---
_id: '11879'
abstract:
- lang: eng
  text: "As the overall global mean surface temperature is increasing due to climate
    change, plant\r\nadaptation to those stressful conditions is of utmost importance
    for their survival. Plants are\r\nsessile organisms, thus to compensate for their
    lack of mobility, they evolved a variety of\r\nmechanisms enabling them to flexibly
    adjust their physiological, growth and developmental\r\nprocesses to fluctuating
    temperatures and to survive in harsh environments. While these unique\r\nadaptation
    abilities provide an important evolutionary advantage, overall modulation of plant\r\ngrowth
    and developmental program due to non-optimal temperature negatively affects biomass\r\nproduction,
    crop productivity or sensitivity to pathogens. Thus, understanding molecular\r\nprocesses
    underlying plant adaptation to increased temperature can provide important\r\nresources
    for breeding strategies to ensure sufficient agricultural food production.\r\nAn
    increase in ambient temperature by a few degrees leads to profound changes in
    organ growth\r\nincluding enhanced hypocotyl elongation, expansion of petioles,
    hyponastic growth of leaves and\r\ncotyledons, collectively named thermomorphogenesis
    (Casal & Balasubramanian, 2019). Auxin,\r\none of the best-studied growth hormones,
    plays an essential role in this process by direct\r\nactivation of transcriptional
    and non-transcriptional processes resulting in elongation growth\r\n(Majda & Robert,
    2018).To modulate hypocotyl growth in response to high ambient temperature\r\n(hAT),
    auxin needs to be redistributed accordingly. PINs, auxin efflux transporters,
    are key\r\ncomponents of the polar auxin transport (PAT) machinery, which controls
    the amount and\r\ndirection of auxin translocated in the plant tissues and organs(Adamowski
    & Friml, 2015). Hence,\r\nPIN-mediated transport is tightly linked with thermo-morphogenesis,
    and interference with PAT\r\nthrough either chemical or genetic means dramatically
    affecting the adaptive responses to hAT.\r\nIntriguingly, despite the key role
    of PIN mediated transport in growth response to hAT, whether\r\nand how PINs at
    the level of expression adapt to fluctuation in temperature is scarcely\r\nunderstood.\r\nWith
    genetic, molecular and advanced bio-imaging approaches, we demonstrate the role
    of PIN\r\nauxin transporters in the regulation of hypocotyl growth in response
    to hAT. We show that via\r\nadjustment of PIN3, PIN4 and PIN7 expression in cotyledons
    and hypocotyls, auxin distribution is modulated thereby determining elongation
    pattern of epidermal cells at hAT. Furthermore, we\r\nidentified three Zinc-Finger
    (ZF) transcription factors as novel molecular components of the\r\nthermo-regulatory
    network, which through negative regulation of PIN transcription adjust the\r\ntransport
    of auxin at hAT. Our results suggest that the ZF-PIN module might be a part of
    the\r\nnegative feedback loop attenuating the activity of the thermo-sensing pathway
    to restrain\r\nexaggerated growth and developmental responses to hAT."
acknowledged_ssus:
- _id: Bio
- _id: LifeSc
- _id: SSU
acknowledgement: I would like to acknowledge ISTA and all the people from the Scientific
  Service Units and at ISTA, in particular Dorota Jaworska for excellent technical
  and scientific support as well as ÖAW for funding my research for over 3 years (DOC
  ÖAW Fellowship PR1022OEAW02).
alternative_title:
- ISTA Thesis
article_processing_charge: No
author:
- first_name: Christina
  full_name: Artner, Christina
  id: 45DF286A-F248-11E8-B48F-1D18A9856A87
  last_name: Artner
citation:
  ama: Artner C. Modulation of auxin transport via ZF proteins adjust plant response
    to high ambient temperature. 2022. doi:<a href="https://doi.org/10.15479/at:ista:11879">10.15479/at:ista:11879</a>
  apa: Artner, C. (2022). <i>Modulation of auxin transport via ZF proteins adjust
    plant response to high ambient temperature</i>. Institute of Science and Technology
    Austria. <a href="https://doi.org/10.15479/at:ista:11879">https://doi.org/10.15479/at:ista:11879</a>
  chicago: Artner, Christina. “Modulation of Auxin Transport via ZF Proteins Adjust
    Plant Response to High Ambient Temperature.” Institute of Science and Technology
    Austria, 2022. <a href="https://doi.org/10.15479/at:ista:11879">https://doi.org/10.15479/at:ista:11879</a>.
