---
_id: '4166'
abstract:
- lang: eng
text: In a large scale screen for mutants with defects in the embryonic development
of the zebrafish we identified mutations in four genes, floating head (flh), memo
(mom), no tail (ntl), and dec, that are required for early notochord formation.
Mutations in flh and ntl have been described previously, while mom and doe are
newly identified genes. Mutant mom embryos lack a notochord in the trunk, and
trunk somites from the right and left side of the embryo fuse underneath the neural
tube. In this respect morn appears similar to flh. In contrast, notochord precursor
cells are present in both ntl and doc embryos. In order to gain a greater understanding
of the phenotypes, we have analysed the expression of several axial mesoderm markers
in mutant embryos of all four genes. In flh and mom, Ntl expression is normal
in the germ ring and tailbud, while the expression of Nd and other notochord markers
in the axial mesodermal region is disrupted. Nd expression is normal in doc embryos
until early semitic stages, when there is a reduction in expression which is first
seen in anterior regions of the embryo. This suggests a function for doc in the
maintenance of ntl expression. Other notochord markers such as twist, sonic hedgehog
and axial are not expressed in the axial mesoderm of ntl embryos, their expression
parallels the expression of ntl in the axial mesoderm of mutant doc,flh and mom
embryos, indicating that ntl is required for the expression of these markers.
The role of doc in the expression of the notochord markers appears indirect via
ntl. Floor plate formation is disrupted in most regions in flh and mom mutant
embryos but is present in mutant ntl and doc embryos. In mutant embryos with strong
ntl alleles the band of cells expressing floor plate markers is broadened. A similar
broadening is also observed in the axial mesoderm underlying the floor plate of
ntl embryos, suggesting a direct involvement of the notochord precursor cells
in floor plate induction. Mutations in al of these four genes result in embryos
lacking a horizontal myoseptum and muscle pioneer cells, both of which are thought
to be induced by the notochord. These somite defects can be traced back to an
impairment of the specification of the adaxial cells during early stages of development.
Transplantation of wild-type cells into mutant doc embryos reveals that wild-type
notochord cells are sufficient to induce horizontal myoseptum formation in the
flanking mutant tissue. Thus dec, like flh and ntl, acts cell autonomously in
the notochord. In addition to the four mutants with defects in early notochord
formation, we have isolated 84 mutants, defining at least 15 genes, with defects
in later stages of notochord development. These are listed in an appendix to this
study.
acknowledgement: We thank Bob Riggleman for providing the twist probe prior to publication,
William Talbot, Anne Melby, Marnie Halpern and Chuck Kimmel for communicating results
prior to publication, Bill Trevarrow for the flhn1 allele, Stefan Schulte-Merker
for providing the ntl antibody, and N. H. Patel for providing the Eng antibody (4D9).
We thank Klaus Trummler, Frank Uhlmann and Mathias Metz for assistance in the analysis
of the ntl alleles, Silke Rudolph for technical assistance, Heike Schauerte for
helping with the in situ hybridization, and Joel Wilson and Cornelia Fricke for
their help with the fish work, and finally Tanya Whitfield, Francisco Pelegri, Darren
Gilmour and Stefan Schulte-Merker for discussion and help with the manuscript.
article_processing_charge: No
article_type: original
author:
- first_name: Jörg
full_name: Odenthal, Jörg
last_name: Odenthal
- first_name: Pascal
full_name: Haffter, Pascal
last_name: Haffter
- first_name: Elisabeth
full_name: Vogelsang, Elisabeth
last_name: Vogelsang
- first_name: Michael
full_name: Brand, Michael
last_name: Brand
- first_name: Fredericus
full_name: Van Eeden, Fredericus
last_name: Van Eeden
- first_name: Makoto
full_name: Furutani Seiki, Makoto
last_name: Furutani Seiki
- first_name: Michael
full_name: Granato, Michael
last_name: Granato
- first_name: Matthias
full_name: Hammerschmidt, Matthias
last_name: Hammerschmidt
- first_name: Carl-Philipp J
full_name: Heisenberg, Carl-Philipp J
id: 39427864-F248-11E8-B48F-1D18A9856A87
last_name: Heisenberg
orcid: 0000-0002-0912-4566
- first_name: Yunjin
full_name: Jiang, Yunjin
last_name: Jiang
- first_name: Donald
full_name: Kane, Donald
last_name: Kane
- first_name: Robert
full_name: Kelsh, Robert
last_name: Kelsh
- first_name: Mary
full_name: Mullins, Mary
last_name: Mullins
- first_name: Rachel
full_name: Warga, Rachel
last_name: Warga
- first_name: Miguel
full_name: Allende, Miguel
last_name: Allende
- first_name: Eric
full_name: Weinberg, Eric
last_name: Weinberg
- first_name: Christiane
full_name: Nüsslein Volhard, Christiane
last_name: Nüsslein Volhard
citation:
ama: Odenthal J, Haffter P, Vogelsang E, et al. Mutations affecting the formation
of the notochord in the zebrafish, Danio rerio. Development. 1996;123(1):103-115.
doi:10.1242/dev.123.1.103
apa: Odenthal, J., Haffter, P., Vogelsang, E., Brand, M., Van Eeden, F., Furutani
Seiki, M., … Nüsslein Volhard, C. (1996). Mutations affecting the formation of
the notochord in the zebrafish, Danio rerio. Development. Company of Biologists.
https://doi.org/10.1242/dev.123.1.103
chicago: Odenthal, Jörg, Pascal Haffter, Elisabeth Vogelsang, Michael Brand, Fredericus
Van Eeden, Makoto Furutani Seiki, Michael Granato, et al. “Mutations Affecting
the Formation of the Notochord in the Zebrafish, Danio Rerio.” Development.
Company of Biologists, 1996. https://doi.org/10.1242/dev.123.1.103.
ieee: J. Odenthal et al., “Mutations affecting the formation of the notochord
in the zebrafish, Danio rerio,” Development, vol. 123, no. 1. Company of
Biologists, pp. 103–115, 1996.
ista: Odenthal J, Haffter P, Vogelsang E, Brand M, Van Eeden F, Furutani Seiki M,
Granato M, Hammerschmidt M, Heisenberg C-PJ, Jiang Y, Kane D, Kelsh R, Mullins
M, Warga R, Allende M, Weinberg E, Nüsslein Volhard C. 1996. Mutations affecting
the formation of the notochord in the zebrafish, Danio rerio. Development. 123(1),
103–115.
mla: Odenthal, Jörg, et al. “Mutations Affecting the Formation of the Notochord
in the Zebrafish, Danio Rerio.” Development, vol. 123, no. 1, Company of
Biologists, 1996, pp. 103–15, doi:10.1242/dev.123.1.103.
short: J. Odenthal, P. Haffter, E. Vogelsang, M. Brand, F. Van Eeden, M. Furutani
Seiki, M. Granato, M. Hammerschmidt, C.-P.J. Heisenberg, Y. Jiang, D. Kane, R.
Kelsh, M. Mullins, R. Warga, M. Allende, E. Weinberg, C. Nüsslein Volhard, Development
123 (1996) 103–115.
date_created: 2018-12-11T12:07:21Z
date_published: 1996-12-01T00:00:00Z
date_updated: 2022-08-08T08:06:12Z
day: '01'
doi: 10.1242/dev.123.1.103
extern: '1'
external_id:
pmid:
- '9007233'
intvolume: ' 123'
issue: '1'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://journals.biologists.com/dev/article/123/1/103/39325/Mutations-affecting-the-formation-of-the-notochord
month: '12'
oa: 1
oa_version: Published Version
page: 103 - 115
pmid: 1
publication: Development
publication_identifier:
issn:
- 0950-1991
publication_status: published
publisher: Company of Biologists
publist_id: '1954'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Mutations affecting the formation of the notochord in the zebrafish, Danio
rerio
type: journal_article
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 123
year: '1996'
...
---
_id: '4215'
abstract:
- lang: eng
text: In a screen for early developmental mutants of the zebrafish, we have identified
mutations specifically affecting the internal organs, We identified 53 mutations
affecting the cardiovascular system, Nine of them affect specific landmarks of
heart morphogenesis. Mutations in four genes cause a failure in the fusion of
the bilateral heart primordia, resulting in cardia bifida. In lonely atrium, no
heart venticle is visible and the atrium is directly fused to the outflow tract.
In the overlooped mutant, the relative position of the two heart chambers is distorted,
The heart is enormously enlarged in the santa mutant, In two mutants, scotch tape
and superglue, the cardiac jelly between the two layers of the heart is significantly
reduced, We also identified a number of mutations affecting the function of the
heart, The mutations affecting heart function can be subdivided into two groups,
one affecting heart contraction and another affecting the rhythm of the heart
beat. Among the contractility group of mutants are 5 with no heart beat at all
and 15 with a reduced heart beat of one or both chambers, 6 mutations are in the
rhythmicity group and specifically affect the beating pattern of the heart, Mutations
in two genes, bypass and kurzschluss, cause specific defects in the circulatory
system, In addition to the heart mutants, we identified 23 mutations affecting
the integrity of the liver, the intestine or the kidney, In this report, we demonstrate
that it is feasible to screen for genes specific for the patterning or function
of certain internal organs in the zebrafish, The mutations presented here could
serve as an entrypoint to the establishment of a genetic hierarchy underlying
organogenesis.
acknowledgement: We thank Chris Simpson and Colleen Boggs for excellent technical
help. We thank Mark C. Fishman for the advice and providing fish for complementation;
Bernadette Fouquet, Kerri S. Warren and Brant M. Weinstein for critically reading
the manuscript. JNC is supported in part by NIH grant RO1-HL49579 to Mark C. Fishman.
article_processing_charge: No
article_type: original
author:
- first_name: Jaunian
full_name: Chen, Jaunian
last_name: Chen
- first_name: Pascal
full_name: Haffter, Pascal
last_name: Haffter
- first_name: Jörg
full_name: Odenthal, Jörg
last_name: Odenthal
- first_name: Elisabeth
full_name: Vogelsang, Elisabeth
last_name: Vogelsang
- first_name: Michael
full_name: Brand, Michael
last_name: Brand
- first_name: Fredericus
full_name: Van Eeden, Fredericus
last_name: Van Eeden
- first_name: Makoto
full_name: Furutani Seiki, Makoto
last_name: Furutani Seiki
- first_name: Michael
full_name: Granato, Michael
last_name: Granato
- first_name: Matthias
full_name: Hammerschmidt, Matthias
last_name: Hammerschmidt
- first_name: Carl-Philipp J
full_name: Heisenberg, Carl-Philipp J
id: 39427864-F248-11E8-B48F-1D18A9856A87
last_name: Heisenberg
orcid: 0000-0002-0912-4566
- first_name: Yunjin
full_name: Jiang, Yunjin
last_name: Jiang
- first_name: Donald
full_name: Kane, Donald
last_name: Kane
- first_name: Robert
full_name: Kelsh, Robert
last_name: Kelsh
- first_name: Mary
full_name: Mullins, Mary
last_name: Mullins
- first_name: Christiane
full_name: Nüsslein Volhard, Christiane
last_name: Nüsslein Volhard
citation:
ama: Chen J, Haffter P, Odenthal J, et al. Mutations affecting the cardiovascular
system and other internal organs in zebrafish. Development. 1996;123:293-302.
doi:10.1242/dev.123.1.293
apa: Chen, J., Haffter, P., Odenthal, J., Vogelsang, E., Brand, M., Van Eeden, F.,
… Nüsslein Volhard, C. (1996). Mutations affecting the cardiovascular system and
other internal organs in zebrafish. Development. Company of Biologists.
https://doi.org/10.1242/dev.123.1.293
chicago: Chen, Jaunian, Pascal Haffter, Jörg Odenthal, Elisabeth Vogelsang, Michael
Brand, Fredericus Van Eeden, Makoto Furutani Seiki, et al. “Mutations Affecting
the Cardiovascular System and Other Internal Organs in Zebrafish.” Development.
Company of Biologists, 1996. https://doi.org/10.1242/dev.123.1.293.
ieee: J. Chen et al., “Mutations affecting the cardiovascular system and
other internal organs in zebrafish,” Development, vol. 123. Company of
Biologists, pp. 293–302, 1996.
ista: Chen J, Haffter P, Odenthal J, Vogelsang E, Brand M, Van Eeden F, Furutani
Seiki M, Granato M, Hammerschmidt M, Heisenberg C-PJ, Jiang Y, Kane D, Kelsh R,
Mullins M, Nüsslein Volhard C. 1996. Mutations affecting the cardiovascular system
and other internal organs in zebrafish. Development. 123, 293–302.
mla: Chen, Jaunian, et al. “Mutations Affecting the Cardiovascular System and Other
Internal Organs in Zebrafish.” Development, vol. 123, Company of Biologists,
1996, pp. 293–302, doi:10.1242/dev.123.1.293.
short: J. Chen, P. Haffter, J. Odenthal, E. Vogelsang, M. Brand, F. Van Eeden, M.
Furutani Seiki, M. Granato, M. Hammerschmidt, C.-P.J. Heisenberg, Y. Jiang, D.
