[{"pmid":1,"date_published":"2024-01-12T00:00:00Z","acknowledgement":"We acknowledge members of the Loose laboratory at ISTA for helpful discussions—in particular M. Kojic for his insightful comments. This work was supported by the Austrian Science Fund (FWF P34607) to M.L.","status":"public","publication":"European Journal of Cell Biology","project":[{"_id":"fc38323b-9c52-11eb-aca3-ff8afb4a011d","grant_number":"P34607","name":"Understanding bacterial cell division by in vitro\r\nreconstitution"}],"keyword":["Cell Biology","General Medicine","Histology","Pathology and Forensic Medicine"],"year":"2024","external_id":{"pmid":["38218128"]},"main_file_link":[{"open_access":"1","url":"https://doi.org/10.1016/j.ejcb.2023.151380"}],"quality_controlled":"1","ddc":["570"],"_id":"14834","date_updated":"2024-01-23T08:37:13Z","type":"journal_article","article_processing_charge":"Yes","doi":"10.1016/j.ejcb.2023.151380","publisher":"Elsevier","citation":{"apa":"Radler, P., &#38; Loose, M. (2024). A dynamic duo: Understanding the roles of FtsZ and FtsA for Escherichia coli cell division through in vitro approaches. <i>European Journal of Cell Biology</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.ejcb.2023.151380\">https://doi.org/10.1016/j.ejcb.2023.151380</a>","mla":"Radler, Philipp, and Martin Loose. “A Dynamic Duo: Understanding the Roles of FtsZ and FtsA for Escherichia Coli Cell Division through in Vitro Approaches.” <i>European Journal of Cell Biology</i>, vol. 103, no. 1, 151380, Elsevier, 2024, doi:<a href=\"https://doi.org/10.1016/j.ejcb.2023.151380\">10.1016/j.ejcb.2023.151380</a>.","chicago":"Radler, Philipp, and Martin Loose. “A Dynamic Duo: Understanding the Roles of FtsZ and FtsA for Escherichia Coli Cell Division through in Vitro Approaches.” <i>European Journal of Cell Biology</i>. Elsevier, 2024. <a href=\"https://doi.org/10.1016/j.ejcb.2023.151380\">https://doi.org/10.1016/j.ejcb.2023.151380</a>.","ista":"Radler P, Loose M. 2024. A dynamic duo: Understanding the roles of FtsZ and FtsA for Escherichia coli cell division through in vitro approaches. European Journal of Cell Biology. 103(1), 151380.","ieee":"P. Radler and M. Loose, “A dynamic duo: Understanding the roles of FtsZ and FtsA for Escherichia coli cell division through in vitro approaches,” <i>European Journal of Cell Biology</i>, vol. 103, no. 1. Elsevier, 2024.","short":"P. Radler, M. Loose, European Journal of Cell Biology 103 (2024).","ama":"Radler P, Loose M. A dynamic duo: Understanding the roles of FtsZ and FtsA for Escherichia coli cell division through in vitro approaches. <i>European Journal of Cell Biology</i>. 2024;103(1). doi:<a href=\"https://doi.org/10.1016/j.ejcb.2023.151380\">10.1016/j.ejcb.2023.151380</a>"},"issue":"1","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","oa":1,"language":[{"iso":"eng"}],"department":[{"_id":"MaLo"}],"article_number":"151380","month":"01","publication_identifier":{"issn":["0171-9335"]},"publication_status":"epub_ahead","has_accepted_license":"1","tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","short":"CC BY (4.0)"},"abstract":[{"text":"Bacteria divide by binary fission. The protein machine responsible for this process is the divisome, a transient assembly of more than 30 proteins in and on the surface of the cytoplasmic membrane. Together, they constrict the cell envelope and remodel the peptidoglycan layer to eventually split the cell into two. For Escherichia coli, most molecular players involved in this process have probably been identified, but obtaining the quantitative information needed for a mechanistic understanding can often not be achieved from experiments in vivo alone. Since the discovery of the Z-ring more than 30 years ago, in vitro reconstitution experiments have been crucial to shed light on molecular processes normally hidden in the complex environment of the living cell. In this review, we summarize how rebuilding the divisome from purified components – or at least parts of it - have been instrumental to obtain the detailed mechanistic understanding of the bacterial cell division machinery that we have today.","lang":"eng"}],"intvolume":"       103","volume":103,"date_created":"2024-01-18T08:16:43Z","article_type":"review","scopus_import":"1","day":"12","author":[{"full_name":"Radler, Philipp","id":"40136C2A-F248-11E8-B48F-1D18A9856A87","last_name":"Radler","first_name":"Philipp","orcid":"0000-0001-9198-2182 "},{"orcid":"0000-0001-7309-9724","first_name":"Martin","full_name":"Loose, Martin","id":"462D4284-F248-11E8-B48F-1D18A9856A87","last_name":"Loose"}],"oa_version":"Published Version","title":"A dynamic duo: Understanding the roles of FtsZ and FtsA for Escherichia coli cell division through in vitro