[{"issue":"6","publication":"Chimia","author":[{"full_name":"Gong, Xiaochun","first_name":"Xiaochun","last_name":"Gong"},{"full_name":"Jordan, Inga","first_name":"Inga","last_name":"Jordan"},{"full_name":"Huppert, Martin","first_name":"Martin","last_name":"Huppert"},{"first_name":"Saijoscha","full_name":"Heck, Saijoscha","last_name":"Heck"},{"last_name":"Baykusheva","first_name":"Denitsa Rangelova","full_name":"Baykusheva, Denitsa Rangelova","id":"71b4d059-2a03-11ee-914d-dfa3beed6530"},{"first_name":"Denis","full_name":"Jelovina, Denis","last_name":"Jelovina"},{"last_name":"Schild","full_name":"Schild, Axel","first_name":"Axel"},{"full_name":"Wörner, Hans Jakob","first_name":"Hans Jakob","last_name":"Wörner"}],"oa_version":"Published Version","date_published":"2022-06-29T00:00:00Z","language":[{"iso":"eng"}],"type":"journal_article","doi":"10.2533/chimia.2022.520","publisher":"Swiss Chemical Society","publication_identifier":{"eissn":["2673-2424"],"issn":["0009-4293"]},"scopus_import":"1","main_file_link":[{"url":"https://doi.org/10.2533/chimia.2022.520","open_access":"1"}],"article_processing_charge":"No","volume":76,"page":"520-528","quality_controlled":"1","year":"2022","date_created":"2023-08-09T13:08:15Z","date_updated":"2023-08-22T07:26:39Z","title":"Attosecond photoionization dynamics: from molecules over clusters to the liquid phase","oa":1,"publication_status":"published","article_type":"original","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","month":"06","_id":"13993","keyword":["General Medicine","General Chemistry"],"intvolume":"        76","citation":{"short":"X. Gong, I. Jordan, M. Huppert, S. Heck, D.R. Baykusheva, D. Jelovina, A. Schild, H.J. Wörner, Chimia 76 (2022) 520–528.","ama":"Gong X, Jordan I, Huppert M, et al. Attosecond photoionization dynamics: from molecules over clusters to the liquid phase. <i>Chimia</i>. 2022;76(6):520-528. doi:<a href=\"https://doi.org/10.2533/chimia.2022.520\">10.2533/chimia.2022.520</a>","apa":"Gong, X., Jordan, I., Huppert, M., Heck, S., Baykusheva, D. R., Jelovina, D., … Wörner, H. J. (2022). Attosecond photoionization dynamics: from molecules over clusters to the liquid phase. <i>Chimia</i>. Swiss Chemical Society. <a href=\"https://doi.org/10.2533/chimia.2022.520\">https://doi.org/10.2533/chimia.2022.520</a>","chicago":"Gong, Xiaochun, Inga Jordan, Martin Huppert, Saijoscha Heck, Denitsa Rangelova Baykusheva, Denis Jelovina, Axel Schild, and Hans Jakob Wörner. “Attosecond Photoionization Dynamics: From Molecules over Clusters to the Liquid Phase.” <i>Chimia</i>. Swiss Chemical Society, 2022. <a href=\"https://doi.org/10.2533/chimia.2022.520\">https://doi.org/10.2533/chimia.2022.520</a>.","ista":"Gong X, Jordan I, Huppert M, Heck S, Baykusheva DR, Jelovina D, Schild A, Wörner HJ. 2022. Attosecond photoionization dynamics: from molecules over clusters to the liquid phase. Chimia. 76(6), 520–528.","ieee":"X. Gong <i>et al.</i>, “Attosecond photoionization dynamics: from molecules over clusters to the liquid phase,” <i>Chimia</i>, vol. 76, no. 6. Swiss Chemical Society, pp. 520–528, 2022.","mla":"Gong, Xiaochun, et al. “Attosecond Photoionization Dynamics: From Molecules over Clusters to the Liquid Phase.” <i>Chimia</i>, vol. 76, no. 6, Swiss Chemical Society, 2022, pp. 520–28, doi:<a href=\"https://doi.org/10.2533/chimia.2022.520\">10.2533/chimia.2022.520</a>."},"day":"29","status":"public","abstract":[{"text":"Photoionization is a process taking place on attosecond time scales. How its properties evolve from isolated particles to the condensed phase is an open question of both fundamental and practical relevance. Here, we review recent work that has advanced the study of photoionization dynamics from atoms to molecules, clusters and the liquid phase. The first measurements of molecular photoionization delays have revealed the attosecond dynamics of electron emission from a molecular shape resonance and their sensitivity to the molecular potential. Using electron-ion coincidence spectroscopy these measurements have been extended from isolated molecules to clusters. A continuous increase of the delays with the water-cluster size has been observed up to a size of 4-5 molecules, followed by a saturation towards larger clusters. Comparison with calculations has revealed a correlation of the time delay with the spatial extension of the created electron hole. Using cylindrical liquid-microjet techniques, these measurements have also been extended to liquid water, revealing a delay relative to isolated water molecules that was very similar to the largest water clusters studied. Detailed modeling based on Monte-Carlo simulations confirmed that these delays are dominated by the contributions of the first two solvation shells, which agrees with the results of the cluster measurements. These combined results open the perspective of experimentally characterizing the delocalization of electronic wave functions in complex systems and studying their evolution on attosecond time scales.","lang":"eng"}],"extern":"1"},{"has_accepted_license":"1","day":"12","intvolume":"        13","citation":{"apa":"Radler, P., Baranova, N. S., Dos Santos Caldas, P. R., Sommer, C. M., Lopez Pelegrin, M. D., Michalik, D., &#38; Loose, M. (2022). In vitro reconstitution of Escherichia coli divisome activation. <i>Nature Communications</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41467-022-30301-y\">https://doi.org/10.1038/s41467-022-30301-y</a>","chicago":"Radler, Philipp, Natalia S. Baranova, Paulo R Dos Santos Caldas, Christoph M Sommer, Maria D Lopez Pelegrin, David Michalik, and Martin Loose. “In Vitro Reconstitution of Escherichia Coli Divisome Activation.” <i>Nature Communications</i>. Springer Nature, 2022. <a href=\"https://doi.org/10.1038/s41467-022-30301-y\">https://doi.org/10.1038/s41467-022-30301-y</a>.","ista":"Radler P, Baranova NS, Dos Santos Caldas PR, Sommer CM, Lopez Pelegrin MD, Michalik D, Loose M. 2022. In vitro reconstitution of Escherichia coli divisome activation. Nature Communications. 13, 2635.","mla":"Radler, Philipp, et al. “In Vitro Reconstitution of Escherichia Coli Divisome Activation.” <i>Nature Communications</i>, vol. 13, 2635, Springer Nature, 2022, doi:<a href=\"https://doi.org/10.1038/s41467-022-30301-y\">10.1038/s41467-022-30301-y</a>.","ieee":"P. Radler <i>et al.</i>, “In vitro reconstitution of Escherichia coli divisome activation,” <i>Nature Communications</i>, vol. 13. Springer Nature, 2022.","short":"P. Radler, N.S. Baranova, P.R. Dos Santos Caldas, C.M. Sommer, M.D. Lopez Pelegrin, D. Michalik, M. Loose, Nature Communications 13 (2022).","ama":"Radler P, Baranova NS, Dos Santos Caldas PR, et al. In vitro reconstitution of Escherichia coli divisome activation. <i>Nature Communications</i>. 2022;13. doi:<a href=\"https://doi.org/10.1038/s41467-022-30301-y\">10.1038/s41467-022-30301-y</a>"},"keyword":["General Physics and Astronomy","General Biochemistry","Genetics and Molecular Biology","General Chemistry"],"ec_funded":1,"abstract":[{"text":"The actin-homologue FtsA is essential for E. coli cell division, as it links FtsZ filaments in the Z-ring to transmembrane proteins. FtsA is thought to initiate cell constriction by switching from an inactive polymeric to an active monomeric conformation, which recruits downstream proteins and stabilizes the Z-ring. However, direct biochemical evidence for this mechanism is missing. Here, we use reconstitution experiments and quantitative fluorescence microscopy to study divisome activation in vitro. By comparing wild-type FtsA with FtsA R286W, we find that this hyperactive mutant outperforms FtsA WT in replicating FtsZ treadmilling dynamics, FtsZ filament stabilization and recruitment of FtsN. We could attribute these differences to a faster exchange and denser packing of FtsA R286W below FtsZ filaments. Using FRET microscopy, we also find that FtsN binding promotes FtsA self-interaction. We propose that in the active divisome FtsA and FtsN exist as a dynamic copolymer that follows treadmilling filaments of FtsZ.","lang":"eng"}],"article_number":"2635","status":"public","department":[{"_id":"MaLo"}],"acknowledgement":"We acknowledge members of the Loose laboratory at IST Austria for helpful discussions—in particular L. Lindorfer for his assistance with cloning and purifications. We thank J. Löwe and T. Nierhaus (MRC-LMB Cambridge, UK) for sharing unpublished work and helpful discussions, as well as D. Vavylonis and D. Rutkowski (Lehigh University, Bethlehem, PA, USA) and S. Martin (University of Lausanne, Switzerland) for sharing their code for FRAP analysis. We are also thankful for the support by the Scientific Service Units (SSU) of IST Austria through resources provided by the Imaging and Optics Facility (IOF) and the Lab Support Facility (LSF). This work was supported by the European Research Council through grant ERC 2015-StG-679239 and by the Austrian Science Fund (FWF) StandAlone P34607 to M.L. and HFSP LT 000824/2016-L4 to N.B. For the purpose of open access, we have applied a CC BY public copyright licence to any Author Accepted Manuscript version arising from this submission.","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","month":"05","_id":"11373","article_type":"original","title":"In vitro reconstitution of Escherichia coli divisome activation","oa":1,"related_material":{"link":[{"url":"https://doi.org/10.1038/s41467-022-34485-1","relation":"erratum"}],"record":[{"status":"public","relation":"dissertation_contains","id":"14280"},{"status":"public","relation":"research_data","id":"10934"}]},"publication_status":"published","article_processing_charge":"No","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"scopus_import":"1","publication_identifier":{"issn":["2041-1723"]},"date_updated":"2024-02-21T12:35:18Z","project":[{"call_identifier":"H2020","_id":"2595697A-B435-11E9-9278-68D0E5697425","grant_number":"679239","name":"Self-Organization of the Bacterial Cell"},{"_id":"fc38323b-9c52-11eb-aca3-ff8afb4a011d","name":"Understanding bacterial cell division by in vitro\r\nreconstitution","grant_number":"P34607"}],"acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"}],"quality_controlled":"1","year":"2022","date_created":"2022-05-13T09:06:28Z","volume":13,"isi":1,"file":[{"file_name":"2022_NatureCommunications_Radler.pdf","date_created":"2022-05-13T09:10:51Z","file_id":"11374","content_type":"application/pdf","success":1,"access_level":"open_access","date_updated":"2022-05-13T09:10:51Z","relation":"main_file","checksum":"5af863ee1b95a0710f6ee864d68dc7a6","file_size":6945191,"creator":"dernst"}],"date_published":"2022-05-12T00:00:00Z","oa_version":"Published Version","external_id":{"isi":["000795171100037"]},"ddc":["570"],"author":[{"id":"40136C2A-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-9198-2182 ","last_name":"Radler","first_name":"Philipp","full_name":"Radler, Philipp"},{"full_name":"Baranova, Natalia S.","first_name":"Natalia S.","last_name":"Baranova","id":"38661662-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-3086-9124"},{"id":"38FCDB4C-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-6730-4461","last_name":"Dos Santos Caldas","first_name":"Paulo R","full_name":"Dos Santos Caldas, Paulo R"},{"full_name":"Sommer, Christoph M","first_name":"Christoph M","last_name":"Sommer","orcid":"0000-0003-1216-9105","id":"4DF26D8C-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Lopez Pelegrin, Maria D","first_name":"Maria D","last_name":"Lopez Pelegrin","id":"319AA9CE-F248-11E8-B48F-1D18A9856A87"},{"id":"B9577E20-AA38-11E9-AC9A-0930E6697425","first_name":"David","full_name":"Michalik, David","last_name":"Michalik"},{"orcid":"0000-0001-7309-9724","id":"462D4284-F248-11E8-B48F-1D18A9856A87","first_name":"Martin","full_name":"Loose, Martin","last_name":"Loose"}],"publication":"Nature Communications","publisher":"Springer Nature","doi":"10.1038/s41467-022-30301-y","file_date_updated":"2022-05-13T09:10:51Z","type":"journal_article","language":[{"iso":"eng"}]},{"keyword":["General Physics and Astronomy","General Biochemistry","Genetics and Molecular Biology","General Chemistry","Multidisciplinary"],"intvolume":"        13","citation":{"ama":"Ben Simon Y, Käfer K, Velicky P, Csicsvari JL, Danzl JG, Jonas PM. A direct excitatory projection from entorhinal layer 6b neurons to the hippocampus contributes to spatial coding and memory. <i>Nature Communications</i>. 2022;13. doi:<a href=\"https://doi.org/10.1038/s41467-022-32559-8\">10.1038/s41467-022-32559-8</a>","short":"Y. Ben Simon, K. Käfer, P. Velicky, J.L. Csicsvari, J.G. Danzl, P.M. Jonas, Nature Communications 13 (2022).","ieee":"Y. Ben Simon, K. Käfer, P. Velicky, J. L. Csicsvari, J. G. Danzl, and P. M. Jonas, “A direct excitatory projection from entorhinal layer 6b neurons to the hippocampus contributes to spatial coding and memory,” <i>Nature Communications</i>, vol. 13. Springer Nature, 2022.","mla":"Ben Simon, Yoav, et al. “A Direct Excitatory Projection from Entorhinal Layer 6b Neurons to the Hippocampus Contributes to Spatial Coding and Memory.” <i>Nature Communications</i>, vol. 13, 4826, Springer Nature, 2022, doi:<a href=\"https://doi.org/10.1038/s41467-022-32559-8\">10.1038/s41467-022-32559-8</a>.","chicago":"Ben Simon, Yoav, Karola Käfer, Philipp Velicky, Jozsef L Csicsvari, Johann G Danzl, and Peter M Jonas. “A Direct Excitatory Projection from Entorhinal Layer 6b Neurons to the Hippocampus Contributes to Spatial Coding and Memory.” <i>Nature Communications</i>. Springer Nature, 2022. <a href=\"https://doi.org/10.1038/s41467-022-32559-8\">https://doi.org/10.1038/s41467-022-32559-8</a>.","apa":"Ben Simon, Y., Käfer, K., Velicky, P., Csicsvari, J. L., Danzl, J. G., &#38; Jonas, P. M. (2022). A direct excitatory projection from entorhinal layer 6b neurons to the hippocampus contributes to spatial coding and memory. <i>Nature Communications</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41467-022-32559-8\">https://doi.org/10.1038/s41467-022-32559-8</a>","ista":"Ben Simon Y, Käfer K, Velicky P, Csicsvari JL, Danzl JG, Jonas PM. 2022. A direct excitatory projection from entorhinal layer 6b neurons to the hippocampus contributes to spatial coding and memory. Nature Communications. 13, 4826."