083-0</a>.'
  short: K. Bieleszová, P. Hladík, M. Kubala, R. Napier, F. Brunoni, Z. Gelová, L.
    Fiedler, I. Kulich, M. Strnad, K. Doležal, O. Novák, J. Friml, A. Žukauskaitė,
    Plant Growth Regulation (2023).
date_created: 2023-10-22T22:01:15Z
date_published: 2023-10-13T00:00:00Z
date_updated: 2023-12-13T13:08:25Z
day: '13'
department:
- _id: JiFr
doi: 10.1007/s10725-023-01083-0
external_id:
  isi:
  - '001084334300001'
isi: 1
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: https://doi.org/10.1007/s10725-023-01083-0
month: '10'
oa: 1
oa_version: Published Version
publication: Plant Growth Regulation
publication_identifier:
  eissn:
  - 1573-5087
  issn:
  - 0167-6903
publication_status: epub_ahead
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
status: public
title: 'New fluorescent auxin derivatives: anti-auxin activity and accumulation patterns
  in Arabidopsis thaliana'
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '2023'
...
---
_id: '12291'
abstract:
- lang: eng
  text: The phytohormone auxin triggers transcriptional reprogramming through a well-characterized
    perception machinery in the nucleus. By contrast, mechanisms that underlie fast
    effects of auxin, such as the regulation of ion fluxes, rapid phosphorylation
    of proteins or auxin feedback on its transport, remain unclear1,2,3. Whether auxin-binding
    protein 1 (ABP1) is an auxin receptor has been a source of debate for decades1,4.
    Here we show that a fraction of Arabidopsis thaliana ABP1 is secreted and binds
    auxin specifically at an acidic pH that is typical of the apoplast. ABP1 and its
    plasma-membrane-localized partner, transmembrane kinase 1 (TMK1), are required
    for the auxin-induced ultrafast global phospho-response and for downstream processes
    that include the activation of H+-ATPase and accelerated cytoplasmic streaming.
    abp1 and tmk mutants cannot establish auxin-transporting channels and show defective
    auxin-induced vasculature formation and regeneration. An ABP1(M2X) variant that
    lacks the capacity to bind auxin is unable to complement these defects in abp1
    mutants. These data indicate that ABP1 is the auxin receptor for TMK1-based cell-surface
    signalling, which mediates the global phospho-response and auxin canalization.
acknowledged_ssus:
- _id: Bio
- _id: EM-Fac
- _id: LifeSc
acknowledgement: We acknowledge K. Kubiasová for excellent technical assistance, J.
  Neuhold, A. Lehner and A. Sedivy for technical assistance with protein production
  and purification at Vienna Biocenter Core Facilities; Creoptix for performing GCI;
  and the Bioimaging, Electron Microscopy and Life Science Facilities at ISTA, the
  Plant Sciences Core Facility of CEITEC Masaryk University, the Core Facility CELLIM
  (MEYS CR, LM2018129 Czech-BioImaging) and J. Sprakel for their assistance. J.F.
  is grateful to R. Napier for many insightful suggestions and support. We thank all
  past and present members of the Friml group for their support and for other contributions
  to this effort to clarify the controversial role of ABP1 over the past seven years.
  The project received funding from the European Research Council (ERC) under the
  European Union’s Horizon 2020 research and innovation program (grant agreement no.
  742985 to J.F. and 833867 to D.W.); the Austrian Science Fund (FWF; P29988 to J.F.);
  the Netherlands Organization for Scientific Research (NWO; VICI grant 865.14.001
  to D.W. and VENI grant VI.Veni.212.003 to A.K.); the Ministry of Education, Science
  and Technological Development of the Republic of Serbia (contract no. 451-03-68/2022-14/200053
  to B.D.Ž.); and the MEXT/JSPS KAKENHI to K.T. (20K06685) and T.K. (20H05687 and
  20H05910).
article_processing_charge: No
article_type: original
author:
- first_name: Jiří
  full_name: Friml, Jiří
  id: 4159519E-F248-11E8-B48F-1D18A9856A87
  last_name: Friml
  orcid: 0000-0002-8302-7596
- first_name: Michelle C
  full_name: Gallei, Michelle C
  id: 35A03822-F248-11E8-B48F-1D18A9856A87
  last_name: Gallei
  orcid: 0000-0003-1286-7368
- first_name: Zuzana
  full_name: Gelová, Zuzana
  id: 0AE74790-0E0B-11E9-ABC7-1ACFE5697425
  last_name: Gelová
  orcid: 0000-0003-4783-1752
- first_name: Alexander J
  full_name: Johnson, Alexander J
  id: 46A62C3A-F248-11E8-B48F-1D18A9856A87
  last_name: Johnson
  orcid: 0000-0002-2739-8843
- first_name: Ewa
  full_name: Mazur, Ewa
  last_name: Mazur
- first_name: Aline
  full_name: Monzer, Aline
  id: 2DB5D88C-D7B3-11E9-B8FD-7907E6697425
  last_name: Monzer
- first_name: Lesia
  full_name: Rodriguez Solovey, Lesia
  id: 3922B506-F248-11E8-B48F-1D18A9856A87
  last_name: Rodriguez Solovey
  orcid: 0000-0002-7244-7237
- first_name: Mark
  full_name: Roosjen, Mark
  last_name: Roosjen
- first_name: Inge
  full_name: Verstraeten, Inge
  id: 362BF7FE-F248-11E8-B48F-1D18A9856A87
  last_name: Verstraeten
  orcid: 0000-0001-7241-2328
- first_name: Branka D.