Kane, R. Kelsh, M. Mullins, C. Nüsslein Volhard, Development 123 (1996) 293–302.
date_created: 2018-12-11T12:07:38Z
date_published: 1996-12-01T00:00:00Z
date_updated: 2022-08-04T13:11:56Z
day: '01'
doi: 10.1242/dev.123.1.293
extern: '1'
external_id:
pmid:
- '9007249'
intvolume: ' 123'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://journals.biologists.com/dev/article/123/1/293/39344/Mutations-affecting-the-cardiovascular-system-and
month: '12'
oa: 1
oa_version: Published Version
page: 293 - 302
pmid: 1
publication: Development
publication_identifier:
issn:
- 0950-1991
publication_status: published
publisher: Company of Biologists
publist_id: '1902'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Mutations affecting the cardiovascular system and other internal organs in
zebrafish
type: journal_article
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 123
year: '1996'
...
---
_id: '4216'
abstract:
- lang: eng
text: Tissues of the dorsal midline of vertebrate embryos, such as notochord and
floor plate, have been implicated in inductive interactions that pattern the neural
tube and somites. In our screen for embryonic visible mutations in the zebrafish
we found 113 mutations in more than 27 genes with altered body shape, often with
additional defects in CNS development. We concentrated on a subgroup of mutations
in ten genes (the midline-group) that cause defective development of the floor
plate. By using floor plate markers, such as the signaling molecule sonic hedgehog,
we show that the schmalspur (sur) gene is needed for early floor plate development,
similar to one-eyed-pinhead (oep) and the previously described cyclops (eye) gene.
In contrast to oep and cyc, sur embryos show deletions of ventral CNS tissue restricted
to the mid- and hindbrain, whereas the forebrain appears largely unaffected. In
the underlying mesendodermal tissue of the head, sur is needed only for development
of the posterior prechordal plate, whereas oep and eye are required for both anterior
and posterior prechordal plate development. Our analysis of sur mutants suggests
that defects within the posterior prechordal plate may cause aberrant development
of ventral CNS structures in the mid- and hindbrain. Later development of the
floor plate is affected in mutant chameleon, you-too, sonic-you, iguana, detour,
schmalkars and monorail embryos; these mutants often show additional defects in
tissues that are known to depend on signals from notochord and floor plate, For
example, sur, con, and yot mutants show reduction of motor neurons; median deletions
of brain tissue are seen in sur, con and yot embryos; and eye, con, yet, igu and
dtr mutants often show no or abnormal formation of the optic chiasm. We also find
fusions of the ventral neurocranium for all midline mutants tested, which may
reveal a hitherto unrecognized function of the midline in influencing differentiation
of neural crest cells at their destination. As a working hypothesis, we propose
that midline-group genes may act to maintain proper structure and inductive function
of zebrafish midline tissues.
acknowledgement: "We would like to thank our colleagues in the zebrafish community
for generously sharing antibodies and probes, in particular PhilIngham, Stefan Krauss
and Vladimir Korzh, as well as Tom Jessel, Trevor Jowett, Anders Molven, Eric Weinberg
and Monte Westerfield. M. B. thanks Steve Wilson for comments on the manuscript,
his colleagues at the institute for numerous discussions, Inge Zimmermann for patient
sectioning, and Silke Hein for help during the final\r\nstages of this work. M.
B. was supported by a Helmholtz stipend of the BMFT."
article_processing_charge: No
article_type: original
author:
- first_name: Michael
full_name: Brand, Michael
last_name: Brand
- first_name: Carl-Philipp J
full_name: Heisenberg, Carl-Philipp J
id: 39427864-F248-11E8-B48F-1D18A9856A87
last_name: Heisenberg
orcid: 0000-0002-0912-4566
- first_name: Rachel
full_name: Warga, Rachel
last_name: Warga
- first_name: Francisco
full_name: Pelegri, Francisco
last_name: Pelegri
- first_name: Rolf
full_name: Karlstrom, Rolf
last_name: Karlstrom
- first_name: Dirk
full_name: Beuchle, Dirk
last_name: Beuchle
- first_name: Alexander
full_name: Picker, Alexander
last_name: Picker
- first_name: Yunjin
full_name: Jiang, Yunjin
last_name: Jiang
- first_name: Makoto
full_name: Furutani Seiki, Makoto
last_name: Furutani Seiki
- first_name: Fredericus
full_name: Van Eeden, Fredericus
last_name: Van Eeden
- first_name: Michael
full_name: Granato, Michael
last_name: Granato
- first_name: Pascal
full_name: Haffter, Pascal
last_name: Haffter
- first_name: Matthias
full_name: Hammerschmidt, Matthias
last_name: Hammerschmidt
- first_name: Donald
full_name: Kane, Donald
last_name: Kane
- first_name: Robert
full_name: Kelsh, Robert
last_name: Kelsh
- first_name: Mary
full_name: Mullins, Mary
last_name: Mullins
- first_name: Jörg
full_name: Odenthal, Jörg
last_name: Odenthal
- first_name: Christiane
full_name: Nüsslein Volhard, Christiane
last_name: Nüsslein Volhard
citation:
ama: Brand M, Heisenberg C-PJ, Warga R, et al. Mutations affecting development of
the midline and general body shape during zebrafish embryogenesis. Development.
1996;123(1):129-142. doi:10.1242/dev.123.1.129
apa: Brand, M., Heisenberg, C.-P. J., Warga, R., Pelegri, F., Karlstrom, R., Beuchle,
D., … Nüsslein Volhard, C. (1996). Mutations affecting development of the midline
and general body shape during zebrafish embryogenesis. Development. Company
of Biologists. https://doi.org/10.1242/dev.123.1.129
chicago: Brand, Michael, Carl-Philipp J Heisenberg, Rachel Warga, Francisco Pelegri,
Rolf Karlstrom, Dirk Beuchle, Alexander Picker, et al. “Mutations Affecting Development
of the Midline and General Body Shape during Zebrafish Embryogenesis.” Development.
Company of Biologists, 1996. https://doi.org/10.1242/dev.123.1.129
.
ieee: M. Brand et al., “Mutations affecting development of the midline and
general body shape during zebrafish embryogenesis,” Development, vol. 123,
no. 1. Company of Biologists, pp. 129–142, 1996.
ista: Brand M, Heisenberg C-PJ, Warga R, Pelegri F, Karlstrom R, Beuchle D, Picker
A, Jiang Y, Furutani Seiki M, Van Eeden F, Granato M, Haffter P, Hammerschmidt
M, Kane D, Kelsh R, Mullins M, Odenthal J, Nüsslein Volhard C. 1996. Mutations
affecting development of the midline and general body shape during zebrafish embryogenesis.
Development. 123(1), 129–142.
mla: Brand, Michael, et al. “Mutations Affecting Development of the Midline and
General Body Shape during Zebrafish Embryogenesis.” Development, vol. 123,
no. 1, Company of Biologists, 1996, pp. 129–42, doi:10.1242/dev.123.1.129 .
short: M. Brand, C.-P.J. Heisenberg, R. Warga, F. Pelegri, R. Karlstrom, D. Beuchle,
A. Picker, Y. Jiang, M. Furutani Seiki, F. Van Eeden, M. Granato, P. Haffter,
M. Hammerschmidt, D. Kane, R. Kelsh, M. Mullins, J. Odenthal, C. Nüsslein Volhard,
Development 123 (1996) 129–142.
date_created: 2018-12-11T12:07:38Z
date_published: 1996-12-01T00:00:00Z
date_updated: 2022-08-04T12:55:13Z
day: '01'
doi: '10.1242/dev.123.1.129 '
extern: '1'
external_id:
pmid:
- '9007235 '
intvolume: ' 123'
issue: '1'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://journals.biologists.com/dev/article/123/1/129/39318/Mutations-affecting-development-of-the-midline-and
month: '12'
oa: 1
oa_version: Published Version
page: 129 - 142
pmid: 1
publication: Development
publication_identifier:
issn:
- 0950-1991
publication_status: published
publisher: Company of Biologists
publist_id: '1900'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Mutations affecting development of the midline and general body shape during
zebrafish embryogenesis
type: journal_article
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 123
year: '1996'
...
---
_id: '4219'
abstract:
- lang: eng
text: 'Mutations in two genes affect the formation of the boundary between midbrain
and hindbrain (MHB): no isthmus (noi) and acerebellar (ace), noi mutant embryos
lack the MHB constriction, the cerebellum and optic tectum, as well as the pronephric
duct. Analysis of noi mutant embryos with neuron-specific antibodies shows that
the MHB region and the dorsal and ventral midbrain are absent or abnormal, but
that the rostral hindbrain is unaffected with the exception of the cerebellum,
Using markers that are expressed during its formation (eng, wnt1 and pax-b), we
find that the MHB region is already misspecified in noi mutant embryos during
late gastrulation. The tectum is initially present and later degenerates, The
defect in ace mutant embryos is more restricted: MHB and cerebellum are absent,
but a tectum is formed, Molecular organisation of the tectum and tegmentum is
disturbed, however, since eng, wntl and pax-b marker gene expression is not maintained,
We propose that noi and ace are required for development of the MHB region and
of the adjacent mid- and hindbrain, which are thought to be patterned by the MHB
region, Presence of pax-b RNA, and absence of pax-b protein, together with the
observation of genetic linkage and the occurrence of a point mutation, show that
noi mutations are located in the pax-b gene, pax-b is a vertebrate orthologue
of the Drosophila gene paired, which is involved in a pathway of cellular interactions
at the posterior compartment boundary in Drosophila, Our results confirm and extend
a previous report, and show that at least one member of this conserved signalling
pathway is required for formation of the boundary between midbrain and hindbrain
in the zebrafish.'
acknowledgement: "We would like to thank our colleagues in the zebrafish community
for generously sharing antibodies and probes, in particular Terje Johannsen, Vladimir
Korzh, Stefan Krauss and Ingvild Mikkola, as well as Christine Dreyer, Nigel Holder,
Tom Jessel, Trevor Jowett, Anders Molven, Eric Weinberg and Monte Westerfield. M.B
would like to thank his colleagues for numerous discussions, and Francisco Pelegri,
Suresh Jesuthasan and Luis Puelles for comments on the\r\nmanuscipt. Thanks also
to Peter Andermann and Eric Weinberg, who helped in the analysis of Zash expression,
and especially to Corinne Houart, for her lovely in situ protocol and many discussions.
Silke Hein helped greatly in final stages of this work. M.B. was supported by a
Helmholtz stipend of the BMFT."
article_processing_charge: No
article_type: original
author:
- first_name: Michael
full_name: Brand, Michael
last_name: Brand
- first_name: Carl-Philipp J
full_name: Heisenberg, Carl-Philipp J
id: 39427864-F248-11E8-B48F-1D18A9856A87
last_name: Heisenberg
orcid: 0000-0002-0912-4566
- first_name: Yunjin
full_name: Jiang, Yunjin
last_name: Jiang
- first_name: Dirk
full_name: Beuchle, Dirk
last_name: Beuchle
- first_name: Klaus
full_name: Lun, Klaus
last_name: Lun
- first_name: Makoto
full_name: Furutani Seiki, Makoto
last_name: Furutani Seiki
- first_name: Michael
full_name: Granato, Michael
last_name: Granato
- first_name: Pascal
full_name: Haffter, Pascal
last_name: Haffter
- first_name: Matthias
full_name: Hammerschmidt, Matthias
last_name: Hammerschmidt
- first_name: Donald
full_name: Kane, Donald
last_name: Kane
- first_name: Robert
full_name: Kelsh, Robert
last_name: Kelsh
- first_name: Mary
full_name: Mullins, Mary
last_name: Mullins
- first_name: Jörg
full_name: Odenthal, Jörg
last_name: Odenthal
- first_name: Fredericus
full_name: Van Eeden, Fredericus
last_name: Van Eeden
- first_name: Christiane
full_name: Nüsslein Volhard, Christiane
last_name: Nüsslein Volhard
citation:
ama: Brand M, Heisenberg C-PJ, Jiang Y, et al. Mutations in zebrafish genes affecting
the formation of the boundary between midbrain and hindbrain. Development.
1996;123(1):179-190. doi:10.1242/dev.123.1.179
apa: Brand, M., Heisenberg, C.-P. J., Jiang, Y., Beuchle, D., Lun, K., Furutani
Seiki, M., … Nüsslein Volhard, C. (1996). Mutations in zebrafish genes affecting
the formation of the boundary between midbrain and hindbrain. Development.
Company of Biologists. https://doi.org/10.1242/dev.123.1.179
chicago: Brand, Michael, Carl-Philipp J Heisenberg, Yunjin Jiang, Dirk Beuchle,
Klaus Lun, Makoto Furutani Seiki, Michael Granato, et al. “Mutations in Zebrafish
Genes Affecting the Formation of the Boundary between Midbrain and Hindbrain.”
Development. Company of Biologists, 1996. https://doi.org/10.1242/dev.123.1.179 .
ieee: M. Brand et al., “Mutations in zebrafish genes affecting the formation
of the boundary between midbrain and hindbrain,” Development, vol. 123,
no. 1. Company of Biologists, pp. 179–190, 1996.
ista: Brand M, Heisenberg C-PJ, Jiang Y, Beuchle D, Lun K, Furutani Seiki M, Granato
M, Haffter P, Hammerschmidt M, Kane D, Kelsh R, Mullins M, Odenthal J, Van Eeden
F, Nüsslein Volhard C. 1996. Mutations in zebrafish genes affecting the formation
of the boundary between midbrain and hindbrain. Development. 123(1), 179–190.
mla: Brand, Michael, et al. “Mutations in Zebrafish Genes Affecting the Formation
of the Boundary between Midbrain and Hindbrain.” Development, vol. 123,
no. 1, Company of Biologists, 1996, pp. 179–90, doi:10.1242/dev.123.1.179 .
short: M. Brand, C.-P.J. Heisenberg, Y. Jiang, D. Beuchle, K. Lun, M. Furutani Seiki,
M. Granato, P. Haffter, M. Hammerschmidt, D. Kane, R. Kelsh, M. Mullins, J. Odenthal,
F. Van Eeden, C. Nüsslein Volhard, Development 123 (1996) 179–190.
date_created: 2018-12-11T12:07:40Z
date_published: 1996-12-01T00:00:00Z
date_updated: 2022-08-04T11:45:04Z
day: '01'
doi: '10.1242/dev.123.1.179 '
extern: '1'
external_id:
pmid:
- '9007239 '
intvolume: ' 123'
issue: '1'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://journals.biologists.com/dev/article/123/1/179/39324/Mutations-in-zebrafish-genes-affecting-the
month: '12'
oa: 1
oa_version: Published Version
page: 179 - 190
pmid: 1
publication: Development
publication_identifier:
issn:
- 0950-1991
publication_status: published
publisher: Company of Biologists
publist_id: '1899'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Mutations in zebrafish genes affecting the formation of the boundary between
midbrain and hindbrain
type: journal_article
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 123
year: '1996'
...