},"day":"16","has_accepted_license":"1","article_number":"4826","status":"public","abstract":[{"lang":"eng","text":"The mammalian hippocampal formation (HF) plays a key role in several higher brain functions, such as spatial coding, learning and memory. Its simple circuit architecture is often viewed as a trisynaptic loop, processing input originating from the superficial layers of the entorhinal cortex (EC) and sending it back to its deeper layers. Here, we show that excitatory neurons in layer 6b of the mouse EC project to all sub-regions comprising the HF and receive input from the CA1, thalamus and claustrum. Furthermore, their output is characterized by unique slow-decaying excitatory postsynaptic currents capable of driving plateau-like potentials in their postsynaptic targets. Optogenetic inhibition of the EC-6b pathway affects spatial coding in CA1 pyramidal neurons, while cell ablation impairs not only acquisition of new spatial memories, but also degradation of previously acquired ones. Our results provide evidence of a functional role for cortical layer 6b neurons in the adult brain."}],"ec_funded":1,"department":[{"_id":"JoCs"},{"_id":"PeJo"},{"_id":"JoDa"}],"acknowledgement":"We thank F. Marr and A. Schlögl for technical assistance, E. Kralli-Beller for manuscript editing, as well as C. Sommer and the Imaging and Optics Facility of the Institute of Science and Technology Austria (ISTA) for image analysis scripts and microscopy support. We extend our gratitude to J. Wallenschus and D. Rangel Guerrero for technical assistance acquiring single-unit data and I. Gridchyn for help with single-unit clustering. Finally, we also thank B. Suter for discussions, A. Saunders, M. Jösch, and H. Monyer for critically reading earlier versions of the manuscript, C. Petersen for sharing clearing protocols, and the Scientific Service Units of ISTA for efficient support. This project was funded by the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme (ERC advanced grant No 692692 to P.J.) and the Fond zur Förderung der Wissenschaftlichen Forschung (Z 312-B27, Wittgenstein award for P.J. and I3600-B27 for J.G.D. and P.V.).","title":"A direct excitatory projection from entorhinal layer 6b neurons to the hippocampus contributes to spatial coding and memory","oa":1,"publication_status":"published","article_type":"original","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","month":"08","_id":"11951","publication_identifier":{"issn":["2041-1723"]},"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"article_processing_charge":"No","volume":13,"file":[{"access_level":"open_access","date_updated":"2022-08-26T11:51:40Z","success":1,"content_type":"application/pdf","date_created":"2022-08-26T11:51:40Z","file_id":"11990","file_name":"2022_NatureCommunications_BenSimon.pdf","creator":"dernst","file_size":5910357,"checksum":"405936d9e4d33625d80c093c9713a91f","relation":"main_file"}],"isi":1,"quality_controlled":"1","date_created":"2022-08-24T08:25:50Z","year":"2022","date_updated":"2023-08-03T13:01:19Z","project":[{"call_identifier":"H2020","_id":"25B7EB9E-B435-11E9-9278-68D0E5697425","grant_number":"692692","name":"Biophysics and circuit function of a giant cortical glumatergic synapse"},{"call_identifier":"FWF","_id":"265CB4D0-B435-11E9-9278-68D0E5697425","name":"Optical control of synaptic function via adhesion molecules","grant_number":"I03600"},{"_id":"25C5A090-B435-11E9-9278-68D0E5697425","grant_number":"Z00312","name":"The Wittgenstein Prize","call_identifier":"FWF"}],"acknowledged_ssus":[{"_id":"Bio"},{"_id":"SSU"}],"publication":"Nature Communications","ddc":["570"],"author":[{"id":"43DF3136-F248-11E8-B48F-1D18A9856A87","last_name":"Ben Simon","first_name":"Yoav","full_name":"Ben Simon, Yoav"},{"id":"2DAA49AA-F248-11E8-B48F-1D18A9856A87","first_name":"Karola","full_name":"Käfer, Karola","last_name":"Käfer"},{"full_name":"Velicky, Philipp","first_name":"Philipp","last_name":"Velicky","orcid":"0000-0002-2340-7431","id":"39BDC62C-F248-11E8-B48F-1D18A9856A87"},{"id":"3FA14672-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-5193-4036","first_name":"Jozsef L","full_name":"Csicsvari, Jozsef L","last_name":"Csicsvari"},{"orcid":"0000-0001-8559-3973","id":"42EFD3B6-F248-11E8-B48F-1D18A9856A87","last_name":"Danzl","full_name":"Danzl, Johann G","first_name":"Johann G"},{"first_name":"Peter M","full_name":"Jonas, Peter M","last_name":"Jonas","orcid":"0000-0001-5001-4804","id":"353C1B58-F248-11E8-B48F-1D18A9856A87"}],"oa_version":"Published Version","external_id":{"isi":["000841396400008"]},"date_published":"2022-08-16T00:00:00Z","language":[{"iso":"eng"}],"file_date_updated":"2022-08-26T11:51:40Z","type":"journal_article","doi":"10.1038/s41467-022-32559-8","publisher":"Springer Nature"},{"language":[{"iso":"eng"}],"file_date_updated":"2023-01-23T11:17:33Z","type":"journal_article","doi":"10.1038/s41467-022-34723-6","publisher":"Springer Nature","publication":"Nature Communications","ddc":["580"],"author":[{"full_name":"Huang, Jian","first_name":"Jian","last_name":"Huang"},{"last_name":"Zhao","full_name":"Zhao, Lei","first_name":"Lei"},{"last_name":"Malik","full_name":"Malik, Shikha","first_name":"Shikha"},{"last_name":"Gentile","full_name":"Gentile, Benjamin R.","first_name":"Benjamin R."},{"full_name":"Xiong, Va","first_name":"Va","last_name":"Xiong"},{"full_name":"Arazi, Tzahi","first_name":"Tzahi","last_name":"Arazi"},{"first_name":"Heather A.","full_name":"Owen, Heather A.","last_name":"Owen"},{"last_name":"Friml","first_name":"Jiří","full_name":"Friml, Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596"},{"first_name":"Dazhong","full_name":"Zhao, Dazhong","last_name":"Zhao"}],"oa_version":"Published Version","external_id":{"isi":["000884426700001"],"pmid":["36379956"]},"date_published":"2022-11-15T00:00:00Z","volume":13,"file":[{"date_updated":"2023-01-23T11:17:33Z","access_level":"open_access","success":1,"date_created":"2023-01-23T11:17:33Z","content_type":"application/pdf","file_id":"12346","file_name":"2022_NatureCommunications_Huang.pdf","creator":"dernst","file_size":3375249,"checksum":"233922a7b9507d9d48591e6799e4526e","relation":"main_file"}],"isi":1,"quality_controlled":"1","year":"2022","date_created":"2023-01-12T12:02:41Z","date_updated":"2023-08-04T08:52:01Z","publication_identifier":{"issn":["2041-1723"]},"scopus_import":"1","pmid":1,"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"article_processing_charge":"No","title":"Specification of female germline by microRNA orchestrated auxin signaling in Arabidopsis","oa":1,"publication_status":"published","article_type":"original","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","_id":"12130","month":"11","department":[{"_id":"JiFr"}],"acknowledgement":"We thank A. Cheung,W. Lukowitz, V.Walbot, D.Weijers, and R. Yadegari for critically reading the manuscript; E. Xiong and G. Zhang for preparing some experiments, T. Schuck, J. Gonnering, and P. Engevold for plant care, the Arabidopsis Biological Resource Center (ABRC) for ARF10,ARF16, ARF17, EMS1,MIR160a BAC clones and cDNAs, the SALK_090804 seed, T. Nakagawa for pGBW vectors, Y. Zhao for the YUC1 cDNA, Q. Chen for the pHEE401E vector, R. Yadegari for pAT5G01860::n1GFP, pAT5G45980:n1GFP, pAT5G50490::n1GFP, pAT5G56200:n1GFP vectors, and D.Weijers for the pGreenII KAN SV40-3×GFP and R2D2 vectors, W. Yang for the splmutant, Y. Qin for the pKNU::KNU-VENUS vector and seed, G. Tang for the STTM160/160-48 vector, and L. Colombo for pPIN1::PIN1-GFP spl and pin1-5 seeds. This work was supported by the US National Science Foundation (NSF)-Israel Binational Science Foundation (BSF) research grant to D.Z. (IOS-1322796) and T.A. (2012756). D.Z. also\r\ngratefully acknowledges supports of the Shaw Scientist Award from the Greater Milwaukee Foundation, USDA National Institute of Food and Agriculture (NIFA, 2022-67013-36294), the UWM Discovery and Innovation Grant, the Bradley Catalyst Award from the UWM Research\r\nFoundation, and WiSys and UW System Applied Research Funding Programs.","article_number":"6960","status":"public","abstract":[{"text":"Germline determination is essential for species survival and evolution in multicellular organisms. In most flowering plants, formation of the female germline is initiated with specification of one megaspore mother cell (MMC) in each ovule; however, the molecular mechanism underlying this key event remains unclear. Here we report that spatially restricted auxin signaling promotes MMC fate in Arabidopsis. Our results show that the microRNA160 (miR160) targeted gene ARF17 (AUXIN RESPONSE FACTOR17) is required for promoting MMC specification by genetically interacting with the SPL/NZZ (SPOROCYTELESS/NOZZLE) gene. Alterations of auxin signaling cause formation of supernumerary MMCs in an ARF17- and SPL/NZZ-dependent manner. Furthermore, miR160 and ARF17 are indispensable for attaining a normal auxin maximum at the ovule apex via modulating the expression domain of PIN1 (PIN-FORMED1) auxin transporter. Our findings elucidate the mechanism by which auxin signaling promotes the acquisition of female germline cell fate in plants.","lang":"eng"}],"keyword":["General Physics and Astronomy","General Biochemistry","Genetics and Molecular Biology","General Chemistry","Multidisciplinary"],"intvolume":"        13","citation":{"apa":"Huang, J., Zhao, L., Malik, S., Gentile, B. R., Xiong, V., Arazi, T., … Zhao, D. (2022). Specification of female germline by microRNA orchestrated auxin signaling in Arabidopsis. <i>Nature Communications</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41467-022-34723-6\">https://doi.org/10.1038/s41467-022-34723-6</a>","ista":"Huang J, Zhao L, Malik S, Gentile BR, Xiong V, Arazi T, Owen HA, Friml J, Zhao D. 2022. Specification of female germline by microRNA orchestrated auxin signaling in Arabidopsis. Nature Communications. 13, 6960.","chicago":"Huang, Jian, Lei Zhao, Shikha Malik, Benjamin R. Gentile, Va Xiong, Tzahi Arazi, Heather A. Owen, Jiří Friml, and Dazhong Zhao. “Specification of Female Germline by MicroRNA Orchestrated Auxin Signaling in Arabidopsis.” <i>Nature Communications</i>. Springer Nature, 2022. <a href=\"https://doi.org/10.1038/s41467-022-34723-6\">https://doi.org/10.1038/s41467-022-34723-6</a>.","ieee":"J. Huang <i>et al.</i>, “Specification of female germline by microRNA orchestrated auxin signaling in Arabidopsis,” <i>Nature Communications</i>, vol. 13. Springer Nature, 2022.","mla":"Huang, Jian, et al. “Specification of Female Germline by MicroRNA Orchestrated Auxin Signaling in Arabidopsis.” <i>Nature Communications</i>, vol. 13, 6960, Springer Nature, 2022, doi:<a href=\"https://doi.org/10.1038/s41467-022-34723-6\">10.1038/s41467-022-34723-6</a>.","short":"J. Huang, L. Zhao, S. Malik, B.R. Gentile, V. Xiong, T. Arazi, H.A. Owen, J. Friml, D. Zhao, Nature Communications 13 (2022).","ama":"Huang J, Zhao L, Malik S, et al. Specification of female germline by microRNA orchestrated auxin signaling in Arabidopsis. <i>Nature Communications</i>. 2022;13. doi:<a href=\"https://doi.org/10.1038/s41467-022-34723-6\">10.1038/s41467-022-34723-6</a>"},"day":"15","has_accepted_license":"1"},{"doi":"10.1038/s41467-021-23073-4","publisher":"Springer Nature","language":[{"iso":"eng"}],"type":"journal_article","oa_version":"Published Version","date_published":"2021-05-17T00:00:00Z","publication":"Nature Communications","author":[{"full_name":"Miles, Evan","first_name":"Evan","last_name":"Miles"},{"first_name":"Michael","full_name":"McCarthy, Michael","last_name":"McCarthy"},{"full_name":"Dehecq, Amaury","first_name":"Amaury","last_name":"Dehecq"},{"last_name":"Kneib","first_name":"Marin","full_name":"Kneib, Marin"},{"last_name":"Fugger","full_name":"Fugger, Stefan","first_name":"Stefan"},{"id":"b28f055a-81ea-11ed-b70c-a9fe7f7b0e70","last_name":"Pellicciotti","first_name":"Francesca","full_name":"Pellicciotti, Francesca"}],"quality_controlled":"1","date_created":"2023-02-20T08:11:29Z","year":"2021","date_updated":"2023-02-28T13:21:51Z","volume":12,"article_processing_charge":"No","publication_identifier":{"issn":["2041-1723"]},"scopus_import":"1","main_file_link":[{"open_access":"1","url":"https://doi.org/10.1038/s41467-021-23073-4"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","month":"05","_id":"12585","title":"Health and sustainability of glaciers in High Mountain Asia","oa":1,"publication_status":"published","article_type":"original","article_number":"2868","status":"public","abstract":[{"text":"Glaciers in High Mountain Asia generate meltwater that supports the water needs of 250 million people, but current knowledge of annual accumulation and ablation is limited to sparse field measurements biased in location and glacier size. Here, we present altitudinally-resolved specific mass balances (surface, internal, and basal combined) for 5527 glaciers in High Mountain Asia for 2000–2016, derived by correcting observed glacier thinning patterns for mass redistribution due to ice flow. We find that 41% of glaciers accumulated mass over less than 20% of their area, and only 60% ± 10% of regional annual ablation was compensated by accumulation. Even without 21st century warming, 21% ± 1% of ice volume will be lost by 2100 due to current climatic-geometric imbalance, representing a reduction in glacier ablation into rivers of 28% ± 1%. The ablation of glaciers in the Himalayas and Tien Shan was mostly unsustainable and ice volume in these regions will reduce by at least 30% by 2100. The most important and vulnerable glacier-fed river basins (Amu Darya, Indus, Syr Darya, Tarim Interior) were supplied with >50% sustainable glacier ablation but will see long-term reductions in ice mass and glacier meltwater supply regardless of the Karakoram Anomaly.","lang":"eng"}],"extern":"1","day":"17","keyword":["General Physics and Astronomy","General Biochemistry","Genetics and Molecular Biology","General Chemistry","Multidisciplinary"],"intvolume":"        12","citation":{"mla":"Miles, Evan, et al. “Health and Sustainability of Glaciers in High Mountain Asia.” <i>Nature Communications</i>, vol. 12, 2868, Springer Nature, 2021, doi:<a href=\"https://doi.org/10.1038/s41467-021-23073-4\">10.1038/s41467-021-23073-4</a>.","ieee":"E. Miles, M. McCarthy, A. Dehecq, M. Kneib, S. Fugger, and F. Pellicciotti, “Health and sustainability of glaciers in High Mountain Asia,” <i>Nature Communications</i>, vol. 12. Springer Nature, 2021.","chicago":"Miles, Evan, Michael McCarthy, Amaury Dehecq, Marin Kneib, Stefan Fugger, and Francesca Pellicciotti. “Health and Sustainability of Glaciers in High Mountain Asia.” <i>Nature Communications</i>. Springer Nature, 2021. <a href=\"https://doi.org/10.1038/s41467-021-23073-4\">https://doi.org/10.1038/s41467-021-23073-4</a>.","ista":"Miles E, McCarthy M, Dehecq A, Kneib M, Fugger S, Pellicciotti F. 2021. Health and sustainability of glaciers in High Mountain Asia. Nature Communications. 12, 2868.","apa":"Miles, E., McCarthy, M., Dehecq, A., Kneib, M., Fugger, S., &#38; Pellicciotti, F. (2021). Health and sustainability of glaciers in High Mountain Asia. <i>Nature Communications</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41467-021-23073-4\">https://doi.org/10.1038/s41467-021-23073-4</a>","ama":"Miles E, McCarthy M, Dehecq A, Kneib M, Fugger S, Pellicciotti F. Health and sustainability of glaciers in High Mountain Asia. <i>Nature Communications</i>. 