---
_id: '14705'
abstract:
- lang: eng
text: Since the commercialization of brine shrimp (genus Artemia) in the 1950s,
this lineage, and in particular the model species Artemia franciscana, has been
the subject of extensive research. However, our understanding of the genetic mechanisms
underlying various aspects of their reproductive biology, including sex determination,
are still lacking. This is partly due to the scarcity of genomic resources for
Artemia species and crustaceans in general. Here, we present a chromosome-level
genome assembly of Artemia franciscana (Kellogg 1906), from the Great Salt Lake,
USA. The genome is 1GB, and the majority of the genome (81%) is scaffolded into
21 linkage groups using a previously published high-density linkage map. We performed
coverage and FST analyses using male and female genomic and transcriptomic reads
to quantify the extent of differentiation between the Z and W chromosomes. Additionally,
we quantified the expression levels in male and female heads and gonads and found
further evidence for dosage compensation in this species.
article_processing_charge: No
author:
- first_name: Marwan N
full_name: Elkrewi, Marwan N
id: 0B46FACA-A8E1-11E9-9BD3-79D1E5697425
last_name: Elkrewi
orcid: 0000-0002-5328-7231
citation:
ama: Elkrewi MN. Data from “Chromosome-level assembly of Artemia franciscana sheds
light on sex-chromosome differentiation.” 2024. doi:10.15479/AT:ISTA:14705
apa: Elkrewi, M. N. (2024). Data from “Chromosome-level assembly of Artemia franciscana
sheds light on sex-chromosome differentiation.” Institute of Science and Technology
Austria. https://doi.org/10.15479/AT:ISTA:14705
chicago: Elkrewi, Marwan N. “Data from ‘Chromosome-Level Assembly of Artemia Franciscana
Sheds Light on Sex-Chromosome Differentiation.’” Institute of Science and Technology
Austria, 2024. https://doi.org/10.15479/AT:ISTA:14705.
ieee: M. N. Elkrewi, “Data from ‘Chromosome-level assembly of Artemia franciscana
sheds light on sex-chromosome differentiation.’” Institute of Science and Technology
Austria, 2024.
ista: Elkrewi MN. 2024. Data from ‘Chromosome-level assembly of Artemia franciscana
sheds light on sex-chromosome differentiation’, Institute of Science and Technology
Austria, 10.15479/AT:ISTA:14705.
mla: Elkrewi, Marwan N. Data from “Chromosome-Level Assembly of Artemia Franciscana
Sheds Light on Sex-Chromosome Differentiation.” Institute of Science and Technology
Austria, 2024, doi:10.15479/AT:ISTA:14705.
short: M.N. Elkrewi, (2024).
contributor:
- contributor_type: researcher
first_name: Vincent K
id: 57854184-AAE0-11E9-8D04-98D6E5697425
last_name: Bett
- contributor_type: project_member
first_name: Ariana
id: 2A0848E2-F248-11E8-B48F-1D18A9856A87
last_name: Macon
- contributor_type: supervisor
first_name: Beatriz
id: 49E1C5C6-F248-11E8-B48F-1D18A9856A87
last_name: Vicoso
orcid: 0000-0002-4579-8306
- contributor_type: researcher
first_name: Marwan N
id: 0B46FACA-A8E1-11E9-9BD3-79D1E5697425
last_name: Elkrewi
orcid: 0000-0002-5328-7231
date_created: 2023-12-22T13:40:48Z
date_published: 2024-01-02T00:00:00Z
date_updated: 2025-07-24T11:06:43Z
day: '02'
ddc:
- '576'
department:
- _id: GradSch
- _id: BeVi
doi: 10.15479/AT:ISTA:14705
file:
- access_level: open_access
checksum: bdaf1392867786634ec5466d528c36ca
content_type: text/plain
creator: melkrewi
date_created: 2023-12-22T13:54:21Z
date_updated: 2023-12-22T13:54:21Z
file_id: '14707'
file_name: readme.txt.txt
file_size: 847
relation: main_file
success: 1
- access_level: open_access
checksum: 973e1cbdab923a71709782177980829f
content_type: application/x-zip-compressed
creator: melkrewi
date_created: 2023-12-22T14:14:06Z
date_updated: 2023-12-22T14:14:06Z
file_id: '14708'
file_name: data_artemia_franciscana_genome.zip
file_size: 343632753
relation: main_file
success: 1
file_date_updated: 2023-12-22T14:14:06Z
has_accepted_license: '1'
keyword:
- sex chromosome evolution
- genome assembly
- dosage compensation
license: https://creativecommons.org/licenses/by/4.0/
month: '01'
oa: 1
oa_version: Published Version
project:
- _id: 34ae1506-11ca-11ed-8bc3-c14f4c474396
grant_number: F8810
name: The highjacking of meiosis for asexual reproduction
publisher: Institute of Science and Technology Austria
related_material:
record:
- id: '15009'
relation: used_in_publication
status: public
retracted: '1'
status: public
title: Data from "Chromosome-level assembly of Artemia franciscana sheds light on
sex-chromosome differentiation"
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: research_data
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '2024'
...
---
_id: '14614'
abstract:
- lang: eng
text: 'Many insects carry an ancient X chromosome—the Drosophila Muller element
F—that likely predates their origin. Interestingly, the X has undergone turnover
in multiple fly species (Diptera) after being conserved for more than 450 My.