---
_id: '4220'
abstract:
- lang: eng
text: In the zebrafish, Danio rerio, a caudal and pectoral fin fold develop during
embryogenesis. At larval stages the caudal fin fold is replaced by four different
fins, the unpaired anal, dorsal and tail fins. In addition the paired pelvic fins
are formed, We have identified a total of 118 mutations affecting larval fin formation,
Mutations in 11 genes lead to abnormal morphology or degeneration of both caudal
and pectoral fin folds, Most mutants survive to adulthood and form a surprisingly
normal complement of adult fins, Mutations in nine genes result in an increased
or reduced size of the pectoral fins, Interestingly, in mutants of one of these
genes, dackel (dak), pectoral fin buds form initially, but later the fin epithelium
fails to expand, Expression of sonic hedgehog mRNA in the posterior mesenchyme
of the pectoral fin bud is initiated in dak embryos, but not maintained, Mutations
in five other genes affect adult fin but not larval fin development, Two mutants,
longfin (lof) and another longfin (alf) have generally longer fins. Stein und
bein (sub) has reduced dorsal and pelvic fins, whereas finless (fls) and wanda
(wan) mutants affect all adult fins, Finally, mutations in four genes causing
defects in embryonic skin formation will be briefly reported.
article_processing_charge: No
article_type: original
author:
- first_name: Fredericus
full_name: Van Eeden, Fredericus
last_name: Van Eeden
- first_name: Michael
full_name: Granato, Michael
last_name: Granato
- first_name: Ursula
full_name: Schach, Ursula
last_name: Schach
- first_name: Michael
full_name: Brand, Michael
last_name: Brand
- first_name: Makoto
full_name: Furutani Seiki, Makoto
last_name: Furutani Seiki
- first_name: Pascal
full_name: Haffter, Pascal
last_name: Haffter
- first_name: Matthias
full_name: Hammerschmidt, Matthias
last_name: Hammerschmidt
- first_name: Carl-Philipp J
full_name: Heisenberg, Carl-Philipp J
id: 39427864-F248-11E8-B48F-1D18A9856A87
last_name: Heisenberg
orcid: 0000-0002-0912-4566
- first_name: Yunjin
full_name: Jiang, Yunjin
last_name: Jiang
- first_name: Donald
full_name: Kane, Donald
last_name: Kane
- first_name: Robert
full_name: Kelsh, Robert
last_name: Kelsh
- first_name: Mary
full_name: Mullins, Mary
last_name: Mullins
- first_name: Jörg
full_name: Odenthal, Jörg
last_name: Odenthal
- first_name: Rachel
full_name: Warga, Rachel
last_name: Warga
- first_name: Christiane
full_name: Nüsslein Volhard, Christiane
last_name: Nüsslein Volhard
citation:
ama: Van Eeden F, Granato M, Schach U, et al. Genetic analysis of fin formation
in the zebrafish, Danio rerio. Development. 1996;123(1):255-262. doi:10.1242/dev.123.1.255
apa: Van Eeden, F., Granato, M., Schach, U., Brand, M., Furutani Seiki, M., Haffter,
P., … Nüsslein Volhard, C. (1996). Genetic analysis of fin formation in the zebrafish,
Danio rerio. Development. Company of Biologists. https://doi.org/10.1242/dev.123.1.255
chicago: Van Eeden, Fredericus, Michael Granato, Ursula Schach, Michael Brand, Makoto
Furutani Seiki, Pascal Haffter, Matthias Hammerschmidt, et al. “Genetic Analysis
of Fin Formation in the Zebrafish, Danio Rerio.” Development. Company of
Biologists, 1996. https://doi.org/10.1242/dev.123.1.255
.
ieee: F. Van Eeden et al., “Genetic analysis of fin formation in the zebrafish,
Danio rerio,” Development, vol. 123, no. 1. Company of Biologists, pp.
255–262, 1996.
ista: Van Eeden F, Granato M, Schach U, Brand M, Furutani Seiki M, Haffter P, Hammerschmidt
M, Heisenberg C-PJ, Jiang Y, Kane D, Kelsh R, Mullins M, Odenthal J, Warga R,
Nüsslein Volhard C. 1996. Genetic analysis of fin formation in the zebrafish,
Danio rerio. Development. 123(1), 255–262.
mla: Van Eeden, Fredericus, et al. “Genetic Analysis of Fin Formation in the Zebrafish,
Danio Rerio.” Development, vol. 123, no. 1, Company of Biologists, 1996,
pp. 255–62, doi:10.1242/dev.123.1.255
.
short: F. Van Eeden, M. Granato, U. Schach, M. Brand, M. Furutani Seiki, P. Haffter,
M. Hammerschmidt, C.-P.J. Heisenberg, Y. Jiang, D. Kane, R. Kelsh, M. Mullins,
J. Odenthal, R. Warga, C. Nüsslein Volhard, Development 123 (1996) 255–262.
date_created: 2018-12-11T12:07:40Z
date_published: 1996-12-01T00:00:00Z
date_updated: 2022-08-04T10:01:17Z
day: '01'
doi: '10.1242/dev.123.1.255 '
extern: '1'
external_id:
pmid:
- '9007245 '
intvolume: ' 123'
issue: '1'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://journals.biologists.com/dev/article/123/1/255/39327/Genetic-analysis-of-fin-formation-in-the-zebrafish
month: '12'
oa: 1
oa_version: Published Version
page: 255 - 262
pmid: 1
publication: Development
publication_identifier:
issn:
- 0950-1991
publication_status: published
publisher: Company of Biologists
publist_id: '1896'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Genetic analysis of fin formation in the zebrafish, Danio rerio
type: journal_article
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 123
year: '1996'
...
---
_id: '4222'
abstract:
- lang: eng
text: Somitogenesis is the basis of segmentation of the mesoderm in the trunk and
tail of vertebrate embryos, Two groups of mutants with defects in this patterning
process have been isolated in our screen for zygotic mutations affecting the embryonic
development of the zebrafish (Danio rerio), In mutants of the first group, boundaries
between individual somites are invisible early on, although the paraxial mesoderm
is present, Later, irregular boundaries between somites are present, Mutations
infused somites (fss) and beamter (bea) affect all somites, whereas mutations
in deadly seven (des), after eight (aei) and white tail (wit) only affect the
more posterior somites, Mutants of all genes but wit are homozygous viable and
fertile, Skeletal stainings and the expression pattern of myoD and snail1 suggest
that anteroposterior patterning within individual somites is abnormal, In the
second group of mutants, formation of the horizontal myoseptum, which separates
the dorsal and ventral part of the myotome, is reduced, Six genes have been defined
in this group (you-type genes), yea-too mutants show the most severe phenotype;
in these the adaxial cells, muscle pioneers and the primary motoneurons are affected,
in addition to the horizontal myoseptum. The horizontal myoseptum is also missing
in mutants that lack a notochord. The similarity of the somite phenotype in mutants
lacking the notochord and in the you-type mutants suggests that the genes mutated
in these two groups are involved in a signaling pathway from the notochord, important
for patterning of the somites.
acknowledgement: We would like to thank P. Ingham and T. Whitfield for valuable comments
on the manuscript and cDNA probes, S. Schulte-Merker for the Ntl antibody and J.
Eisen and R. BreMiller for the znp-1 antibody.
article_processing_charge: No
article_type: original
author:
- first_name: Fredericus
full_name: Van Eeden, Fredericus
last_name: Van Eeden
- first_name: Michael
full_name: Granato, Michael
last_name: Granato
- first_name: Ursula
full_name: Schach, Ursula
last_name: Schach
- first_name: Michael
full_name: Brand, Michael
last_name: Brand
- first_name: Makoto
full_name: Furutani Seiki, Makoto
last_name: Furutani Seiki
- first_name: Pascal
full_name: Haffter, Pascal
last_name: Haffter
- first_name: Matthias
full_name: Hammerschmidt, Matthias
last_name: Hammerschmidt
- first_name: Carl-Philipp J
full_name: Heisenberg, Carl-Philipp J
id: 39427864-F248-11E8-B48F-1D18A9856A87
last_name: Heisenberg
orcid: 0000-0002-0912-4566
- first_name: Yunjin
full_name: Jiang, Yunjin
last_name: Jiang
- first_name: Donald
full_name: Kane, Donald
last_name: Kane
- first_name: Robert
full_name: Kelsh, Robert
last_name: Kelsh
- first_name: Mary
full_name: Mullins, Mary
last_name: Mullins
- first_name: Jörg
full_name: Odenthal, Jörg
last_name: Odenthal
- first_name: Rachel
full_name: Warga, Rachel
last_name: Warga
- first_name: Miguel
full_name: Allende, Miguel
last_name: Allende
- first_name: Eric
full_name: Weinberg, Eric
last_name: Weinberg
- first_name: Christiane
full_name: Nüsslein Volhard, Christiane
last_name: Nüsslein Volhard
citation:
ama: Van Eeden F, Granato M, Schach U, et al. Mutations affecting somite formation
and patterning in the zebrafish, Danio rerio. Development. 1996;123(1):153-164.
doi:10.1242/dev.123.1.153
apa: Van Eeden, F., Granato, M., Schach, U., Brand, M., Furutani Seiki, M., Haffter,
P., … Nüsslein Volhard, C. (1996). Mutations affecting somite formation and patterning
in the zebrafish, Danio rerio. Development. Company of Biologists. https://doi.org/10.1242/dev.123.1.153
chicago: Van Eeden, Fredericus, Michael Granato, Ursula Schach, Michael Brand, Makoto
Furutani Seiki, Pascal Haffter, Matthias Hammerschmidt, et al. “Mutations Affecting
Somite Formation and Patterning in the Zebrafish, Danio Rerio.” Development.
Company of Biologists, 1996. https://doi.org/10.1242/dev.123.1.153.
ieee: F. Van Eeden et al., “Mutations affecting somite formation and patterning
in the zebrafish, Danio rerio,” Development, vol. 123, no. 1. Company of
Biologists, pp. 153–164, 1996.
ista: Van Eeden F, Granato M, Schach U, Brand M, Furutani Seiki M, Haffter P, Hammerschmidt
M, Heisenberg C-PJ, Jiang Y, Kane D, Kelsh R, Mullins M, Odenthal J, Warga R,
Allende M, Weinberg E, Nüsslein Volhard C. 1996. Mutations affecting somite formation
and patterning in the zebrafish, Danio rerio. Development. 123(1), 153–164.
mla: Van Eeden, Fredericus, et al. “Mutations Affecting Somite Formation and Patterning
in the Zebrafish, Danio Rerio.” Development, vol. 123, no. 1, Company of
Biologists, 1996, pp. 153–64, doi:10.1242/dev.123.1.153.
short: F. Van Eeden, M. Granato, U. Schach, M. Brand, M. Furutani Seiki, P. Haffter,
M. Hammerschmidt, C.-P.J. Heisenberg, Y. Jiang, D. Kane, R. Kelsh, M. Mullins,
J. Odenthal, R. Warga, M. Allende, E. Weinberg, C. Nüsslein Volhard, Development
123 (1996) 153–164.
date_created: 2018-12-11T12:07:41Z
date_published: 1996-12-01T00:00:00Z
date_updated: 2022-08-04T09:29:56Z
day: '01'
doi: 10.1242/dev.123.1.153
extern: '1'
external_id:
pmid:
- '9007237 '
intvolume: ' 123'
issue: '1'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://journals.biologists.com/dev/article/123/1/153/39329/Mutations-affecting-somite-formation-and
month: '12'
oa: 1
oa_version: Published Version
page: 153 - 164
pmid: 1
publication: Development
publication_identifier:
issn:
- 0950-1991
publication_status: published
publisher: Company of Biologists
publist_id: '1895'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Mutations affecting somite formation and patterning in the zebrafish, Danio
rerio
type: journal_article
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 123
year: '1996'
...
---
_id: '4295'
abstract:
- lang: eng
text: Genetic studies are beginning to provide insights into the evolutionary processes
that reduce the fitness of hybrids between recently diverged species. However,
the deleterious gene interactions responsible for this fitness reduction are still
poorly understood.
acknowledgement: Thanks to Brian Charlesworth, Jerry Coyne, Allen Orr and Michael
Turelli for their comments on this note, and to the BBSRC and NERC for financial
support.
article_processing_charge: No
author:
- first_name: Nicholas H
full_name: Barton, Nicholas H
id: 4880FE40-F248-11E8-B48F-1D18A9856A87
last_name: Barton
orcid: 0000-0002-8548-5240
citation:
ama: 'Barton NH. Speciation: more than the sum of its parts. In: Current Biology.
