[{"intvolume":"         8","status":"public","day":"20","type":"journal_article","issue":"18","publication":"Bio-Protocol","file_date_updated":"2020-07-14T12:47:28Z","publisher":"Bio-Protocol","language":[{"iso":"eng"}],"month":"09","date_published":"2018-09-20T00:00:00Z","date_created":"2019-04-29T09:40:33Z","file":[{"date_created":"2019-04-30T08:04:33Z","checksum":"d4588377e789da7f360b553ae02c5119","file_name":"2018_BioProtocol_Fan.pdf","file_size":2928337,"date_updated":"2020-07-14T12:47:28Z","access_level":"open_access","creator":"dernst","file_id":"6360","content_type":"application/pdf","relation":"main_file"}],"license":"https://creativecommons.org/licenses/by/4.0/","has_accepted_license":"1","department":[{"_id":"MiSi"}],"abstract":[{"text":"Blood platelets are critical for hemostasis and thrombosis, but also play diverse roles during immune responses. We have recently reported that platelets migrate at sites of infection in vitro and in vivo. Importantly, platelets use their ability to migrate to collect and bundle fibrin (ogen)-bound bacteria accomplishing efficient intravascular bacterial trapping. Here, we describe a method that allows analyzing platelet migration in vitro, focusing on their ability to collect bacteria and trap bacteria under flow.","lang":"eng"}],"author":[{"full_name":"Fan, Shuxia","last_name":"Fan","first_name":"Shuxia"},{"first_name":"Michael","last_name":"Lorenz","full_name":"Lorenz, Michael"},{"first_name":"Steffen","last_name":"Massberg","full_name":"Massberg, Steffen"},{"last_name":"Gärtner","full_name":"Gärtner, Florian R","orcid":"0000-0001-6120-3723","first_name":"Florian R","id":"397A88EE-F248-11E8-B48F-1D18A9856A87"}],"keyword":["Platelets","Cell migration","Bacteria","Shear flow","Fibrinogen","E. coli"],"publication_status":"published","citation":{"chicago":"Fan, Shuxia, Michael Lorenz, Steffen Massberg, and Florian R Gärtner. “Platelet Migration and Bacterial Trapping Assay under Flow.” <i>Bio-Protocol</i>. Bio-Protocol, 2018. <a href=\"https://doi.org/10.21769/bioprotoc.3018\">https://doi.org/10.21769/bioprotoc.3018</a>.","ieee":"S. Fan, M. Lorenz, S. Massberg, and F. R. Gärtner, “Platelet migration and bacterial trapping assay under flow,” <i>Bio-Protocol</i>, vol. 8, no. 18. Bio-Protocol, 2018.","apa":"Fan, S., Lorenz, M., Massberg, S., &#38; Gärtner, F. R. (2018). Platelet migration and bacterial trapping assay under flow. <i>Bio-Protocol</i>. Bio-Protocol. <a href=\"https://doi.org/10.21769/bioprotoc.3018\">https://doi.org/10.21769/bioprotoc.3018</a>","ista":"Fan S, Lorenz M, Massberg S, Gärtner FR. 2018. Platelet migration and bacterial trapping assay under flow. Bio-Protocol. 8(18), e3018.","short":"S. Fan, M. Lorenz, S. Massberg, F.R. Gärtner, Bio-Protocol 8 (2018).","mla":"Fan, Shuxia, et al. “Platelet Migration and Bacterial Trapping Assay under Flow.” <i>Bio-Protocol</i>, vol. 8, no. 18, e3018, Bio-Protocol, 2018, doi:<a href=\"https://doi.org/10.21769/bioprotoc.3018\">10.21769/bioprotoc.3018</a>.","ama":"Fan S, Lorenz M, Massberg S, Gärtner FR. Platelet migration and bacterial trapping assay under flow. <i>Bio-Protocol</i>. 2018;8(18). doi:<a href=\"https://doi.org/10.21769/bioprotoc.3018\">10.21769/bioprotoc.3018</a>"},"_id":"6354","publication_identifier":{"issn":["2331-8325"]},"acknowledgement":" FöFoLe project 947 (F.G.), the Friedrich-Baur-Stiftung project 41/16 (F.G.)","user_id":"3E5EF7F0-F248-11E8-B48F-1D18A9856A87","project":[{"grant_number":"747687","name":"Mechanical Adaptation of Lamellipodial Actin Networks in Migrating Cells","_id":"260AA4E2-B435-11E9-9278-68D0E5697425","call_identifier":"H2020"}],"quality_controlled":"1","oa_version":"Published Version","oa":1,"date_updated":"2021-01-12T08:07:12Z","volume":8,"title":"Platelet migration and bacterial trapping assay under flow","year":"2018","doi":"10.21769/bioprotoc.3018","ec_funded":1,"ddc":["570"],"tmp":{"image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"article_number":"e3018"}]