The long evolutionary distance between Diptera and other sequenced insect clades
makes it difficult to infer what could have contributed to this sudden increase
in rate of turnover. Here, we produce the first genome and transcriptome of scorpionflies
(genus Panorpa), an insect belonging to a long overlooked sister-order to Diptera:
Mecoptera. Combining our genome assembly with genomic short-read data, we obtain
genome coverage and identify X-linked super-scaffolds. We further perform a gene
homology analysis between the Panorpa X and a closely related Diptera species,
and we assess the conservation of the Panorpa X-linked gene content with that
of more distantly related insect species. We explored the structure of the Panorpa
X by determining its repeat content, GC content, and nucleotide diversity. Finally,
we used RNAseq data to detect the presence of dosage compensation in somatic tissues,
as well as to explore gene expression tissue-specificity, and sex-bias in gene
expression. We find high conservation of gene content between the mecopteran X
and the dipteran Muller F element, as well as several shared biological features,
such as the presence of dosage compensation and a low amount of genetic diversity,
consistent with a low recombination rate. However, the 2 homologous X chromosomes
differ strikingly in their size and number of genes they carry. Our results therefore
support a common ancestry of the mecopteran and ancestral dipteran X chromosomes,
and suggest that Muller element F shrank in size and gene content after the split
of Diptera and Mecoptera, which may have contributed to its turnover in dipteran
insects.'
article_processing_charge: No
author:
- first_name: Clementine
full_name: Lasne, Clementine
id: 02225f57-50d2-11eb-9ed8-8c92b9a34237
last_name: Lasne
orcid: 0000-0002-1197-8616
- first_name: Marwan N
full_name: Elkrewi, Marwan N
id: 0B46FACA-A8E1-11E9-9BD3-79D1E5697425
last_name: Elkrewi
orcid: 0000-0002-5328-7231
citation:
ama: Lasne C, Elkrewi MN. The scorpionfly (Panorpa cognata) genome highlights conserved
and derived features of the peculiar dipteran X chromosome. 2023. doi:10.15479/AT:ISTA:14614
apa: Lasne, C., & Elkrewi, M. N. (2023). The scorpionfly (Panorpa cognata) genome
highlights conserved and derived features of the peculiar dipteran X chromosome.
Institute of Science and Technology Austria. https://doi.org/10.15479/AT:ISTA:14614
chicago: Lasne, Clementine, and Marwan N Elkrewi. “The Scorpionfly (Panorpa Cognata)
Genome Highlights Conserved and Derived Features of the Peculiar Dipteran X Chromosome.”
Institute of Science and Technology Austria, 2023. https://doi.org/10.15479/AT:ISTA:14614.
ieee: C. Lasne and M. N. Elkrewi, “The scorpionfly (Panorpa cognata) genome highlights
conserved and derived features of the peculiar dipteran X chromosome.” Institute
of Science and Technology Austria, 2023.
ista: Lasne C, Elkrewi MN. 2023. The scorpionfly (Panorpa cognata) genome highlights
conserved and derived features of the peculiar dipteran X chromosome, Institute
of Science and Technology Austria, 10.15479/AT:ISTA:14614.
mla: Lasne, Clementine, and Marwan N. Elkrewi. The Scorpionfly (Panorpa Cognata)
Genome Highlights Conserved and Derived Features of the Peculiar Dipteran X Chromosome.
Institute of Science and Technology Austria, 2023, doi:10.15479/AT:ISTA:14614.
short: C. Lasne, M.N. Elkrewi, (2023).
contributor:
- contributor_type: researcher
first_name: Marwan N
id: 0B46FACA-A8E1-11E9-9BD3-79D1E5697425
last_name: Elkrewi
orcid: 0000-0002-5328-7231
date_created: 2023-11-27T16:39:19Z
date_published: 2023-12-01T00:00:00Z
date_updated: 2024-02-21T12:18:35Z
day: '01'
ddc:
- '576'
department:
- _id: BeVi
doi: 10.15479/AT:ISTA:14614
file:
- access_level: open_access
checksum: cd0f13322b5156819ecaebd2bc8e7d12
content_type: application/zip
creator: clasne
date_created: 2023-11-28T13:15:26Z
date_updated: 2023-11-28T13:15:26Z
file_id: '14625'
file_name: panorpaX.zip
file_size: 404968272
relation: main_file
success: 1
- access_level: open_access
checksum: 9ff600416577687a737cb3c96dfcb26c
content_type: text/plain
creator: clasne
date_created: 2023-11-30T14:16:59Z
date_updated: 2023-11-30T14:16:59Z
file_id: '14634'
file_name: panorpa_readme.txt
file_size: 2625
relation: main_file
success: 1
file_date_updated: 2023-11-30T14:16:59Z
has_accepted_license: '1'
keyword:
- Panorpa
- scorpionfly
- genome
- transcriptome
month: '12'
oa: 1
oa_version: Published Version
publisher: Institute of Science and Technology Austria
related_material:
record:
- id: '14613'
relation: used_in_publication
status: public
status: public
title: The scorpionfly (Panorpa cognata) genome highlights conserved and derived features
of the peculiar dipteran X chromosome
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: research_data
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '2023'
...