[{"year":"2003","external_id":{"pmid":["12705868"]},"keyword":["General Biochemistry","Genetics and Molecular Biology"],"status":"public","publication":"Cell","extern":"1","pmid":1,"date_published":"2003-04-17T00:00:00Z","doi":"10.1016/s0092-8674(03)00235-6","article_processing_charge":"No","publisher":"Elsevier","date_updated":"2022-07-18T08:57:42Z","_id":"11122","type":"journal_article","page":"195-206","quality_controlled":"1","month":"04","language":[{"iso":"eng"}],"issue":"2","citation":{"ista":"Walther TC, Alves A, Pickersgill H, Loı̈odice I, Hetzer M, Galy V, Hülsmann BB, Köcher T, Wilm M, Allen T, Mattaj IW, Doye V. 2003. The conserved Nup107-160 complex is critical for nuclear pore complex assembly. Cell. 113(2), 195–206.","chicago":"Walther, Tobias C., Annabelle Alves, Helen Pickersgill, Isabelle Loı̈odice, Martin Hetzer, Vincent Galy, Bastian B. Hülsmann, et al. “The Conserved Nup107-160 Complex Is Critical for Nuclear Pore Complex Assembly.” <i>Cell</i>. Elsevier, 2003. <a href=\"https://doi.org/10.1016/s0092-8674(03)00235-6\">https://doi.org/10.1016/s0092-8674(03)00235-6</a>.","mla":"Walther, Tobias C., et al. “The Conserved Nup107-160 Complex Is Critical for Nuclear Pore Complex Assembly.” <i>Cell</i>, vol. 113, no. 2, Elsevier, 2003, pp. 195–206, doi:<a href=\"https://doi.org/10.1016/s0092-8674(03)00235-6\">10.1016/s0092-8674(03)00235-6</a>.","apa":"Walther, T. C., Alves, A., Pickersgill, H., Loı̈odice, I., Hetzer, M., Galy, V., … Doye, V. (2003). The conserved Nup107-160 complex is critical for nuclear pore complex assembly. <i>Cell</i>. Elsevier. <a href=\"https://doi.org/10.1016/s0092-8674(03)00235-6\">https://doi.org/10.1016/s0092-8674(03)00235-6</a>","ama":"Walther TC, Alves A, Pickersgill H, et al. The conserved Nup107-160 complex is critical for nuclear pore complex assembly. <i>Cell</i>. 2003;113(2):195-206. doi:<a href=\"https://doi.org/10.1016/s0092-8674(03)00235-6\">10.1016/s0092-8674(03)00235-6</a>","short":"T.C. Walther, A. Alves, H. Pickersgill, I. Loı̈odice, M. Hetzer, V. Galy, B.B. Hülsmann, T. Köcher, M. Wilm, T. Allen, I.W. Mattaj, V. Doye, Cell 113 (2003) 195–206.","ieee":"T. C. Walther <i>et al.</i>, “The conserved Nup107-160 complex is critical for nuclear pore complex assembly,” <i>Cell</i>, vol. 113, no. 2. Elsevier, pp. 195–206, 2003."},"user_id":"72615eeb-f1f3-11ec-aa25-d4573ddc34fd","author":[{"first_name":"Tobias C.","last_name":"Walther","full_name":"Walther, Tobias C."},{"first_name":"Annabelle","last_name":"Alves","full_name":"Alves, Annabelle"},{"full_name":"Pickersgill, Helen","last_name":"Pickersgill","first_name":"Helen"},{"last_name":"Loı̈odice","full_name":"Loı̈odice, Isabelle","first_name":"Isabelle"},{"orcid":"0000-0002-2111-992X","first_name":"Martin W","last_name":"HETZER","id":"86c0d31b-b4eb-11ec-ac5a-eae7b2e135ed","full_name":"HETZER, Martin W"},{"last_name":"Galy","full_name":"Galy, Vincent","first_name":"Vincent"},{"first_name":"Bastian B.","last_name":"Hülsmann","full_name":"Hülsmann, Bastian B."},{"full_name":"Köcher, Thomas","last_name":"Köcher","first_name":"Thomas"},{"last_name":"Wilm","full_name":"Wilm, Matthias","first_name":"Matthias"},{"full_name":"Allen, Terry","last_name":"Allen","first_name":"Terry"},{"last_name":"Mattaj","full_name":"Mattaj, Iain W.","first_name":"Iain W."},{"first_name":"Valérie","full_name":"Doye, Valérie","last_name":"Doye"}],"day":"17","scopus_import":"1","title":"The conserved Nup107-160 complex is critical for nuclear pore complex assembly","oa_version":"Published Version","volume":113,"article_type":"original","date_created":"2022-04-07T07:57:10Z","intvolume":"       113","abstract":[{"text":"Nuclear pore complexes (NPCs) are large multiprotein assemblies that allow traffic between the cytoplasm and the nucleus. During mitosis in higher eukaryotes, the Nuclear Envelope (NE) breaks down and NPCs disassemble. How NPCs reassemble and incorporate into the NE upon mitotic exit is poorly understood. We demonstrate a function for the conserved Nup107-160 complex in this process. Partial in vivo depletion of Nup133 or Nup107 via RNAi in HeLa cells resulted in reduced levels of multiple nucleoporins and decreased NPC density in the NE. Immunodepletion of the entire Nup107-160 complex from in vitro nuclear assembly reactions produced nuclei with a continuous NE but no NPCs. This phenotype was reversible only if Nup107-160 complex was readded before closed NE formation. Depletion also prevented association of FG-repeat nucleoporins with chromatin. We propose a stepwise model in which postmitotic NPC assembly initiates on chromatin via early recruitment of the Nup107-160 complex.","lang":"eng"}],"publication_status":"published","publication_identifier":{"issn":["0092-8674"]}},{"oa":1,"language":[{"iso":"eng"}],"citation":{"ista":"Bilbao-Cortés D, Hetzer M, Längst G, Becker PB, Mattaj IW. 2002. Ran binds to chromatin by two distinct mechanisms. Current Biology. 