2021;12. doi:<a href=\"https://doi.org/10.1038/s41467-021-23073-4\">10.1038/s41467-021-23073-4</a>","short":"E. Miles, M. McCarthy, A. Dehecq, M. Kneib, S. Fugger, F. Pellicciotti, Nature Communications 12 (2021)."}},{"abstract":[{"lang":"eng","text":"Aprotic alkali metal–O2 batteries face two major obstacles to their chemistry occurring efficiently, the insulating nature of the formed alkali superoxides/peroxides and parasitic reactions that are caused by the highly reactive singlet oxygen (1O2). Redox mediators are recognized to be key for improving rechargeability. However, it is unclear how they affect 1O2 formation, which hinders strategies for their improvement. Here we clarify the mechanism of mediated peroxide and superoxide oxidation and thus explain how redox mediators either enhance or suppress 1O2 formation. We show that charging commences with peroxide oxidation to a superoxide intermediate and that redox potentials above ~3.5 V versus Li/Li+ drive 1O2 evolution from superoxide oxidation, while disproportionation always generates some 1O2. We find that 1O2 suppression requires oxidation to be faster than the generation of 1O2 from disproportionation. Oxidation rates decrease with growing driving force following Marcus inverted-region behaviour, establishing a region of maximum rate."}],"status":"public","citation":{"short":"Y.K. Petit, E. Mourad, C. Prehal, C. Leypold, A. Windischbacher, D. Mijailovic, C. Slugovc, S.M. Borisov, E. Zojer, S. Brutti, O. Fontaine, S.A. Freunberger, Nature Chemistry 13 (2021) 465–471.","ama":"Petit YK, Mourad E, Prehal C, et al. Mechanism of mediated alkali peroxide oxidation and triplet versus singlet oxygen formation. <i>Nature Chemistry</i>. 2021;13(5):465-471. doi:<a href=\"https://doi.org/10.1038/s41557-021-00643-z\">10.1038/s41557-021-00643-z</a>","apa":"Petit, Y. K., Mourad, E., Prehal, C., Leypold, C., Windischbacher, A., Mijailovic, D., … Freunberger, S. A. (2021). Mechanism of mediated alkali peroxide oxidation and triplet versus singlet oxygen formation. <i>Nature Chemistry</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41557-021-00643-z\">https://doi.org/10.1038/s41557-021-00643-z</a>","chicago":"Petit, Yann K., Eléonore Mourad, Christian Prehal, Christian Leypold, Andreas Windischbacher, Daniel Mijailovic, Christian Slugovc, et al. “Mechanism of Mediated Alkali Peroxide Oxidation and Triplet versus Singlet Oxygen Formation.” <i>Nature Chemistry</i>. Springer Nature, 2021. <a href=\"https://doi.org/10.1038/s41557-021-00643-z\">https://doi.org/10.1038/s41557-021-00643-z</a>.","ista":"Petit YK, Mourad E, Prehal C, Leypold C, Windischbacher A, Mijailovic D, Slugovc C, Borisov SM, Zojer E, Brutti S, Fontaine O, Freunberger SA. 2021. Mechanism of mediated alkali peroxide oxidation and triplet versus singlet oxygen formation. Nature Chemistry. 13(5), 465–471.","ieee":"Y. K. Petit <i>et al.</i>, “Mechanism of mediated alkali peroxide oxidation and triplet versus singlet oxygen formation,” <i>Nature Chemistry</i>, vol. 13, no. 5. Springer Nature, pp. 465–471, 2021.","mla":"Petit, Yann K., et al. “Mechanism of Mediated Alkali Peroxide Oxidation and Triplet versus Singlet Oxygen Formation.” <i>Nature Chemistry</i>, vol. 13, no. 5, Springer Nature, 2021, pp. 465–71, doi:<a href=\"https://doi.org/10.1038/s41557-021-00643-z\">10.1038/s41557-021-00643-z</a>."},"intvolume":"        13","keyword":["General Chemistry","General Chemical Engineering"],"has_accepted_license":"1","day":"15","article_type":"original","oa":1,"title":"Mechanism of mediated alkali peroxide oxidation and triplet versus singlet oxygen formation","publication_status":"published","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","month":"03","_id":"9250","acknowledgement":"S.A.F. is indebted to the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme (grant agreement No. 636069) as well as IST Austria. O.F thanks the French National Research Agency (STORE-EX Labex Project ANR-10-LABX-76-01). We thank EL-Cell GmbH (Hamburg, Germany) for the pressure test cell. We thank R. Saf for help with the mass spectrometry, J. Schlegl for manufacturing instrumentation, M. Winkler of Acib GmbH, G. Strohmeier and R. Fürst for HPLC measurements and S. Mondal and S. Stadlbauer for kinetic measurements.","department":[{"_id":"StFr"}],"volume":13,"file":[{"file_id":"9276","date_created":"2021-03-22T11:46:00Z","content_type":"application/pdf","date_updated":"2021-09-16T22:30:03Z","access_level":"open_access","file_name":"2021_NatureChem_Petit_acceptedVersion.pdf","embargo":"2021-09-15","file_size":1811448,"creator":"dernst","relation":"main_file","checksum":"3ee3f8dd79ed1b7bb0929fce184c8012"}],"isi":1,"date_updated":"2023-09-05T15:34:44Z","acknowledged_ssus":[{"_id":"M-Shop"}],"page":"465-471","quality_controlled":"1","date_created":"2021-03-16T11:12:20Z","year":"2021","scopus_import":"1","pmid":1,"publication_identifier":{"eissn":["1755-4349"],"issn":["1755-4330"]},"article_processing_charge":"No","file_date_updated":"2021-09-16T22:30:03Z","type":"journal_article","language":[{"iso":"eng"}],"publisher":"Springer Nature","doi":"10.1038/s41557-021-00643-z","ddc":["540"],"author":[{"last_name":"Petit","full_name":"Petit, Yann K.","first_name":"Yann K."},{"first_name":"Eléonore","full_name":"Mourad, Eléonore","last_name":"Mourad"},{"first_name":"Christian","full_name":"Prehal, Christian","last_name":"Prehal"},{"last_name":"Leypold","first_name":"Christian","full_name":"Leypold, Christian"},{"full_name":"Windischbacher, Andreas","first_name":"Andreas","last_name":"Windischbacher"},{"first_name":"Daniel","full_name":"Mijailovic, Daniel","last_name":"Mijailovic"},{"last_name":"Slugovc","first_name":"Christian","full_name":"Slugovc, Christian"},{"last_name":"Borisov","first_name":"Sergey M.","full_name":"Borisov, Sergey M."},{"first_name":"Egbert","full_name":"Zojer, Egbert","last_name":"Zojer"},{"last_name":"Brutti","first_name":"Sergio","full_name":"Brutti, Sergio"},{"last_name":"Fontaine","full_name":"Fontaine, Olivier","first_name":"Olivier"},{"first_name":"Stefan Alexander","full_name":"Freunberger, Stefan Alexander","last_name":"Freunberger","orcid":"0000-0003-2902-5319","id":"A8CA28E6-CE23-11E9-AD2D-EC27E6697425"}],"publication":"Nature Chemistry","issue":"5","date_published":"2021-03-15T00:00:00Z","external_id":{"isi":["000629296400001"],"pmid":["33723377"]},"oa_version":"Submitted Version"},{"oa":1,"title":"Complete mapping of magnetic anisotropy for prototype Ising van der Waals FePS3","publication_status":"published","article_type":"original","user_id":"8b945eb4-e2f2-11eb-945a-df72226e66a9","month":"04","_id":"9282","department":[{"_id":"KiMo"}],"article_number":"035011","status":"public","abstract":[{"text":"Several Ising-type magnetic van der Waals (vdW) materials exhibit stable magnetic ground states. Despite these clear experimental demonstrations, a complete theoretical and microscopic understanding of their magnetic anisotropy is still lacking. In particular, the validity limit of identifying their one-dimensional (1-D) Ising nature has remained uninvestigated in a quantitative way. Here we performed the complete mapping of magnetic anisotropy for a prototypical Ising vdW magnet FePS3 for the first time. Combining torque magnetometry measurements with their magnetostatic model analysis and the relativistic density functional total energy calculations, we successfully constructed the three-dimensional (3-D) mappings of the magnetic anisotropy in terms of magnetic torque and energy. The results not only quantitatively confirm that the easy axis is perpendicular to the ab plane, but also reveal the anisotropies within the ab, ac, and bc planes. Our approach can be applied to the detailed quantitative study of magnetism in vdW materials.","lang":"eng"}],"extern":"1","keyword":["Mechanical Engineering","General Materials Science","Mechanics of Materials","General Chemistry","Condensed Matter Physics"],"intvolume":"         8","citation":{"short":"M. Nauman, D.H. Kiem, S. Lee, S. Son, J.-G. Park, W. Kang, M.J. Han, Y.J. Jo, 2D Materials 8 (2021).","ama":"Nauman M, Kiem DH, Lee S, et al. Complete mapping of magnetic anisotropy for prototype Ising van der Waals FePS3. <i>2D Materials</i>. 2021;8(3). doi:<a href=\"https://doi.org/10.1088/2053-1583/abeed3\">10.1088/2053-1583/abeed3</a>","ista":"Nauman M, Kiem DH, Lee S, Son S, Park J-G, Kang W, Han MJ, Jo YJ. 2021. Complete mapping of magnetic anisotropy for prototype Ising van der Waals FePS3. 2D Materials. 8(3), 035011.","chicago":"Nauman, Muhammad, Do Hoon Kiem, Sungmin Lee, Suhan Son, J-G Park, Woun Kang, Myung Joon Han, and Youn Jung Jo. “Complete Mapping of Magnetic Anisotropy for Prototype Ising van Der Waals FePS3.” <i>2D Materials</i>. IOP Publishing, 2021. <a href=\"https://doi.org/10.1088/2053-1583/abeed3\">https://doi.org/10.1088/2053-1583/abeed3</a>.","apa":"Nauman, M., Kiem, D. H., Lee, S., Son, S., Park, J.-G., Kang, W., … Jo, Y. J. (2021). Complete mapping of magnetic anisotropy for prototype Ising van der Waals FePS3. <i>2D Materials</i>. IOP Publishing. <a href=\"https://doi.org/10.1088/2053-1583/abeed3\">https://doi.org/10.1088/2053-1583/abeed3</a>","mla":"Nauman, Muhammad, et al. “Complete Mapping of Magnetic Anisotropy for Prototype Ising van Der Waals FePS3.” <i>2D Materials</i>, vol. 8, no. 3, 035011, IOP Publishing, 2021, doi:<a href=\"https://doi.org/10.1088/2053-1583/abeed3\">10.1088/2053-1583/abeed3</a>.","ieee":"M. Nauman <i>et al.</i>, “Complete mapping of magnetic anisotropy for prototype Ising van der Waals FePS3,” <i>2D Materials</i>, vol. 8, no. 3. IOP Publishing, 2021."},"day":"06","arxiv":1,"language":[{"iso":"eng"}],"type":"journal_article","doi":"10.1088/2053-1583/abeed3","publisher":"IOP Publishing","issue":"3","publication":"2D Materials","author":[{"full_name":"Nauman, Muhammad","first_name":"Muhammad","last_name":"Nauman","id":"32c21954-2022-11eb-9d5f-af9f93c24e71","orcid":"0000-0002-2111-4846"},{"last_name":"Kiem","full_name":"Kiem, Do Hoon","first_name":"Do Hoon"},{"last_name":"Lee","first_name":"Sungmin","full_name":"Lee, Sungmin"},{"full_name":"Son, Suhan","first_name":"Suhan","last_name":"Son"},{"full_name":"Park, J-G","first_name":"J-G","last_name":"Park"},{"full_name":"Kang, Woun","first_name":"Woun","last_name":"Kang"},{"full_name":"Han, Myung Joon","first_name":"Myung Joon","last_name":"Han"},{"last_name":"Jo","first_name":"Youn Jung","full_name":"Jo, Youn Jung"}],"oa_version":"Preprint","external_id":{"arxiv":["2103.09029"]},"date_published":"2021-04-06T00:00:00Z","volume":8,"quality_controlled":"1","year":"2021","date_created":"2021-03-23T07:10:17Z","date_updated":"2021-12-01T10:36:56Z","publication_identifier":{"issn":["2053-1583"]},"main_file_link":[{"open_access":"1","url":"https://arxiv.org/abs/2103.09029"}],"article_processing_charge":"No"},{"title":"Electrostatic co-assembly of nanoparticles with oppositely charged small molecules into static and dynamic superstructures","oa":1,"publication_status":"published","article_type":"original","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","_id":"13357","month":"10","status":"public","abstract":[{"lang":"eng","text":"Coulombic interactions can be used to assemble charged nanoparticles into higher-order structures, but the process requires oppositely charged partners that are similarly sized. The ability to mediate the assembly of such charged nanoparticles using structurally simple small molecules would greatly facilitate the fabrication of nanostructured materials and harnessing their applications in catalysis, sensing and photonics. Here we show that small molecules with as few as three electric charges can effectively induce attractive interactions between oppositely charged nanoparticles in water. These interactions can guide the assembly of charged nanoparticles into colloidal crystals of a quality previously only thought to result from their co-crystallization with oppositely charged nanoparticles of a similar size. Transient nanoparticle assemblies can be generated using positively charged nanoparticles and multiply charged anions that are enzymatically hydrolysed into mono- and/or dianions. Our findings demonstrate an approach for the facile fabrication, manipulation and further investigation of static and dynamic nanostructured materials in aqueous environments."}],"extern":"1","keyword":["General Chemical Engineering","General Chemistry"],"citation":{"ama":"Bian T, Gardin A, Gemen J, et al. Electrostatic co-assembly of nanoparticles with oppositely charged small molecules into static and dynamic superstructures. <i>Nature Chemistry</i>. 2021;13(10):940-949. doi:<a href=\"https://doi.org/10.1038/s41557-021-00752-9\">10.1038/s41557-021-00752-9</a>","short":"T. Bian, A. Gardin, J. Gemen, L. Houben, C. Perego, B. Lee, N. Elad, Z. Chu, G.M. Pavan, R. Klajn, Nature Chemistry 13 (2021) 940–949.","ieee":"T. Bian <i>et al.</i>, “Electrostatic co-assembly of nanoparticles with oppositely charged small molecules into static and dynamic superstructures,” <i>Nature Chemistry</i>, vol. 13, no. 10. Springer Nature, pp. 940–949, 2021.","mla":"Bian, Tong, et al. “Electrostatic Co-Assembly of Nanoparticles with Oppositely Charged Small Molecules into Static and Dynamic Superstructures.” <i>Nature Chemistry</i>, vol. 13, no. 10, Springer Nature, 2021, pp. 940–49, doi:<a href=\"https://doi.org/10.1038/s41557-021-00752-9\">10.1038/s41557-021-00752-9</a>.","ista":"Bian T, Gardin A, Gemen J, Houben L, Perego C, Lee B, Elad N, Chu Z, Pavan GM, Klajn R. 2021. Electrostatic co-assembly of nanoparticles with oppositely charged small molecules into static and dynamic superstructures. Nature Chemistry. 