---
_id: '14776'
abstract:
- lang: eng
  text: Soluble chaperones residing in the endoplasmic reticulum (ER) play vitally
    important roles in folding and quality control of newly synthesized proteins that
    transiently pass through the ER en route to their final destinations. These soluble
    residents of the ER are themselves endowed with an ER retrieval signal that enables
    the cell to bring the escaped residents back from the Golgi. Here, by using purified
    proteins, we showed that Nicotiana tabacum phytaspase, a plant aspartate-specific
    protease, introduces two breaks at the C-terminus of the N. tabacum ER resident
    calreticulin-3. These cleavages resulted in removal of either a dipeptide or a
    hexapeptide from the C-terminus of calreticulin-3 encompassing part or all of
    the ER retrieval signal. Consistently, expression of the calreticulin-3 derivative
    mimicking the phytaspase cleavage product in Nicotiana benthamiana cells demonstrated
    loss of the ER accumulation of the protein. Notably, upon its escape from the
    ER, calreticulin-3 was further processed by an unknown protease(s) to generate
    the free N-terminal (N) domain of calreticulin-3, which was ultimately secreted
    into the apoplast. Our study thus identified a specific proteolytic enzyme capable
    of precise detachment of the ER retrieval signal from a plant ER resident protein,
    with implications for the further fate of the escaped resident.
acknowledgement: "We thank C.U.T. Hellen for critically reading the manuscript. The
  MALDI MS facility and CLSM became available to us in the framework of Moscow State
  University Development Programs PNG 5.13 and PNR 5.13.\r\nThis work was funded by
  the Russian Science Foundation, grant numbers 19-14-00010 and 22-14-00071."
article_number: '16527'
article_processing_charge: Yes
article_type: original
author:
- first_name: Anastasiia
  full_name: Teplova, Anastasiia
  id: e3736151-106c-11ec-b916-c2558e2762c6
  last_name: Teplova
- first_name: Artemii A.
  full_name: Pigidanov, Artemii A.
  last_name: Pigidanov
- first_name: Marina V.
  full_name: Serebryakova, Marina V.
  last_name: Serebryakova
- first_name: Sergei A.
  full_name: Golyshev, Sergei A.
  last_name: Golyshev
- first_name: Raisa A.
  full_name: Galiullina, Raisa A.
  last_name: Galiullina
- first_name: Nina V.
  full_name: Chichkova, Nina V.
  last_name: Chichkova
- first_name: Andrey B.
  full_name: Vartapetian, Andrey B.
  last_name: Vartapetian
citation:
  ama: Teplova A, Pigidanov AA, Serebryakova MV, et al. Phytaspase Is capable of detaching
    the endoplasmic reticulum retrieval signal from tobacco calreticulin-3. <i>International
    Journal of Molecular Sciences</i>. 2023;24(22). doi:<a href="https://doi.org/10.3390/ijms242216527">10.3390/ijms242216527</a>
  apa: Teplova, A., Pigidanov, A. A., Serebryakova, M. V., Golyshev, S. A., Galiullina,
    R. A., Chichkova, N. V., &#38; Vartapetian, A. B. (2023). Phytaspase Is capable
    of detaching the endoplasmic reticulum retrieval signal from tobacco calreticulin-3.
    <i>International Journal of Molecular Sciences</i>. MDPI. <a href="https://doi.org/10.3390/ijms242216527">https://doi.org/10.3390/ijms242216527</a>
  chicago: Teplova, Anastasiia, Artemii A. Pigidanov, Marina V. Serebryakova, Sergei
    A. Golyshev, Raisa A. Galiullina, Nina V. Chichkova, and Andrey B. Vartapetian.
    “Phytaspase Is Capable of Detaching the Endoplasmic Reticulum Retrieval Signal
    from Tobacco Calreticulin-3.” <i>International Journal of Molecular Sciences</i>.
    MDPI, 2023. <a href="https://doi.org/10.3390/ijms242216527">https://doi.org/10.3390/ijms242216527</a>.
  ieee: A. Teplova <i>et al.</i>, “Phytaspase Is capable of detaching the endoplasmic
    reticulum retrieval signal from tobacco calreticulin-3,” <i>International Journal
    of Molecular Sciences</i>, vol. 24, no. 22. MDPI, 2023.
  ista: Teplova A, Pigidanov AA, Serebryakova MV, Golyshev SA, Galiullina RA, Chichkova
    NV, Vartapetian AB. 2023. Phytaspase Is capable of detaching the endoplasmic reticulum
    retrieval signal from tobacco calreticulin-3. International Journal of Molecular
    Sciences. 24(22), 16527.
  mla: Teplova, Anastasiia, et al. “Phytaspase Is Capable of Detaching the Endoplasmic
    Reticulum Retrieval Signal from Tobacco Calreticulin-3.” <i>International Journal
    of Molecular Sciences</i>, vol. 24, no. 22, 16527, MDPI, 2023, doi:<a href="https://doi.org/10.3390/ijms242216527">10.3390/ijms242216527</a>.