Vol 6. Cell Press; 1996:1244-1246. doi:10.1016/S0960-9822(02)70707-0'
apa: 'Barton, N. H. (1996). Speciation: more than the sum of its parts. In Current
Biology (Vol. 6, pp. 1244–1246). Cell Press. https://doi.org/10.1016/S0960-9822(02)70707-0'
chicago: 'Barton, Nicholas H. “Speciation: More than the Sum of Its Parts.” In Current
Biology, 6:1244–46. Cell Press, 1996. https://doi.org/10.1016/S0960-9822(02)70707-0.'
ieee: 'N. H. Barton, “Speciation: more than the sum of its parts,” in Current
Biology, vol. 6, Cell Press, 1996, pp. 1244–1246.'
ista: 'Barton NH. 1996.Speciation: more than the sum of its parts. In: Current Biology.
vol. 6, 1244–1246.'
mla: 'Barton, Nicholas H. “Speciation: More than the Sum of Its Parts.” Current
Biology, vol. 6, Cell Press, 1996, pp. 1244–46, doi:10.1016/S0960-9822(02)70707-0.'
short: N.H. Barton, in:, Current Biology, Cell Press, 1996, pp. 1244–1246.
date_created: 2018-12-11T12:08:06Z
date_published: 1996-10-01T00:00:00Z
date_updated: 2022-07-07T09:28:28Z
day: '01'
doi: 10.1016/S0960-9822(02)70707-0
extern: '1'
external_id:
pmid:
- '8939554'
intvolume: ' 6'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://www.sciencedirect.com/science/article/pii/S0960982202707070?via%3Dihub
month: '10'
oa: 1
oa_version: Published Version
page: 1244 - 1246
pmid: 1
publication: Current Biology
publication_identifier:
issn:
- 0960-9822
publication_status: published
publisher: Cell Press
publist_id: '1781'
quality_controlled: '1'
scopus_import: '1'
status: public
title: 'Speciation: more than the sum of its parts'
type: book_chapter
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 6
year: '1996'
...
---
_id: '4611'
abstract:
- lang: eng
text: Presents a model-checking procedure and its implementation for the automatic
verification of embedded systems. The system components are described as hybrid
automata-communicating machines with finite control and real-valued variables
that represent continuous environment parameters such as time, pressure and temperature.
The system requirements are specified in a temporal logic with stop-watches, and
verified by symbolic fixpoint computation. The verification procedure-implemented
in the Cornell Hybrid Technology tool, HyTech-applies to hybrid automata whose
continuous dynamics is governed by linear constraints on the variables and their
derivatives. We illustrate the method and the tool by checking safety, liveness,
time-bounded and duration requirements of digital controllers, schedulers and
distributed algorithms
acknowledgement: "We thank Costas Courcoubetis, Nicolas Halbwachs, Peter Kopke, Joseph
Sifakis, and Howard Wong-Toi for helpful\r\ndiscussions and valuable comments. Thomas
A. Henzinger's research was supported in part by the U.S. Office of Naval Research
Young Investigator award N00014-95-1-0520, by the National Science Foundation CAREER
award CCR-9501708, by National Science Foundation grants CCR-9200794 and CCR-9504469,
by U.S. Air Force Office of Scientific Research contract F49620-93-1- 0056, and
by Advanced Research Projects Agency grant NAG2-892. "
article_processing_charge: No
article_type: original
author:
- first_name: Rajeev
full_name: Alur, Rajeev
last_name: Alur
- first_name: Thomas A
full_name: Henzinger, Thomas A
id: 40876CD8-F248-11E8-B48F-1D18A9856A87
last_name: Henzinger
orcid: 0000−0002−2985−7724
- first_name: Pei
full_name: Ho, Pei
last_name: Ho
citation:
ama: Alur R, Henzinger TA, Ho P. Automatic symbolic verification of embedded systems.
IEEE Transactions on Software Engineering. 1996;22(3):181-201. doi:10.1109/32.489079
apa: Alur, R., Henzinger, T. A., & Ho, P. (1996). Automatic symbolic verification
of embedded systems. IEEE Transactions on Software Engineering. IEEE. https://doi.org/10.1109/32.489079
chicago: Alur, Rajeev, Thomas A Henzinger, and Pei Ho. “Automatic Symbolic Verification
of Embedded Systems.” IEEE Transactions on Software Engineering. IEEE,
1996. https://doi.org/10.1109/32.489079.
ieee: R. Alur, T. A. Henzinger, and P. Ho, “Automatic symbolic verification of embedded
systems,” IEEE Transactions on Software Engineering, vol. 22, no. 3. IEEE,
pp. 181–201, 1996.
ista: Alur R, Henzinger TA, Ho P. 1996. Automatic symbolic verification of embedded
systems. IEEE Transactions on Software Engineering. 22(3), 181–201.
mla: Alur, Rajeev, et al. “Automatic Symbolic Verification of Embedded Systems.”
IEEE Transactions on Software Engineering, vol. 22, no. 3, IEEE, 1996,
pp. 181–201, doi:10.1109/32.489079.
short: R. Alur, T.A. Henzinger, P. Ho, IEEE Transactions on Software Engineering
22 (1996) 181–201.
date_created: 2018-12-11T12:09:45Z
date_published: 1996-03-01T00:00:00Z
date_updated: 2022-07-04T12:47:05Z
day: '01'
doi: 10.1109/32.489079
extern: '1'
intvolume: ' 22'
issue: '3'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://ecommons.cornell.edu/handle/1813/7170
month: '03'
oa: 1
oa_version: Published Version
page: 181 - 201
publication: IEEE Transactions on Software Engineering
publication_identifier:
issn:
- 0018-9162
publication_status: published
publisher: IEEE
publist_id: '96'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Automatic symbolic verification of embedded systems
type: journal_article
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 22
year: '1996'
...
---
_id: '11927'
abstract:
- lang: eng
text: "We are given a set 7 = {Tl , Tz, . . . , Tk} of rooted binary trees, each
Ti leaf-labeled by a subset L(x) c {1,2 )...) n}. IfT is a tree on {1,2, . . ,
n}, we let T]L denote the subtree of T induced by the nodes of L and all their
ancestors. The consensus tree problem asks whether there exists a tree T* such
that for every I, T’ IC(Ti) is homeomorphic to Ti. We present algorithms which
test if a given set of trees has a consensus tree and if so, construct one. The
deterministic algorithm takes time min{O(mn’/‘), O(m + n2 logn)}, where m = Ci
IZl and uses linear space. The randomized algorithm takes\r\ntime O(m log3 n)
and uses linear space. The previous best for this problem was an 1981 O(mn) algorithm
by Aho et al. Our faster deterministic algorithm uses a new efficient algorithm
for the following interesting dynamic graph problem: Given a graph G with n nodes
and m edges and a sequence of b batches of one or more edge deletions, then after
each batch, either find a new component that has just been created or determine
that there is no such component. For this\r\nproblem, we have a simple algorithm
with running time O(n2 log n + be min{ n2, m log n}), where be is the number of
batches which do not result in a new component. For our particular application,
bc 5 1. If all edges are deleted, then the best previously known deterministic
algorithm requires time O(mJ;ii) to solve this problem. computational evolutionary
biology. The first application is in the problem of inferring consensus of trees
when there can be disagreement[l6]. There have, been several models suggested
for this problem[2, 3, 4, 8, ?, 11, 17, 181, of which one is called the Local
Consensus Tree[l5]. The local consensus tree model presumes that the user provides
a local consensus rule which determines the form of the output tree on (perhaps)
each triple of leaves, and the objective is to determine whether a tree exists
which is consistent with each of the constraints. We will show that we can construct
the local consensus tree of k trees on n species in O(kn3) time, improving on
the O(lcn3 + n”) running time if we use the Aho et al algorithm. The second application
is a\r\nheuristic for constructing the maximum likelihood tree based upon combining
solutions to small subproblems.\r\nThis is a simple and yet potentially significantly
interesting approach to the evolutionary tree construction\r\nproblem. "
article_processing_charge: No
author:
- first_name: Monika H
full_name: Henzinger, Monika H
id: 540c9bbd-f2de-11ec-812d-d04a5be85630
last_name: Henzinger
orcid: 0000-0002-5008-6530
- first_name: Valerie
full_name: King, Valerie
last_name: King
- first_name: Tandy
full_name: Warnow, Tandy
last_name: Warnow
citation:
ama: 'Henzinger MH, King V, Warnow T. Constructing a tree from homeomorphic subtrees,
with applications to computational evolutionary biology. In: 7th Annual ACM-SIAM
Symposium on Discrete Algorithms. Society for Industrial and Applied Mathematics;
1996:333-340.'
apa: 'Henzinger, M. H., King, V., & Warnow, T. (1996). Constructing a tree from
homeomorphic subtrees, with applications to computational evolutionary biology.
In 7th Annual ACM-SIAM Symposium on Discrete Algorithms (pp. 333–340).
Atlanta, GA, United States: Society for Industrial and Applied Mathematics.'
chicago: Henzinger, Monika H, Valerie King, and Tandy Warnow. “Constructing a Tree
from Homeomorphic Subtrees, with Applications to Computational Evolutionary Biology.”
In 7th Annual ACM-SIAM Symposium on Discrete Algorithms, 333–40. Society
for Industrial and Applied Mathematics, 1996.
ieee: M. H. Henzinger, V. King, and T. Warnow, “Constructing a tree from homeomorphic
subtrees, with applications to computational evolutionary biology,” in 7th
Annual ACM-SIAM Symposium on Discrete Algorithms, Atlanta, GA, United States,
1996, pp. 333–340.
ista: 'Henzinger MH, King V, Warnow T. 1996. Constructing a tree from homeomorphic
subtrees, with applications to computational evolutionary biology. 7th Annual
ACM-SIAM Symposium on Discrete Algorithms. SODA: Symposium on Discrete Algorithms,
333–340.'
mla: Henzinger, Monika H., et al. “Constructing a Tree from Homeomorphic Subtrees,
with Applications to Computational Evolutionary Biology.” 7th Annual ACM-SIAM
Symposium on Discrete Algorithms, Society for Industrial and Applied Mathematics,
1996, pp. 333–40.
short: M.H. Henzinger, V. King, T. Warnow, in:, 7th Annual ACM-SIAM Symposium on
Discrete Algorithms, Society for Industrial and Applied Mathematics, 1996, pp.
333–340.
conference:
end_date: 1996-01-30
location: Atlanta, GA, United States
name: 'SODA: Symposium on Discrete Algorithms'
start_date: 1996-01-28
date_created: 2022-08-19T06:57:47Z
date_published: 1996-01-28T00:00:00Z
date_updated: 2023-02-21T16:24:53Z
day: '28'
extern: '1'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://dl.acm.org/doi/10.5555/313852.314080
month: '01'
oa: 1
oa_version: Published Version
page: 333 -340
publication: 7th Annual ACM-SIAM Symposium on Discrete Algorithms
publication_identifier:
isbn:
- '0898713668'
publication_status: published
publisher: Society for Industrial and Applied Mathematics
quality_controlled: '1'
related_material:
record:
- id: '11679'
relation: later_version
status: public
scopus_import: '1'
status: public
title: Constructing a tree from homeomorphic subtrees, with applications to computational
evolutionary biology
type: conference
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '1996'
...
---
_id: '1943'
abstract:
- lang: eng
text: Transhydrogenase from beef-heart mitochondria was solubilised with Triton
X-100 and purified by column chromatography. The detergent-dispersed enzyme catalysed
the reduction of acetylpyridine adenine dinucleotide (AcPdAD+) by NADH, but only
in the presence of NADP+. Experiments showed that this reaction was cyclic; NADP(H),
whilst remaining bound to the enzyme, was alternately reduced by NADH and oxidised
by AcPdAD+. A period of incubation of the enzyme with NADPH at pH 6.0 led to inhibition
of the simple transhydrogenation reaction between AcPdAD+ and NADPH. However,
after such treatment, transhydrogenase acquired the ability to catalyse the (NADPH-dependent)
reduction of AcPdAD+ by NADH. It is suggested that this is a similar cycle to
the one described above. Evidently, the binding affinity for NADP+ increases as
a consequence of the inhibition process resulting from prolonged incubation with
NADPH. The pH dependences of simple and cyclic transhydrogenation reactions are
described. Though more complex than those in Escherichia coli transhydrogenase,
they are consistent with the view [Hutton, M., Day, J.M., Bizouarn, T. and Jackson,
J.B. (1994) Eur. J. Biochem. 219, 1041–10511] that, also in the mitochondrial
enzyme, binding the release of NADP+ and NADP are accompanied by binding and release
of a proton. The enzyme was successfully reconstituted into liposomes by a cholate
dilution procedure. The proteoliposomes catalysed cyclic NADPH-dependent reduction
of AcPdAD+ by NADH only when they were tightly coupled. However, they catalysed
cyclic NADP+-dependent reduction of AcPdAD+ by NADH only when they were uncoupled
eg. by addition of carbonylcyanide-p-trifluoromethoxyphenyl hydrazone. These observations
are evidence that the proton binding and release which accompany NADP+ binding
and release, respectively, take place on the inside of the vesicle, and that they
are components of the electrogenic processes of the enzyme.
acknowledgement: 'L.A.S. is grateful to the Wellcome Trust for a Research Fellowship.