13(10), 940–949.","chicago":"Bian, Tong, Andrea Gardin, Julius Gemen, Lothar Houben, Claudio Perego, Byeongdu Lee, Nadav Elad, Zonglin Chu, Giovanni M. Pavan, and Rafal Klajn. “Electrostatic Co-Assembly of Nanoparticles with Oppositely Charged Small Molecules into Static and Dynamic Superstructures.” <i>Nature Chemistry</i>. Springer Nature, 2021. <a href=\"https://doi.org/10.1038/s41557-021-00752-9\">https://doi.org/10.1038/s41557-021-00752-9</a>.","apa":"Bian, T., Gardin, A., Gemen, J., Houben, L., Perego, C., Lee, B., … Klajn, R. (2021). Electrostatic co-assembly of nanoparticles with oppositely charged small molecules into static and dynamic superstructures. <i>Nature Chemistry</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41557-021-00752-9\">https://doi.org/10.1038/s41557-021-00752-9</a>"},"intvolume":"        13","day":"01","language":[{"iso":"eng"}],"type":"journal_article","doi":"10.1038/s41557-021-00752-9","publisher":"Springer Nature","issue":"10","publication":"Nature Chemistry","author":[{"first_name":"Tong","full_name":"Bian, Tong","last_name":"Bian"},{"first_name":"Andrea","full_name":"Gardin, Andrea","last_name":"Gardin"},{"full_name":"Gemen, Julius","first_name":"Julius","last_name":"Gemen"},{"last_name":"Houben","first_name":"Lothar","full_name":"Houben, Lothar"},{"full_name":"Perego, Claudio","first_name":"Claudio","last_name":"Perego"},{"last_name":"Lee","full_name":"Lee, Byeongdu","first_name":"Byeongdu"},{"full_name":"Elad, Nadav","first_name":"Nadav","last_name":"Elad"},{"last_name":"Chu","full_name":"Chu, Zonglin","first_name":"Zonglin"},{"first_name":"Giovanni M.","full_name":"Pavan, Giovanni M.","last_name":"Pavan"},{"last_name":"Klajn","full_name":"Klajn, Rafal","first_name":"Rafal","id":"8e84690e-1e48-11ed-a02b-a1e6fb8bb53b"}],"external_id":{"pmid":["34489564"]},"oa_version":"Published Version","date_published":"2021-10-01T00:00:00Z","volume":13,"page":"940-949","quality_controlled":"1","year":"2021","date_created":"2023-08-01T09:34:54Z","date_updated":"2023-08-02T10:55:29Z","publication_identifier":{"issn":["1755-4330"],"eissn":["1755-4349"]},"scopus_import":"1","pmid":1,"main_file_link":[{"open_access":"1","url":"https://doi.org/10.1038/s41557-021-00752-9"}],"article_processing_charge":"No"},{"type":"journal_article","language":[{"iso":"eng"}],"publisher":"Wiley","doi":"10.1002/anie.202014963","author":[{"full_name":"Ryssy, Joonas","first_name":"Joonas","last_name":"Ryssy"},{"first_name":"Ashwin K.","full_name":"Natarajan, Ashwin K.","last_name":"Natarajan"},{"full_name":"Wang, Jinhua","first_name":"Jinhua","last_name":"Wang"},{"first_name":"Arttu J.","full_name":"Lehtonen, Arttu J.","last_name":"Lehtonen"},{"last_name":"Nguyen","full_name":"Nguyen, Minh‐Kha","first_name":"Minh‐Kha"},{"id":"8e84690e-1e48-11ed-a02b-a1e6fb8bb53b","full_name":"Klajn, Rafal","first_name":"Rafal","last_name":"Klajn"},{"last_name":"Kuzyk","full_name":"Kuzyk, Anton","first_name":"Anton"}],"publication":"Angewandte Chemie International Edition","issue":"11","date_published":"2021-03-08T00:00:00Z","oa_version":"Published Version","volume":60,"date_updated":"2023-08-02T07:22:23Z","date_created":"2023-08-01T09:35:06Z","year":"2021","page":"5859-5863","quality_controlled":"1","main_file_link":[{"url":"https://doi.org/10.1002/anie.202014963","open_access":"1"}],"scopus_import":"1","publication_identifier":{"issn":["1433-7851"],"eissn":["1521-3773"]},"article_processing_charge":"No","article_type":"original","publication_status":"published","title":"Light‐responsive dynamic DNA‐origami‐based plasmonic assemblies","related_material":{"link":[{"relation":"erratum","url":"https://doi.org/10.1002/anie.202210394"}]},"oa":1,"month":"03","_id":"13358","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","extern":"1","abstract":[{"text":"DNA nanotechnology offers a versatile toolbox for precise spatial and temporal manipulation of matter on the nanoscale. However, rendering DNA-based systems responsive to light has remained challenging. Herein, we describe the remote manipulation of native (non-photoresponsive) chiral plasmonic molecules (CPMs) using light. Our strategy is based on the use of a photoresponsive medium comprising a merocyanine-based photoacid. Upon exposure to visible light, the medium decreases its pH, inducing the formation of DNA triplex links, leading to a spatial reconfiguration of the CPMs. The process can be reversed simply by turning the light off and it can be repeated for multiple cycles. The degree of the overall chirality change in an ensemble of CPMs depends on the CPM fraction undergoing reconfiguration, which, remarkably, depends on and can be tuned by the intensity of incident light. Such a dynamic, remotely controlled system could aid in further advancing DNA-based devices and nanomaterials.","lang":"eng"}],"status":"public","intvolume":"        60","citation":{"chicago":"Ryssy, Joonas, Ashwin K. Natarajan, Jinhua Wang, Arttu J. Lehtonen, Minh‐Kha Nguyen, Rafal Klajn, and Anton Kuzyk. “Light‐responsive Dynamic DNA‐origami‐based Plasmonic Assemblies.” <i>Angewandte Chemie International Edition</i>. Wiley, 2021. <a href=\"https://doi.org/10.1002/anie.202014963\">https://doi.org/10.1002/anie.202014963</a>.","ista":"Ryssy J, Natarajan AK, Wang J, Lehtonen AJ, Nguyen M, Klajn R, Kuzyk A. 2021. Light‐responsive dynamic DNA‐origami‐based plasmonic assemblies. Angewandte Chemie International Edition. 60(11), 5859–5863.","apa":"Ryssy, J., Natarajan, A. K., Wang, J., Lehtonen, A. J., Nguyen, M., Klajn, R., &#38; Kuzyk, A. (2021). Light‐responsive dynamic DNA‐origami‐based plasmonic assemblies. <i>Angewandte Chemie International Edition</i>. Wiley. <a href=\"https://doi.org/10.1002/anie.202014963\">https://doi.org/10.1002/anie.202014963</a>","ieee":"J. Ryssy <i>et al.</i>, “Light‐responsive dynamic DNA‐origami‐based plasmonic assemblies,” <i>Angewandte Chemie International Edition</i>, vol. 60, no. 11. Wiley, pp. 5859–5863, 2021.","mla":"Ryssy, Joonas, et al. “Light‐responsive Dynamic DNA‐origami‐based Plasmonic Assemblies.” <i>Angewandte Chemie International Edition</i>, vol. 60, no. 11, Wiley, 2021, pp. 5859–63, doi:<a href=\"https://doi.org/10.1002/anie.202014963\">10.1002/anie.202014963</a>.","short":"J. Ryssy, A.K. Natarajan, J. Wang, A.J. Lehtonen, M. Nguyen, R. Klajn, A. Kuzyk, Angewandte Chemie International Edition 60 (2021) 5859–5863.","ama":"Ryssy J, Natarajan AK, Wang J, et al. Light‐responsive dynamic DNA‐origami‐based plasmonic assemblies. <i>Angewandte Chemie International Edition</i>. 2021;60(11):5859-5863. doi:<a href=\"https://doi.org/10.1002/anie.202014963\">10.1002/anie.202014963</a>"},"keyword":["General Chemistry","Catalysis"],"day":"08"},{"author":[{"last_name":"Weißenfels","full_name":"Weißenfels, Maren","first_name":"Maren"},{"last_name":"Gemen","first_name":"Julius","full_name":"Gemen, Julius"},{"id":"8e84690e-1e48-11ed-a02b-a1e6fb8bb53b","full_name":"Klajn, Rafal","first_name":"Rafal","last_name":"Klajn"}],"publication":"Chem","issue":"1","date_published":"2021-01-14T00:00:00Z","oa_version":"Published Version","type":"journal_article","language":[{"iso":"eng"}],"publisher":"Elsevier","doi":"10.1016/j.chempr.2020.11.025","main_file_link":[{"open_access":"1","url":"https://doi.org/10.1016/j.chempr.2020.11.025"}],"scopus_import":"1","publication_identifier":{"issn":["2451-9294"]},"article_processing_charge":"No","volume":7,"date_updated":"2023-08-07T10:04:28Z","year":"2021","date_created":"2023-08-01T09:35:19Z","page":"23-37","quality_controlled":"1","article_type":"original","publication_status":"published","oa":1,"title":"Dissipative self-assembly: Fueling with chemicals versus light","month":"01","_id":"13359","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","intvolume":"         7","citation":{"apa":"Weißenfels, M., Gemen, J., &#38; Klajn, R. (2021). Dissipative self-assembly: Fueling with chemicals versus light. <i>Chem</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.chempr.2020.11.025\">https://doi.org/10.1016/j.chempr.2020.11.025</a>","chicago":"Weißenfels, Maren, Julius Gemen, and Rafal Klajn. “Dissipative Self-Assembly: Fueling with Chemicals versus Light.” <i>Chem</i>. Elsevier, 2021. <a href=\"https://doi.org/10.1016/j.chempr.2020.11.025\">https://doi.org/10.1016/j.chempr.2020.11.025</a>.","ista":"Weißenfels M, Gemen J, Klajn R. 2021. Dissipative self-assembly: Fueling with chemicals versus light. Chem. 7(1), 23–37.","mla":"Weißenfels, Maren, et al. “Dissipative Self-Assembly: Fueling with Chemicals versus Light.” <i>Chem</i>, vol. 7, no. 1, Elsevier, 2021, pp. 23–37, doi:<a href=\"https://doi.org/10.1016/j.chempr.2020.11.025\">10.1016/j.chempr.2020.11.025</a>.","ieee":"M. Weißenfels, J. Gemen, and R. Klajn, “Dissipative self-assembly: Fueling with chemicals versus light,” <i>Chem</i>, vol. 7, no. 1. Elsevier, pp. 23–37, 2021.","short":"M. Weißenfels, J. Gemen, R. Klajn, Chem 7 (2021) 23–37.","ama":"Weißenfels M, Gemen J, Klajn R. Dissipative self-assembly: Fueling with chemicals versus light. <i>Chem</i>. 2021;7(1):23-37. doi:<a href=\"https://doi.org/10.1016/j.chempr.2020.11.025\">10.1016/j.chempr.2020.11.025</a>"},"keyword":["Materials Chemistry","Biochemistry (medical)","General Chemical Engineering","Environmental Chemistry","Biochemistry","General Chemistry"],"day":"14","extern":"1","abstract":[{"text":"Dissipative self-assembly is ubiquitous in nature, where it gives rise to complex structures and functions such as self-healing, homeostasis, and camouflage. These phenomena are enabled by the continuous conversion of energy stored in chemical fuels, such as ATP. Over the past decade, an increasing number of synthetic chemically driven systems have been reported that mimic the features of their natural counterparts. At the same time, it has been shown that dissipative self-assembly can also be fueled by light; these optically fueled systems have been developed in parallel to the chemically fueled ones. In this perspective, we critically compare these two classes of systems. Despite the complementarity and fundamental differences between these two modes of dissipative self-assembly, our analysis reveals that multiple analogies exist between chemically and light-fueled systems. We hope that these considerations will facilitate further development of the field of dissipative self-assembly.","lang":"eng"}],"status":"public"},{"type":"journal_article","arxiv":1,"language":[{"iso":"eng"}],"publisher":"American Chemical Society","doi":"10.1021/acs.nanolett.1c02145","author":[{"last_name":"Baykusheva","first_name":"Denitsa Rangelova","full_name":"Baykusheva, Denitsa Rangelova","id":"71b4d059-2a03-11ee-914d-dfa3beed6530"},{"last_name":"Chacón","full_name":"Chacón, Alexis","first_name":"Alexis"},{"full_name":"Lu, Jian","first_name":"Jian","last_name":"Lu"},{"first_name":"Trevor P.","full_name":"Bailey, Trevor P.","last_name":"Bailey"},{"first_name":"Jonathan A.","full_name":"Sobota, Jonathan A.","last_name":"Sobota"},{"last_name":"Soifer","first_name":"Hadas","full_name":"Soifer, Hadas"},{"full_name":"Kirchmann, Patrick S.","first_name":"Patrick S.","last_name":"Kirchmann"},{"first_name":"Costel","full_name":"Rotundu, Costel","last_name":"Rotundu"},{"full_name":"Uher, Ctirad","first_name":"Ctirad","last_name":"Uher"},{"full_name":"Heinz, Tony F.","first_name":"Tony F.","last_name":"Heinz"},{"last_name":"Reis","first_name":"David A.","full_name":"Reis, David A."},{"last_name":"Ghimire","full_name":"Ghimire, Shambhu","first_name":"Shambhu"}],"issue":"21","publication":"Nano Letters","date_published":"2021-10-22T00:00:00Z","external_id":{"arxiv":["2109.15291"],"pmid":["34676752"]},"oa_version":"Published Version","volume":21,"date_updated":"2023-08-22T07:32:00Z","year":"2021","date_created":"2023-08-09T13:09:15Z","page":"8970-8978","quality_controlled":"1","pmid":1,"main_file_link":[{"url":"https://doi.org/10.1021/acs.nanolett.1c02145","open_access":"1"}],"scopus_import":"1","publication_identifier":{"issn":["1530-6984"],"eissn":["1530-6992"]},"article_processing_charge":"No","article_type":"original","publication_status":"published","oa":1,"title":"All-optical probe of three-dimensional topological insulators based on high-harmonic generation by circularly polarized laser fields","month":"10","_id":"13996","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","extern":"1","abstract":[{"lang":"eng","text":"We report the observation of an anomalous nonlinear optical response of the prototypical three-dimensional topological insulator bismuth selenide through the process of high-order harmonic generation. We find that the generation efficiency increases as the laser polarization is changed from linear to elliptical, and it becomes maximum for circular polarization. With the aid of a microscopic theory and a detailed analysis of the measured spectra, we reveal that such anomalous enhancement encodes the characteristic topology of the band structure that originates from the interplay of strong spin–orbit coupling and time-reversal symmetry protection. The implications are in ultrafast probing of topological phase transitions, light-field driven dissipationless electronics, and quantum computation."}],"status":"public","citation":{"short":"D.R. Baykusheva, A. Chacón, J. Lu, T.P. Bailey, J.A. Sobota, H. Soifer, P.S. Kirchmann, C. Rotundu, C. Uher, T.F. Heinz, D.A. Reis, S. Ghimire, Nano Letters 21 (2021) 8970–8978.","ama":"Baykusheva DR, Chacón A, Lu J, et al. All-optical probe of three-dimensional topological insulators based on high-harmonic generation by circularly polarized laser fields. <i>Nano Letters</i>. 2021;21(21):8970-8978. doi:<a href=\"https://doi.org/10.1021/acs.nanolett.1c02145\">10.1021/acs.nanolett.1c02145</a>","ista":"Baykusheva DR, Chacón A, Lu J, Bailey TP, Sobota JA, Soifer H, Kirchmann PS, Rotundu C, Uher C, Heinz TF, Reis DA, Ghimire S. 2021. All-optical probe of three-dimensional topological insulators based on high-harmonic generation by circularly polarized laser fields. Nano Letters. 21(21), 8970–8978.","apa":"Baykusheva, D. R., Chacón, A., Lu, J., Bailey, T. P., Sobota, J. A., Soifer, H., … Ghimire, S. (2021). All-optical probe of three-dimensional topological insulators based on high-harmonic generation by circularly polarized laser fields. <i>Nano Letters</i>. American Chemical Society. <a href=\"https://doi.org/10.1021/acs.nanolett.1c02145\">https://doi.org/10.1021/acs.nanolett.1c02145</a>","chicago":"Baykusheva, Denitsa Rangelova, Alexis Chacón, Jian Lu, Trevor P. Bailey, Jonathan A. Sobota, Hadas Soifer, Patrick S. Kirchmann, et al. “All-Optical Probe of Three-Dimensional Topological Insulators Based on High-Harmonic Generation by Circularly Polarized Laser Fields.” <i>Nano Letters</i>. American Chemical Society, 2021. <a href=\"https://doi.org/10.1021/acs.nanolett.1c02145\">https://doi.org/10.1021/acs.nanolett.1c02145</a>.","ieee":"D. R. Baykusheva <i>et al.</i>, “All-optical probe of three-dimensional topological insulators based on high-harmonic generation by circularly polarized laser fields,” <i>Nano Letters</i>, vol. 21, no. 21. American Chemical Society, pp. 8970–8978, 2021.","mla":"Baykusheva, Denitsa Rangelova, et al. “All-Optical Probe of Three-Dimensional Topological Insulators Based on High-Harmonic Generation by Circularly Polarized Laser Fields.” <i>Nano Letters</i>, vol. 21, no. 21, American Chemical Society, 2021, pp. 8970–78, doi:<a href=\"https://doi.org/10.1021/acs.nanolett.1c02145\">10.1021/acs.nanolett.1c02145</a>."