---
_id: '14834'
abstract:
- lang: eng
  text: Bacteria divide by binary fission. The protein machine responsible for this
    process is the divisome, a transient assembly of more than 30 proteins in and
    on the surface of the cytoplasmic membrane. Together, they constrict the cell
    envelope and remodel the peptidoglycan layer to eventually split the cell into
    two. For Escherichia coli, most molecular players involved in this process have
    probably been identified, but obtaining the quantitative information needed for
    a mechanistic understanding can often not be achieved from experiments in vivo
    alone. Since the discovery of the Z-ring more than 30 years ago, in vitro reconstitution
    experiments have been crucial to shed light on molecular processes normally hidden
    in the complex environment of the living cell. In this review, we summarize how
    rebuilding the divisome from purified components – or at least parts of it - have
    been instrumental to obtain the detailed mechanistic understanding of the bacterial
    cell division machinery that we have today.
acknowledgement: We acknowledge members of the Loose laboratory at ISTA for helpful
  discussions—in particular M. Kojic for his insightful comments. This work was supported
  by the Austrian Science Fund (FWF P34607) to M.L.
article_number: '151380'
article_processing_charge: Yes
article_type: review
author:
- first_name: Philipp
  full_name: Radler, Philipp
  id: 40136C2A-F248-11E8-B48F-1D18A9856A87
  last_name: Radler
  orcid: '0000-0001-9198-2182 '
- first_name: Martin
  full_name: Loose, Martin
  id: 462D4284-F248-11E8-B48F-1D18A9856A87
  last_name: Loose
  orcid: 0000-0001-7309-9724
citation:
  ama: 'Radler P, Loose M. A dynamic duo: Understanding the roles of FtsZ and FtsA
    for Escherichia coli cell division through in vitro approaches. <i>European Journal
    of Cell Biology</i>. 2024;103(1). doi:<a href="https://doi.org/10.1016/j.ejcb.2023.151380">10.1016/j.ejcb.2023.151380</a>'
  apa: 'Radler, P., &#38; Loose, M. (2024). A dynamic duo: Understanding the roles
    of FtsZ and FtsA for Escherichia coli cell division through in vitro approaches.
    <i>European Journal of Cell Biology</i>. Elsevier. <a href="https://doi.org/10.1016/j.ejcb.2023.151380">https://doi.org/10.1016/j.ejcb.2023.151380</a>'
  chicago: 'Radler, Philipp, and Martin Loose. “A Dynamic Duo: Understanding the Roles
    of FtsZ and FtsA for Escherichia Coli Cell Division through in Vitro Approaches.”
    <i>European Journal of Cell Biology</i>. Elsevier, 2024. <a href="https://doi.org/10.1016/j.ejcb.2023.151380">https://doi.org/10.1016/j.ejcb.2023.151380</a>.'
  ieee: 'P. Radler and M. Loose, “A dynamic duo: Understanding the roles of FtsZ and
    FtsA for Escherichia coli cell division through in vitro approaches,” <i>European
    Journal of Cell Biology</i>, vol. 103, no. 1. Elsevier, 2024.'
  ista: 'Radler P, Loose M. 2024. A dynamic duo: Understanding the roles of FtsZ and
    FtsA for Escherichia coli cell division through in vitro approaches. European
    Journal of Cell Biology. 103(1), 151380.'
  mla: 'Radler, Philipp, and Martin Loose. “A Dynamic Duo: Understanding the Roles
    of FtsZ and FtsA for Escherichia Coli Cell Division through in Vitro Approaches.”
    <i>European Journal of Cell Biology</i>, vol. 103, no. 1, 151380, Elsevier, 2024,
    doi:<a href="https://doi.org/10.1016/j.ejcb.2023.151380">10.1016/j.ejcb.2023.151380</a>.'
  short: P. Radler, M. Loose, European Journal of Cell Biology 103 (2024).
date_created: 2024-01-18T08:16:43Z
date_published: 2024-01-12T00:00:00Z
date_updated: 2024-01-23T08:37:13Z
day: '12'
ddc:
- '570'
department:
- _id: MaLo
doi: 10.1016/j.ejcb.2023.151380
external_id:
  pmid:
  - '38218128'
has_accepted_license: '1'
intvolume: '       103'
issue: '1'
keyword:
- Cell Biology
- General Medicine
- Histology
- Pathology and Forensic Medicine
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: https://doi.org/10.1016/j.ejcb.2023.151380
month: '01'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: fc38323b-9c52-11eb-aca3-ff8afb4a011d
  grant_number: P34607
  name: "Understanding bacterial cell division by in vitro\r\nreconstitution"
publication: European Journal of Cell Biology
publication_identifier:
  issn:
  - 0171-9335
publication_status: epub_ahead
publisher: Elsevier
quality_controlled: '1'
scopus_import: '1'
status: public
title: 'A dynamic duo: Understanding the roles of FtsZ and FtsA for Escherichia coli
  cell division through in vitro approaches'
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 103
year: '2024'
...
---
_id: '11078'
abstract:
- lang: eng
  text: Aging is associated with the decline of protein, cell, and organ function.