Support from the Biotechnology and Biological Sciences Research Council is also
acknowledged. We thank our colleagues. Tania Bizouarn, Mike Hutton and Nick Cotton,
for advice and valuable discussion. '
article_processing_charge: No
article_type: original
author:
- first_name: Leonid A
full_name: Sazanov, Leonid A
id: 338D39FE-F248-11E8-B48F-1D18A9856A87
last_name: Sazanov
orcid: 0000-0002-0977-7989
- first_name: Baz
full_name: Jackson, Baz
last_name: Jackson
citation:
ama: Sazanov LA, Jackson B. Cyclic reactions catalysed by detergent-dispersed and
reconstituted transhydrogenase from beef heart mitochondria; implications for
the mechanism of proton translocation. Biochimica et Biophysica Acta - Bioenergetics.
1995;1231(3):304-312. doi:10.1016/0005-2728(95)00096-2
apa: Sazanov, L. A., & Jackson, B. (1995). Cyclic reactions catalysed by detergent-dispersed
and reconstituted transhydrogenase from beef heart mitochondria; implications
for the mechanism of proton translocation. Biochimica et Biophysica Acta -
Bioenergetics. Elsevier. https://doi.org/10.1016/0005-2728(95)00096-2
chicago: Sazanov, Leonid A, and Baz Jackson. “Cyclic Reactions Catalysed by Detergent-Dispersed
and Reconstituted Transhydrogenase from Beef Heart Mitochondria; Implications
for the Mechanism of Proton Translocation.” Biochimica et Biophysica Acta -
Bioenergetics. Elsevier, 1995. https://doi.org/10.1016/0005-2728(95)00096-2.
ieee: L. A. Sazanov and B. Jackson, “Cyclic reactions catalysed by detergent-dispersed
and reconstituted transhydrogenase from beef heart mitochondria; implications
for the mechanism of proton translocation,” Biochimica et Biophysica Acta -
Bioenergetics, vol. 1231, no. 3. Elsevier, pp. 304–312, 1995.
ista: Sazanov LA, Jackson B. 1995. Cyclic reactions catalysed by detergent-dispersed
and reconstituted transhydrogenase from beef heart mitochondria; implications
for the mechanism of proton translocation. Biochimica et Biophysica Acta - Bioenergetics.
1231(3), 304–312.
mla: Sazanov, Leonid A., and Baz Jackson. “Cyclic Reactions Catalysed by Detergent-Dispersed
and Reconstituted Transhydrogenase from Beef Heart Mitochondria; Implications
for the Mechanism of Proton Translocation.” Biochimica et Biophysica Acta -
Bioenergetics, vol. 1231, no. 3, Elsevier, 1995, pp. 304–12, doi:10.1016/0005-2728(95)00096-2.
short: L.A. Sazanov, B. Jackson, Biochimica et Biophysica Acta - Bioenergetics 1231
(1995) 304–312.
date_created: 2018-12-11T11:54:50Z
date_published: 1995-10-10T00:00:00Z
date_updated: 2022-06-29T15:04:47Z
day: '10'
doi: 10.1016/0005-2728(95)00096-2
extern: '1'
external_id:
pmid:
- '7578218'
intvolume: ' 1231'
issue: '3'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://www.sciencedirect.com/science/article/pii/0005272895000962?via%3Dihub
month: '10'
oa: 1
oa_version: Published Version
page: 304 - 312
pmid: 1
publication: Biochimica et Biophysica Acta - Bioenergetics
publication_identifier:
issn:
- 0005-2728
publication_status: published
publisher: Elsevier
publist_id: '5142'
quality_controlled: '1'
status: public
title: Cyclic reactions catalysed by detergent-dispersed and reconstituted transhydrogenase
from beef heart mitochondria; implications for the mechanism of proton translocation
type: journal_article
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 1231
year: '1995'
...
---
_id: '3461'
article_processing_charge: No
article_type: original
author:
- first_name: Peter M
full_name: Jonas, Peter M
id: 353C1B58-F248-11E8-B48F-1D18A9856A87
last_name: Jonas
orcid: 0000-0001-5001-4804
- first_name: Nail
full_name: Burnashev, Nail
last_name: Burnashev
citation:
ama: Jonas PM, Burnashev N. Molecular mechanisms controlling calcium entry through
AMPA-type glutamate receptor channels. Neuron. 1995;15(5):987-990. doi:10.1016/0896-6273(95)90087-X
apa: Jonas, P. M., & Burnashev, N. (1995). Molecular mechanisms controlling
calcium entry through AMPA-type glutamate receptor channels. Neuron. Elsevier.
https://doi.org/10.1016/0896-6273(95)90087-X
chicago: Jonas, Peter M, and Nail Burnashev. “Molecular Mechanisms Controlling Calcium
Entry through AMPA-Type Glutamate Receptor Channels.” Neuron. Elsevier,
1995. https://doi.org/10.1016/0896-6273(95)90087-X.
ieee: P. M. Jonas and N. Burnashev, “Molecular mechanisms controlling calcium entry
through AMPA-type glutamate receptor channels,” Neuron, vol. 15, no. 5.
Elsevier, pp. 987–990, 1995.
ista: Jonas PM, Burnashev N. 1995. Molecular mechanisms controlling calcium entry
through AMPA-type glutamate receptor channels. Neuron. 15(5), 987–990.
mla: Jonas, Peter M., and Nail Burnashev. “Molecular Mechanisms Controlling Calcium
Entry through AMPA-Type Glutamate Receptor Channels.” Neuron, vol. 15,
no. 5, Elsevier, 1995, pp. 987–90, doi:10.1016/0896-6273(95)90087-X.
short: P.M. Jonas, N. Burnashev, Neuron 15 (1995) 987–990.
date_created: 2018-12-11T12:03:27Z
date_published: 1995-11-01T00:00:00Z
date_updated: 2022-06-28T08:34:36Z
day: '01'
doi: 10.1016/0896-6273(95)90087-X
extern: '1'
external_id:
pmid:
- '7576666'
intvolume: ' 15'
issue: '5'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://www.sciencedirect.com/science/article/pii/089662739590087X?via%3Dihub
month: '11'
oa: 1
oa_version: Published Version
page: 987 - 990
pmid: 1
publication: Neuron
publication_identifier:
issn:
- 0896-6273
publication_status: published
publisher: Elsevier
publist_id: '2926'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Molecular mechanisms controlling calcium entry through AMPA-type glutamate
receptor channels
type: journal_article
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 15
year: '1995'
...
---
_id: '3478'
abstract:
- lang: eng
text: 1. Properties of dendritic glutamate receptor (GluR) channels were investigated
using fast application of glutamate to outside-out membrane patches isolated from
the apical dendrites of CA3 and CA1 pyramidal neurons in rat hippocampal slices.
CA3 patches were formed (15-76 μm from the soma) in the region of messy fibre
(MF) synapses, and CA1 patches (25-174 μm from the soma) in the region of Schaffer
collateral (SC) innervation. 2. Dual-component responses consisting of a rapidly
rising and decaying component followed by a second, substantially slower, component
were elicited by 1 ms pulses of 1 mM glutamate in the presence of 10 μM glycine
and absence of external Mg2+. The fast component was selectively blocked by 2-5
μM 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX) and the slow component by 30 μM
D-2-amino-5-phosphonopentanoic acid (D-AP5), suggesting that the fast and slow
components were mediated by the GluR channels of the L-α-amino-3-hydroxy-5-methyl-4-isoxazolepropionate
(AMPA) and NMDA type, respectively. The peak amplitude ratio of the NMDA to AMPA
receptor-mediated components varied between 0.03 and 0.62 in patches from both
CA3 and CA1 dendrites. Patches lacking either component were rarely observed.
3. The peak current-voltage (I-V) relationship of the fast component was almost
linear, whereas the I-V relationship of the slow component showed a region of
negative slope in the presence of 1 mM external Mg2+. The reversal potential for
both components was close to 0 mV. 4. Kainate-preferring GluR channels did not
contribute appreciably to the response to glutamate. The responses to 100 ms pulses
of 1 mM glutamate were mimicked by application of 1 mM AMPA, whereas 1 mM kainate
produced much smaller, weakly desensitizing currents. This suggests that the fast
component is primarily mediated by the action of glutamate on AMPA-preferring
receptors. 5. The mean elementary conductance of AMPA receptor channels was about
10 pS, as estimated by non-stationary fluctuation analysis. The permeability of
these channels to Ca2+ was low (~5% of the permeability to Cs+). 6. The elementary
conductance of NMDA receptor channels was larger, with a main conductance state
of about 45 pS. These channels were 3.6 times more permeable to Ca2+ than to Cs+.
7. AMPA receptor-mediated currents activated rapidly in response to 1 ms pulses
of 1 mM glutamate and deactivated with a predominant, fast time constant and a
smaller, slower component (τ1≃2 ms, τ2≃8 ms, contributing ~80 and ~20% to the
total decay amplitude, respectively). Desensitization of the current during a
100 ms pulse was best fitted by two time constants (τ1≃10 ms, ~60%; τ2≃34 ms,
~40%). 8. NMDA receptor-mediated currents in response to 1 ms pulses of 1 mM glutamate
activated and deactivated much more slowly than AMPA receptor-mediated currents.
The time course could be described by a single exponential rising phase (τ≃7 ms)
followed by a double exponential decay (τ1≃200 ms, ~80%; τ2≃1-3 s, ~20%). 9. Mg2+
blocked the NMDA component in a voltage-dependent manner, with a half-maximal
inhibitory concentration (IC50) of 21 μM at -80 mV. At physiological Mg2+ concentrations,
block of the NMDA component could be rapidly relieved with voltage jumps from
negative to positive potentials. Block of the current upon return to negative
potentials occurred almost instantaneously. 10. Zn2+ also selectively-blocked
the NMDA receptor-mediated current with an IC50 of 22 μM, but this block differed
from that of Mg2+ in that it showed little voltage dependence. Rapid application
of Zn2+ together with glutamate produced partial block of the current. More block
was observed if Zn2+ and glutamate were co-applied when NMDA receptor channels
were already open. 11. The functional properties of dendritic GluRs were similar
to those found at the soma. Knowledge of these properties facilitated simulations
investigating the contribution of coactivated AMPA and NMDA receptors to synaptic
depolarization and Ca2+ entry into dendritic spines. Because of its slow deactivation,
the NMDA receptor-mediated current contributes substantially to depolarization
and Ca2+ entry and is susceptible to modulation over a period of seconds, either
by backpropagating action potentials or by the release of Zn2+ from presynaptic
boutons.
acknowledgement: We thank M.Hausser, A.Roth, P.Ruppersberg, and G.Stuart for helpful
discussions and M.H. and G.S. for critically reading the manuscript. We also thank
M.Kaiser for expert technical assistance and F.Helmchen, M.Huke and A.Roth for computer
programming. Financial support from the Alexander von Humboldt Foundation and the
Deutsche Forschungsgemeinschaft (SFB317) is gratefully acknowledged.
article_processing_charge: No
article_type: original
author:
- first_name: Nelson
full_name: Spruston, Nelson
last_name: Spruston
- first_name: Peter M
full_name: Jonas, Peter M
id: 353C1B58-F248-11E8-B48F-1D18A9856A87
last_name: Jonas
orcid: 0000-0001-5001-4804
- first_name: Bert
full_name: Sakmann, Bert
last_name: Sakmann
citation:
ama: Spruston N, Jonas PM, Sakmann B. Dendritic glutamate receptor channels in rat
hippocampal CA3 and CA1 pyramidal neurons. Journal of Physiology. 1995;482(Pt
2):325-352. doi:10.1113/jphysiol.1995.sp020521
apa: Spruston, N., Jonas, P. M., & Sakmann, B. (1995). Dendritic glutamate receptor
channels in rat hippocampal CA3 and CA1 pyramidal neurons. Journal of Physiology.
Wiley-Blackwell. https://doi.org/10.1113/jphysiol.1995.sp020521
chicago: Spruston, Nelson, Peter M Jonas, and Bert Sakmann. “Dendritic Glutamate
Receptor Channels in Rat Hippocampal CA3 and CA1 Pyramidal Neurons.” Journal
of Physiology. Wiley-Blackwell, 1995. https://doi.org/10.1113/jphysiol.1995.sp020521.
ieee: N. Spruston, P. M. Jonas, and B. Sakmann, “Dendritic glutamate receptor channels
in rat hippocampal CA3 and CA1 pyramidal neurons,” Journal of Physiology,
vol. 482, no. Pt 2. Wiley-Blackwell, pp. 325–352, 1995.
ista: Spruston N, Jonas PM, Sakmann B. 1995. Dendritic glutamate receptor channels
in rat hippocampal CA3 and CA1 pyramidal neurons. Journal of Physiology. 482(Pt
2), 325–352.
mla: Spruston, Nelson, et al. “Dendritic Glutamate Receptor Channels in Rat Hippocampal
CA3 and CA1 Pyramidal Neurons.” Journal of Physiology, vol. 482, no. Pt
2, Wiley-Blackwell, 1995, pp. 325–52, doi:10.1113/jphysiol.1995.sp020521.
short: N. Spruston, P.M. Jonas, B. Sakmann, Journal of Physiology 482 (1995) 325–352.
date_created: 2018-12-11T12:03:32Z
date_published: 1995-01-15T00:00:00Z
date_updated: 2022-06-28T08:08:40Z
day: '15'
doi: 10.1113/jphysiol.1995.sp020521
extern: '1'
external_id:
pmid:
- '7536248'
intvolume: ' 482'
issue: Pt 2
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://physoc.onlinelibrary.wiley.com/doi/abs/10.1113/jphysiol.1995.sp020521
month: '01'
oa: 1
oa_version: Published Version
page: 325 - 352
pmid: 1
publication: Journal of Physiology
publication_identifier:
issn:
- 0022-3751
publication_status: published
publisher: Wiley-Blackwell
publist_id: '2909'
quality_controlled: '1'
status: public
title: Dendritic glutamate receptor channels in rat hippocampal CA3 and CA1 pyramidal
neurons
type: journal_article
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 482
year: '1995'
...