  full_name: Živanović, Branka D.
  last_name: Živanović
- first_name: Minxia
  full_name: Zou, Minxia
  id: 5c243f41-03f3-11ec-841c-96faf48a7ef9
  last_name: Zou
- first_name: Lukas
  full_name: Fiedler, Lukas
  id: 7c417475-8972-11ed-ae7b-8b674ca26986
  last_name: Fiedler
- first_name: Caterina
  full_name: Giannini, Caterina
  id: e3fdddd5-f6e0-11ea-865d-ca99ee6367f4
  last_name: Giannini
- first_name: Peter
  full_name: Grones, Peter
  last_name: Grones
- first_name: Mónika
  full_name: Hrtyan, Mónika
  id: 45A71A74-F248-11E8-B48F-1D18A9856A87
  last_name: Hrtyan
- first_name: Walter
  full_name: Kaufmann, Walter
  id: 3F99E422-F248-11E8-B48F-1D18A9856A87
  last_name: Kaufmann
  orcid: 0000-0001-9735-5315
- first_name: Andre
  full_name: Kuhn, Andre
  last_name: Kuhn
- first_name: Madhumitha
  full_name: Narasimhan, Madhumitha
  id: 44BF24D0-F248-11E8-B48F-1D18A9856A87
  last_name: Narasimhan
  orcid: 0000-0002-8600-0671
- first_name: Marek
  full_name: Randuch, Marek
  id: 6ac4636d-15b2-11ec-abd3-fb8df79972ae
  last_name: Randuch
- first_name: Nikola
  full_name: Rýdza, Nikola
  last_name: Rýdza
- first_name: Koji
  full_name: Takahashi, Koji
  last_name: Takahashi
- first_name: Shutang
  full_name: Tan, Shutang
  id: 2DE75584-F248-11E8-B48F-1D18A9856A87
  last_name: Tan
  orcid: 0000-0002-0471-8285
- first_name: Anastasiia
  full_name: Teplova, Anastasiia
  id: e3736151-106c-11ec-b916-c2558e2762c6
  last_name: Teplova
- first_name: Toshinori
  full_name: Kinoshita, Toshinori
  last_name: Kinoshita
- first_name: Dolf
  full_name: Weijers, Dolf
  last_name: Weijers
- first_name: Hana
  full_name: Rakusová, Hana
  last_name: Rakusová
citation:
  ama: Friml J, Gallei MC, Gelová Z, et al. ABP1–TMK auxin perception for global phosphorylation
    and auxin canalization. <i>Nature</i>. 2022;609(7927):575-581. doi:<a href="https://doi.org/10.1038/s41586-022-05187-x">10.1038/s41586-022-05187-x</a>
  apa: Friml, J., Gallei, M. C., Gelová, Z., Johnson, A. J., Mazur, E., Monzer, A.,
    … Rakusová, H. (2022). ABP1–TMK auxin perception for global phosphorylation and
    auxin canalization. <i>Nature</i>. Springer Nature. <a href="https://doi.org/10.1038/s41586-022-05187-x">https://doi.org/10.1038/s41586-022-05187-x</a>
  chicago: Friml, Jiří, Michelle C Gallei, Zuzana Gelová, Alexander J Johnson, Ewa
    Mazur, Aline Monzer, Lesia Rodriguez Solovey, et al. “ABP1–TMK Auxin Perception
    for Global Phosphorylation and Auxin Canalization.” <i>Nature</i>. Springer Nature,
    2022. <a href="https://doi.org/10.1038/s41586-022-05187-x">https://doi.org/10.1038/s41586-022-05187-x</a>.
  ieee: J. Friml <i>et al.</i>, “ABP1–TMK auxin perception for global phosphorylation
    and auxin canalization,” <i>Nature</i>, vol. 609, no. 7927. Springer Nature, pp.
