---
_id: '12173'
abstract:
- lang: eng
  text: With increasing urbanization and industrialization, the prevalence of inflammatory
    bowel diseases (IBDs) has steadily been rising over the past two decades. IBD
    involves flares of gastrointestinal (GI) inflammation accompanied by microbiota
    perturbations. However, microbial mechanisms that trigger such flares remain elusive.
    Here, we analyzed the association of the emerging pathogen atypical enteropathogenic
    E. coli (aEPEC) with IBD disease activity. The presence of diarrheagenic E. coli
    was assessed in stool samples from 630 IBD patients and 234 age- and sex-matched
    controls without GI symptoms. Microbiota was analyzed with 16S ribosomal RNA gene
    amplicon sequencing, and 57 clinical aEPEC isolates were subjected to whole-genome
    sequencing and in vitro pathogenicity experiments including biofilm formation,
    epithelial barrier function and the ability to induce pro-inflammatory signaling.
    The presence of aEPEC correlated with laboratory, clinical and endoscopic disease
    activity in ulcerative colitis (UC), as well as microbiota dysbiosis. In vitro,
    aEPEC strains induce epithelial p21-activated kinases, disrupt the epithelial
    barrier and display potent biofilm formation. The effector proteins espV and espG2
    distinguish aEPEC cultured from UC and Crohn’s disease patients, respectively.
    EspV-positive aEPEC harbor more virulence factors and have a higher pro-inflammatory
    potential, which is counteracted by 5-ASA. aEPEC may tip a fragile immune–microbiota
    homeostasis and thereby contribute to flares in UC. aEPEC isolates from UC patients
    display properties to disrupt the epithelial barrier and to induce pro-inflammatory
    signaling in vitro.
acknowledgement: "We would like to acknowledge Anita Krnjic, Christina Gmainer, Marion
  Nehr, Helga Mock, and Sena Ecin for technical support in conducting the experiments.\r\nThis
  study was supported by the Austrian Science Fund (P 32302) and the Vienna Science
  and Technology Fund (LS18- 053; Austrian Science Fund (FWF)) [P 32302]."
article_number: e2143218
article_processing_charge: No
article_type: original
author:
- first_name: Maximilian
  full_name: Baumgartner, Maximilian
  last_name: Baumgartner
- first_name: Rebecca
  full_name: Zirnbauer, Rebecca
  last_name: Zirnbauer
- first_name: Sabine
  full_name: Schlager, Sabine
  last_name: Schlager
- first_name: Daniel
  full_name: Mertens, Daniel
  last_name: Mertens
- first_name: Nikolaus
  full_name: Gasche, Nikolaus
  last_name: Gasche
- first_name: Barbara
  full_name: Sladek, Barbara
  last_name: Sladek
- first_name: Craig
  full_name: Herbold, Craig
  last_name: Herbold
- first_name: Olga
  full_name: Bochkareva, Olga
  last_name: Bochkareva
- first_name: Vera
  full_name: Emelianenko, Vera
  id: 20152b9d-927a-11ed-8107-be36d740812d
  last_name: Emelianenko
- first_name: Harald
  full_name: Vogelsang, Harald
  last_name: Vogelsang
- first_name: Michaela
  full_name: Lang, Michaela
  last_name: Lang
- first_name: Anton
  full_name: Klotz, Anton
  last_name: Klotz
- first_name: Birgit
  full_name: Moik, Birgit
  last_name: Moik
- first_name: Athanasios
  full_name: Makristathis, Athanasios
  last_name: Makristathis
- first_name: David
  full_name: Berry, David
  last_name: Berry
- first_name: Stefanie
  full_name: Dabsch, Stefanie
  last_name: Dabsch
- first_name: Vineeta
  full_name: Khare, Vineeta
  last_name: Khare
- first_name: Christoph
  full_name: Gasche, Christoph
  last_name: Gasche
citation:
  ama: Baumgartner M, Zirnbauer R, Schlager S, et al. Atypical enteropathogenic E.
    coli are associated with disease activity in ulcerative colitis. <i>Gut Microbes</i>.