---
_id: '3479'
abstract:
- lang: eng
text: 1. Glutamate receptor (GluR) channels were studied in basket cells in the
dentate gyrus of rat hippocampal slices. Basket cells were identified by their
location, dendritic morphology and high frequency of action potentials generated
during sustained current injection. 2. Dual-component currents were activated
by fast application of glutamate to outside-out membrane patches isolated from
basket cell somata (10 μM glycine, no external Mg2+). The fast component was selectively
blocked by 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX), the slow component by
D-2-amino-5-phosphonopentanoic acid (D-AP5). This suggests that the two components
were mediated by α-amino-3-hydroxy-5-methyl-4-isoxazolepropionate receptor (AMPAR)/kainate
receptor and N-methyl-D-aspartate receptor (NMDAR) channels, respectively. The
mean ratio of the peak current of the NMDAR component to that of the AMPAR/kainate
receptor component was 0.22 (1 ms pulses of 10 mM glutamate). 3. The AMPAR/kainate
receptor component, which was studied in isolation in the presence of D-AP5, was
identified as AMPAR mediated on the basis of the preferential activation by AMPA
as compared with kainate, the weak desensitization of kainate-activated currents,
the cross-desensitization between AMPA and kainate, and the reduction of desensitization
by cyclothiazide. 4. Deactivation of basket cell AMPARs following 1 ms pulses
of glutamate occurred with a time constant (τ) of 1.2 ± 0.1 ms (mean ± S.E.M.).
During 100 ms glutamate pulses, AMPARs desensitized with a τ of 3.7 ± 0.2 ms.
5. The peak current-voltage (I-V) relation of AMPAR-mediated currents in Na+-rich
extracellular solution showed a reversal potential of -4.0 ± 2.6 mV and was characterized
by a doubly rectifying shape. The conductance of single AMPAR channels was estimated
as 22.6 ± 1.6 pS using non-stationary fluctuation analysis. AMPARs expressed in
hippocampal basket cells mere highly Ca2+ permeable (P(Ca)/P(K) = 1.79). 6. NMDARs
in hippocampal basket cells were studied in isolation in the presence of CNQX.
Deactivation of NMDARs activated by glutamate pulses occurred bi-exponentially
with mean τ values of 266 ± 23 ms (76%) and 2620 ± 383 ms (24%). 7. The peak I-V
relation of the NMDAR-mediated component in Na+-rich extracellular solution showed
a reversal potential of 1.5 ± 0.6 mV and a region of negative slope at negative
membrane potentials in the presence of external Mg2+, due to voltage-dependent
block by these ions. The conductance of single NMDAR channels in the main open
state was 50.2 ± 1.8 pS. NMDARs in hippocampal basket cells were highly permeable
to Ca2+ (P(Ca)/P(K) = 6.68). 8. AMPARs in hippocampal basket cells are characterized
by about threefold faster kinetics and twentyfold higher Ca2+ permeability than
AMPARs in hippocampal granule or pyramidal cells. Simulations show that the Ca2+
influx through basket cell AMPARs is comparable to that through NMDARs at negative
membrane potentials with physiological concentrations of Ca2+ and Mg2+. This suggests
a dual pathway of synaptically mediated Ca2+ entry into interneurones.
acknowledgement: We thank Drs M.Häusser and H.Markram for critically reading the manuscript
and M.Kaiser for technical assistance. Supported by the Deutsche Forschungsgemeinschaft
(SFB-317/B14 grant to P.J. and a Graduiertenkollegstipendium to J.R.P.G.)
article_processing_charge: No
article_type: original
author:
- first_name: Duk
full_name: Koh, Duk
last_name: Koh
- first_name: Jörg
full_name: Geiger, Jörg
last_name: Geiger
- first_name: Peter M
full_name: Jonas, Peter M
id: 353C1B58-F248-11E8-B48F-1D18A9856A87
last_name: Jonas
orcid: 0000-0001-5001-4804
- first_name: Bert
full_name: Sakmann, Bert
last_name: Sakmann
citation:
ama: Koh D, Geiger J, Jonas PM, Sakmann B. Ca(2+)-permeable AMPA and NMDA receptor
channels in basket cells of rat hippocampal dentate gyrus. Journal of Physiology.
1995;485(Pt 2):383-402. doi:10.1113/jphysiol.1995.sp020737
apa: Koh, D., Geiger, J., Jonas, P. M., & Sakmann, B. (1995). Ca(2+)-permeable
AMPA and NMDA receptor channels in basket cells of rat hippocampal dentate gyrus.
Journal of Physiology. Wiley-Blackwell. https://doi.org/10.1113/jphysiol.1995.sp020737
chicago: Koh, Duk, Jörg Geiger, Peter M Jonas, and Bert Sakmann. “Ca(2+)-Permeable
AMPA and NMDA Receptor Channels in Basket Cells of Rat Hippocampal Dentate Gyrus.”
Journal of Physiology. Wiley-Blackwell, 1995. https://doi.org/10.1113/jphysiol.1995.sp020737.
ieee: D. Koh, J. Geiger, P. M. Jonas, and B. Sakmann, “Ca(2+)-permeable AMPA and
NMDA receptor channels in basket cells of rat hippocampal dentate gyrus,” Journal
of Physiology, vol. 485, no. Pt 2. Wiley-Blackwell, pp. 383–402, 1995.
ista: Koh D, Geiger J, Jonas PM, Sakmann B. 1995. Ca(2+)-permeable AMPA and NMDA
receptor channels in basket cells of rat hippocampal dentate gyrus. Journal of
Physiology. 485(Pt 2), 383–402.
mla: Koh, Duk, et al. “Ca(2+)-Permeable AMPA and NMDA Receptor Channels in Basket
Cells of Rat Hippocampal Dentate Gyrus.” Journal of Physiology, vol. 485,
no. Pt 2, Wiley-Blackwell, 1995, pp. 383–402, doi:10.1113/jphysiol.1995.sp020737.
short: D. Koh, J. Geiger, P.M. Jonas, B. Sakmann, Journal of Physiology 485 (1995)
383–402.
date_created: 2018-12-11T12:03:33Z
date_published: 1995-06-01T00:00:00Z
date_updated: 2022-06-28T07:54:44Z
day: '01'
doi: 10.1113/jphysiol.1995.sp020737
extern: '1'
external_id:
pmid:
- '7545230'
intvolume: ' 485'
issue: Pt 2
language:
- iso: eng
main_file_link:
- open_access: '1'
url: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1158000/pdf/jphysiol00319-0104.pdf
month: '06'
oa: 1
oa_version: Published Version
page: 383 - 402
pmid: 1
publication: Journal of Physiology
publication_identifier:
issn:
- 0022-3751
publication_status: published
publisher: Wiley-Blackwell
publist_id: '2908'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Ca(2+)-permeable AMPA and NMDA receptor channels in basket cells of rat hippocampal
dentate gyrus
type: journal_article
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 485
year: '1995'
...
---
_id: '3480'
abstract:
- lang: eng
text: Recording of glutamate-activated currents in membrane patches was combine
with RT-PCR-mediated AMPA receptor (AMPAR) subunit mRNA analysis in single identified
cells of rat brain slices. Analysis of AMPARs in principal neurons end interneurons
of hippocampus and neocortex and in auditory relay neurons and Bergmann glial
cells indicates that the GluR-B subunit in its flip version determines formation
of receptors with relatively slow gating, whereas the GluR-D subunit promotes
assembly of more rapidly gated receptors. The relation between Ca 2+ permeability
of AMPAR channels and the relative GluR-B mRNA abundance is consistent with the
dominance of this subunit in determining the Ca 2+ permeability of native receptors.
The results suggest that differential expression of GluR-B and GluR-D subunit
genes, as well as splicing end editing of their mRNAs, account for the differences
in gating and Ca 2+ permeability of native AMPAR channels.
acknowledgement: "We thank Ulla Amtmann for efficient help with the molecular analysis.
We also thank M. Kaiser for technical assistance, Dr. J. G. G. Borst for advice
concerning preparation of brainstem slices, and Drs. N. Spruston and G. Stuart for
critically reading the manuscript. Funded in part by Bundesministerium für Forschung
und Technologie grant BCT 364 AZ 321/7291 (P. H. S.) and by Deutsche Forschungsgemeinschaftgrant
SFB-3171814(P. J.). J. R. P. G. and T. M. were supported by the graduate program
of Molecular and Cellular Neurobiology of the University of Heidelberg. The costs
of publication of this article were defrayed in part by the payment of page charges.
This article must therefore be hereby\r\nmarked “advertisement” in accordance with
18 USC Section 1734 solely to Indicate this fact."
article_processing_charge: No
article_type: original
author:
- first_name: Jörg
full_name: Geiger, Jörg
last_name: Geiger
- first_name: Thorsten
full_name: Melcher, Thorsten
last_name: Melcher
- first_name: Duk
full_name: Koh, Duk
last_name: Koh
- first_name: Bert
full_name: Sakmann, Bert
last_name: Sakmann
- first_name: Peter
full_name: Seeburg, Peter
last_name: Seeburg
- first_name: Peter M
full_name: Jonas, Peter M
id: 353C1B58-F248-11E8-B48F-1D18A9856A87
last_name: Jonas
orcid: 0000-0001-5001-4804
- first_name: Hannah
full_name: Monyer, Hannah
last_name: Monyer
citation:
ama: Geiger J, Melcher T, Koh D, et al. Relative abundance of subunit mRNAs determines
gating and Ca(2+) permeability of AMPA receptors in principal neurons and interneurons
in rat CNS. Neuron. 1995;15(1):193-204. doi:10.1016/0896-6273(95)90076-4
apa: Geiger, J., Melcher, T., Koh, D., Sakmann, B., Seeburg, P., Jonas, P. M., &
Monyer, H. (1995). Relative abundance of subunit mRNAs determines gating and Ca(2+)
permeability of AMPA receptors in principal neurons and interneurons in rat CNS.
Neuron. Elsevier. https://doi.org/10.1016/0896-6273(95)90076-4
chicago: Geiger, Jörg, Thorsten Melcher, Duk Koh, Bert Sakmann, Peter Seeburg, Peter
M Jonas, and Hannah Monyer. “Relative Abundance of Subunit MRNAs Determines Gating
and Ca(2+) Permeability of AMPA Receptors in Principal Neurons and Interneurons
in Rat CNS.” Neuron. Elsevier, 1995. https://doi.org/10.1016/0896-6273(95)90076-4.
ieee: J. Geiger et al., “Relative abundance of subunit mRNAs determines gating
and Ca(2+) permeability of AMPA receptors in principal neurons and interneurons
in rat CNS,” Neuron, vol. 15, no. 1. Elsevier, pp. 193–204, 1995.
ista: Geiger J, Melcher T, Koh D, Sakmann B, Seeburg P, Jonas PM, Monyer H. 1995.
Relative abundance of subunit mRNAs determines gating and Ca(2+) permeability
of AMPA receptors in principal neurons and interneurons in rat CNS. Neuron. 15(1),
193–204.
mla: Geiger, Jörg, et al. “Relative Abundance of Subunit MRNAs Determines Gating
and Ca(2+) Permeability of AMPA Receptors in Principal Neurons and Interneurons
in Rat CNS.” Neuron, vol. 15, no. 1, Elsevier, 1995, pp. 193–204, doi:10.1016/0896-6273(95)90076-4.
short: J. Geiger, T. Melcher, D. Koh, B. Sakmann, P. Seeburg, P.M. Jonas, H. Monyer,
Neuron 15 (1995) 193–204.
date_created: 2018-12-11T12:03:33Z
date_published: 1995-07-01T00:00:00Z
date_updated: 2022-06-28T07:47:09Z
day: '01'
doi: 10.1016/0896-6273(95)90076-4
extern: '1'
external_id:
pmid:
- '7619522'
intvolume: ' 15'
issue: '1'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://www.sciencedirect.com/science/article/pii/0896627395900764?via%3Dihub
month: '07'
oa: 1
oa_version: Published Version
page: 193 - 204
pmid: 1
publication: Neuron
publication_identifier:
issn:
- 0896-6273
publication_status: published
publisher: Elsevier
publist_id: '2907'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Relative abundance of subunit mRNAs determines gating and Ca(2+) permeability
of AMPA receptors in principal neurons and interneurons in rat CNS
type: journal_article
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 15
year: '1995'
...
---
_id: '3481'
abstract:
- lang: eng
text: 1. The influence of intracellular factors on current rectification of different
subtypes of native α-amino-3-hydroxy-5-methyl-4-isoxazolepropionate receptors
(AMPARs) was studied in rat brain slices by combining fast application of glutamate
with patch pipette perfusion. 2. The peak current-voltage (I-V) relation of the
AMPARs expressed in Bergmann glial cells of cerebellum and dentate gyrus (DG)
basket cells of hippocampus was weakly rectifying in outside-out patches and nystatin-perforated
vesicles, but showed a doubly rectifying shape with a region of reduced slope
between 0 and +40 mV in nucleated patches. The I-V relation of AMPARs expressed
in hippocampal CA3 pyramidal neurones was linear in all recording configurations.