},"intvolume":"        21","keyword":["Mechanical Engineering","Condensed Matter Physics","General Materials Science","General Chemistry","Bioengineering"],"day":"22"},{"title":"PHF3 regulates neuronal gene expression through the Pol II CTD reader domain SPOC","oa":1,"related_material":{"link":[{"relation":"earlier_version","url":"https://www.biorxiv.org/content/10.1101/2020.02.11.943159","description":"Preprint "}]},"publication_status":"published","article_type":"original","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","_id":"10163","month":"10","department":[{"_id":"CaBe"}],"acknowledgement":"D.S. thanks Claudine Kraft, Renée Schroeder, Verena Jantsch, Franz Klein and Peter Schlögelhofer for support. We thank Anita Testa Salmazo for help with purifying Pol II; Matthias Geyer and Robert Düster for sharing DYRK1A kinase; Felix Hartmann and Clemens Plaschka for help with mass photometry; Goran Kokic for design of the arrest assay sequences; Petra van der Lelij for help with generating mESC KO; Maximilian Freilinger for help with the purification of mEGFP-CTD; Stefan Ameres, Nina Fasching and Brian Reichholf for advice on SLAM-seq and for sharing reagents; Laura Gallego Valle for advice regarding LLPS assays; Krzysztof Chylinski for advice regarding CRISPR/Cas9 methodology; VBCF Protein Technologies facility for purifying PHF3 and providing gRNAs and Cas9; VBCF NGS facility for sequencing; Monoclonal antibody facility at the Helmholtz center for Pol II antibodies; Friedrich Propst and Elzbieta Kowalska for advice and for sharing materials; Egon Ogris for sharing materials; Martin Eilers for recommending a ChIP-grade TFIIS antibody; Susanne Opravil, Otto Hudecz, Markus Hartl and Natascha Hartl for mass spectrometry analysis; staff of the X-ray beamlines at the ESRF in Grenoble for their excellent support; Christa Bücker, Anton Meinhart, Clemens Plaschka and members of the Slade lab for critical comments on the manuscript; Life Science Editors for editing assistance. M.B. and D.S. acknowledge support by the FWF-funded DK ‘Chromosome Dynamics’. T.K. is a recipient of the DOC fellowship from the Austrian Academy of Sciences. U.S. is supported by the L’Oreal for Women in Science Austria Fellowship and the Austrian Science Fund (FWF T 795-B30). M.L is supported by the Vienna Science and Technology Fund (WWTF, VRG14-006). R.S. is supported by the Czech Science Foundation (15-17670 S and 21-24460 S), Ministry of Education, Youths and Sports of the Czech Republic (CEITEC 2020 project (LQ1601)), and the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme (Grant agreement no. 649030); this publication reflects only the author’s view and the Research Executive Agency is not responsible for any use that may be made of the information it contains. M.S. is supported by the Czech Science Foundation (GJ20-21581Y). K.D.C. research is supported by the Austrian Science Fund (FWF) Projects I525 and I1593, P22276, P19060, and W1221, Federal Ministry of Economy, Family and Youth through the initiative ‘Laura Bassi Centres of Expertise’, funding from the Centre of Optimized Structural Studies No. 253275, the Wellcome Trust Collaborative Award (201543/Z/16), COST action BM1405 Non-globular proteins - from sequence to structure, function and application in molecular physiopathology (NGP-NET), the Vienna Science and Technology Fund (WWTF LS17-008), and by the University of Vienna. This project was funded by the MFPL start-up grant, the Vienna Science and Technology Fund (WWTF LS14-001), and the Austrian Science Fund (P31546-B28 and W1258 “DK: Integrative Structural Biology”) to D.S.","article_number":"6078","status":"public","abstract":[{"text":"The C-terminal domain (CTD) of the largest subunit of RNA polymerase II (Pol II) is a regulatory hub for transcription and RNA processing. Here, we identify PHD-finger protein 3 (PHF3) as a regulator of transcription and mRNA stability that docks onto Pol II CTD through its SPOC domain. We characterize SPOC as a CTD reader domain that preferentially binds two phosphorylated Serine-2 marks in adjacent CTD repeats. PHF3 drives liquid-liquid phase separation of phosphorylated Pol II, colocalizes with Pol II clusters and tracks with Pol II across the length of genes. PHF3 knock-out or SPOC deletion in human cells results in increased Pol II stalling, reduced elongation rate and an increase in mRNA stability, with marked derepression of neuronal genes. Key neuronal genes are aberrantly expressed in Phf3 knock-out mouse embryonic stem cells, resulting in impaired neuronal differentiation. Our data suggest that PHF3 acts as a prominent effector of neuronal gene regulation by bridging transcription with mRNA decay.","lang":"eng"}],"keyword":["general physics and astronomy","general biochemistry","genetics and molecular biology","general chemistry"],"citation":{"mla":"Appel, Lisa-Marie, et al. “PHF3 Regulates Neuronal Gene Expression through the Pol II CTD Reader Domain SPOC.” <i>Nature Communications</i>, vol. 12, no. 1, 6078, Springer Nature, 2021, doi:<a href=\"https://doi.org/10.1038/s41467-021-26360-2\">10.1038/s41467-021-26360-2</a>.","ieee":"L.-M. Appel <i>et al.</i>, “PHF3 regulates neuronal gene expression through the Pol II CTD reader domain SPOC,” <i>Nature Communications</i>, vol. 12, no. 1. Springer Nature, 2021.","chicago":"Appel, Lisa-Marie, Vedran Franke, Melania Bruno, Irina Grishkovskaya, Aiste Kasiliauskaite, Tanja Kaufmann, Ursula E. Schoeberl, et al. “PHF3 Regulates Neuronal Gene Expression through the Pol II CTD Reader Domain SPOC.” <i>Nature Communications</i>. Springer Nature, 2021. <a href=\"https://doi.org/10.1038/s41467-021-26360-2\">https://doi.org/10.1038/s41467-021-26360-2</a>.","apa":"Appel, L.-M., Franke, V., Bruno, M., Grishkovskaya, I., Kasiliauskaite, A., Kaufmann, T., … Slade, D. (2021). PHF3 regulates neuronal gene expression through the Pol II CTD reader domain SPOC. <i>Nature Communications</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41467-021-26360-2\">https://doi.org/10.1038/s41467-021-26360-2</a>","ista":"Appel L-M, Franke V, Bruno M, Grishkovskaya I, Kasiliauskaite A, Kaufmann T, Schoeberl UE, Puchinger MG, Kostrhon S, Ebenwaldner C, Sebesta M, Beltzung E, Mechtler K, Lin G, Vlasova A, Leeb M, Pavri R, Stark A, Akalin A, Stefl R, Bernecky C, Djinovic-Carugo K, Slade D. 2021. PHF3 regulates neuronal gene expression through the Pol II CTD reader domain SPOC. Nature Communications. 12(1), 6078.","ama":"Appel L-M, Franke V, Bruno M, et al. PHF3 regulates neuronal gene expression through the Pol II CTD reader domain SPOC. <i>Nature Communications</i>. 2021;12(1). doi:<a href=\"https://doi.org/10.1038/s41467-021-26360-2\">10.1038/s41467-021-26360-2</a>","short":"L.-M. Appel, V. Franke, M. Bruno, I. Grishkovskaya, A. Kasiliauskaite, T. Kaufmann, U.E. Schoeberl, M.G. Puchinger, S. Kostrhon, C. Ebenwaldner, M. Sebesta, E. Beltzung, K. Mechtler, G. Lin, A. Vlasova, M. Leeb, R. Pavri, A. Stark, A. Akalin, R. Stefl, C. Bernecky, K. Djinovic-Carugo, D. Slade, Nature Communications 12 (2021)."},"intvolume":"        12","day":"19","has_accepted_license":"1","language":[{"iso":"eng"}],"file_date_updated":"2021-10-21T13:51:49Z","type":"journal_article","doi":"10.1038/s41467-021-26360-2","publisher":"Springer Nature","issue":"1","publication":"Nature Communications","ddc":["610"],"author":[{"full_name":"Appel, Lisa-Marie","first_name":"Lisa-Marie","last_name":"Appel"},{"last_name":"Franke","first_name":"Vedran","full_name":"Franke, Vedran"},{"first_name":"Melania","full_name":"Bruno, Melania","last_name":"Bruno"},{"last_name":"Grishkovskaya","first_name":"Irina","full_name":"Grishkovskaya, Irina"},{"last_name":"Kasiliauskaite","first_name":"Aiste","full_name":"Kasiliauskaite, Aiste"},{"first_name":"Tanja","full_name":"Kaufmann, Tanja","last_name":"Kaufmann"},{"last_name":"Schoeberl","first_name":"Ursula E.","full_name":"Schoeberl, Ursula E."},{"first_name":"Martin G.","full_name":"Puchinger, Martin G.","last_name":"Puchinger"},{"last_name":"Kostrhon","first_name":"Sebastian","full_name":"Kostrhon, Sebastian"},{"full_name":"Ebenwaldner, Carmen","first_name":"Carmen","last_name":"Ebenwaldner"},{"last_name":"Sebesta","full_name":"Sebesta, Marek","first_name":"Marek"},{"first_name":"Etienne","full_name":"Beltzung, Etienne","last_name":"Beltzung"},{"full_name":"Mechtler, Karl","first_name":"Karl","last_name":"Mechtler"},{"full_name":"Lin, Gen","first_name":"Gen","last_name":"Lin"},{"last_name":"Vlasova","full_name":"Vlasova, Anna","first_name":"Anna"},{"last_name":"Leeb","first_name":"Martin","full_name":"Leeb, Martin"},{"first_name":"Rushad","full_name":"Pavri, Rushad","last_name":"Pavri"},{"first_name":"Alexander","full_name":"Stark, Alexander","last_name":"Stark"},{"last_name":"Akalin","first_name":"Altuna","full_name":"Akalin, Altuna"},{"first_name":"Richard","full_name":"Stefl, Richard","last_name":"Stefl"},{"orcid":"0000-0003-0893-7036","id":"2CB9DFE2-F248-11E8-B48F-1D18A9856A87","first_name":"Carrie A","full_name":"Bernecky, Carrie A","last_name":"Bernecky"},{"full_name":"Djinovic-Carugo, Kristina","first_name":"Kristina","last_name":"Djinovic-Carugo"},{"first_name":"Dea","full_name":"Slade, Dea","last_name":"Slade"}],"external_id":{"isi":["000709050300001"]},"oa_version":"Published Version","date_published":"2021-10-19T00:00:00Z","volume":12,"isi":1,"file":[{"file_name":"2021_NatComm_Appel.pdf","success":1,"date_updated":"2021-10-21T13:51:49Z","access_level":"open_access","date_created":"2021-10-21T13:51:49Z","file_id":"10169","content_type":"application/pdf","checksum":"d99fcd51aebde19c21314e3de0148007","relation":"main_file","creator":"cchlebak","file_size":5111706}],"quality_controlled":"1","date_created":"2021-10-20T14:40:32Z","year":"2021","date_updated":"2023-08-14T08:02:31Z","publication_identifier":{"eissn":["2041-1723"]},"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"article_processing_charge":"No"},{"quality_controlled":"1","page":"3798-3806","year":"2021","date_created":"2021-11-25T16:06:42Z","date_updated":"2021-11-30T08:20:09Z","volume":17,"article_processing_charge":"No","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by-nc/3.0/legalcode","name":"Creative Commons Attribution-NonCommercial 3.0 Unported (CC BY-NC 3.0)","image":"/images/cc_by_nc.png","short":"CC BY-NC (3.0)"},"publication_identifier":{"issn":["1744-683X"],"eissn":["1744-6848"]},"scopus_import":"1","pmid":1,"main_file_link":[{"open_access":"1","url":"https://pubs.rsc.org/en/content/articlehtml/2021/sm/d0sm02012e"}],"doi":"10.1039/d0sm02012e","publisher":"Royal Society of Chemistry","language":[{"iso":"eng"}],"type":"journal_article","license":"https://creativecommons.org/licenses/by-nc/3.0/","oa_version":"Published Version","external_id":{"pmid":["33629089"]},"date_published":"2021-02-16T00:00:00Z","issue":"14","publication":"Soft Matter","author":[{"last_name":"Vanhille-Campos","first_name":"Christian","full_name":"Vanhille-Campos, Christian"},{"orcid":"0000-0002-7854-2139","id":"bf63d406-f056-11eb-b41d-f263a6566d8b","last_name":"Šarić","full_name":"Šarić, Anđela","first_name":"Anđela"}],"status":"public","abstract":[{"text":"We study the effects of osmotic shocks on lipid vesicles via coarse-grained molecular dynamics simulations by explicitly considering the solute in the system. We find that depending on their nature (hypo- or hypertonic) such shocks can lead to bursting events or engulfing of external material into inner compartments, among other morphology transformations. We characterize the dynamics of these processes and observe a separation of time scales between the osmotic shock absorption and the shape relaxation. Our work consequently provides an insight into the dynamics of compartmentalization in vesicular systems as a result of osmotic shocks, which can be of interest in the context of early proto-cell development and proto-cell compartmentalisation.","lang":"eng"}],"extern":"1","day":"16","keyword":["condensed matter physics","general chemistry"],"intvolume":"        17","citation":{"short":"C. Vanhille-Campos, A. Šarić, Soft Matter 17 (2021) 3798–3806.","ama":"Vanhille-Campos C, Šarić A. Modelling the dynamics of vesicle reshaping and scission under osmotic shocks. <i>Soft Matter</i>. 2021;17(14):3798-3806. doi:<a href=\"https://doi.org/10.1039/d0sm02012e\">10.1039/d0sm02012e</a>","chicago":"Vanhille-Campos, Christian, and Anđela Šarić. “Modelling the Dynamics of Vesicle Reshaping and Scission under Osmotic Shocks.” <i>Soft Matter</i>. Royal Society of Chemistry, 2021. <a href=\"https://doi.org/10.1039/d0sm02012e\">https://doi.org/10.1039/d0sm02012e</a>.","ista":"Vanhille-Campos C, Šarić A. 2021. Modelling the dynamics of vesicle reshaping and scission under osmotic shocks. Soft Matter. 17(14), 3798–3806.","apa":"Vanhille-Campos, C., &#38; Šarić, A. (2021). Modelling the dynamics of vesicle reshaping and scission under osmotic shocks. <i>Soft Matter</i>. Royal Society of Chemistry. <a href=\"https://doi.org/10.1039/d0sm02012e\">https://doi.org/10.1039/d0sm02012e</a>","ieee":"C. Vanhille-Campos and A. Šarić, “Modelling the dynamics of vesicle reshaping and scission under osmotic shocks,” <i>Soft Matter</i>, vol. 17, no. 14. Royal Society of Chemistry, pp. 3798–3806, 2021.","mla":"Vanhille-Campos, Christian, and Anđela Šarić. “Modelling the Dynamics of Vesicle Reshaping and Scission under Osmotic Shocks.” <i>Soft Matter</i>, vol. 17, no. 14, Royal Society of Chemistry, 2021, pp. 3798–806, doi:<a href=\"https://doi.org/10.1039/d0sm02012e\">10.1039/d0sm02012e</a>."},"user_id":"8b945eb4-e2f2-11eb-945a-df72226e66a9","month":"02","_id":"10339","oa":1,"related_material":{"link":[{"relation":"earlier_version","url":"https://www.biorxiv.org/content/10.1101/2020.11.16.384602v2"}]},"title":"Modelling the dynamics of vesicle reshaping and scission under osmotic shocks","publication_status":"published","article_type":"original","acknowledgement":"We acknowledge support from the Royal Society (C. V. C. and A. Sˇ.), the Medical Research Council (C. V. C. and A. Sˇ.), and the European Research Council (Starting grant ‘‘NEPA’’ 802960 to A. Sˇ.). We thank Johannes Krausser and Ivan Palaia for fruitful discussions."},{"volume":12,"file":[{"file_size":6166295,"creator":"kschuh","relation":"main_file","checksum":"53ccc53d09a9111143839dbe7784e663","content_type":"application/pdf","file_id":"9538","date_created":"2021-06-09T15:21:14Z","access_level":"open_access","success":1,"date_updated":"2021-06-09T15:21:14Z","file_name":"2021_NatureCommunications_Obr.pdf"}],"isi":1,"quality_controlled":"1","year":"2021","date_created":"2021-05-28T14:25:50Z","date_updated":"2023-08-08T13:53:53Z","acknowledged_ssus":[{"_id":"ScienComp"},{"_id":"LifeSc"},{"_id":"EM-Fac"}],"project":[{"grant_number":"P31445","name":"Structural conservation and diversity in retroviral capsid","_id":"26736D6A-B435-11E9-9278-68D0E5697425","call_identifier":"FWF"}],"publication_identifier":{"eissn":["2041-1723"]},"scopus_import":"1","article_processing_charge":"No","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"language":[{"iso":"eng"}],"file_date_updated":"2021-06-09T15:21:14Z","type":"journal_article","doi":"10.1038/s41467-021-23506-0","publisher":"Nature Research","issue":"1","publication":"Nature Communications","ddc":["570"],"author":[{"id":"4741CA5A-F248-11E8-B48F-1D18A9856A87","last_name":"Obr","first_name":"Martin","full_name":"Obr, Martin"},{"last_name":"Ricana","full_name":"Ricana, Clifton L.","first_name":"Clifton L."