  ieee: C. Artner, “Modulation of auxin transport via ZF proteins adjust plant response
    to high ambient temperature,” Institute of Science and Technology Austria, 2022.
  ista: Artner C. 2022. Modulation of auxin transport via ZF proteins adjust plant
    response to high ambient temperature. Institute of Science and Technology Austria.
  mla: Artner, Christina. <i>Modulation of Auxin Transport via ZF Proteins Adjust
    Plant Response to High Ambient Temperature</i>. Institute of Science and Technology
    Austria, 2022, doi:<a href="https://doi.org/10.15479/at:ista:11879">10.15479/at:ista:11879</a>.
  short: C. Artner, Modulation of Auxin Transport via ZF Proteins Adjust Plant Response
    to High Ambient Temperature, Institute of Science and Technology Austria, 2022.
date_created: 2022-08-17T07:58:53Z
date_published: 2022-08-17T00:00:00Z
date_updated: 2023-09-09T22:30:04Z
day: '17'
ddc:
- '580'
degree_awarded: PhD
department:
- _id: GradSch
- _id: EvBe
doi: 10.15479/at:ista:11879
file:
- access_level: open_access
  checksum: a2c2fdc28002538840490bfa6a08b2cb
  content_type: application/pdf
  creator: cartner
  date_created: 2022-08-17T12:08:49Z
  date_updated: 2023-09-09T22:30:03Z
  embargo: 2023-09-08
  file_id: '11907'
  file_name: ChristinaArtner_PhD_Thesis_2022.pdf
  file_size: 11113608
  relation: main_file
- access_level: closed
  checksum: 66b461c074b815fbe63481b3f46a9f43
  content_type: application/octet-stream
  creator: cartner
  date_created: 2022-08-17T12:08:59Z
  date_updated: 2023-09-09T22:30:03Z
  embargo_to: open_access
  file_id: '11908'
  file_name: ChristinaArtner_PhD_Thesis_2022.7z
  file_size: 19097730
  relation: source_file
file_date_updated: 2023-09-09T22:30:03Z
has_accepted_license: '1'
keyword:
- high ambient temperature
- auxin
- PINs
- Zinc-Finger proteins
- thermomorphogenesis
- stress
language:
- iso: eng
month: '08'
oa: 1
oa_version: Published Version
page: '128'
project:
- _id: 2685A872-B435-11E9-9278-68D0E5697425
  name: Hormonal regulation of plant adaptive responses to environmental signals
publication_identifier:
  isbn:
  - 978-3-99078-022-0
  issn:
  - 2663-337X
publication_status: published
publisher: Institute of Science and Technology Austria
status: public
supervisor:
- first_name: Eva
  full_name: Benková, Eva
  id: 38F4F166-F248-11E8-B48F-1D18A9856A87
  last_name: Benková
  orcid: 0000-0002-8510-9739
title: Modulation of auxin transport via ZF proteins adjust plant response to high
  ambient temperature
type: dissertation
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '2022'
...
---
_id: '10015'
abstract:
- lang: eng
  text: "Auxin plays a dual role in growth regulation and, depending on the tissue
    and concentration of the hormone, it can either promote or inhibit division and
    expansion processes in plants. Recent studies have revealed that, beyond transcriptional
    reprogramming, alternative auxincontrolled mechanisms regulate root growth. Here,
    we explored the impact of different concentrations of the synthetic auxin NAA
    that establish growth-promoting and -repressing conditions on the root tip proteome
    and phosphoproteome, generating a unique resource. From the phosphoproteome data,
    we pinpointed (novel) growth regulators, such as the RALF34-THE1 module. Our results,
    together with previously published studies, suggest that auxin, H+-ATPases, cell
    wall modifications and cell wall sensing receptor-like kinases are tightly embedded
    in a pathway regulating cell elongation. Furthermore, our study assigned a novel
    role to MKK2 as a regulator of primary root growth and a (potential) regulator
    of auxin biosynthesis and signalling, and suggests the importance of the MKK2\r\nThr31
    phosphorylation site for growth regulation in the Arabidopsis root tip."
acknowledgement: We thank the Nottingham Stock Centre for seeds, Frank Van Breusegem
  for the phb3 mutant, and Herman Höfte for the the1 mutant. Open Access Funding by
  the Austrian Science Fund (FWF).