3. Intracellular application of 2.5 μM spermine, a naturally occurring polyamine,
blocked outward currents in outside-oat patches from Bergmann glial cells and
DG basket cells in a voltage-dependent manner, generating I-V relations with a
doubly rectifying shape which were similar to those recorded in nucleated patches.
AMPARs in CA3 pyramidal cell patches were unaffected by 25 μM spermine. 4. The
half-maximal blocking concentration of spermine at +40 mV was 0.3 μM in Bergmann
glial cell patches and 1.5 μM in DG basket cell patches, whereas it was much higher
(≥ 100 μM) for CA3 pyramidal. cell patches. Spermidine also affected current rectification,
but with lower affinity. The block of outward current by polyamines following
voltage jumps developed within < 0.5 ms. 5. We conclude that current rectification,
rather than being an intrinsic property of the Ca2+ permeable AMPAR channel, is
generated by polyamine block.
acknowledgement: We thank Dr B.Sakmann, Dr V.Witzemann, J.Geiger, and A.Roth for helpful
discussions and Dr D.Feldmeyer and Dr A.Villarroel for reading the manuscript. We
also thank M.Kaiser for technical and H.Spiegel for secretarial assistance. Supported
by DFG grant SFB-317/B14(P.J.).
article_processing_charge: No
article_type: original
author:
- first_name: Duk
full_name: Koh, Duk
last_name: Koh
- first_name: Nail
full_name: Burnashev, Nail
last_name: Burnashev
- first_name: Peter M
full_name: Jonas, Peter M
id: 353C1B58-F248-11E8-B48F-1D18A9856A87
last_name: Jonas
orcid: 0000-0001-5001-4804
citation:
ama: Koh D, Burnashev N, Jonas PM. Block of native Ca(2+)-permeable AMPA receptors
in rat brain by intracellular polyamines generates double rectification. Journal
of Physiology. 1995;486(Pt 2):305-312. doi:10.1113/jphysiol.1995.sp020813
apa: Koh, D., Burnashev, N., & Jonas, P. M. (1995). Block of native Ca(2+)-permeable
AMPA receptors in rat brain by intracellular polyamines generates double rectification.
Journal of Physiology. Wiley-Blackwell. https://doi.org/10.1113/jphysiol.1995.sp020813
chicago: Koh, Duk, Nail Burnashev, and Peter M Jonas. “Block of Native Ca(2+)-Permeable
AMPA Receptors in Rat Brain by Intracellular Polyamines Generates Double Rectification.”
Journal of Physiology. Wiley-Blackwell, 1995. https://doi.org/10.1113/jphysiol.1995.sp020813.
ieee: D. Koh, N. Burnashev, and P. M. Jonas, “Block of native Ca(2+)-permeable AMPA
receptors in rat brain by intracellular polyamines generates double rectification,”
Journal of Physiology, vol. 486, no. Pt 2. Wiley-Blackwell, pp. 305–312,
1995.
ista: Koh D, Burnashev N, Jonas PM. 1995. Block of native Ca(2+)-permeable AMPA
receptors in rat brain by intracellular polyamines generates double rectification.
Journal of Physiology. 486(Pt 2), 305–312.
mla: Koh, Duk, et al. “Block of Native Ca(2+)-Permeable AMPA Receptors in Rat Brain
by Intracellular Polyamines Generates Double Rectification.” Journal of Physiology,
vol. 486, no. Pt 2, Wiley-Blackwell, 1995, pp. 305–12, doi:10.1113/jphysiol.1995.sp020813.
short: D. Koh, N. Burnashev, P.M. Jonas, Journal of Physiology 486 (1995) 305–312.
date_created: 2018-12-11T12:03:33Z
date_published: 1995-07-15T00:00:00Z
date_updated: 2022-06-27T14:53:16Z
day: '15'
doi: 10.1113/jphysiol.1995.sp020813
extern: '1'
external_id:
pmid:
- '7473198'
intvolume: ' 486'
issue: Pt 2
language:
- iso: eng
main_file_link:
- open_access: '1'
url: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1156754/
month: '07'
oa: 1
oa_version: Published Version
page: 305 - 312
pmid: 1
publication: Journal of Physiology
publication_identifier:
issn:
- 0022-3751
publication_status: published
publisher: Wiley-Blackwell
publist_id: '2906'
quality_controlled: '1'
status: public
title: Block of native Ca(2+)-permeable AMPA receptors in rat brain by intracellular
polyamines generates double rectification
type: journal_article
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 486
year: '1995'
...
---
_id: '3636'
abstract:
- lang: eng
text: 'Observations on the means, variances, and covariances of quantitative traits
across hybrid zones can give information similar to that from Mendelian markers.
In addition, they can identify particular traits through which the cline is maintained.
We describe a survey of six traits across the hybrid zone between Bombina bombina
and Bombina variegata (Amphibia: Discoglossidae) near Pescenica in Croatia. We
obtained laboratory measuments of the belly pattern, skin thickness, mating call,
skeletal form, egg size, and the developmental time of tadpoles. Although offspring
from hybrid populations showed no evidence of reduced viability, a third of the
F1 families failed completely, irrespective of the direction of the cross. All
traits differed significantly between the taxa. Clines in belly pattern, skin
thickness, mating call, and skeletal form were closely concordant with clines
in four diagnostic enzyme loci. However, the cline in developmental time was displaced
towards bombina, and the cline in egg size was displaced towards variegata. This
discordance could be because the traits are not inherited additively or because
they are subject to different selection pressures. We favor the latter explanation
in the case of developmental time. We show that moderate selection acting directly
on a trait suffices to shift its position; rather stronger selection is needed
to change its width appreciably. Within hybrid populations, there are significant
associations among quantitative traits, and between traits and enzymes. Phenotypic
variances also increase in hybrid populations. These observations can be explained
by linkage disequilibria among the underlying loci. However, the average magnitude
of the covariance between traits is about half that expected from the linkage
disequilibria between enzyme loci. The discrepancy is not readily explained by
nonadditive gene action. This puzzle is now unresolved and calls for further investigation.'
acknowledgement: 'The project would not have been possible without F. Perovic''s extensive
knowledge of the natural history of the Pegdenica area, and his assistance in the
field. Particular thanks are due to the Perovie family for their generous hospitality.
The Croatian Museum of Natural History and the Croatian Ministry of the Environment
were helpful in granting all the necessary permits. J. Szymura assisted with allozyme
tech-niques and in sharing unpublished data from his original survey of the area.
M. Davidson and K. Grant prepared the histological specimens, and G. Patterson volunteered
time and expertise in X-raying our toads. All members of L. Partridge''s lab generously
provided us with toad food on a daily basis, in the form of uncountably many spare
Drosophila. G. Malarky and M. Oh stoically coped with much tedious toad care. We
thank W. G. Hill, L. Kruuk, D. Rand, J. Szymura, and an anonymous reviewer for helpful
comments on the manuscript. This research was supported by a grant from the Natural
Environment Research Council (GR3/8002) to N.B. '
article_processing_charge: No
article_type: original
author:
- first_name: Beate
full_name: Nürnberger, Beate
last_name: Nürnberger
- first_name: Nicholas H
full_name: Barton, Nicholas H
id: 4880FE40-F248-11E8-B48F-1D18A9856A87
last_name: Barton
orcid: 0000-0002-8548-5240
- first_name: Catriona
full_name: Maccallum, Catriona
last_name: Maccallum
- first_name: Jason
full_name: Gilchrist, Jason
last_name: Gilchrist
- first_name: Michael
full_name: Appleby, Michael
last_name: Appleby
citation:
ama: Nürnberger B, Barton NH, Maccallum C, Gilchrist J, Appleby M. Natural selection
on quantitative traits in the Bombina hybrid zone. Evolution. 1995;49(6):1224-1238.
doi:10.1111/j.1558-5646.1995.tb04449.x
apa: Nürnberger, B., Barton, N. H., Maccallum, C., Gilchrist, J., & Appleby,
M. (1995). Natural selection on quantitative traits in the Bombina hybrid zone.
Evolution. Wiley-Blackwell. https://doi.org/10.1111/j.1558-5646.1995.tb04449.x
chicago: Nürnberger, Beate, Nicholas H Barton, Catriona Maccallum, Jason Gilchrist,
and Michael Appleby. “Natural Selection on Quantitative Traits in the Bombina
Hybrid Zone.” Evolution. Wiley-Blackwell, 1995. https://doi.org/10.1111/j.1558-5646.1995.tb04449.x.
ieee: B. Nürnberger, N. H. Barton, C. Maccallum, J. Gilchrist, and M. Appleby, “Natural
selection on quantitative traits in the Bombina hybrid zone,” Evolution,
vol. 49, no. 6. Wiley-Blackwell, pp. 1224–1238, 1995.
ista: Nürnberger B, Barton NH, Maccallum C, Gilchrist J, Appleby M. 1995. Natural
selection on quantitative traits in the Bombina hybrid zone. Evolution. 49(6),
1224–1238.
mla: Nürnberger, Beate, et al. “Natural Selection on Quantitative Traits in the
Bombina Hybrid Zone.” Evolution, vol. 49, no. 6, Wiley-Blackwell, 1995,
pp. 1224–38, doi:10.1111/j.1558-5646.1995.tb04449.x.
short: B. Nürnberger, N.H. Barton, C. Maccallum, J. Gilchrist, M. Appleby, Evolution
49 (1995) 1224–1238.
date_created: 2018-12-11T12:04:22Z
date_published: 1995-12-01T00:00:00Z
date_updated: 2022-06-27T12:58:02Z
day: '01'
doi: 10.1111/j.1558-5646.1995.tb04449.x
extern: '1'
intvolume: ' 49'
issue: '6'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1558-5646.1995.tb04449.x
month: '12'
oa: 1
oa_version: Published Version
page: 1224 - 1238
publication: Evolution
publication_identifier:
issn:
- 0014-3820
publication_status: published
publisher: Wiley-Blackwell
publist_id: '2747'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Natural selection on quantitative traits in the Bombina hybrid zone
type: journal_article
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 49
year: '1995'
...
---
_id: '3640'
abstract:
- lang: eng
text: 'The probability of fixation of a favorable mutation is reduced if selection
at other loci causes inherited variation in fitness. A general method for calculating
the fixation probability of an allele that can find itself in a variety of genetic
backgrounds is applied to find the effect of substitutions, fluctuating polymorphisms,
and deleterious mutations in a large population. With loose linkage, r, the effects
depend on the additive genetic variance in relative fitness, var(W), and act by
reducing effective population size by (N/Ne) = 1 + var(W)/2r2. However, tightly
linked loci can have a substantial effect not predictable from Ne. Linked deleterious
mutations reduce the fixation probability of weakly favored alleles by exp (-2U/R),
where U is the total mutation rate and R is the map length in Morgans. Substitutions
can cause a greater reduction: an allele with advantage s < scrit = (pi 2/6)
loge (S/s) [var(W)/R] is very unlikely to be fixed. (S is the advantage of the
substitution impeding fixation.) Fluctuating polymorphisms at many (n) linked
loci can also have a substantial effect, reducing fixation probability by exp
[square root of 2Kn var(W)/R] [K = -1/E((u-u)2/uv) depending on the frequencies
(u,v) at the selected polymorphisms]. Hitchhiking due to all three kinds of selection
may substantially impede adaptation that depends on weakly favored alleles.'
article_processing_charge: No
article_type: original
author:
- first_name: Nicholas H
full_name: Barton, Nicholas H
id: 4880FE40-F248-11E8-B48F-1D18A9856A87
last_name: Barton
orcid: 0000-0002-8548-5240
citation:
ama: Barton NH. Linkage and the limits to natural selection. Genetics. 1995;140(2):821-841.
doi:http://www.genetics.org/content/140/2/821.long
apa: Barton, N. H. (1995). Linkage and the limits to natural selection. Genetics.
Genetics Society of America. http://www.genetics.org/content/140/2/821.long
chicago: Barton, Nicholas H. “Linkage and the Limits to Natural Selection.” Genetics.
Genetics Society of America, 1995. http://www.genetics.org/content/140/2/821.long.
ieee: N. H. Barton, “Linkage and the limits to natural selection,” Genetics,
vol. 140, no. 2. Genetics Society of America, pp. 821–841, 1995.
ista: Barton NH. 1995. Linkage and the limits to natural selection. Genetics. 140(2),
821–841.
mla: Barton, Nicholas H. “Linkage and the Limits to Natural Selection.” Genetics,
vol. 140, no. 2, Genetics Society of America, 1995, pp. 821–41, doi:http://www.genetics.org/content/140/2/821.long.
short: N.H. Barton, Genetics 140 (1995) 821–841.
date_created: 2018-12-11T12:04:23Z
date_published: 1995-06-01T00:00:00Z
date_updated: 2022-06-24T09:59:08Z
day: '01'
doi: http://www.genetics.org/content/140/2/821.long
extern: '1'
external_id:
pmid:
- '7498757'
intvolume: ' 140'
issue: '2'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1206655/
month: '06'
oa: 1
oa_version: Published Version
page: 821 - 841
pmid: 1
publication: Genetics
publication_identifier:
issn:
- 0016-6731
publication_status: published
publisher: Genetics Society of America
publist_id: '2743'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Linkage and the limits to natural selection
type: journal_article
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 140
year: '1995'
...
