---
_id: '6354'
abstract:
- lang: eng
  text: Blood platelets are critical for hemostasis and thrombosis, but also play
    diverse roles during immune responses. We have recently reported that platelets
    migrate at sites of infection in vitro and in vivo. Importantly, platelets use
    their ability to migrate to collect and bundle fibrin (ogen)-bound bacteria accomplishing
    efficient intravascular bacterial trapping. Here, we describe a method that allows
    analyzing platelet migration in vitro, focusing on their ability to collect bacteria
    and trap bacteria under flow.
acknowledgement: ' FöFoLe project 947 (F.G.), the Friedrich-Baur-Stiftung project
  41/16 (F.G.)'
article_number: e3018
author:
- first_name: Shuxia
  full_name: Fan, Shuxia
  last_name: Fan
- first_name: Michael
  full_name: Lorenz, Michael
  last_name: Lorenz
- first_name: Steffen
  full_name: Massberg, Steffen
  last_name: Massberg
- first_name: Florian R
  full_name: Gärtner, Florian R
  id: 397A88EE-F248-11E8-B48F-1D18A9856A87
  last_name: Gärtner
  orcid: 0000-0001-6120-3723
citation:
  ama: Fan S, Lorenz M, Massberg S, Gärtner FR. Platelet migration and bacterial trapping
    assay under flow. <i>Bio-Protocol</i>. 2018;8(18). doi:<a href="https://doi.org/10.21769/bioprotoc.3018">10.21769/bioprotoc.3018</a>
  apa: Fan, S., Lorenz, M., Massberg, S., &#38; Gärtner, F. R. (2018). Platelet migration
    and bacterial trapping assay under flow. <i>Bio-Protocol</i>. Bio-Protocol. <a
    href="https://doi.org/10.21769/bioprotoc.3018">https://doi.org/10.21769/bioprotoc.3018</a>
  chicago: Fan, Shuxia, Michael Lorenz, Steffen Massberg, and Florian R Gärtner. “Platelet
    Migration and Bacterial Trapping Assay under Flow.” <i>Bio-Protocol</i>. Bio-Protocol,
    2018. <a href="https://doi.org/10.21769/bioprotoc.3018">https://doi.org/10.21769/bioprotoc.3018</a>.
  ieee: S. Fan, M. Lorenz, S. Massberg, and F. R. Gärtner, “Platelet migration and
    bacterial trapping assay under flow,” <i>Bio-Protocol</i>, vol. 8, no. 18. Bio-Protocol,
    2018.
  ista: Fan S, Lorenz M, Massberg S, Gärtner FR. 2018. Platelet migration and bacterial
    trapping assay under flow. Bio-Protocol. 8(18), e3018.
  mla: Fan, Shuxia, et al. “Platelet Migration and Bacterial Trapping Assay under
    Flow.” <i>Bio-Protocol</i>, vol. 8, no. 18, e3018, Bio-Protocol, 2018, doi:<a
    href="https://doi.org/10.21769/bioprotoc.3018">10.21769/bioprotoc.3018</a>.
  short: S. Fan, M. Lorenz, S. Massberg, F.R. Gärtner, Bio-Protocol 8 (2018).
date_created: 2019-04-29T09:40:33Z
date_published: 2018-09-20T00:00:00Z
date_updated: 2021-01-12T08:07:12Z
day: '20'
ddc:
- '570'
department:
- _id: MiSi
doi: 10.21769/bioprotoc.3018
ec_funded: 1
file:
- access_level: open_access
  checksum: d4588377e789da7f360b553ae02c5119
  content_type: application/pdf
  creator: dernst
  date_created: 2019-04-30T08:04:33Z
  date_updated: 2020-07-14T12:47:28Z
  file_id: '6360'
  file_name: 2018_BioProtocol_Fan.pdf
  file_size: 2928337
  relation: main_file
file_date_updated: 2020-07-14T12:47:28Z
has_accepted_license: '1'
intvolume: '         8'
issue: '18'
keyword:
- Platelets
- Cell migration
- Bacteria
- Shear flow
- Fibrinogen
- E. coli
language:
- iso: eng
month: '09'
oa: 1
oa_version: Published Version
project:
- _id: 260AA4E2-B435-11E9-9278-68D0E5697425
  call_identifier: H2020
  grant_number: '747687'
  name: Mechanical Adaptation of Lamellipodial Actin Networks in Migrating Cells
publication: Bio-Protocol
publication_identifier:
  issn:
  - 2331-8325
publication_status: published
publisher: Bio-Protocol
quality_controlled: '1'
status: public
title: Platelet migration and bacterial trapping assay under flow
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
volume: 8
year: '2018'
...