12(13), 1151–1156.","chicago":"Bilbao-Cortés, Daniel, Martin Hetzer, Gernot Längst, Peter B. Becker, and Iain W. Mattaj. “Ran Binds to Chromatin by Two Distinct Mechanisms.” <i>Current Biology</i>. Elsevier BV, 2002. <a href=\"https://doi.org/10.1016/s0960-9822(02)00927-2\">https://doi.org/10.1016/s0960-9822(02)00927-2</a>.","apa":"Bilbao-Cortés, D., Hetzer, M., Längst, G., Becker, P. B., &#38; Mattaj, I. W. (2002). Ran binds to chromatin by two distinct mechanisms. <i>Current Biology</i>. Elsevier BV. <a href=\"https://doi.org/10.1016/s0960-9822(02)00927-2\">https://doi.org/10.1016/s0960-9822(02)00927-2</a>","mla":"Bilbao-Cortés, Daniel, et al. “Ran Binds to Chromatin by Two Distinct Mechanisms.” <i>Current Biology</i>, vol. 12, no. 13, Elsevier BV, 2002, pp. 1151–56, doi:<a href=\"https://doi.org/10.1016/s0960-9822(02)00927-2\">10.1016/s0960-9822(02)00927-2</a>.","ama":"Bilbao-Cortés D, Hetzer M, Längst G, Becker PB, Mattaj IW. Ran binds to chromatin by two distinct mechanisms. <i>Current Biology</i>. 2002;12(13):1151-1156. doi:<a href=\"https://doi.org/10.1016/s0960-9822(02)00927-2\">10.1016/s0960-9822(02)00927-2</a>","ieee":"D. Bilbao-Cortés, M. Hetzer, G. Längst, P. B. Becker, and I. W. Mattaj, “Ran binds to chromatin by two distinct mechanisms,” <i>Current Biology</i>, vol. 12, no. 13. Elsevier BV, pp. 1151–1156, 2002.","short":"D. Bilbao-Cortés, M. Hetzer, G. Längst, P.B. Becker, I.W. Mattaj, Current Biology 12 (2002) 1151–1156."},"issue":"13","user_id":"72615eeb-f1f3-11ec-aa25-d4573ddc34fd","month":"07","abstract":[{"text":"Ran GTPase plays important roles in nucleocytoplasmic transport in interphase [1, 2] and in both spindle formation and nuclear envelope (NE) assembly during mitosis [3, 4, 5]. The latter functions rely on the presence of high local concentrations of GTP-bound Ran near mitotic chromatin [3, 4, 5]. RanGTP localization has been proposed to result from the association of Ran's GDP/GTP exchange factor, RCC1, with chromatin [6, 7, 8, 9], but Ran is shown here to bind directly to chromatin in two modes, either dependent or independent of RCC1, and, where bound, to increase the affinity of chromatin for NE membranes. We propose that the Ran binding capacity of chromatin contributes to localized spindle and NE assembly.","lang":"eng"}],"intvolume":"        12","publication_identifier":{"issn":["0960-9822"]},"publication_status":"published","day":"09","scopus_import":"1","author":[{"first_name":"Daniel","last_name":"Bilbao-Cortés","full_name":"Bilbao-Cortés, Daniel"},{"orcid":"0000-0002-2111-992X","first_name":"Martin W","id":"86c0d31b-b4eb-11ec-ac5a-eae7b2e135ed","full_name":"HETZER, Martin W","last_name":"HETZER"},{"first_name":"Gernot","full_name":"Längst, Gernot","last_name":"Längst"},{"full_name":"Becker, Peter B.","last_name":"Becker","first_name":"Peter B."},{"first_name":"Iain W.","last_name":"Mattaj","full_name":"Mattaj, Iain W."}],"oa_version":"Published Version","title":"Ran binds to chromatin by two distinct mechanisms","volume":12,"date_created":"2022-04-07T07:57:31Z","article_type":"letter_note","publication":"Current Biology","status":"public","extern":"1","pmid":1,"date_published":"2002-07-09T00:00:00Z","year":"2002","external_id":{"pmid":["12121625"]},"keyword":["General Agricultural and Biological Sciences","General Biochemistry","Genetics and Molecular Biology"],"page":"1151-1156","main_file_link":[{"url":"https://doi.org/10.1016/S0960-9822(02)00927-2","open_access":"1"}],"quality_controlled":"1","article_processing_charge":"No","doi":"10.1016/s0960-9822(02)00927-2","publisher":"Elsevier BV","_id":"11124","date_updated":"2022-07-18T08:58:05Z","type":"journal_article"}]