---
_id: '4028'
abstract:
- lang: eng
text: Efficient algorithms are described for computing topological, combinatorial,
and metric properties of the union of finitely many spherical balls in R(d) These
algorithms are based on a simplicial complex dual to a decomposition of the union
of balls using Voronoi cells, and on short inclusion-exclusion formulas derived
from this complex. The algorithms are most relevant in R(3) where unions of finitely
many balls are commonly used as models of molecules.
acknowledgement: This work is supported by the National Science Foundation, under
Grant ASC-9200301, and the Alan T. Waterman award, Grant CCR-9118874. Any opinions,
findings, conclusions, or recommendations expressed in this publication are those
of the author and do not necessarily reflect the view of the National Science Foundation.
article_processing_charge: No
article_type: original
author:
- first_name: Herbert
full_name: Edelsbrunner, Herbert
id: 3FB178DA-F248-11E8-B48F-1D18A9856A87
last_name: Edelsbrunner
orcid: 0000-0002-9823-6833
citation:
ama: Edelsbrunner H. The union of balls and its dual shape. Discrete & Computational
Geometry. 1995;13(1):415-440. doi:10.1007/BF02574053
apa: Edelsbrunner, H. (1995). The union of balls and its dual shape. Discrete
& Computational Geometry. Springer. https://doi.org/10.1007/BF02574053
chicago: Edelsbrunner, Herbert. “The Union of Balls and Its Dual Shape.” Discrete
& Computational Geometry. Springer, 1995. https://doi.org/10.1007/BF02574053.
ieee: H. Edelsbrunner, “The union of balls and its dual shape,” Discrete &
Computational Geometry, vol. 13, no. 1. Springer, pp. 415–440, 1995.
ista: Edelsbrunner H. 1995. The union of balls and its dual shape. Discrete &
Computational Geometry. 13(1), 415–440.
mla: Edelsbrunner, Herbert. “The Union of Balls and Its Dual Shape.” Discrete
& Computational Geometry, vol. 13, no. 1, Springer, 1995, pp. 415–40,
doi:10.1007/BF02574053.
short: H. Edelsbrunner, Discrete & Computational Geometry 13 (1995) 415–440.
date_created: 2018-12-11T12:06:31Z
date_published: 1995-12-01T00:00:00Z
date_updated: 2022-06-27T08:14:48Z
day: '01'
doi: 10.1007/BF02574053
extern: '1'
intvolume: ' 13'
issue: '1'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://link.springer.com/article/10.1007/BF02574053
month: '12'
oa: 1
oa_version: Published Version
page: 415 - 440
publication: Discrete & Computational Geometry
publication_identifier:
issn:
- 0179-5376
publication_status: published
publisher: Springer
publist_id: '2095'
quality_controlled: '1'
scopus_import: '1'
status: public
title: The union of balls and its dual shape
type: journal_article
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 13
year: '1995'
...
---
_id: '4296'
abstract:
- lang: eng
text: Three replicate lines of Drosophila melanogaster were cultured at each of
two temperatures (16.5⚬C and 25⚬C) in population cages for 4 yr. The lifespans
of both sexes and the fecundity and fertility of the females were then measured
at both experimental temperatures. The characters showed evidence of adaptation;
flies of both sexes from each selection regime showed higher longevity, and females
showed higher fecundity and fertility, than flies from the other selection regime
when they were tested at the experimental temperature at which they had evolved.
Calculation of intrinsic rates of increase under different assumptions about the
rate of population increase showed that the difference between the lines from
the two selection regimes became less the higher the rate of population increase,
because the lines were more similar in early adulthood than they were later. Despite
the increased adaptation of the low-temperature lines to the low temperature,
like the high temperature lines they produced progeny at a higher rate at the
higher temperature. The lines may have independently evolved adaptations to their
respective thermal regimes during the experiment, or there may have been a trade-off
between adaptation to the two temperatures, or mutation pressure may have lowered
adaptation to the temperature that the flies no longer encountered.
acknowledgement: We thank Natural Environment Research Council and the Royal Society
for financial support.
article_processing_charge: No
article_type: original
author:
- first_name: Linda
full_name: Partridge, Linda
last_name: Partridge
- first_name: Brian
full_name: Barrie, Brian
last_name: Barrie
- first_name: Nicholas H
full_name: Barton, Nicholas H
id: 4880FE40-F248-11E8-B48F-1D18A9856A87
last_name: Barton
orcid: 0000-0002-8548-5240
- first_name: Kevin
full_name: Fowler, Kevin
last_name: Fowler
- first_name: Vernon
full_name: French, Vernon
last_name: French
citation:
ama: Partridge L, Barrie B, Barton NH, Fowler K, French V. Rapid laboratory evolution
of adult life history traits in Drosophila melanogaster in response to temperature.
Evolution. 1995;49(3):538-544. doi:10.1111/j.1558-5646.1995.tb02285.x
apa: Partridge, L., Barrie, B., Barton, N. H., Fowler, K., & French, V. (1995).
Rapid laboratory evolution of adult life history traits in Drosophila melanogaster
in response to temperature. Evolution. Wiley-Blackwell. https://doi.org/10.1111/j.1558-5646.1995.tb02285.x
chicago: Partridge, Linda, Brian Barrie, Nicholas H Barton, Kevin Fowler, and Vernon
French. “Rapid Laboratory Evolution of Adult Life History Traits in Drosophila
Melanogaster in Response to Temperature.” Evolution. Wiley-Blackwell, 1995.
https://doi.org/10.1111/j.1558-5646.1995.tb02285.x.
ieee: L. Partridge, B. Barrie, N. H. Barton, K. Fowler, and V. French, “Rapid laboratory
evolution of adult life history traits in Drosophila melanogaster in response
to temperature,” Evolution, vol. 49, no. 3. Wiley-Blackwell, pp. 538–544,
1995.
ista: Partridge L, Barrie B, Barton NH, Fowler K, French V. 1995. Rapid laboratory
evolution of adult life history traits in Drosophila melanogaster in response
to temperature. Evolution. 49(3), 538–544.
mla: Partridge, Linda, et al. “Rapid Laboratory Evolution of Adult Life History
Traits in Drosophila Melanogaster in Response to Temperature.” Evolution,
vol. 49, no. 3, Wiley-Blackwell, 1995, pp. 538–44, doi:10.1111/j.1558-5646.1995.tb02285.x.
short: L. Partridge, B. Barrie, N.H. Barton, K. Fowler, V. French, Evolution 49
(1995) 538–544.
date_created: 2018-12-11T12:08:06Z
date_published: 1995-06-01T00:00:00Z
date_updated: 2022-06-13T08:42:11Z
day: '01'
doi: 10.1111/j.1558-5646.1995.tb02285.x
extern: '1'
external_id:
pmid:
- '28565092 '
intvolume: ' 49'
issue: '3'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1558-5646.1995.tb02285.x
month: '06'
oa: 1
oa_version: Published Version
page: 538 - 544
pmid: 1
publication: Evolution
publication_identifier:
issn:
- 0014-3820
publication_status: published
publisher: Wiley-Blackwell
publist_id: '1778'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Rapid laboratory evolution of adult life history traits in Drosophila melanogaster
in response to temperature
type: journal_article
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 49
year: '1995'
...
---
_id: '4297'
abstract:
- lang: eng
text: The F5 (2n = 34) and FM2 (2n = 44-46) chromosome races of the Sceloporus grammicus
complex form a parapatric hybrid zone in the Mexican state of Hidalgo, characterized
by steep concordant clines among three diagnostic chromosome markers across a
straight-line distance of about 2 km. Here, we show that this zone is actually
structured into local patches in which hybridization extends over an extremely
irregular front. The distribution of hybrid-index (HI) scores across the transect
reveals some hybridization at almost all localities mapped in a central 7 km x
3 km area. Pooling the central samples produces both a strong heterozygote deficit
for all diagnostic markers and strong linkage disequilibria between all pairwise
combinations of these (unlinked) markers. Moreover, a highly significant association
exists between the habitat on which each individual was caught and its karyotype
(F5 chromosomes are more likely to be found on oak). Analysis of genotype frequencies
over a range of spatial scales shows that there is no significant heterozygote
deficit or habitat association within local areas of less than about 200 m; however,
there is significant linkage disequilibrium over the smallest scales (R = D (pquv)1/2
= 0.29, support limits, 0.18-0.36) over 100 m. These patterns suggest that lizards
mate and choose habitats randomly within local patches. This conclusion is supported
by mark-recapture estimates of dispersal (≈ 80 m in a generation) and by inference
of matings from embryo and maternal karyotypes. Closer examination of the two-dimensional
pattern reveals a convoluted cline for all three markers, with a width of 830
m (support limits 770 m-930 m). This cline width, combined with the strength of
local linkage disequilibrium, implies a dispersal rate of σ = 160 m in a generation
and an effective selection pressure of 30% on each chromosome marker. The proportion
of inviable embryos is greater in females from the center of the hybrid zone;
this is caused by effects associated with both karyotype and location. The hybrid
zone is likely to be maintained by selection against chromosomal heterozygotes,
by other kinds of selection against hybrids, and by selection adapting the chromosome
races to different habitats. The structure of the contact may be caused by both
random drift and by selection in relation to habitat.
acknowledgement: For field assistance in collecting and mapping of the zone, we thank
E. Arevalo, I. Goyenechea, D. Hutchison, M. Man- cilia, F. Mendoza, D. Mink, and
J. and H. Sites. The mark- recapture work was carried out by M. Mancilla,
F Mendoza, and A. Gonzales. J.W.S. also thanks T. Hinckley and D. Stevens of the Brigham Young University Department of
Geography for lessons in surveying and map making and use of the field equipment and planimeter. B. Nürnberger provided
the digitized coordinates for individual lizards and assisted with the analysis of
spatial structure and viability. B. Nürnberger, C. MacCallum, J. Mallet, and
J. Searle also provided helpful comments on the manuscript. This work was
supported by National Science Foundation grants BSR 85- 09092 and 88-22751 to
J.W.S., and grants from the Science and Engineering Research Council (GR/H09929)
and Natural Environment Research Council (GR3/8002) and the DarwinTrust to N.H.B.
The Mexican agency Secretaria de DesarrolloUrbano y Ecologia (now Secretaria de Desarrollo Social)
kindly provided scientific collecting permits (to E. Arévalo) for field work in 1989 and 1991.
article_processing_charge: No
article_type: original
author:
- first_name: Jack
full_name: Sites, Jack
last_name: Sites
- first_name: Nicholas H
full_name: Barton, Nicholas H
id: 4880FE40-F248-11E8-B48F-1D18A9856A87
last_name: Barton
orcid: 0000-0002-8548-5240
- first_name: Kent
full_name: Reed, Kent
last_name: Reed
citation:
ama: Sites J, Barton NH, Reed K. The genetic structure of a mosaic hybrid zone between
two chromosome races of the Sceloporus grammicus complex (Sauria, Phrynosomatidae)
in central Mexico. Evolution. 1995;49(1):9-36. doi:10.1111/j.1558-5646.1995.tb05955.x
apa: Sites, J., Barton, N. H., & Reed, K. (1995). The genetic structure of a
mosaic hybrid zone between two chromosome races of the Sceloporus grammicus complex
(Sauria, Phrynosomatidae) in central Mexico. Evolution. Wiley-Blackwell.
https://doi.org/10.1111/j.1558-5646.1995.tb05955.x
chicago: Sites, Jack, Nicholas H Barton, and Kent Reed. “The Genetic Structure of
a Mosaic Hybrid Zone between Two Chromosome Races of the Sceloporus Grammicus
Complex (Sauria, Phrynosomatidae) in Central Mexico.” Evolution. Wiley-Blackwell,
1995. https://doi.org/10.1111/j.1558-5646.1995.tb05955.x.
ieee: J. Sites, N. H. Barton, and K. Reed, “The genetic structure of a mosaic hybrid
zone between two chromosome races of the Sceloporus grammicus complex (Sauria,
Phrynosomatidae) in central Mexico,” Evolution, vol. 49, no. 1. Wiley-Blackwell,
pp. 9–36, 1995.
ista: Sites J, Barton NH, Reed K. 1995. The genetic structure of a mosaic hybrid
zone between two chromosome races of the Sceloporus grammicus complex (Sauria,
Phrynosomatidae) in central Mexico. Evolution. 49(1), 9–36.
mla: Sites, Jack, et al. “The Genetic Structure of a Mosaic Hybrid Zone between
Two Chromosome Races of the Sceloporus Grammicus Complex (Sauria, Phrynosomatidae)
in Central Mexico.” Evolution, vol. 49, no. 1, Wiley-Blackwell, 1995, pp.
9–36, doi:10.1111/j.1558-5646.1995.tb05955.x.
short: J. Sites, N.H. Barton, K. Reed, Evolution 49 (1995) 9–36.
date_created: 2018-12-11T12:08:06Z
date_published: 1995-02-01T00:00:00Z
date_updated: 2022-06-13T09:24:40Z
day: '01'
doi: 10.1111/j.1558-5646.1995.tb05955.x
extern: '1'
external_id:
pmid:
- '28593667'
intvolume: ' 49'
issue: '1'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1558-5646.1995.tb05955.x
month: '02'
oa: 1
oa_version: Published Version
page: 9 - 36
pmid: 1
publication: Evolution
publication_identifier:
issn:
- 0014-3820
publication_status: published
publisher: Wiley-Blackwell
publist_id: '1779'
quality_controlled: '1'
scopus_import: '1'
status: public
title: The genetic structure of a mosaic hybrid zone between two chromosome races
of the Sceloporus grammicus complex (Sauria, Phrynosomatidae) in central Mexico
type: journal_article
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 49
year: '1995'
...