---
_id: '9245'
abstract:
- lang: eng
  text: Tissue morphogenesis is driven by mechanical forces triggering cell movements
    and shape changes. Quantitatively measuring tension within tissues is of great
    importance for understanding the role of mechanical signals acting on the cell
    and tissue level during morphogenesis. Here we introduce laser ablation as a useful
    tool to probe tissue tension within the granulosa layer, an epithelial monolayer
    of somatic cells that surround the zebrafish female gamete during folliculogenesis.
    We describe in detail how to isolate follicles, mount samples, perform laser surgery,
    and analyze the data.
acknowledged_ssus:
- _id: Bio
- _id: PreCl
acknowledgement: We thank Prof. Masazumi Tada and Roland Dosch for providing transgenic
  zebrafish lines, the Heisenberg lab for technical assistance and feedback on the
  manuscript, and the Bioimaging and Fish facilities of IST Austria for continuous
  support. This work was funded by an ERC advanced grant (MECSPEC to C.-P.H.).
alternative_title:
- Methods in Molecular Biology
article_processing_charge: No
author:
- first_name: Peng
  full_name: Xia, Peng
  id: 4AB6C7D0-F248-11E8-B48F-1D18A9856A87
  last_name: Xia
  orcid: 0000-0002-5419-7756
- first_name: Carl-Philipp J
  full_name: Heisenberg, Carl-Philipp J
  id: 39427864-F248-11E8-B48F-1D18A9856A87
  last_name: Heisenberg
  orcid: 0000-0002-0912-4566
citation:
  ama: 'Xia P, Heisenberg C-PJ. Quantifying tissue tension in the granulosa layer
    after laser surgery. In: Dosch R, ed. <i>Germline Development in the Zebrafish</i>.
    Vol 2218. Humana; 2021:117-128. doi:<a href="https://doi.org/10.1007/978-1-0716-0970-5_10">10.1007/978-1-0716-0970-5_10</a>'
  apa: Xia, P., &#38; Heisenberg, C.-P. J. (2021). Quantifying tissue tension in the
    granulosa layer after laser surgery. In R. Dosch (Ed.), <i>Germline Development
    in the Zebrafish</i> (Vol. 2218, pp. 117–128). Humana. <a href="https://doi.org/10.1007/978-1-0716-0970-5_10">https://doi.org/10.1007/978-1-0716-0970-5_10</a>
  chicago: Xia, Peng, and Carl-Philipp J Heisenberg. “Quantifying Tissue Tension in
    the Granulosa Layer after Laser Surgery.” In <i>Germline Development in the Zebrafish</i>,
    edited by Roland Dosch, 2218:117–28. Humana, 2021. <a href="https://doi.org/10.1007/978-1-0716-0970-5_10">https://doi.org/10.1007/978-1-0716-0970-5_10</a>.
  ieee: P. Xia and C.-P. J. Heisenberg, “Quantifying tissue tension in the granulosa
    layer after laser surgery,” in <i>Germline Development in the Zebrafish</i>, vol.
    2218, R. Dosch, Ed. Humana, 2021, pp. 117–128.
  ista: 'Xia P, Heisenberg C-PJ. 2021.Quantifying tissue tension in the granulosa
    layer after laser surgery. In: Germline Development in the Zebrafish. Methods
    in Molecular Biology, vol. 2218, 117–128.'
  mla: Xia, Peng, and Carl-Philipp J. Heisenberg. “Quantifying Tissue Tension in the
    Granulosa Layer after Laser Surgery.” <i>Germline Development in the Zebrafish</i>,
    edited by Roland Dosch, vol. 2218, Humana, 2021, pp. 117–28, doi:<a href="https://doi.org/10.1007/978-1-0716-0970-5_10">10.1007/978-1-0716-0970-5_10</a>.
  short: P. Xia, C.-P.J. Heisenberg, in:, R. Dosch (Ed.), Germline Development in
    the Zebrafish, Humana, 2021, pp. 117–128.
date_created: 2021-03-14T23:01:34Z
date_published: 2021-02-20T00:00:00Z
date_updated: 2022-06-03T10:57:55Z
day: '20'
department:
- _id: CaHe
doi: 10.1007/978-1-0716-0970-5_10
ec_funded: 1
editor:
- first_name: Roland
  full_name: Dosch, Roland
  last_name: Dosch
external_id:
  pmid:
  - '33606227'
intvolume: '      2218'
keyword:
- Tissue tension
- Morphogenesis
- Laser ablation
- Zebrafish folliculogenesis
- Granulosa cells
language:
- iso: eng
month: '02'
oa_version: None
page: 117-128
pmid: 1
project:
- _id: 260F1432-B435-11E9-9278-68D0E5697425
  call_identifier: H2020
  grant_number: '742573'
  name: Interaction and feedback between cell mechanics and fate specification in
    vertebrate gastrulation
publication: Germline Development in the Zebrafish
publication_identifier:
  eisbn:
  - 978-1-0716-0970-5
  eissn:
  - 1940-6029
  isbn:
  - 978-1-0716-0969-9
  issn:
  - 1064-3745
publication_status: published
publisher: Humana
quality_controlled: '1'
scopus_import: '1'
status: public
title: Quantifying tissue tension in the granulosa layer after laser surgery
type: book_chapter
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 2218
year: '2021'
...