approaches"},{"abstract":[{"text":"Aging is associated with the decline of protein, cell, and organ function. Here, we use an integrated approach to characterize gene expression, bulk translation, and cell biology in the brains and livers of young and old rats. We identify 468 differences in protein abundance between young and old animals. The majority are a consequence of altered translation output, that is, the combined effect of changes in transcript abundance and translation efficiency. In addition, we identify 130 proteins whose overall abundance remains unchanged but whose sub-cellular localization, phosphorylation state, or splice-form varies. While some protein-level differences appear to be a generic property of the rats’ chronological age, the majority are specific to one organ. These may be a consequence of the organ’s physiology or the chronological age of the cells within the tissue. Taken together, our study provides an initial view of the proteome at the molecular, sub-cellular, and organ level in young and old rats.","lang":"eng"}],"intvolume":"         1","publication_status":"published","publication_identifier":{"issn":["2405-4712"]},"oa_version":"Published Version","title":"Integrated transcriptome and proteome analyses reveal organ-specific proteome deterioration in old rats","author":[{"last_name":"Ori","full_name":"Ori, Alessandro","first_name":"Alessandro"},{"first_name":"Brandon H.","full_name":"Toyama, Brandon H.","last_name":"Toyama"},{"first_name":"Michael S.","last_name":"Harris","full_name":"Harris, Michael S."},{"last_name":"Bock","full_name":"Bock, Thomas","first_name":"Thomas"},{"last_name":"Iskar","full_name":"Iskar, Murat","first_name":"Murat"},{"first_name":"Peer","last_name":"Bork","full_name":"Bork, Peer"},{"first_name":"Nicholas T.","full_name":"Ingolia, Nicholas T.","last_name":"Ingolia"},{"id":"86c0d31b-b4eb-11ec-ac5a-eae7b2e135ed","full_name":"HETZER, Martin W","last_name":"HETZER","orcid":"0000-0002-2111-992X","first_name":"Martin W"},{"first_name":"Martin","last_name":"Beck","full_name":"Beck, Martin"}],"scopus_import":"1","day":"23","article_type":"original","date_created":"2022-04-07T07:49:39Z","volume":1,"language":[{"iso":"eng"}],"oa":1,"user_id":"72615eeb-f1f3-11ec-aa25-d4573ddc34fd","issue":"3","citation":{"ama":"Ori A, Toyama BH, Harris MS, et al. Integrated transcriptome and proteome analyses reveal organ-specific proteome deterioration in old rats. <i>Cell Systems</i>. 2015;1(3):P224-237. doi:<a href=\"https://doi.org/10.1016/j.cels.2015.08.012\">10.1016/j.cels.2015.08.012</a>","short":"A. Ori, B.H. Toyama, M.S. Harris, T. Bock, M. Iskar, P. Bork, N.T. Ingolia, M. Hetzer, M. Beck, Cell Systems 1 (2015) P224-237.","ieee":"A. Ori <i>et al.</i>, “Integrated transcriptome and proteome analyses reveal organ-specific proteome deterioration in old rats,” <i>Cell Systems</i>, vol. 1, no. 3. Elsevier, pp. P224-237, 2015.","ista":"Ori A, Toyama BH, Harris MS, Bock T, Iskar M, Bork P, Ingolia NT, Hetzer M, Beck M. 2015. Integrated transcriptome and proteome analyses reveal organ-specific proteome deterioration in old rats. Cell Systems. 1(3), P224-237.","chicago":"Ori, Alessandro, Brandon H. Toyama, Michael S. Harris, Thomas Bock, Murat Iskar, Peer Bork, Nicholas T. Ingolia, Martin Hetzer, and Martin Beck. “Integrated Transcriptome and Proteome Analyses Reveal Organ-Specific Proteome Deterioration in Old Rats.” <i>Cell Systems</i>. Elsevier, 2015. <a href=\"https://doi.org/10.1016/j.cels.2015.08.012\">https://doi.org/10.1016/j.cels.2015.08.012</a>.","mla":"Ori, Alessandro, et al. “Integrated Transcriptome and Proteome Analyses Reveal Organ-Specific Proteome Deterioration in Old Rats.” <i>Cell Systems</i>, vol. 1, no. 3, Elsevier, 2015, pp. P224-237, doi:<a href=\"https://doi.org/10.1016/j.cels.2015.08.012\">10.1016/j.cels.2015.08.012</a>.","apa":"Ori, A., Toyama, B. H., Harris, M. S., Bock, T., Iskar, M., Bork, P., … Beck, M. (2015). Integrated transcriptome and proteome analyses reveal organ-specific proteome deterioration in old rats. <i>Cell Systems</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.cels.2015.08.012\">https://doi.org/10.1016/j.cels.2015.08.012</a>"},"month":"09","page":"P224-237","quality_controlled":"1","main_file_link":[{"url":"https://doi.org/10.1016/j.cels.2015.08.012","open_access":"1"}],"publisher":"Elsevier","doi":"10.1016/j.cels.2015.08.012","article_processing_charge":"No","type":"journal_article","date_updated":"2022-07-18T08:44:07Z","_id":"11078","extern":"1","status":"public","publication":"Cell Systems","date_published":"2015-09-23T00:00:00Z","pmid":1,"external_id":{"pmid":["27135913"]},"year":"2015","keyword":["Cell Biology","Histology","Pathology and Forensic Medicine"]}]