    575–581, 2022.
  ista: Friml J, Gallei MC, Gelová Z, Johnson AJ, Mazur E, Monzer A, Rodriguez Solovey
    L, Roosjen M, Verstraeten I, Živanović BD, Zou M, Fiedler L, Giannini C, Grones
    P, Hrtyan M, Kaufmann W, Kuhn A, Narasimhan M, Randuch M, Rýdza N, Takahashi K,
    Tan S, Teplova A, Kinoshita T, Weijers D, Rakusová H. 2022. ABP1–TMK auxin perception
    for global phosphorylation and auxin canalization. Nature. 609(7927), 575–581.
  mla: Friml, Jiří, et al. “ABP1–TMK Auxin Perception for Global Phosphorylation and
    Auxin Canalization.” <i>Nature</i>, vol. 609, no. 7927, Springer Nature, 2022,
    pp. 575–81, doi:<a href="https://doi.org/10.1038/s41586-022-05187-x">10.1038/s41586-022-05187-x</a>.
  short: J. Friml, M.C. Gallei, Z. Gelová, A.J. Johnson, E. Mazur, A. Monzer, L. Rodriguez
    Solovey, M. Roosjen, I. Verstraeten, B.D. Živanović, M. Zou, L. Fiedler, C. Giannini,
    P. Grones, M. Hrtyan, W. Kaufmann, A. Kuhn, M. Narasimhan, M. Randuch, N. Rýdza,
    K. Takahashi, S. Tan, A. Teplova, T. Kinoshita, D. Weijers, H. Rakusová, Nature
    609 (2022) 575–581.
date_created: 2023-01-16T10:04:48Z
date_published: 2022-09-15T00:00:00Z
date_updated: 2023-11-07T08:16:09Z
day: '15'
ddc:
- '580'
department:
- _id: JiFr
- _id: GradSch
- _id: EvBe
- _id: EM-Fac
doi: 10.1038/s41586-022-05187-x
ec_funded: 1
external_id:
  isi:
  - '000851357500002'
  pmid:
  - '36071161'
file:
- access_level: open_access
  checksum: a6055c606aefb900bf62ae3e7d15f921
  content_type: application/pdf
  creator: amally
  date_created: 2023-11-02T17:12:37Z
  date_updated: 2023-11-02T17:12:37Z
  file_id: '14483'
  file_name: Friml Nature 2022_merged.pdf
  file_size: 79774945
  relation: main_file
  success: 1
file_date_updated: 2023-11-02T17:12:37Z
has_accepted_license: '1'
intvolume: '       609'
isi: 1
issue: '7927'
language:
- iso: eng
month: '09'
oa: 1
oa_version: Submitted Version
page: 575-581
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
  call_identifier: H2020
  grant_number: '742985'
  name: Tracing Evolution of Auxin Transport and Polarity in Plants
- _id: 262EF96E-B435-11E9-9278-68D0E5697425
  call_identifier: FWF
  grant_number: P29988
  name: RNA-directed DNA methylation in plant development
publication: Nature
publication_identifier:
  eissn:
  - 1476-4687
  issn:
  - 0028-0836
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
status: public
title: ABP1–TMK auxin perception for global phosphorylation and auxin canalization
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 609
year: '2022'
...
---
_id: '8931'
abstract:
- lang: eng
  text: "Auxin is a major plant growth regulator, but current models on auxin perception
    and signaling cannot explain the whole plethora of auxin effects, in particular
    those associated with rapid responses. A possible candidate for a component of
    additional auxin perception mechanisms is the AUXIN BINDING PROTEIN 1 (ABP1),
    whose function in planta remains unclear.\r\nHere we combined expression analysis
    with gain- and loss-of-function approaches to analyze the role of ABP1 in plant
    development. ABP1 shows a broad expression largely overlapping with, but not regulated
    by, transcriptional auxin response activity. Furthermore, ABP1 activity is not
    essential for the transcriptional auxin signaling. Genetic in planta analysis
    revealed that abp1 loss-of-function mutants show largely normal development with
    minor defects in bolting. On the other hand, ABP1 gain-of-function alleles show
    a broad range of growth and developmental defects, including root and hypocotyl
    growth and bending, lateral root and leaf development, bolting, as well as response
    to heat stress. At the cellular level, ABP1 gain-of-function leads to impaired
    auxin effect on PIN polar distribution and affects BFA-sensitive PIN intracellular
    aggregation.\r\nThe gain-of-function analysis suggests a broad, but still mechanistically
    unclear involvement of ABP1 in plant development, possibly masked in abp1 loss-of-function
    mutants by a functional redundancy."
acknowledged_ssus:
- _id: Bio
- _id: LifeSc
acknowledgement: We would like to acknowledge Bioimaging and Life Science Facilities
  at IST Austria for continuous support and also the Plant Sciences Core Facility
  of CEITEC Masaryk University for their support with obtaining a part of the scientific
  data. We gratefully acknowledge Lindy Abas for help with ABP1::GFP-ABP1 construct
  design. This project has received funding from the European Research Council (ERC)
  under the European Union’s Horizon 2020 research and innovation program [grant agreement
  no. 742985] and Austrian Science Fund (FWF) [I 3630-B25] to J.F.; DOC Fellowship
  of the Austrian Academy of Sciences to L.L.; the European Structural and Investment
  Funds, Operational Programme Research, Development and Education - Project „MSCAfellow@MUNI“
  [CZ.02.2.69/0.0/0.0/17_050/0008496] to M.P.. This project was also supported by
  the Czech Science Foundation [GA 20-20860Y] to M.Z and MEYS CR [project no.CZ.02.1.01/0.0/0.0/16_019/0000738]
  to M. Č.