    2022;14(1). doi:<a href="https://doi.org/10.1080/19490976.2022.2143218">10.1080/19490976.2022.2143218</a>
  apa: Baumgartner, M., Zirnbauer, R., Schlager, S., Mertens, D., Gasche, N., Sladek,
    B., … Gasche, C. (2022). Atypical enteropathogenic E. coli are associated with
    disease activity in ulcerative colitis. <i>Gut Microbes</i>. Taylor &#38; Francis.
    <a href="https://doi.org/10.1080/19490976.2022.2143218">https://doi.org/10.1080/19490976.2022.2143218</a>
  chicago: Baumgartner, Maximilian, Rebecca Zirnbauer, Sabine Schlager, Daniel Mertens,
    Nikolaus Gasche, Barbara Sladek, Craig Herbold, et al. “Atypical Enteropathogenic
    E. Coli Are Associated with Disease Activity in Ulcerative Colitis.” <i>Gut Microbes</i>.
    Taylor &#38; Francis, 2022. <a href="https://doi.org/10.1080/19490976.2022.2143218">https://doi.org/10.1080/19490976.2022.2143218</a>.
  ieee: M. Baumgartner <i>et al.</i>, “Atypical enteropathogenic E. coli are associated
    with disease activity in ulcerative colitis,” <i>Gut Microbes</i>, vol. 14, no.
    1. Taylor &#38; Francis, 2022.
  ista: Baumgartner M, Zirnbauer R, Schlager S, Mertens D, Gasche N, Sladek B, Herbold
    C, Bochkareva O, Emelianenko V, Vogelsang H, Lang M, Klotz A, Moik B, Makristathis
    A, Berry D, Dabsch S, Khare V, Gasche C. 2022. Atypical enteropathogenic E. coli
    are associated with disease activity in ulcerative colitis. Gut Microbes. 14(1),
    e2143218.
  mla: Baumgartner, Maximilian, et al. “Atypical Enteropathogenic E. Coli Are Associated
    with Disease Activity in Ulcerative Colitis.” <i>Gut Microbes</i>, vol. 14, no.
    1, e2143218, Taylor &#38; Francis, 2022, doi:<a href="https://doi.org/10.1080/19490976.2022.2143218">10.1080/19490976.2022.2143218</a>.
  short: M. Baumgartner, R. Zirnbauer, S. Schlager, D. Mertens, N. Gasche, B. Sladek,
    C. Herbold, O. Bochkareva, V. Emelianenko, H. Vogelsang, M. Lang, A. Klotz, B.
    Moik, A. Makristathis, D. Berry, S. Dabsch, V. Khare, C. Gasche, Gut Microbes
    14 (2022).
date_created: 2023-01-12T12:11:36Z
date_published: 2022-11-22T00:00:00Z
date_updated: 2023-08-04T09:10:18Z
day: '22'
ddc:
- '570'
department:
- _id: FyKo
doi: 10.1080/19490976.2022.2143218
external_id:
  isi:
  - '000889180100001'
file:
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  creator: dernst
  date_created: 2023-01-26T10:56:51Z
  date_updated: 2023-01-26T10:56:51Z
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file_date_updated: 2023-01-26T10:56:51Z
has_accepted_license: '1'
intvolume: '        14'
isi: 1
issue: '1'
keyword:
- Infectious Diseases
- Microbiology (medical)
- Gastroenterology
- Microbiology
language:
- iso: eng
month: '11'
oa: 1
oa_version: Published Version
publication: Gut Microbes
publication_identifier:
  eissn:
  - 1949-0984
  issn:
  - 1949-0976
publication_status: published
publisher: Taylor & Francis
quality_controlled: '1'
scopus_import: '1'
status: public
title: Atypical enteropathogenic E. coli are associated with disease activity in ulcerative
  colitis
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 14
year: '2022'
...