},{"full_name":"Nikulin, Nadia","first_name":"Nadia","last_name":"Nikulin"},{"last_name":"Feathers","full_name":"Feathers, Jon-Philip R.","first_name":"Jon-Philip R."},{"last_name":"Klanschnig","full_name":"Klanschnig, Marco","first_name":"Marco"},{"last_name":"Thader","first_name":"Andreas","full_name":"Thader, Andreas","id":"3A18A7B8-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Johnson, Marc C.","first_name":"Marc C.","last_name":"Johnson"},{"full_name":"Vogt, Volker M.","first_name":"Volker M.","last_name":"Vogt"},{"first_name":"Florian KM","full_name":"Schur, Florian KM","last_name":"Schur","id":"48AD8942-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-4790-8078"},{"last_name":"Dick","full_name":"Dick, Robert A.","first_name":"Robert A."}],"oa_version":"Published Version","external_id":{"isi":["000659145000011"]},"date_published":"2021-05-28T00:00:00Z","article_number":"3226","status":"public","abstract":[{"text":"Inositol hexakisphosphate (IP6) is an assembly cofactor for HIV-1. We report here that IP6 is also used for assembly of Rous sarcoma virus (RSV), a retrovirus from a different genus. IP6 is ~100-fold more potent at promoting RSV mature capsid protein (CA) assembly than observed for HIV-1 and removal of IP6 in cells reduces infectivity by 100-fold. Here, visualized by cryo-electron tomography and subtomogram averaging, mature capsid-like particles show an IP6-like density in the CA hexamer, coordinated by rings of six lysines and six arginines. Phosphate and IP6 have opposing effects on CA in vitro assembly, inducing formation of T = 1 icosahedrons and tubes, respectively, implying that phosphate promotes pentamer and IP6 hexamer formation. Subtomogram averaging and classification optimized for analysis of pleomorphic retrovirus particles reveal that the heterogeneity of mature RSV CA polyhedrons results from an unexpected, intrinsic CA hexamer flexibility. In contrast, the CA pentamer forms rigid units organizing the local architecture. These different features of hexamers and pentamers determine the structural mechanism to form CA polyhedrons of variable shape in mature RSV particles.","lang":"eng"}],"keyword":["General Biochemistry","Genetics and Molecular Biology","General Physics and Astronomy","General Chemistry"],"intvolume":"        12","citation":{"short":"M. Obr, C.L. Ricana, N. Nikulin, J.-P.R. Feathers, M. Klanschnig, A. Thader, M.C. Johnson, V.M. Vogt, F.K. Schur, R.A. Dick, Nature Communications 12 (2021).","ama":"Obr M, Ricana CL, Nikulin N, et al. Structure of the mature Rous sarcoma virus lattice reveals a role for IP6 in the formation of the capsid hexamer. <i>Nature Communications</i>. 2021;12(1). doi:<a href=\"https://doi.org/10.1038/s41467-021-23506-0\">10.1038/s41467-021-23506-0</a>","apa":"Obr, M., Ricana, C. L., Nikulin, N., Feathers, J.-P. R., Klanschnig, M., Thader, A., … Dick, R. A. (2021). Structure of the mature Rous sarcoma virus lattice reveals a role for IP6 in the formation of the capsid hexamer. <i>Nature Communications</i>. Nature Research. <a href=\"https://doi.org/10.1038/s41467-021-23506-0\">https://doi.org/10.1038/s41467-021-23506-0</a>","ista":"Obr M, Ricana CL, Nikulin N, Feathers J-PR, Klanschnig M, Thader A, Johnson MC, Vogt VM, Schur FK, Dick RA. 2021. Structure of the mature Rous sarcoma virus lattice reveals a role for IP6 in the formation of the capsid hexamer. Nature Communications. 12(1), 3226.","chicago":"Obr, Martin, Clifton L. Ricana, Nadia Nikulin, Jon-Philip R. Feathers, Marco Klanschnig, Andreas Thader, Marc C. Johnson, Volker M. Vogt, Florian KM Schur, and Robert A. Dick. “Structure of the Mature Rous Sarcoma Virus Lattice Reveals a Role for IP6 in the Formation of the Capsid Hexamer.” <i>Nature Communications</i>. Nature Research, 2021. <a href=\"https://doi.org/10.1038/s41467-021-23506-0\">https://doi.org/10.1038/s41467-021-23506-0</a>.","mla":"Obr, Martin, et al. “Structure of the Mature Rous Sarcoma Virus Lattice Reveals a Role for IP6 in the Formation of the Capsid Hexamer.” <i>Nature Communications</i>, vol. 12, no. 1, 3226, Nature Research, 2021, doi:<a href=\"https://doi.org/10.1038/s41467-021-23506-0\">10.1038/s41467-021-23506-0</a>.","ieee":"M. Obr <i>et al.</i>, “Structure of the mature Rous sarcoma virus lattice reveals a role for IP6 in the formation of the capsid hexamer,” <i>Nature Communications</i>, vol. 12, no. 1. Nature Research, 2021."},"day":"28","has_accepted_license":"1","related_material":{"link":[{"relation":"press_release","url":"https://ist.ac.at/en/news/how-retroviruses-become-infectious/","description":"News on IST Homepage"}]},"title":"Structure of the mature Rous sarcoma virus lattice reveals a role for IP6 in the formation of the capsid hexamer","oa":1,"publication_status":"published","article_type":"original","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","_id":"9431","month":"05","acknowledgement":"This work was funded by the National Institute of Allergy and Infectious Diseases under awards R01AI147890 to R.A.D., R01AI150454 to V.M.V, R35GM136258 in support of J-P.R.F, and the Austrian Science Fund (FWF) grant P31445 to F.K.M.S. Access to high-resolution cryo-ET data acquisition at EMBL Heidelberg was supported by iNEXT (grant no. 653706), funded by the Horizon 2020 program of the European Union (PID 4246). We thank Wim Hagen and Felix Weis at EMBL Heidelberg for support in cryo-ET data acquisition. This work made use of the Cornell Center for Materials Research Shared Facilities, which are supported through the NSF MRSEC program (DMR-179875). This research was also supported by the Scientific Service Units (SSUs) of IST Austria through resources provided by Scientific Computing (SciComp), the Life Science Facility (LSF), and the Electron Microscopy Facility (EMF).","department":[{"_id":"FlSc"}]},{"file_date_updated":"2021-06-15T18:55:59Z","type":"journal_article","language":[{"iso":"eng"}],"publisher":"Springer Nature","doi":"10.1038/s41467-021-23854-x","ddc":["570"],"author":[{"first_name":"Michael","full_name":"Prattes, Michael","last_name":"Prattes"},{"full_name":"Grishkovskaya, Irina","first_name":"Irina","last_name":"Grishkovskaya"},{"full_name":"Hodirnau, Victor-Valentin","first_name":"Victor-Valentin","last_name":"Hodirnau","id":"3661B498-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Rössler","full_name":"Rössler, Ingrid","first_name":"Ingrid"},{"full_name":"Klein, Isabella","first_name":"Isabella","last_name":"Klein"},{"last_name":"Hetzmannseder","first_name":"Christina","full_name":"Hetzmannseder, Christina"},{"last_name":"Zisser","first_name":"Gertrude","full_name":"Zisser, Gertrude"},{"first_name":"Christian C.","full_name":"Gruber, Christian C.","last_name":"Gruber"},{"last_name":"Gruber","full_name":"Gruber, Karl","first_name":"Karl"},{"last_name":"Haselbach","full_name":"Haselbach, David","first_name":"David"},{"last_name":"Bergler","first_name":"Helmut","full_name":"Bergler, Helmut"}],"issue":"1","publication":"Nature Communications","date_published":"2021-06-09T00:00:00Z","oa_version":"Published Version","external_id":{"pmid":["34108481"],"isi":["000664874700014"]},"volume":12,"isi":1,"file":[{"creator":"cziletti","file_size":3397292,"checksum":"40fc24c1310930990b52a8ad1142ee97","relation":"main_file","date_updated":"2021-06-15T18:55:59Z","access_level":"open_access","success":1,"date_created":"2021-06-15T18:55:59Z","file_id":"9556","content_type":"application/pdf","file_name":"2021_NatureComm_Prattes.pdf"}],"date_updated":"2023-08-08T14:05:26Z","acknowledged_ssus":[{"_id":"EM-Fac"}],"quality_controlled":"1","date_created":"2021-06-10T14:57:45Z","year":"2021","pmid":1,"publication_identifier":{"eissn":["2041-1723"]},"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"article_processing_charge":"No","article_type":"original","title":"Structural basis for inhibition of the AAA-ATPase Drg1 by diazaborine","oa":1,"publication_status":"published","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","_id":"9540","month":"06","acknowledgement":"We are deeply grateful to the late Gregor Högenauer who built the foundation for this study with his visionary work on the inhibitor diazaborine and its bacterial target. We thank Rolf Breinbauer for insightful discussions on boron chemistry. We thank Anton Meinhart and Tim Clausen for the valuable discussion of the manuscript. We are indebted to Thomas Köcher for the MS measurement of the diazaborine-ATPγS adduct. We thank the team of the VBCF for support during early phases of this work and the IST Austria Electron Microscopy Facility for providing equipment. The lab of D.H. is supported by Boehringer Ingelheim. The work was funded by FWF projects P32536 and P32977 (to H.B.).","department":[{"_id":"EM-Fac"}],"abstract":[{"lang":"eng","text":"The hexameric AAA-ATPase Drg1 is a key factor in eukaryotic ribosome biogenesis and initiates cytoplasmic maturation of the large ribosomal subunit by releasing the shuttling maturation factor Rlp24. Drg1 monomers contain two AAA-domains (D1 and D2) that act in a concerted manner. Rlp24 release is inhibited by the drug diazaborine which blocks ATP hydrolysis in D2. The mode of inhibition was unknown. Here we show the first cryo-EM structure of Drg1 revealing the inhibitory mechanism. Diazaborine forms a covalent bond to the 2′-OH of the nucleotide in D2, explaining its specificity for this site. As a consequence, the D2 domain is locked in a rigid, inactive state, stalling the whole Drg1 hexamer. Resistance mechanisms identified include abolished drug binding and altered positioning of the nucleotide. Our results suggest nucleotide-modifying compounds as potential novel inhibitors for AAA-ATPases."}],"article_number":"3483","status":"public","intvolume":"        12","citation":{"mla":"Prattes, Michael, et al. “Structural Basis for Inhibition of the AAA-ATPase Drg1 by Diazaborine.” <i>Nature Communications</i>, vol. 12, no. 1, 3483, Springer Nature, 2021, doi:<a href=\"https://doi.org/10.1038/s41467-021-23854-x\">10.1038/s41467-021-23854-x</a>.","ieee":"M. Prattes <i>et al.</i>, “Structural basis for inhibition of the AAA-ATPase Drg1 by diazaborine,” <i>Nature Communications</i>, vol. 12, no. 1. Springer Nature, 2021.","ista":"Prattes M, Grishkovskaya I, Hodirnau V-V, Rössler I, Klein I, Hetzmannseder C, Zisser G, Gruber CC, Gruber K, Haselbach D, Bergler H. 2021. Structural basis for inhibition of the AAA-ATPase Drg1 by diazaborine. Nature Communications. 12(1), 3483.","apa":"Prattes, M., Grishkovskaya, I., Hodirnau, V.-V., Rössler, I., Klein, I., Hetzmannseder, C., … Bergler, H. (2021). Structural basis for inhibition of the AAA-ATPase Drg1 by diazaborine. <i>Nature Communications</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41467-021-23854-x\">https://doi.org/10.1038/s41467-021-23854-x</a>","chicago":"Prattes, Michael, Irina Grishkovskaya, Victor-Valentin Hodirnau, Ingrid Rössler, Isabella Klein, Christina Hetzmannseder, Gertrude Zisser, et al. “Structural Basis for Inhibition of the AAA-ATPase Drg1 by Diazaborine.” <i>Nature Communications</i>. Springer Nature, 2021. <a href=\"https://doi.org/10.1038/s41467-021-23854-x\">https://doi.org/10.1038/s41467-021-23854-x</a>.","ama":"Prattes M, Grishkovskaya I, Hodirnau V-V, et al. Structural basis for inhibition of the AAA-ATPase Drg1 by diazaborine. <i>Nature Communications</i>. 2021;12(1). doi:<a href=\"https://doi.org/10.1038/s41467-021-23854-x\">10.1038/s41467-021-23854-x</a>","short":"M. Prattes, I. Grishkovskaya, V.-V. Hodirnau, I. Rössler, I. Klein, C. Hetzmannseder, G. Zisser, C.C. Gruber, K. Gruber, D. Haselbach, H. Bergler, Nature Communications 12 (2021)."},"keyword":["General Biochemistry","Genetics and Molecular Biology","General Physics and Astronomy","General Chemistry"],"has_accepted_license":"1","day":"09"},{"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"article_processing_charge":"No","publication_identifier":{"issn":["2041-1723"]},"scopus_import":"1","year":"2021","date_created":"2021-08-06T07:22:55Z","quality_controlled":"1","project":[{"grant_number":"692692","name":"Biophysics and circuit function of a giant cortical glumatergic synapse","_id":"25B7EB9E-B435-11E9-9278-68D0E5697425","call_identifier":"H2020"},{"name":"The Wittgenstein Prize","grant_number":"Z00312","_id":"25C5A090-B435-11E9-9278-68D0E5697425","call_identifier":"FWF"}],"acknowledged_ssus":[{"_id":"SSU"}],"date_updated":"2023-08-10T14:16:16Z","file":[{"file_name":"2021_NatureCommunications_Vandael.pdf","date_updated":"2021-12-17T11:34:50Z","access_level":"open_access","success":1,"file_id":"10563","date_created":"2021-12-17T11:34:50Z","content_type":"application/pdf","checksum":"6036a8cdae95e1707c2a04d54e325ff4","relation":"main_file","creator":"kschuh","file_size":3108845}],"isi":1,"volume":12,"oa_version":"Published Version","external_id":{"isi":["000655481800014"]},"date_published":"2021-05-18T00:00:00Z","publication":"Nature Communications","issue":"1","author":[{"full_name":"Vandael, David H","first_name":"David H","last_name":"Vandael","orcid":"0000-0001-7577-1676","id":"3AE48E0A-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Yuji","full_name":"Okamoto, Yuji","last_name":"Okamoto","id":"3337E116-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-0408-6094"},{"id":"353C1B58-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-5001-4804","last_name":"Jonas","first_name":"Peter M","full_name":"Jonas, Peter M"}],"ddc":["570"],"doi":"10.1038/s41467-021-23153-5","publisher":"Springer","language":[{"iso":"eng"}],"type":"journal_article","file_date_updated":"2021-12-17T11:34:50Z","day":"18","has_accepted_license":"1","keyword":["general physics and astronomy","general biochemistry","genetics and molecular biology","general chemistry"],"intvolume":"        12","citation":{"ieee":"D. H. Vandael, Y. Okamoto, and P. M. Jonas, “Transsynaptic modulation of presynaptic short-term plasticity in hippocampal mossy fiber synapses,” <i>Nature Communications</i>, vol. 12, no. 1. Springer, 2021.","mla":"Vandael, David H., et al. “Transsynaptic Modulation of Presynaptic Short-Term Plasticity in Hippocampal Mossy Fiber Synapses.” <i>Nature Communications</i>, vol. 12, no. 1, 2912, Springer, 2021, doi:<a href=\"https://doi.org/10.1038/s41467-021-23153-5\">10.1038/s41467-021-23153-5</a>.","chicago":"Vandael, David H, Yuji Okamoto, and Peter M Jonas. “Transsynaptic Modulation of Presynaptic Short-Term Plasticity in Hippocampal Mossy Fiber Synapses.” <i>Nature Communications</i>. Springer, 2021. <a href=\"https://doi.org/10.1038/s41467-021-23153-5\">https://doi.org/10.1038/s41467-021-23153-5</a>.","apa":"Vandael, D. H., Okamoto, Y., &#38; Jonas, P. M. (2021). Transsynaptic modulation of presynaptic short-term plasticity in hippocampal mossy fiber synapses. <i>Nature Communications</i>. Springer. <a href=\"https://doi.org/10.1038/s41467-021-23153-5\">https://doi.org/10.1038/s41467-021-23153-5</a>","ista":"Vandael DH, Okamoto Y, Jonas PM. 2021. Transsynaptic modulation of presynaptic short-term plasticity in hippocampal mossy fiber synapses. Nature Communications. 