alternative_title:
- Protein Phosphorylation and Cell Signaling in Plants
article_number: '1665 '
article_processing_charge: Yes
article_type: original
author:
- first_name: N
  full_name: Nikonorova, N
  last_name: Nikonorova
- first_name: E
  full_name: Murphy, E
  last_name: Murphy
- first_name: CF
  full_name: Fonseca de Lima, CF
  last_name: Fonseca de Lima
- first_name: S
  full_name: Zhu, S
  last_name: Zhu
- first_name: B
  full_name: van de Cotte, B
  last_name: van de Cotte
- first_name: LD
  full_name: Vu, LD
  last_name: Vu
- first_name: D
  full_name: Balcerowicz, D
  last_name: Balcerowicz
- first_name: Lanxin
  full_name: Li, Lanxin
  id: 367EF8FA-F248-11E8-B48F-1D18A9856A87
  last_name: Li
  orcid: 0000-0002-5607-272X
- first_name: X
  full_name: Kong, X
  last_name: Kong
- first_name: G
  full_name: De Rop, G
  last_name: De Rop
- first_name: T
  full_name: Beeckman, T
  last_name: Beeckman
- first_name: Jiří
  full_name: Friml, Jiří
  id: 4159519E-F248-11E8-B48F-1D18A9856A87
  last_name: Friml
  orcid: 0000-0002-8302-7596
- first_name: K
  full_name: Vissenberg, K
  last_name: Vissenberg
- first_name: PC
  full_name: Morris, PC
  last_name: Morris
- first_name: Z
  full_name: Ding, Z
  last_name: Ding
- first_name: I
  full_name: De Smet, I
  last_name: De Smet
citation:
  ama: Nikonorova N, Murphy E, Fonseca de Lima C, et al. The Arabidopsis root tip
    (phospho)proteomes at growth-promoting versus growth-repressing conditions reveal
    novel root growth regulators. <i>Cells</i>. 2021;10. doi:<a href="https://doi.org/10.3390/cells10071665">10.3390/cells10071665</a>
  apa: Nikonorova, N., Murphy, E., Fonseca de Lima, C., Zhu, S., van de Cotte, B.,
    Vu, L., … De Smet, I. (2021). The Arabidopsis root tip (phospho)proteomes at growth-promoting
    versus growth-repressing conditions reveal novel root growth regulators. <i>Cells</i>.
    MDPI. <a href="https://doi.org/10.3390/cells10071665">https://doi.org/10.3390/cells10071665</a>
  chicago: Nikonorova, N, E Murphy, CF Fonseca de Lima, S Zhu, B van de Cotte, LD
    Vu, D Balcerowicz, et al. “The Arabidopsis Root Tip (Phospho)Proteomes at Growth-Promoting
    versus Growth-Repressing Conditions Reveal Novel Root Growth Regulators.” <i>Cells</i>.
    MDPI, 2021. <a href="https://doi.org/10.3390/cells10071665">https://doi.org/10.3390/cells10071665</a>.
  ieee: N. Nikonorova <i>et al.</i>, “The Arabidopsis root tip (phospho)proteomes
    at growth-promoting versus growth-repressing conditions reveal novel root growth
    regulators,” <i>Cells</i>, vol. 10. MDPI, 2021.
  ista: Nikonorova N, Murphy E, Fonseca de Lima C, Zhu S, van de Cotte B, Vu L, Balcerowicz
    D, Li L, Kong X, De Rop G, Beeckman T, Friml J, Vissenberg K, Morris P, Ding Z,
    De Smet I. 2021. The Arabidopsis root tip (phospho)proteomes at growth-promoting
    versus growth-repressing conditions reveal novel root growth regulators. Cells.
    10, 1665.
  mla: Nikonorova, N., et al. “The Arabidopsis Root Tip (Phospho)Proteomes at Growth-Promoting
    versus Growth-Repressing Conditions Reveal Novel Root Growth Regulators.” <i>Cells</i>,
    vol. 10, 1665, MDPI, 2021, doi:<a href="https://doi.org/10.3390/cells10071665">10.3390/cells10071665</a>.
  short: N. Nikonorova, E. Murphy, C. Fonseca de Lima, S. Zhu, B. van de Cotte, L.