article_number: '110750'
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Zuzana
  full_name: Gelová, Zuzana
  id: 0AE74790-0E0B-11E9-ABC7-1ACFE5697425
  last_name: Gelová
  orcid: 0000-0003-4783-1752
- first_name: Michelle C
  full_name: Gallei, Michelle C
  id: 35A03822-F248-11E8-B48F-1D18A9856A87
  last_name: Gallei
  orcid: 0000-0003-1286-7368
- first_name: Markéta
  full_name: Pernisová, Markéta
  last_name: Pernisová
- first_name: Géraldine
  full_name: Brunoud, Géraldine
  last_name: Brunoud
- first_name: Xixi
  full_name: Zhang, Xixi
  id: 61A66458-47E9-11EA-85BA-8AEAAF14E49A
  last_name: Zhang
  orcid: 0000-0001-7048-4627
- first_name: Matous
  full_name: Glanc, Matous
  id: 1AE1EA24-02D0-11E9-9BAA-DAF4881429F2
  last_name: Glanc
  orcid: 0000-0003-0619-7783
- first_name: Lanxin
  full_name: Li, Lanxin
  id: 367EF8FA-F248-11E8-B48F-1D18A9856A87
  last_name: Li
  orcid: 0000-0002-5607-272X
- first_name: Jaroslav
  full_name: Michalko, Jaroslav
  id: 483727CA-F248-11E8-B48F-1D18A9856A87
  last_name: Michalko
- first_name: Zlata
  full_name: Pavlovicova, Zlata
  last_name: Pavlovicova
- first_name: Inge
  full_name: Verstraeten, Inge
  id: 362BF7FE-F248-11E8-B48F-1D18A9856A87
  last_name: Verstraeten
  orcid: 0000-0001-7241-2328
- first_name: Huibin
  full_name: Han, Huibin
  id: 31435098-F248-11E8-B48F-1D18A9856A87
  last_name: Han
- first_name: Jakub
  full_name: Hajny, Jakub
  id: 4800CC20-F248-11E8-B48F-1D18A9856A87
  last_name: Hajny
  orcid: 0000-0003-2140-7195
- first_name: Robert
  full_name: Hauschild, Robert
  id: 4E01D6B4-F248-11E8-B48F-1D18A9856A87
  last_name: Hauschild
  orcid: 0000-0001-9843-3522
- first_name: Milada
  full_name: Čovanová, Milada
  last_name: Čovanová
- first_name: Marta
  full_name: Zwiewka, Marta
  last_name: Zwiewka
- first_name: Lukas
  full_name: Hörmayer, Lukas
  id: 2EEE7A2A-F248-11E8-B48F-1D18A9856A87
  last_name: Hörmayer
  orcid: 0000-0001-8295-2926
- first_name: Matyas
  full_name: Fendrych, Matyas
  id: 43905548-F248-11E8-B48F-1D18A9856A87
  last_name: Fendrych
  orcid: 0000-0002-9767-8699
- first_name: Tongda
  full_name: Xu, Tongda
  last_name: Xu
- first_name: Teva
  full_name: Vernoux, Teva
  last_name: Vernoux
- first_name: Jiří
  full_name: Friml, Jiří
  id: 4159519E-F248-11E8-B48F-1D18A9856A87
  last_name: Friml
  orcid: 0000-0002-8302-7596
citation:
  ama: Gelová Z, Gallei MC, Pernisová M, et al. Developmental roles of auxin binding
    protein 1 in Arabidopsis thaliana. <i>Plant Science</i>. 2021;303. doi:<a href="https://doi.org/10.1016/j.plantsci.2020.110750">10.1016/j.plantsci.2020.110750</a>
  apa: Gelová, Z., Gallei, M. C., Pernisová, M., Brunoud, G., Zhang, X., Glanc, M.,
    … Friml, J. (2021). Developmental roles of auxin binding protein 1 in Arabidopsis
    thaliana. <i>Plant Science</i>. Elsevier. <a href="https://doi.org/10.1016/j.plantsci.2020.110750">https://doi.org/10.1016/j.plantsci.2020.110750</a>
  chicago: Gelová, Zuzana, Michelle C Gallei, Markéta Pernisová, Géraldine Brunoud,
    Xixi Zhang, Matous Glanc, Lanxin Li, et al. “Developmental Roles of Auxin Binding
    Protein 1 in Arabidopsis Thaliana.” <i>Plant Science</i>. Elsevier, 2021. <a href="https://doi.org/10.1016/j.plantsci.2020.110750">https://doi.org/10.1016/j.plantsci.2020.110750</a>.
  ieee: Z. Gelová <i>et al.</i>, “Developmental roles of auxin binding protein 1 in
    Arabidopsis thaliana,” <i>Plant Science</i>, vol. 303. Elsevier, 2021.