    Here, we use an integrated approach to characterize gene expression, bulk translation,
    and cell biology in the brains and livers of young and old rats. We identify 468
    differences in protein abundance between young and old animals. The majority are
    a consequence of altered translation output, that is, the combined effect of changes
    in transcript abundance and translation efficiency. In addition, we identify 130
    proteins whose overall abundance remains unchanged but whose sub-cellular localization,
    phosphorylation state, or splice-form varies. While some protein-level differences
    appear to be a generic property of the rats’ chronological age, the majority are
    specific to one organ. These may be a consequence of the organ’s physiology or
    the chronological age of the cells within the tissue. Taken together, our study
    provides an initial view of the proteome at the molecular, sub-cellular, and organ
    level in young and old rats.
article_processing_charge: No
article_type: original
author:
- first_name: Alessandro
  full_name: Ori, Alessandro
  last_name: Ori
- first_name: Brandon H.
  full_name: Toyama, Brandon H.
  last_name: Toyama
- first_name: Michael S.
  full_name: Harris, Michael S.
  last_name: Harris
- first_name: Thomas
  full_name: Bock, Thomas
  last_name: Bock
- first_name: Murat
  full_name: Iskar, Murat
  last_name: Iskar
- first_name: Peer
  full_name: Bork, Peer
  last_name: Bork
- first_name: Nicholas T.
  full_name: Ingolia, Nicholas T.
  last_name: Ingolia
- first_name: Martin W
  full_name: HETZER, Martin W
  id: 86c0d31b-b4eb-11ec-ac5a-eae7b2e135ed
  last_name: HETZER
  orcid: 0000-0002-2111-992X
- first_name: Martin
  full_name: Beck, Martin
  last_name: Beck
citation:
  ama: Ori A, Toyama BH, Harris MS, et al. Integrated transcriptome and proteome analyses
    reveal organ-specific proteome deterioration in old rats. <i>Cell Systems</i>.
    2015;1(3):P224-237. doi:<a href="https://doi.org/10.1016/j.cels.2015.08.012">10.1016/j.cels.2015.08.012</a>
  apa: Ori, A., Toyama, B. H., Harris, M. S., Bock, T., Iskar, M., Bork, P., … Beck,
    M. (2015). Integrated transcriptome and proteome analyses reveal organ-specific
    proteome deterioration in old rats. <i>Cell Systems</i>. Elsevier. <a href="https://doi.org/10.1016/j.cels.2015.08.012">https://doi.org/10.1016/j.cels.2015.08.012</a>
  chicago: Ori, Alessandro, Brandon H. Toyama, Michael S. Harris, Thomas Bock, Murat
    Iskar, Peer Bork, Nicholas T. Ingolia, Martin Hetzer, and Martin Beck. “Integrated
    Transcriptome and Proteome Analyses Reveal Organ-Specific Proteome Deterioration
    in Old Rats.” <i>Cell Systems</i>. Elsevier, 2015. <a href="https://doi.org/10.1016/j.cels.2015.08.012">https://doi.org/10.1016/j.cels.2015.08.012</a>.
  ieee: A. Ori <i>et al.</i>, “Integrated transcriptome and proteome analyses reveal
    organ-specific proteome deterioration in old rats,” <i>Cell Systems</i>, vol.
    1, no. 3. Elsevier, pp. P224-237, 2015.
  ista: Ori A, Toyama BH, Harris MS, Bock T, Iskar M, Bork P, Ingolia NT, Hetzer M,
    Beck M. 2015. Integrated transcriptome and proteome analyses reveal organ-specific
    proteome deterioration in old rats. Cell Systems. 1(3), P224-237.
  mla: Ori, Alessandro, et al. “Integrated Transcriptome and Proteome Analyses Reveal
    Organ-Specific Proteome Deterioration in Old Rats.” <i>Cell Systems</i>, vol.
    1, no. 3, Elsevier, 2015, pp. P224-237, doi:<a href="https://doi.org/10.1016/j.cels.2015.08.012">10.1016/j.cels.2015.08.012</a>.
  short: A. Ori, B.H. Toyama, M.S. Harris, T. Bock, M. Iskar, P. Bork, N.T. Ingolia,
    M. Hetzer, M. Beck, Cell Systems 1 (2015) P224-237.
date_created: 2022-04-07T07:49:39Z
date_published: 2015-09-23T00:00:00Z
date_updated: 2022-07-18T08:44:07Z
day: '23'
doi: 10.1016/j.cels.2015.08.012
extern: '1'
external_id:
  pmid:
  - '27135913'
intvolume: '         1'
issue: '3'
keyword:
- Cell Biology
- Histology
- Pathology and Forensic Medicine
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: https://doi.org/10.1016/j.cels.2015.08.012
month: '09'
oa: 1
oa_version: Published Version
page: P224-237
pmid: 1
publication: Cell Systems
publication_identifier:
  issn:
  - 2405-4712
publication_status: published
publisher: Elsevier
quality_controlled: '1'
scopus_import: '1'
status: public
title: Integrated transcriptome and proteome analyses reveal organ-specific proteome
  deterioration in old rats
type: journal_article
user_id: 72615eeb-f1f3-11ec-aa25-d4573ddc34fd
volume: 1
year: '2015'
...