    Vu, D. Balcerowicz, L. Li, X. Kong, G. De Rop, T. Beeckman, J. Friml, K. Vissenberg,
    P. Morris, Z. Ding, I. De Smet, Cells 10 (2021).
date_created: 2021-09-14T11:36:20Z
date_published: 2021-07-02T00:00:00Z
date_updated: 2024-10-29T10:22:44Z
day: '02'
ddc:
- '575'
department:
- _id: JiFr
doi: 10.3390/cells10071665
ec_funded: 1
external_id:
  isi:
  - '000676604700001'
  pmid:
  - '34359847'
file:
- access_level: open_access
  checksum: 2a9f534b9c2200e72e2cde95afaf4eed
  content_type: application/pdf
  creator: cchlebak
  date_created: 2021-09-16T09:07:06Z
  date_updated: 2021-09-16T09:07:06Z
  file_id: '10021'
  file_name: 2021_Cells_Nikonorova.pdf
  file_size: 2667848
  relation: main_file
  success: 1
file_date_updated: 2021-09-16T09:07:06Z
has_accepted_license: '1'
intvolume: '        10'
isi: 1
keyword:
- primary root
- (phospho)proteomics
- auxin
- (receptor) kinase
language:
- iso: eng
month: '07'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 2564DBCA-B435-11E9-9278-68D0E5697425
  call_identifier: H2020
  grant_number: '665385'
  name: International IST Doctoral Program
- _id: 3AC91DDA-15DF-11EA-824D-93A3E7B544D1
  call_identifier: FWF
  name: FWF Open Access Fund
publication: Cells
publication_identifier:
  issn:
  - 2073-4409
publication_status: published
publisher: MDPI
quality_controlled: '1'
related_material:
  record:
  - id: '10083'
    relation: dissertation_contains
    status: public
status: public
title: The Arabidopsis root tip (phospho)proteomes at growth-promoting versus growth-repressing
  conditions reveal novel root growth regulators
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 10
year: '2021'
...
---
_id: '9986'
abstract:
- lang: eng
  text: Size control is a fundamental question in biology, showing incremental complexity
    in plants, whose cells possess a rigid cell wall. The phytohormone auxin is a
    vital growth regulator with central importance for differential growth control.
    Our results indicate that auxin-reliant growth programs affect the molecular complexity
    of xyloglucans, the major type of cell wall hemicellulose in eudicots. Auxin-dependent
    induction and repression of growth coincide with reduced and enhanced molecular
    complexity of xyloglucans, respectively. In agreement with a proposed function
    in growth control, genetic interference with xyloglucan side decorations distinctly
    modulates auxin-dependent differential growth rates. Our work proposes that auxin-dependent
    growth programs have a spatially defined effect on xyloglucan’s molecular structure,
    which in turn affects cell wall mechanics and specifies differential, gravitropic
    hypocotyl growth.
acknowledgement: "We are grateful to Paul Knox, Markus Pauly, Malcom O’Neill, and
  Ignacio Zarra for providing published material; the BOKU-VIBT Imaging Center for
  access and M. Debreczeny for expertise; J.I. Thaker and Georg Seifert for critical
  reading.\r\n"
article_number: '9222'
article_processing_charge: Yes
article_type: original
author:
- first_name: Silvia Melina
  full_name: Velasquez, Silvia Melina
  last_name: Velasquez
- first_name: Xiaoyuan
  full_name: Guo, Xiaoyuan
  last_name: Guo
- first_name: Marçal
  full_name: Gallemi, Marçal
  id: 460C6802-F248-11E8-B48F-1D18A9856A87
  last_name: Gallemi
  orcid: 0000-0003-4675-6893
- first_name: Bibek
  full_name: Aryal, Bibek
  last_name: Aryal
- first_name: Peter
  full_name: Venhuizen, Peter
  last_name: Venhuizen
- first_name: Elke
  full_name: Barbez, Elke
  last_name: Barbez
- first_name: Kai Alexander
  full_name: Dünser, Kai Alexander
  last_name: Dünser
- first_name: Martin
  full_name: Darino, Martin
  last_name: Darino
- first_name: Aleš
  full_name: Pӗnčík, Aleš
  last_name: Pӗnčík
- first_name: Ondřej
  full_name: Novák, Ondřej
  last_name: Novák
- first_name: Maria
  full_name: Kalyna, Maria
  last_name: Kalyna
- first_name: Gregory
  full_name: Mouille, Gregory
  last_name: Mouille
- first_name: Eva
  full_name: Benková, Eva
  id: 38F4F166-F248-11E8-B48F-1D18A9856A87
  last_name: Benková
  orcid: 0000-0002-8510-9739
- first_name: Rishikesh P.
  full_name: Bhalerao, Rishikesh P.