  ista: Gelová Z, Gallei MC, Pernisová M, Brunoud G, Zhang X, Glanc M, Li L, Michalko
    J, Pavlovicova Z, Verstraeten I, Han H, Hajny J, Hauschild R, Čovanová M, Zwiewka
    M, Hörmayer L, Fendrych M, Xu T, Vernoux T, Friml J. 2021. Developmental roles
    of auxin binding protein 1 in Arabidopsis thaliana. Plant Science. 303, 110750.
  mla: Gelová, Zuzana, et al. “Developmental Roles of Auxin Binding Protein 1 in Arabidopsis
    Thaliana.” <i>Plant Science</i>, vol. 303, 110750, Elsevier, 2021, doi:<a href="https://doi.org/10.1016/j.plantsci.2020.110750">10.1016/j.plantsci.2020.110750</a>.
  short: Z. Gelová, M.C. Gallei, M. Pernisová, G. Brunoud, X. Zhang, M. Glanc, L.
    Li, J. Michalko, Z. Pavlovicova, I. Verstraeten, H. Han, J. Hajny, R. Hauschild,
    M. Čovanová, M. Zwiewka, L. Hörmayer, M. Fendrych, T. Xu, T. Vernoux, J. Friml,
    Plant Science 303 (2021).
date_created: 2020-12-09T14:48:28Z
date_published: 2021-02-01T00:00:00Z
date_updated: 2024-10-29T10:22:43Z
day: '01'
ddc:
- '580'
department:
- _id: JiFr
- _id: Bio
doi: 10.1016/j.plantsci.2020.110750
ec_funded: 1
external_id:
  isi:
  - '000614154500001'
  pmid:
  - '33487339'
file:
- access_level: open_access
  checksum: a7f2562bdca62d67dfa88e271b62a629
  content_type: application/pdf
  creator: dernst
  date_created: 2021-02-04T07:49:25Z
  date_updated: 2021-02-04T07:49:25Z
  file_id: '9083'
  file_name: 2021_PlantScience_Gelova.pdf
  file_size: 12563728
  relation: main_file
  success: 1
file_date_updated: 2021-02-04T07:49:25Z
has_accepted_license: '1'
intvolume: '       303'
isi: 1
keyword:
- Agronomy and Crop Science
- Plant Science
- Genetics
- General Medicine
language:
- iso: eng
month: '02'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
  call_identifier: H2020
  grant_number: '742985'
  name: Tracing Evolution of Auxin Transport and Polarity in Plants
- _id: 26538374-B435-11E9-9278-68D0E5697425
  call_identifier: FWF
  grant_number: I03630
  name: Molecular mechanisms of endocytic cargo recognition in plants
- _id: 26B4D67E-B435-11E9-9278-68D0E5697425
  grant_number: '25351'
  name: 'A Case Study of Plant Growth Regulation: Molecular Mechanism of Auxin-mediated
    Rapid Growth Inhibition in Arabidopsis Root'
publication: Plant Science
publication_identifier:
  issn:
  - 0168-9452
publication_status: published
publisher: Elsevier
quality_controlled: '1'
related_material:
  record:
  - id: '11626'
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    status: public
  - id: '10083'
    relation: dissertation_contains
    status: public
scopus_import: '1'
status: public
title: Developmental roles of auxin binding protein 1 in Arabidopsis thaliana
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 303
year: '2021'
...
---
_id: '8337'
abstract:
- lang: eng
  text: Cytokinins are mobile multifunctional plant hormones with roles in development
    and stress resilience. Although their Histidine Kinase receptors are substantially
    localised to the endoplasmic reticulum, cellular sites of cytokinin perception
    and importance of spatially heterogeneous cytokinin distribution continue to be
    debated. Here we show that cytokinin perception by plasma membrane receptors is
    an effective additional path for cytokinin response. Readout from a Two Component
    Signalling cytokinin-specific reporter (TCSn::GFP) closely matches intracellular
    cytokinin content in roots, yet we also find cytokinins in extracellular fluid,
    potentially enabling action at the cell surface. Cytokinins covalently linked
    to beads that could not pass the plasma membrane increased expression of both
    TCSn::GFP and Cytokinin Response Factors. Super-resolution microscopy of GFP-labelled
    receptors and diminished TCSn::GFP response to immobilised cytokinins in cytokinin
    receptor mutants, further indicate that receptors can function at the cell surface.
    We argue that dual intracellular and surface locations may augment flexibility
    of cytokinin responses.