  short: A. Teplova, A.A. Pigidanov, M.V. Serebryakova, S.A. Golyshev, R.A. Galiullina,
    N.V. Chichkova, A.B. Vartapetian, International Journal of Molecular Sciences
    24 (2023).
date_created: 2024-01-10T09:24:35Z
date_published: 2023-11-01T00:00:00Z
date_updated: 2024-01-10T13:41:10Z
day: '01'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.3390/ijms242216527
external_id:
  isi:
  - '001113792600001'
  pmid:
  - '38003717'
file:
- access_level: open_access
  checksum: 4df7d206ba022b7f54eff1f0aec1659a
  content_type: application/pdf
  creator: dernst
  date_created: 2024-01-10T13:39:42Z
  date_updated: 2024-01-10T13:39:42Z
  file_id: '14791'
  file_name: 2023_IJMS_Teplova.pdf
  file_size: 2637784
  relation: main_file
  success: 1
file_date_updated: 2024-01-10T13:39:42Z
has_accepted_license: '1'
intvolume: '        24'
isi: 1
issue: '22'
keyword:
- Inorganic Chemistry
- Organic Chemistry
- Physical and Theoretical Chemistry
- Computer Science Applications
- Spectroscopy
- Molecular Biology
- General Medicine
- Catalysis
language:
- iso: eng
month: '11'
oa: 1
oa_version: Published Version
pmid: 1
publication: International Journal of Molecular Sciences
publication_identifier:
  issn:
  - 1422-0067
publication_status: published
publisher: MDPI
quality_controlled: '1'
status: public
title: Phytaspase Is capable of detaching the endoplasmic reticulum retrieval signal
  from tobacco calreticulin-3
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 24
year: '2023'
...
---
_id: '12921'
abstract:
- lang: eng
  text: Visible-light photocatalysis provides numerous useful methodologies for synthetic
    organic chemistry. However, the mechanisms of these reactions are often not fully
    understood. Common mechanistic experiments mainly aim to characterize excited
    state properties of photocatalysts and their interaction with other species. Recently,
    in situ reaction monitoring using dedicated techniques was shown to be well-suited
    for the identification of intermediates and to obtain kinetic insights, thereby
    providing more holistic pictures of the reactions of interest. This minireview
    surveys these technologies and discusses selected examples where reaction monitoring
    was used to elucidate the mechanism of photocatalytic reactions.
article_number: e202201583
article_processing_charge: No
article_type: original
author:
- first_name: Amiera
  full_name: Madani, Amiera
  last_name: Madani
- first_name: Bartholomäus
  full_name: Pieber, Bartholomäus
  id: 93e5e5b2-0da6-11ed-8a41-af589a024726
  last_name: Pieber
  orcid: 0000-0001-8689-388X
citation:
  ama: Madani A, Pieber B. In situ reaction monitoring in photocatalytic organic synthesis.
    <i>ChemCatChem</i>. 2023;15(7). doi:<a href="https://doi.org/10.1002/cctc.202201583">10.1002/cctc.202201583</a>
  apa: Madani, A., &#38; Pieber, B. (2023). In situ reaction monitoring in photocatalytic
    organic synthesis. <i>ChemCatChem</i>. Wiley. <a href="https://doi.org/10.1002/cctc.202201583">https://doi.org/10.1002/cctc.202201583</a>
  chicago: Madani, Amiera, and Bartholomäus Pieber. “In Situ Reaction Monitoring in
    Photocatalytic Organic Synthesis.” <i>ChemCatChem</i>. Wiley, 2023. <a href="https://doi.org/10.1002/cctc.202201583">https://doi.org/10.1002/cctc.202201583</a>.
  ieee: A. Madani and B. Pieber, “In situ reaction monitoring in photocatalytic organic
    synthesis,” <i>ChemCatChem</i>, vol. 15, no. 7. Wiley, 2023.
  ista: Madani A, Pieber B. 2023. In situ reaction monitoring in photocatalytic organic
    synthesis. ChemCatChem. 15(7), e202201583.
  mla: Madani, Amiera, and Bartholomäus Pieber. “In Situ Reaction Monitoring in Photocatalytic
    Organic Synthesis.” <i>ChemCatChem</i>, vol. 15, no. 7, e202201583, Wiley, 2023,
    doi:<a href="https://doi.org/10.1002/cctc.202201583">10.1002/cctc.202201583</a>.
  short: A. Madani, B. Pieber, ChemCatChem 15 (2023).
date_created: 2023-05-08T08:25:55Z
date_published: 2023-04-06T00:00:00Z
date_updated: 2023-05-15T08:35:48Z
day: '06'
doi: 10.1002/cctc.202201583
extern: '1'
intvolume: '        15'
issue: '7'
keyword:
- Inorganic Chemistry
- Organic Chemistry
- Physical and Theoretical Chemistry
- Catalysis
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: https://doi.org/10.1002/cctc.202201583
month: '04'
oa: 1
oa_version: Published Version
publication: ChemCatChem
publication_identifier:
  eissn:
  - 1867-3899
  issn:
  - 1867-3880
publication_status: published
publisher: Wiley
quality_controlled: '1'
scopus_import: '1'
status: public
title: In situ reaction monitoring in photocatalytic organic synthesis
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 15
year: '2023'
...