12(1), 2912.","ama":"Vandael DH, Okamoto Y, Jonas PM. Transsynaptic modulation of presynaptic short-term plasticity in hippocampal mossy fiber synapses. <i>Nature Communications</i>. 2021;12(1). doi:<a href=\"https://doi.org/10.1038/s41467-021-23153-5\">10.1038/s41467-021-23153-5</a>","short":"D.H. Vandael, Y. Okamoto, P.M. Jonas, Nature Communications 12 (2021)."},"ec_funded":1,"status":"public","article_number":"2912","abstract":[{"lang":"eng","text":"The hippocampal mossy fiber synapse is a key synapse of the trisynaptic circuit. Post-tetanic potentiation (PTP) is the most powerful form of plasticity at this synaptic connection. It is widely believed that mossy fiber PTP is an entirely presynaptic phenomenon, implying that PTP induction is input-specific, and requires neither activity of multiple inputs nor stimulation of postsynaptic neurons. To directly test cooperativity and associativity, we made paired recordings between single mossy fiber terminals and postsynaptic CA3 pyramidal neurons in rat brain slices. By stimulating non-overlapping mossy fiber inputs converging onto single CA3 neurons, we confirm that PTP is input-specific and non-cooperative. Unexpectedly, mossy fiber PTP exhibits anti-associative induction properties. EPSCs show only minimal PTP after combined pre- and postsynaptic high-frequency stimulation with intact postsynaptic Ca2+ signaling, but marked PTP in the absence of postsynaptic spiking and after suppression of postsynaptic Ca2+ signaling (10 mM EGTA). PTP is largely recovered by inhibitors of voltage-gated R- and L-type Ca2+ channels, group II mGluRs, and vacuolar-type H+-ATPase, suggesting the involvement of retrograde vesicular glutamate signaling. Transsynaptic regulation of PTP extends the repertoire of synaptic computations, implementing a brake on mossy fiber detonation and a “smart teacher” function of hippocampal mossy fiber synapses."}],"acknowledgement":"We thank Drs. Carolina Borges-Merjane and Jose Guzman for critically reading the manuscript, and Pablo Castillo for discussions. We are grateful to Alois Schlögl for help with analysis, Florian Marr for excellent technical assistance and cell reconstruction, Christina Altmutter for technical help, Eleftheria Kralli-Beller for manuscript editing, and the Scientific Service Units of IST Austria for support. This project received funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation program (grant agreement No 692692) and the Fond zur Förderung der Wissenschaftlichen Forschung (Z 312-B27, Wittgenstein award), both to P.J.","department":[{"_id":"PeJo"}],"month":"05","_id":"9778","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","publication_status":"published","title":"Transsynaptic modulation of presynaptic short-term plasticity in hippocampal mossy fiber synapses","related_material":{"link":[{"description":"News on IST Homepage","url":"https://ist.ac.at/en/news/synaptic-transmission-not-a-one-way-street/","relation":"press_release"}]},"oa":1,"article_type":"original"},{"project":[{"call_identifier":"H2020","_id":"257EB838-B435-11E9-9278-68D0E5697425","name":"Hybrid Optomechanical Technologies","grant_number":"732894"},{"name":"A Fiber Optic Transceiver for Superconducting Qubits","grant_number":"758053","_id":"26336814-B435-11E9-9278-68D0E5697425","call_identifier":"H2020"},{"_id":"260C2330-B435-11E9-9278-68D0E5697425","name":"ISTplus - Postdoctoral Fellowships","grant_number":"754411","call_identifier":"H2020"},{"grant_number":"862644","name":"Quantum readout techniques and technologies","_id":"237CBA6C-32DE-11EA-91FC-C7463DDC885E","call_identifier":"H2020"},{"name":"Coherent on-chip conversion of superconducting qubit signals from microwaves to optical frequencies","_id":"2671EB66-B435-11E9-9278-68D0E5697425"}],"acknowledged_ssus":[{"_id":"NanoFab"}],"date_updated":"2024-08-07T07:11:51Z","date_created":"2020-09-18T10:56:20Z","year":"2020","quality_controlled":"1","isi":1,"file":[{"file_name":"2020_NatureComm_Arnold.pdf","content_type":"application/pdf","date_created":"2020-09-18T13:02:37Z","file_id":"8530","access_level":"open_access","success":1,"date_updated":"2020-09-18T13:02:37Z","relation":"main_file","checksum":"88f92544889eb18bb38e25629a422a86","file_size":1002818,"creator":"dernst"}],"volume":11,"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"article_processing_charge":"No","publication_identifier":{"issn":["2041-1723"]},"publisher":"Springer Nature","doi":"10.1038/s41467-020-18269-z","type":"journal_article","file_date_updated":"2020-09-18T13:02:37Z","language":[{"iso":"eng"}],"date_published":"2020-09-08T00:00:00Z","external_id":{"isi":["000577280200001"]},"oa_version":"Published Version","author":[{"first_name":"Georg M","full_name":"Arnold, Georg M","last_name":"Arnold","id":"3770C838-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-1397-7876"},{"first_name":"Matthias","full_name":"Wulf, Matthias","last_name":"Wulf","id":"45598606-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-6613-1378"},{"orcid":"0000-0003-0415-1423","id":"2D25E1F6-F248-11E8-B48F-1D18A9856A87","full_name":"Barzanjeh, Shabir","first_name":"Shabir","last_name":"Barzanjeh"},{"id":"2C21D6E8-F248-11E8-B48F-1D18A9856A87","first_name":"Elena","full_name":"Redchenko, Elena","last_name":"Redchenko"},{"orcid":"0000-0001-6249-5860","id":"3B82B0F8-F248-11E8-B48F-1D18A9856A87","last_name":"Rueda Sanchez","first_name":"Alfredo R","full_name":"Rueda Sanchez, Alfredo R"},{"orcid":"0000-0001-9868-2166","id":"29705398-F248-11E8-B48F-1D18A9856A87","full_name":"Hease, William J","first_name":"William J","last_name":"Hease"},{"first_name":"Farid","full_name":"Hassani, Farid","last_name":"Hassani","orcid":"0000-0001-6937-5773","id":"2AED110C-F248-11E8-B48F-1D18A9856A87"},{"id":"4B591CBA-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-8112-028X","full_name":"Fink, Johannes M","first_name":"Johannes M","last_name":"Fink"}],"ddc":["530"],"publication":"Nature Communications","ec_funded":1,"abstract":[{"text":"Practical quantum networks require low-loss and noise-resilient optical interconnects as well as non-Gaussian resources for entanglement distillation and distributed quantum computation. The latter could be provided by superconducting circuits but existing solutions to interface the microwave and optical domains lack either scalability or efficiency, and in most cases the conversion noise is not known. In this work we utilize the unique opportunities of silicon photonics, cavity optomechanics and superconducting circuits to demonstrate a fully integrated, coherent transducer interfacing the microwave X and the telecom S bands with a total (internal) bidirectional transduction efficiency of 1.2% (135%) at millikelvin temperatures. The coupling relies solely on the radiation pressure interaction mediated by the femtometer-scale motion of two silicon nanobeams reaching a <jats:italic>V</jats:italic><jats:sub><jats:italic>π</jats:italic></jats:sub> as low as 16 μV for sub-nanowatt pump powers. Without the associated optomechanical gain, we achieve a total (internal) pure conversion efficiency of up to 0.019% (1.6%), relevant for future noise-free operation on this qubit-compatible platform.","lang":"eng"}],"status":"public","article_number":"4460","has_accepted_license":"1","day":"08","intvolume":"        11","citation":{"ama":"Arnold GM, Wulf M, Barzanjeh S, et al. Converting microwave and telecom photons with a silicon photonic nanomechanical interface. <i>Nature Communications</i>. 2020;11. doi:<a href=\"https://doi.org/10.1038/s41467-020-18269-z\">10.1038/s41467-020-18269-z</a>","short":"G.M. Arnold, M. Wulf, S. Barzanjeh, E. Redchenko, A.R. Rueda Sanchez, W.J. Hease, F. Hassani, J.M. Fink, Nature Communications 11 (2020).","mla":"Arnold, Georg M., et al. “Converting Microwave and Telecom Photons with a Silicon Photonic Nanomechanical Interface.” <i>Nature Communications</i>, vol. 11, 4460, Springer Nature, 2020, doi:<a href=\"https://doi.org/10.1038/s41467-020-18269-z\">10.1038/s41467-020-18269-z</a>.","ieee":"G. M. Arnold <i>et al.</i>, “Converting microwave and telecom photons with a silicon photonic nanomechanical interface,” <i>Nature Communications</i>, vol. 11. Springer Nature, 2020.","ista":"Arnold GM, Wulf M, Barzanjeh S, Redchenko E, Rueda Sanchez AR, Hease WJ, Hassani F, Fink JM. 2020. Converting microwave and telecom photons with a silicon photonic nanomechanical interface. Nature Communications. 11, 4460.","chicago":"Arnold, Georg M, Matthias Wulf, Shabir Barzanjeh, Elena Redchenko, Alfredo R Rueda Sanchez, William J Hease, Farid Hassani, and Johannes M Fink. “Converting Microwave and Telecom Photons with a Silicon Photonic Nanomechanical Interface.” <i>Nature Communications</i>. Springer Nature, 2020. <a href=\"https://doi.org/10.1038/s41467-020-18269-z\">https://doi.org/10.1038/s41467-020-18269-z</a>.","apa":"Arnold, G. M., Wulf, M., Barzanjeh, S., Redchenko, E., Rueda Sanchez, A. R., Hease, W. J., … Fink, J. M. (2020). Converting microwave and telecom photons with a silicon photonic nanomechanical interface. <i>Nature Communications</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41467-020-18269-z\">https://doi.org/10.1038/s41467-020-18269-z</a>"},"keyword":["General Biochemistry","Genetics and Molecular Biology","General Physics and Astronomy","General Chemistry"],"_id":"8529","month":"09","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","article_type":"original","publication_status":"published","oa":1,"related_material":{"link":[{"url":"https://doi.org/10.1038/s41467-020-18912-9","relation":"erratum"},{"url":"https://ist.ac.at/en/news/how-to-transport-microwave-quantum-information-via-optical-fiber/","description":"News on IST Homepage","relation":"press_release"}],"record":[{"id":"13056","relation":"research_data","status":"public"}]},"title":"Converting microwave and telecom photons with a silicon photonic nanomechanical interface","department":[{"_id":"JoFi"}],"acknowledgement":"We thank Yuan Chen for performing supplementary FEM simulations and Andrew Higginbotham, Ralf Riedinger, Sungkun Hong, and Lorenzo Magrini for valuable discussions. This work was supported by IST Austria, the IST nanofabrication facility (NFF), the European Union’s Horizon 2020 research and innovation program under grant agreement no. 732894 (FET Proactive HOT) and the European Research Council under grant agreement no. 758053 (ERC StG QUNNECT). G.A. is the recipient of a DOC fellowship of the Austrian Academy of Sciences at IST Austria. W.H. is the recipient of an ISTplus postdoctoral fellowship with funding from the European Union’s Horizon 2020 research and innovation program under the Marie Sklodowska-Curie grant agreement no. 754411. J.M.F. acknowledges support from the Austrian Science Fund (FWF) through BeyondC (F71), a NOMIS foundation research grant, and the EU’s Horizon 2020 research and innovation program under grant agreement no. 862644 (FET Open QUARTET)."},{"status":"public","article_number":"4838","abstract":[{"lang":"eng","text":"Aqueous iodine based electrochemical energy storage is considered a potential candidate to improve sustainability and performance of current battery and supercapacitor technology. It harnesses the redox activity of iodide, iodine, and polyiodide species in the confined geometry of nanoporous carbon electrodes. However, current descriptions of the electrochemical reaction mechanism to interconvert these species are elusive. Here we show that electrochemical oxidation of iodide in nanoporous carbons forms persistent solid iodine deposits. Confinement slows down dissolution into triiodide and pentaiodide, responsible for otherwise significant self-discharge via shuttling. The main tools for these insights are in situ Raman spectroscopy and in situ small and wide-angle X-ray scattering (in situ SAXS/WAXS). In situ Raman confirms the reversible formation of triiodide and pentaiodide. In situ SAXS/WAXS indicates remarkable amounts of solid iodine deposited in the carbon nanopores. Combined with stochastic modeling, in situ SAXS allows quantifying the solid iodine volume fraction and visualizing the iodine structure on 3D lattice models at the sub-nanometer scale. Based on the derived mechanism, we demonstrate strategies for improved iodine pore filling capacity and prevention of self-discharge, applicable to hybrid supercapacitors and batteries."}],"day":"24","has_accepted_license":"1","keyword":["General Biochemistry","Genetics and Molecular Biology","General Physics and Astronomy","General Chemistry"],"intvolume":"        11","citation":{"ama":"Prehal C, Fitzek H, Kothleitner G, et al. Persistent and reversible solid iodine electrodeposition in nanoporous carbons. <i>Nature Communications</i>. 2020;11. doi:<a href=\"https://doi.org/10.1038/s41467-020-18610-6\">10.1038/s41467-020-18610-6</a>","short":"C. Prehal, H. Fitzek, G. Kothleitner, V. Presser, B. Gollas, S.A. Freunberger, Q. Abbas, Nature Communications 11 (2020).","ieee":"C. Prehal <i>et al.</i>, “Persistent and reversible solid iodine electrodeposition in nanoporous carbons,” <i>Nature Communications</i>, vol. 11. Springer Nature, 2020.","mla":"Prehal, Christian, et al. “Persistent and Reversible Solid Iodine Electrodeposition in Nanoporous Carbons.” <i>Nature Communications</i>, vol. 11, 4838, Springer Nature, 2020, doi:<a href=\"https://doi.org/10.1038/s41467-020-18610-6\">10.1038/s41467-020-18610-6</a>.","chicago":"Prehal, Christian, Harald Fitzek, Gerald Kothleitner, Volker Presser, Bernhard Gollas, Stefan Alexander Freunberger, and Qamar Abbas. “Persistent and Reversible Solid Iodine Electrodeposition in Nanoporous Carbons.” <i>Nature Communications</i>. Springer Nature, 2020. <a href=\"https://doi.org/10.1038/s41467-020-18610-6\">https://doi.org/10.1038/s41467-020-18610-6</a>.","ista":"Prehal C, Fitzek H, Kothleitner G, Presser V, Gollas B, Freunberger SA, Abbas Q. 2020. Persistent and reversible solid iodine electrodeposition in nanoporous carbons. Nature Communications. 