acknowledged_ssus:
- _id: Bio
acknowledgement: 'We thank Bruno Müller and Aaron Rashotte for critical discussions
  and provision of plant lines used in this work, Roger Granbom and Tamara Hernández
  Verdeja (UPSC, Umeå, Sweden) for technical assistance and providing materials, Zuzana
  Pěkná and Karolina Wojewodová (CRH, Palacký University, Olomouc, Czech Republic)
  for help with cytokinin receptor binding assays, and David Zalabák (CRH, Palacký
  University, Olomouc, Czech Republic) for provision of vector pINIIIΔEH expressing
  CRE1/AHK4. The bioimaging facility of IST Austria, the Swedish Metabolomics Centre
  and the IST Austria Bio-Imaging facility are acknowledged for support. The work
  was funded by the European Molecular Biology Organization (EMBO ASTF 297-2013) (I.A.),
  Development—The Company of Biologists (DEVTF2012) (I.A.; C.T.), Plant Fellows (the
  International Post doc Fellowship Programme in Plant Sciences, 267423) (I.A.; K.L.),
  the Swedish Research Council (621-2014-4514) (K.L.), UPSC Berzelii Center for Forest
  Biotechnology (Vinnova 2012-01560), Kempestiftelserna (JCK-2711) (K.L.) and (JCK-1811)
  (E.-M.B., K.L.). The Ministry of Education, Youth and Sports of the Czech Republic
  via the European Regional Development Fund-Project “Plants as a tool for sustainable
  global development” (CZ.02.1.01/0.0/0.0/16_019/0000827) (O.N., O.P., R.S., V.M.,
  L.P., K.D.) and project CEITEC 2020 (LQ1601) (M.P., J.H.) provided support, as did
  the Czech Science Foundation via projects GP14-30004P (M.P.) and 16-04184S (O.P.,
  K.D., O.N.), Vetenskapsrådet and Vinnova (Verket för Innovationssystem) (T.V., S.R.),
  Knut och Alice Wallenbergs Stiftelse via “Shapesystem” grant number 2012.0050. A.J.
  was supported by the Austria Science Fund (FWF): I03630 to J.F. The research leading
  to these results received funding from European Union’s Horizon 2020 programme (ERC
  grant no. 742985) and FWO-FWF joint project G0E5718N to J.F.'
article_number: '4284'
article_processing_charge: No
article_type: original
author:
- first_name: Ioanna
  full_name: Antoniadi, Ioanna
  last_name: Antoniadi
- first_name: Ondřej
  full_name: Novák, Ondřej
  last_name: Novák
- first_name: Zuzana
  full_name: Gelová, Zuzana
  id: 0AE74790-0E0B-11E9-ABC7-1ACFE5697425
  last_name: Gelová
  orcid: 0000-0003-4783-1752
- first_name: Alexander J
  full_name: Johnson, Alexander J
  id: 46A62C3A-F248-11E8-B48F-1D18A9856A87
  last_name: Johnson
  orcid: 0000-0002-2739-8843
- first_name: Ondřej
  full_name: Plíhal, Ondřej
  last_name: Plíhal
- first_name: Radim
  full_name: Simerský, Radim
  last_name: Simerský
- first_name: Václav
  full_name: Mik, Václav
  last_name: Mik
- first_name: Thomas
  full_name: Vain, Thomas
  last_name: Vain
- first_name: Eduardo
  full_name: Mateo-Bonmatí, Eduardo
  last_name: Mateo-Bonmatí
- first_name: Michal
  full_name: Karady, Michal
  last_name: Karady
- first_name: Markéta
  full_name: Pernisová, Markéta
  last_name: Pernisová
- first_name: Lenka
  full_name: Plačková, Lenka
  last_name: Plačková
- first_name: Korawit
  full_name: Opassathian, Korawit
  last_name: Opassathian
- first_name: Jan
  full_name: Hejátko, Jan
  last_name: Hejátko
- first_name: Stéphanie
  full_name: Robert, Stéphanie
  last_name: Robert
- first_name: Jiří
  full_name: Friml, Jiří
  id: 4159519E-F248-11E8-B48F-1D18A9856A87
  last_name: Friml
  orcid: 0000-0002-8302-7596
- first_name: Karel
  full_name: Doležal, Karel
  last_name: Doležal
- first_name: Karin
  full_name: Ljung, Karin
  last_name: Ljung
- first_name: Colin
  full_name: Turnbull, Colin
  last_name: Turnbull
citation:
  ama: Antoniadi I, Novák O, Gelová Z, et al. Cell-surface receptors enable perception
    of extracellular cytokinins. <i>Nature Communications</i>. 2020;11. doi:<a href="https://doi.org/10.1038/s41467-020-17700-9">10.1038/s41467-020-17700-9</a>
  apa: Antoniadi, I., Novák, O., Gelová, Z., Johnson, A. J., Plíhal, O., Simerský,
    R., … Turnbull, C. (2020). Cell-surface receptors enable perception of extracellular
    cytokinins. <i>Nature Communications</i>. Springer Nature. <a href="https://doi.org/10.1038/s41467-020-17700-9">https://doi.org/10.1038/s41467-020-17700-9</a>
  chicago: Antoniadi, Ioanna, Ondřej Novák, Zuzana Gelová, Alexander J Johnson, Ondřej
    Plíhal, Radim Simerský, Václav Mik, et al. “Cell-Surface Receptors Enable Perception
    of Extracellular Cytokinins.” <i>Nature Communications</i>. Springer Nature, 2020.