---
_id: '10392'
abstract:
- lang: eng
  text: Protonated formylmetallocenes [M(C5H5)(C5H4-CHOH)]+ (M = Fe, Ru) and their
    isomers have been studied at the BP86 and B3LYP levels of density functional theory.
    Oxygen-protonated isomers are the most stable forms in each case, with a plethora
    of ring- or metal-protonated species at least ca. 14 and 10 kcal/mol higher in
    energy for M = Fe and Ru, respectively. The computed rotational barriers around
    the C−C bond connecting the cyclopentadienyl and protonated formyl moieties, ca.
    18 kcal/mol, are indicative of substantial conjugation between these moieties.
    Some of the ring- and iron-protonated species are models for possible intermediates
    in Friedel–Crafts acylation of ferrocene, and the computations provide further
    evidence that exo attack is clearly favored over endo attack of the electrophile
    in this reaction. The structures of the most stable mono- and diprotonated formylferrocenes
    are corroborated by the good agreement between GIAO-B3LYP-computed and experimental
    NMR chemical shifts.
acknowledgement: M.B. wishes to thank Prof. W. Thiel and the Max-Planck-Institut für
  Kohlenforschung for continuing support. A Humboldt fellowship for V.V. is gratefully
  acknowledged. Computations were performed on Compaq XP1000 and ES40 workstations
  as well as on an Intel Xeon PC cluster at the MPI Mülheim. A.S. thanks the Computing
  Center of the University of Zagreb SRCE for allocating computer time on the Isabella
  cluster.
article_processing_charge: No
article_type: original
author:
- first_name: Anđela
  full_name: Šarić, Anđela
  id: bf63d406-f056-11eb-b41d-f263a6566d8b
  last_name: Šarić
  orcid: 0000-0002-7854-2139
- first_name: Valerije
  full_name: Vrček, Valerije
  last_name: Vrček
- first_name: Michael
  full_name: Bühl, Michael
  last_name: Bühl
citation:
  ama: Šarić A, Vrček V, Bühl M. Density functional study of protonated formylmetallocenes.
    <i>Organometallics</i>. 2008;27(3):394-401. doi:<a href="https://doi.org/10.1021/om700916f">10.1021/om700916f</a>
  apa: Šarić, A., Vrček, V., &#38; Bühl, M. (2008). Density functional study of protonated
    formylmetallocenes. <i>Organometallics</i>. American Chemical Society. <a href="https://doi.org/10.1021/om700916f">https://doi.org/10.1021/om700916f</a>
  chicago: Šarić, Anđela, Valerije Vrček, and Michael Bühl. “Density Functional Study
    of Protonated Formylmetallocenes.” <i>Organometallics</i>. American Chemical Society,
    2008. <a href="https://doi.org/10.1021/om700916f">https://doi.org/10.1021/om700916f</a>.
  ieee: A. Šarić, V. Vrček, and M. Bühl, “Density functional study of protonated formylmetallocenes,”
    <i>Organometallics</i>, vol. 27, no. 3. American Chemical Society, pp. 394–401,
    2008.
  ista: Šarić A, Vrček V, Bühl M. 2008. Density functional study of protonated formylmetallocenes.
    Organometallics. 27(3), 394–401.
  mla: Šarić, Anđela, et al. “Density Functional Study of Protonated Formylmetallocenes.”
    <i>Organometallics</i>, vol. 27, no. 3, American Chemical Society, 2008, pp. 394–401,
    doi:<a href="https://doi.org/10.1021/om700916f">10.1021/om700916f</a>.
  short: A. Šarić, V. Vrček, M. Bühl, Organometallics 27 (2008) 394–401.
date_created: 2021-11-29T15:31:06Z
date_published: 2008-01-15T00:00:00Z
date_updated: 2021-11-30T08:04:44Z
day: '15'
doi: 10.1021/om700916f
extern: '1'
intvolume: '        27'
issue: '3'
keyword:
- Inorganic Chemistry
- Organic Chemistry
- Physical and Theoretical Chemistry
language:
- iso: eng
main_file_link:
- url: https://pubs.acs.org/doi/10.1021/om700916f
month: '01'
oa_version: None
page: 394-401
publication: Organometallics
publication_identifier:
  eissn:
  - 1520-6041
  issn:
  - 0276-7333
publication_status: published
publisher: American Chemical Society
quality_controlled: '1'
scopus_import: '1'
status: public
title: Density functional study of protonated formylmetallocenes
type: journal_article
user_id: 8b945eb4-e2f2-11eb-945a-df72226e66a9
volume: 27
year: '2008'
...