11, 4838.","apa":"Prehal, C., Fitzek, H., Kothleitner, G., Presser, V., Gollas, B., Freunberger, S. A., &#38; Abbas, Q. (2020). Persistent and reversible solid iodine electrodeposition in nanoporous carbons. <i>Nature Communications</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41467-020-18610-6\">https://doi.org/10.1038/s41467-020-18610-6</a>"},"_id":"8568","month":"09","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","publication_status":"published","related_material":{"link":[{"relation":"erratum","url":"https://doi.org/10.1038/s41467-020-19720-x"}]},"oa":1,"title":"Persistent and reversible solid iodine electrodeposition in nanoporous carbons","article_type":"original","department":[{"_id":"StFr"}],"date_created":"2020-09-25T07:23:13Z","year":"2020","quality_controlled":"1","date_updated":"2023-08-22T09:37:24Z","isi":1,"file":[{"file_name":"2020_NatureComm_Prehal.pdf","content_type":"application/pdf","date_created":"2020-09-28T13:16:15Z","file_id":"8585","success":1,"access_level":"open_access","date_updated":"2020-09-28T13:16:15Z","relation":"main_file","checksum":"eada7bc8dd16a49390137cff882ef328","file_size":1822469,"creator":"dernst"}],"volume":11,"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"article_processing_charge":"No","publication_identifier":{"issn":["2041-1723"]},"doi":"10.1038/s41467-020-18610-6","publisher":"Springer Nature","language":[{"iso":"eng"}],"type":"journal_article","file_date_updated":"2020-09-28T13:16:15Z","external_id":{"isi":["000573756600004"]},"oa_version":"Published Version","date_published":"2020-09-24T00:00:00Z","publication":"Nature Communications","author":[{"last_name":"Prehal","first_name":"Christian","full_name":"Prehal, Christian"},{"last_name":"Fitzek","full_name":"Fitzek, Harald","first_name":"Harald"},{"last_name":"Kothleitner","full_name":"Kothleitner, Gerald","first_name":"Gerald"},{"full_name":"Presser, Volker","first_name":"Volker","last_name":"Presser"},{"last_name":"Gollas","first_name":"Bernhard","full_name":"Gollas, Bernhard"},{"orcid":"0000-0003-2902-5319","id":"A8CA28E6-CE23-11E9-AD2D-EC27E6697425","last_name":"Freunberger","first_name":"Stefan Alexander","full_name":"Freunberger, Stefan Alexander"},{"last_name":"Abbas","first_name":"Qamar","full_name":"Abbas, Qamar"}],"ddc":["530"]},{"publisher":"Springer Nature","doi":"10.1038/s41467-020-19372-x","type":"journal_article","file_date_updated":"2020-11-09T07:56:24Z","language":[{"iso":"eng"}],"date_published":"2020-11-04T00:00:00Z","external_id":{"isi":["000592028600001"]},"oa_version":"Published Version","author":[{"full_name":"Schulte, Linda","first_name":"Linda","last_name":"Schulte"},{"full_name":"Mao, Jiafei","first_name":"Jiafei","last_name":"Mao"},{"last_name":"Reitz","first_name":"Julian","full_name":"Reitz, Julian"},{"last_name":"Sreeramulu","first_name":"Sridhar","full_name":"Sreeramulu, Sridhar"},{"full_name":"Kudlinzki, Denis","first_name":"Denis","last_name":"Kudlinzki"},{"full_name":"Hodirnau, Victor-Valentin","first_name":"Victor-Valentin","last_name":"Hodirnau","id":"3661B498-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Meier-Credo, Jakob","first_name":"Jakob","last_name":"Meier-Credo"},{"full_name":"Saxena, Krishna","first_name":"Krishna","last_name":"Saxena"},{"full_name":"Buhr, Florian","first_name":"Florian","last_name":"Buhr"},{"full_name":"Langer, Julian D.","first_name":"Julian D.","last_name":"Langer"},{"last_name":"Blackledge","full_name":"Blackledge, Martin","first_name":"Martin"},{"last_name":"Frangakis","full_name":"Frangakis, Achilleas S.","first_name":"Achilleas S."},{"first_name":"Clemens","full_name":"Glaubitz, Clemens","last_name":"Glaubitz"},{"last_name":"Schwalbe","full_name":"Schwalbe, Harald","first_name":"Harald"}],"ddc":["570"],"publication":"Nature Communications","date_updated":"2023-08-22T12:36:07Z","year":"2020","date_created":"2020-11-09T07:49:36Z","quality_controlled":"1","file":[{"file_size":1670898,"creator":"dernst","relation":"main_file","checksum":"b2688f0347e69e6629bba582077278c5","file_id":"8745","content_type":"application/pdf","date_created":"2020-11-09T07:56:24Z","access_level":"open_access","date_updated":"2020-11-09T07:56:24Z","success":1,"file_name":"2020_NatureComm_Schulte.pdf"}],"isi":1,"volume":11,"article_processing_charge":"No","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"scopus_import":"1","publication_identifier":{"issn":["2041-1723"]},"month":"11","_id":"8744","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","article_type":"original","publication_status":"published","title":"Cysteine oxidation and disulfide formation in the ribosomal exit tunnel","oa":1,"acknowledgement":"We acknowledge help from Anja Seybert, Margot Frangakis, Diana Grewe, Mikhail Eltsov, Utz Ermel, and Shintaro Aibara. The work was supported by Deutsche Forschungsgemeinschaft in the CLiC graduate school. Work at the Center for Biomolecular Magnetic Resonance (BMRZ) is supported by the German state of Hesse. The work at BMRZ has been supported by the state of Hesse. L.S. has been supported by the DFG graduate college: CLiC.","department":[{"_id":"EM-Fac"}],"abstract":[{"text":"Understanding the conformational sampling of translation-arrested ribosome nascent chain complexes is key to understand co-translational folding. Up to now, coupling of cysteine oxidation, disulfide bond formation and structure formation in nascent chains has remained elusive. Here, we investigate the eye-lens protein γB-crystallin in the ribosomal exit tunnel. Using mass spectrometry, theoretical simulations, dynamic nuclear polarization-enhanced solid-state nuclear magnetic resonance and cryo-electron microscopy, we show that thiol groups of cysteine residues undergo S-glutathionylation and S-nitrosylation and form non-native disulfide bonds. Thus, covalent modification chemistry occurs already prior to nascent chain release as the ribosome exit tunnel provides sufficient space even for disulfide bond formation which can guide protein folding.","lang":"eng"}],"status":"public","article_number":"5569","has_accepted_license":"1","day":"04","citation":{"ieee":"L. Schulte <i>et al.</i>, “Cysteine oxidation and disulfide formation in the ribosomal exit tunnel,” <i>Nature Communications</i>, vol. 11. Springer Nature, 2020.","mla":"Schulte, Linda, et al. “Cysteine Oxidation and Disulfide Formation in the Ribosomal Exit Tunnel.” <i>Nature Communications</i>, vol. 11, 5569, Springer Nature, 2020, doi:<a href=\"https://doi.org/10.1038/s41467-020-19372-x\">10.1038/s41467-020-19372-x</a>.","ista":"Schulte L, Mao J, Reitz J, Sreeramulu S, Kudlinzki D, Hodirnau V-V, Meier-Credo J, Saxena K, Buhr F, Langer JD, Blackledge M, Frangakis AS, Glaubitz C, Schwalbe H. 2020. Cysteine oxidation and disulfide formation in the ribosomal exit tunnel. Nature Communications. 11, 5569.","apa":"Schulte, L., Mao, J., Reitz, J., Sreeramulu, S., Kudlinzki, D., Hodirnau, V.-V., … Schwalbe, H. (2020). Cysteine oxidation and disulfide formation in the ribosomal exit tunnel. <i>Nature Communications</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41467-020-19372-x\">https://doi.org/10.1038/s41467-020-19372-x</a>","chicago":"Schulte, Linda, Jiafei Mao, Julian Reitz, Sridhar Sreeramulu, Denis Kudlinzki, Victor-Valentin Hodirnau, Jakob Meier-Credo, et al. “Cysteine Oxidation and Disulfide Formation in the Ribosomal Exit Tunnel.” <i>Nature Communications</i>. Springer Nature, 2020. <a href=\"https://doi.org/10.1038/s41467-020-19372-x\">https://doi.org/10.1038/s41467-020-19372-x</a>.","ama":"Schulte L, Mao J, Reitz J, et al. Cysteine oxidation and disulfide formation in the ribosomal exit tunnel. <i>Nature Communications</i>. 2020;11. doi:<a href=\"https://doi.org/10.1038/s41467-020-19372-x\">10.1038/s41467-020-19372-x</a>","short":"L. Schulte, J. Mao, J. Reitz, S. Sreeramulu, D. Kudlinzki, V.-V. Hodirnau, J. Meier-Credo, K. Saxena, F. Buhr, J.D. Langer, M. Blackledge, A.S. Frangakis, C. Glaubitz, H. Schwalbe, Nature Communications 11 (2020)."},"intvolume":"        11","keyword":["General Biochemistry","Genetics and Molecular Biology","General Physics and Astronomy","General Chemistry"]},{"acknowledgement":"This research was supported by the Scientific Service Units (SSUs) of IST Austria through resources provided by Scientific Computing (SciComp), the Life Science Facility (LSF), the BioImaging Facility (BIF), and the Electron Microscopy Facility (EMF). We also thank Dimitry Tegunov (MPI for Biophysical Chemistry) for helpful discussions\r\nabout the M software, and Michael Sixt (IST Austria) and Klemens Rottner (Technical University Braunschweig, HZI Braunschweig) for critical reading of the manuscript. We also thank Gregory Voth (University of Chicago) for providing us the MD-derived branch junction model for comparison. The authors acknowledge support from IST Austria and from the Austrian Science Fund (FWF): M02495 to G.D. and Austrian Science Fund (FWF): P33367 to F.K.M.S. ","department":[{"_id":"FlSc"},{"_id":"EM-Fac"}],"related_material":{"link":[{"relation":"press_release","url":"https://ist.ac.at/en/news/cutting-edge-technology-reveals-structures-within-cells/","description":"News on IST Homepage"}]},"title":"Cryo-electron tomography structure of Arp2/3 complex in cells reveals new insights into the branch junction","oa":1,"publication_status":"published","article_type":"original","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","month":"12","_id":"8971","keyword":["General Biochemistry","Genetics and Molecular Biology","General Physics and Astronomy","General Chemistry"],"citation":{"mla":"Fäßler, Florian, et al. “Cryo-Electron Tomography Structure of Arp2/3 Complex in Cells Reveals New Insights into the Branch Junction.” <i>Nature Communications</i>, vol. 11, 6437, Springer Nature, 2020, doi:<a href=\"https://doi.org/10.1038/s41467-020-20286-x\">10.1038/s41467-020-20286-x</a>.","ieee":"F. Fäßler, G. A. Dimchev, V.-V. Hodirnau, W. Wan, and F. K. Schur, “Cryo-electron tomography structure of Arp2/3 complex in cells reveals new insights into the branch junction,” <i>Nature Communications</i>, vol. 11. Springer Nature, 2020.","ista":"Fäßler F, Dimchev GA, Hodirnau V-V, Wan W, Schur FK. 2020. Cryo-electron tomography structure of Arp2/3 complex in cells reveals new insights into the branch junction. Nature Communications. 11, 6437.","apa":"Fäßler, F., Dimchev, G. A., Hodirnau, V.-V., Wan, W., &#38; Schur, F. K. (2020). Cryo-electron tomography structure of Arp2/3 complex in cells reveals new insights into the branch junction. <i>Nature Communications</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41467-020-20286-x\">https://doi.org/10.1038/s41467-020-20286-x</a>","chicago":"Fäßler, Florian, Georgi A Dimchev, Victor-Valentin Hodirnau, William Wan, and Florian KM Schur. “Cryo-Electron Tomography Structure of Arp2/3 Complex in Cells Reveals New Insights into the Branch Junction.” <i>Nature Communications</i>. Springer Nature, 2020. <a href=\"https://doi.org/10.1038/s41467-020-20286-x\">https://doi.org/10.1038/s41467-020-20286-x</a>.","ama":"Fäßler F, Dimchev GA, Hodirnau V-V, Wan W, Schur FK. Cryo-electron tomography structure of Arp2/3 complex in cells reveals new insights into the branch junction. <i>Nature Communications</i>. 2020;11. doi:<a href=\"https://doi.org/10.1038/s41467-020-20286-x\">10.1038/s41467-020-20286-x</a>","short":"F. Fäßler, G.A. Dimchev, V.-V. Hodirnau, W. Wan, F.K. Schur, Nature Communications 11 (2020)."},"intvolume":"        11","day":"22","has_accepted_license":"1","article_number":"6437","status":"public","abstract":[{"lang":"eng","text":"The actin-related protein (Arp)2/3 complex nucleates branched actin filament networks pivotal for cell migration, endocytosis and pathogen infection. Its activation is tightly regulated and involves complex structural rearrangements and actin filament binding, which are yet to be understood. Here, we report a 9.0 Å resolution structure of the actin filament Arp2/3 complex branch junction in cells using cryo-electron tomography and subtomogram averaging. This allows us to generate an accurate model of the active Arp2/3 complex in the branch junction and its interaction with actin filaments. Notably, our model reveals a previously undescribed set of interactions of the Arp2/3 complex with the mother filament, significantly different to the previous branch junction model. Our structure also indicates a central role for the ArpC3 subunit in stabilizing the active conformation."}],"publication":"Nature Communications","ddc":["570"],"author":[{"last_name":"Fäßler","first_name":"Florian","full_name":"Fäßler, Florian","orcid":"0000-0001-7149-769X","id":"404F5528-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Georgi A","full_name":"Dimchev, Georgi A","last_name":"Dimchev","id":"38C393BE-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-8370-6161"},{"first_name":"Victor-Valentin","full_name":"Hodirnau, Victor-Valentin","last_name":"Hodirnau","id":"3661B498-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Wan","full_name":"Wan, William","first_name":"William"},{"last_name":"Schur","first_name":"Florian KM","full_name":"Schur, Florian KM","orcid":"0000-0003-4790-8078","id":"48AD8942-F248-11E8-B48F-1D18A9856A87"}],"oa_version":"Published Version","external_id":{"isi":["000603078000003"]},"date_published":"2020-12-22T00:00:00Z","language":[{"iso":"eng"}],"file_date_updated":"2020-12-28T08:16:10Z","type":"journal_article","doi":"10.1038/s41467-020-20286-x","publisher":"Springer Nature","publication_identifier":{"issn":["2041-1723"]},"scopus_import":"1","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"article_processing_charge":"No","volume":11,"isi":1,"file":[{"date_updated":"2020-12-28T08:16:10Z","access_level":"open_access","success":1,"content_type":"application/pdf","date_created":"2020-12-28T08:16:10Z","file_id":"8975","file_name":"2020_NatureComm_Faessler.pdf","creator":"dernst","file_size":3958727,"checksum":"55d43ea0061cc4027ba45e966e1db8cc","relation":"main_file"}],"quality_controlled":"1","year":"2020","date_created":"2020-12-23T08:25:45Z","date_updated":"2023-08-24T11:01:50Z","acknowledged_ssus":[{"_id":"ScienComp"},{"_id":"LifeSc"},{"_id":"Bio"},{"_id":"EM-Fac"}],"project":[{"_id":"9B954C5C-BA93-11EA-9121-9846C619BF3A","name":"Structure and isoform diversity of the Arp2/3 complex","grant_number":"P33367"},{"name":"Protein structure and function in filopodia across scales","grant_number":"M02495","_id":"2674F658-B435-11E9-9278-68D0E5697425","call_identifier":"FWF"}]}]