    <a href="https://doi.org/10.1038/s41467-020-17700-9">https://doi.org/10.1038/s41467-020-17700-9</a>.
  ieee: I. Antoniadi <i>et al.</i>, “Cell-surface receptors enable perception of extracellular
    cytokinins,” <i>Nature Communications</i>, vol. 11. Springer Nature, 2020.
  ista: Antoniadi I, Novák O, Gelová Z, Johnson AJ, Plíhal O, Simerský R, Mik V, Vain
    T, Mateo-Bonmatí E, Karady M, Pernisová M, Plačková L, Opassathian K, Hejátko
    J, Robert S, Friml J, Doležal K, Ljung K, Turnbull C. 2020. Cell-surface receptors
    enable perception of extracellular cytokinins. Nature Communications. 11, 4284.
  mla: Antoniadi, Ioanna, et al. “Cell-Surface Receptors Enable Perception of Extracellular
    Cytokinins.” <i>Nature Communications</i>, vol. 11, 4284, Springer Nature, 2020,
    doi:<a href="https://doi.org/10.1038/s41467-020-17700-9">10.1038/s41467-020-17700-9</a>.
  short: I. Antoniadi, O. Novák, Z. Gelová, A.J. Johnson, O. Plíhal, R. Simerský,
    V. Mik, T. Vain, E. Mateo-Bonmatí, M. Karady, M. Pernisová, L. Plačková, K. Opassathian,
    J. Hejátko, S. Robert, J. Friml, K. Doležal, K. Ljung, C. Turnbull, Nature Communications
    11 (2020).
date_created: 2020-09-06T22:01:13Z
date_published: 2020-08-27T00:00:00Z
date_updated: 2023-08-22T09:10:32Z
day: '27'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1038/s41467-020-17700-9
ec_funded: 1
external_id:
  isi:
  - '000567931000001'
file:
- access_level: open_access
  checksum: 5b96f39b598de7510cfefefb819b9a6d
  content_type: application/pdf
  creator: dernst
  date_created: 2020-12-10T12:23:56Z
  date_updated: 2020-12-10T12:23:56Z
  file_id: '8936'
  file_name: 2020_NatureComm_Antoniadi.pdf
  file_size: 3526415
  relation: main_file
  success: 1
file_date_updated: 2020-12-10T12:23:56Z
has_accepted_license: '1'
intvolume: '        11'
isi: 1
language:
- iso: eng
month: '08'
oa: 1
oa_version: Published Version
project:
- _id: 26538374-B435-11E9-9278-68D0E5697425
  call_identifier: FWF
  grant_number: I03630
  name: Molecular mechanisms of endocytic cargo recognition in plants
- _id: 261099A6-B435-11E9-9278-68D0E5697425
  call_identifier: H2020
  grant_number: '742985'
  name: Tracing Evolution of Auxin Transport and Polarity in Plants
publication: Nature Communications
publication_identifier:
  eissn:
  - '20411723'
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
status: public
title: Cell-surface receptors enable perception of extracellular cytokinins
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 11
year: '2020'
...
---
_id: '6259'
abstract:
- lang: eng
  text: The plant hormone auxin has crucial roles in almost all aspects of plant growth
    and development. Concentrations of auxin vary across different tissues, mediating
    distinct developmental outcomes and contributing to the functional diversity of
    auxin. However, the mechanisms that underlie these activities are poorly understood.
    Here we identify an auxin signalling mechanism, which acts in parallel to the
    canonical auxin pathway based on the transport inhibitor response1 (TIR1) and
    other auxin receptor F-box (AFB) family proteins (TIR1/AFB receptors)1,2, that
    translates levels of cellular auxin to mediate differential growth during apical-hook
    development. This signalling mechanism operates at the concave side of the apical
    hook, and involves auxin-mediated C-terminal cleavage of transmembrane kinase
    1 (TMK1). The cytosolic and nucleus-translocated C terminus of TMK1 specifically
    interacts with and phosphorylates two non-canonical transcriptional repressors
    of the auxin or indole-3-acetic acid (Aux/IAA) family (IAA32 and IAA34), thereby
    regulating ARF transcription factors. In contrast to the degradation of Aux/IAA
    transcriptional repressors in the canonical pathway, the newly identified mechanism
    stabilizes the non-canonical IAA32 and IAA34 transcriptional repressors to regulate
    gene expression and ultimately inhibit growth. The auxin–TMK1 signalling pathway
    originates at the cell surface, is triggered by high levels of auxin and shares
    a partially overlapping set of transcription factors with the TIR1/AFB signalling
    pathway. This allows distinct interpretations of different concentrations of cellular
    auxin, and thus enables this versatile signalling molecule to mediate complex
    developmental outcomes.