  last_name: Bhalerao
- first_name: Jozef
  full_name: Mravec, Jozef
  last_name: Mravec
- first_name: Jürgen
  full_name: Kleine-Vehn, Jürgen
  last_name: Kleine-Vehn
citation:
  ama: Velasquez SM, Guo X, Gallemi M, et al. Xyloglucan remodeling defines auxin-dependent
    differential tissue expansion in plants. <i>International Journal of Molecular
    Sciences</i>. 2021;22(17). doi:<a href="https://doi.org/10.3390/ijms22179222">10.3390/ijms22179222</a>
  apa: Velasquez, S. M., Guo, X., Gallemi, M., Aryal, B., Venhuizen, P., Barbez, E.,
    … Kleine-Vehn, J. (2021). Xyloglucan remodeling defines auxin-dependent differential
    tissue expansion in plants. <i>International Journal of Molecular Sciences</i>.
    MDPI. <a href="https://doi.org/10.3390/ijms22179222">https://doi.org/10.3390/ijms22179222</a>
  chicago: Velasquez, Silvia Melina, Xiaoyuan Guo, Marçal Gallemi, Bibek Aryal, Peter
    Venhuizen, Elke Barbez, Kai Alexander Dünser, et al. “Xyloglucan Remodeling Defines
    Auxin-Dependent Differential Tissue Expansion in Plants.” <i>International Journal
    of Molecular Sciences</i>. MDPI, 2021. <a href="https://doi.org/10.3390/ijms22179222">https://doi.org/10.3390/ijms22179222</a>.
  ieee: S. M. Velasquez <i>et al.</i>, “Xyloglucan remodeling defines auxin-dependent
    differential tissue expansion in plants,” <i>International Journal of Molecular
    Sciences</i>, vol. 22, no. 17. MDPI, 2021.
  ista: Velasquez SM, Guo X, Gallemi M, Aryal B, Venhuizen P, Barbez E, Dünser KA,
    Darino M, Pӗnčík A, Novák O, Kalyna M, Mouille G, Benková E, Bhalerao RP, Mravec
    J, Kleine-Vehn J. 2021. Xyloglucan remodeling defines auxin-dependent differential
    tissue expansion in plants. International Journal of Molecular Sciences. 22(17),
    9222.
  mla: Velasquez, Silvia Melina, et al. “Xyloglucan Remodeling Defines Auxin-Dependent
    Differential Tissue Expansion in Plants.” <i>International Journal of Molecular
    Sciences</i>, vol. 22, no. 17, 9222, MDPI, 2021, doi:<a href="https://doi.org/10.3390/ijms22179222">10.3390/ijms22179222</a>.
  short: S.M. Velasquez, X. Guo, M. Gallemi, B. Aryal, P. Venhuizen, E. Barbez, K.A.
    Dünser, M. Darino, A. Pӗnčík, O. Novák, M. Kalyna, G. Mouille, E. Benková, R.P.
    Bhalerao, J. Mravec, J. Kleine-Vehn, International Journal of Molecular Sciences
    22 (2021).
date_created: 2021-09-05T22:01:24Z
date_published: 2021-08-26T00:00:00Z
date_updated: 2023-10-31T19:29:38Z
day: '26'
ddc:
- '575'
department:
- _id: EvBe
doi: 10.3390/ijms22179222
external_id:
  isi:
  - '000694347100001'
  pmid:
  - '34502129'
file:
- access_level: open_access
  checksum: 6b7055cf89f1b7ed8594c3fdf56f000b
  content_type: application/pdf
  creator: cchlebak
  date_created: 2021-09-06T12:50:19Z
  date_updated: 2021-09-07T09:04:53Z
  file_id: '9988'
  file_name: 2021_IntJMolecularSciences_Velasquez.pdf
  file_size: 2162247
  relation: main_file
file_date_updated: 2021-09-07T09:04:53Z
has_accepted_license: '1'
intvolume: '        22'
isi: 1
issue: '17'
keyword:
- auxin
- growth
- cell wall
- xyloglucans
- hypocotyls
- gravitropism
language:
- iso: eng
month: '08'
oa: 1
oa_version: Published Version
pmid: 1
publication: International Journal of Molecular Sciences
publication_identifier:
  eissn:
  - 1422-0067
  issn:
  - 1661-6596
publication_status: published
publisher: MDPI
quality_controlled: '1'
scopus_import: '1'
status: public
title: Xyloglucan remodeling defines auxin-dependent differential tissue expansion
  in plants
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 22
year: '2021'
...