article_processing_charge: No
article_type: original
author:
- first_name: Min
  full_name: Cao, Min
  last_name: Cao
- first_name: Rong
  full_name: Chen, Rong
  last_name: Chen
- first_name: Pan
  full_name: Li, Pan
  last_name: Li
- first_name: Yongqiang
  full_name: Yu, Yongqiang
  last_name: Yu
- first_name: Rui
  full_name: Zheng, Rui
  last_name: Zheng
- first_name: Danfeng
  full_name: Ge, Danfeng
  last_name: Ge
- first_name: Wei
  full_name: Zheng, Wei
  last_name: Zheng
- first_name: Xuhui
  full_name: Wang, Xuhui
  last_name: Wang
- first_name: Yangtao
  full_name: Gu, Yangtao
  last_name: Gu
- first_name: Zuzana
  full_name: Gelová, Zuzana
  id: 0AE74790-0E0B-11E9-ABC7-1ACFE5697425
  last_name: Gelová
  orcid: 0000-0003-4783-1752
- first_name: Jiří
  full_name: Friml, Jiří
  id: 4159519E-F248-11E8-B48F-1D18A9856A87
  last_name: Friml
  orcid: 0000-0002-8302-7596
- first_name: Heng
  full_name: Zhang, Heng
  last_name: Zhang
- first_name: Renyi
  full_name: Liu, Renyi
  last_name: Liu
- first_name: Jun
  full_name: He, Jun
  last_name: He
- first_name: Tongda
  full_name: Xu, Tongda
  last_name: Xu
citation:
  ama: Cao M, Chen R, Li P, et al. TMK1-mediated auxin signalling regulates differential
    growth of the apical hook. <i>Nature</i>. 2019;568:240-243. doi:<a href="https://doi.org/10.1038/s41586-019-1069-7">10.1038/s41586-019-1069-7</a>
  apa: Cao, M., Chen, R., Li, P., Yu, Y., Zheng, R., Ge, D., … Xu, T. (2019). TMK1-mediated
    auxin signalling regulates differential growth of the apical hook. <i>Nature</i>.
    Springer Nature. <a href="https://doi.org/10.1038/s41586-019-1069-7">https://doi.org/10.1038/s41586-019-1069-7</a>
  chicago: Cao, Min, Rong Chen, Pan Li, Yongqiang Yu, Rui Zheng, Danfeng Ge, Wei Zheng,
    et al. “TMK1-Mediated Auxin Signalling Regulates Differential Growth of the Apical
    Hook.” <i>Nature</i>. Springer Nature, 2019. <a href="https://doi.org/10.1038/s41586-019-1069-7">https://doi.org/10.1038/s41586-019-1069-7</a>.
  ieee: M. Cao <i>et al.</i>, “TMK1-mediated auxin signalling regulates differential
    growth of the apical hook,” <i>Nature</i>, vol. 568. Springer Nature, pp. 240–243,
    2019.
  ista: Cao M, Chen R, Li P, Yu Y, Zheng R, Ge D, Zheng W, Wang X, Gu Y, Gelová Z,
    Friml J, Zhang H, Liu R, He J, Xu T. 2019. TMK1-mediated auxin signalling regulates
    differential growth of the apical hook. Nature. 568, 240–243.
  mla: Cao, Min, et al. “TMK1-Mediated Auxin Signalling Regulates Differential Growth
    of the Apical Hook.” <i>Nature</i>, vol. 568, Springer Nature, 2019, pp. 240–43,
    doi:<a href="https://doi.org/10.1038/s41586-019-1069-7">10.1038/s41586-019-1069-7</a>.
  short: M. Cao, R. Chen, P. Li, Y. Yu, R. Zheng, D. Ge, W. Zheng, X. Wang, Y. Gu,
    Z. Gelová, J. Friml, H. Zhang, R. Liu, J. He, T. Xu, Nature 568 (2019) 240–243.
date_created: 2019-04-09T08:37:05Z
date_published: 2019-04-11T00:00:00Z
date_updated: 2023-09-05T14:58:41Z
day: '11'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1038/s41586-019-1069-7
ec_funded: 1
external_id:
  isi:
  - '000464412700050'
  pmid:
  - '30944466'
file:
- access_level: open_access
  checksum: 6b84ab602a34382cf0340a37a1378c75
  content_type: application/pdf
  creator: dernst
  date_created: 2020-11-13T07:37:41Z
  date_updated: 2020-11-13T07:37:41Z
  file_id: '8751'
  file_name: 2019_Nature _Cao_accepted.pdf
  file_size: 4321328
  relation: main_file
  success: 1
file_date_updated: 2020-11-13T07:37:41Z
has_accepted_license: '1'
intvolume: '       568'
isi: 1
language:
- iso: eng
month: '04'
oa: 1
oa_version: Submitted Version
page: 240-243
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
  call_identifier: H2020
  grant_number: '742985'
  name: Tracing Evolution of Auxin Transport and Polarity in Plants
publication: Nature
publication_identifier:
  eissn:
  - 1476-4687
  issn:
  - 0028-0836
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
related_material:
  link:
  - description: News on IST Homepage
    relation: press_release
    url: https://ist.ac.at/en/news/newly-discovered-mechanism-of-plant-hormone-auxin-acts-the-opposite-way/
scopus_import: '1'
status: public
title: TMK1-mediated auxin signalling regulates differential growth of the apical
  hook
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 568
year: '2019'
...