[{"publication":"Frontiers in Oncology","language":[{"iso":"eng"}],"isi":1,"has_accepted_license":"1","intvolume":"        12","scopus_import":"1","article_processing_charge":"No","oa":1,"date_updated":"2023-08-04T09:54:16Z","title":"Substrate stiffness effect on molecular crosstalk of epithelial-mesenchymal transition mediators of human glioblastoma cells","type":"journal_article","status":"public","pmid":1,"publication_identifier":{"issn":["2234-943X"]},"month":"08","file":[{"file_name":"2022_FrontiersOntology_Basilico.pdf","checksum":"efc7edf9f626af31853790c5b598a68c","file_id":"12450","success":1,"date_updated":"2023-01-30T10:25:21Z","date_created":"2023-01-30T10:25:21Z","creator":"dernst","access_level":"open_access","content_type":"application/pdf","file_size":13588502,"relation":"main_file"}],"user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","oa_version":"Published Version","day":"25","author":[{"last_name":"Basilico","id":"36035796-5ACA-11E9-A75E-7AF2E5697425","orcid":"0000-0003-1843-3173","full_name":"Basilico, Bernadette","first_name":"Bernadette"},{"first_name":"Ilaria Elena","full_name":"Palamà, Ilaria Elena","last_name":"Palamà"},{"last_name":"D’Amone","first_name":"Stefania","full_name":"D’Amone, Stefania"},{"first_name":"Clotilde","full_name":"Lauro, Clotilde","last_name":"Lauro"},{"first_name":"Maria","full_name":"Rosito, Maria","last_name":"Rosito"},{"first_name":"Maddalena","full_name":"Grieco, Maddalena","last_name":"Grieco"},{"last_name":"Ratano","first_name":"Patrizia","full_name":"Ratano, Patrizia"},{"full_name":"Cordella, Federica","first_name":"Federica","last_name":"Cordella"},{"last_name":"Sanchini","full_name":"Sanchini, Caterina","first_name":"Caterina"},{"full_name":"Di Angelantonio, Silvia","first_name":"Silvia","last_name":"Di Angelantonio"},{"first_name":"Davide","full_name":"Ragozzino, Davide","last_name":"Ragozzino"},{"first_name":"Mariafrancesca","full_name":"Cascione, Mariafrancesca","last_name":"Cascione"},{"last_name":"Gigli","full_name":"Gigli, Giuseppe","first_name":"Giuseppe"},{"last_name":"Cortese","full_name":"Cortese, Barbara","first_name":"Barbara"}],"doi":"10.3389/fonc.2022.983507","_id":"12268","article_type":"original","file_date_updated":"2023-01-30T10:25:21Z","article_number":"983507","year":"2022","acknowledgement":"The research leading to these results has received funding from AIRC under IG 2021 - ID. 26328 project – P.I. Cortese Barbara and AIRC under MFAG 2015 - ID. 16803 project – “P.I. Cortese Barbara”. The authors are also grateful to the ”Tecnopolo per la medicina di precisione” (TecnoMed Puglia) - Regione Puglia: DGR n.2117 del 21/11/2018, CUP: B84I18000540002 and “Tecnopolo di Nanotecnologia e Fotonica per la medicina di precisione” (TECNOMED) - FISR/MIUR-CNR: delibera CIPE n.3449 del 7-08-2017, CUP: B83B17000010001.\r\nWe thank Dr. Francesca Pagani for useful technical support. We thank also Irene Iacuitto, Giovanna Loffredo and Manuela Marchetti for practical administrative support.","keyword":["Cancer Research","Oncology"],"citation":{"apa":"Basilico, B., Palamà, I. E., D’Amone, S., Lauro, C., Rosito, M., Grieco, M., … Cortese, B. (2022). Substrate stiffness effect on molecular crosstalk of epithelial-mesenchymal transition mediators of human glioblastoma cells. <i>Frontiers in Oncology</i>. Frontiers Media. <a href=\"https://doi.org/10.3389/fonc.2022.983507\">https://doi.org/10.3389/fonc.2022.983507</a>","short":"B. Basilico, I.E. Palamà, S. D’Amone, C. Lauro, M. Rosito, M. Grieco, P. Ratano, F. Cordella, C. Sanchini, S. Di Angelantonio, D. Ragozzino, M. Cascione, G. Gigli, B. Cortese, Frontiers in Oncology 12 (2022).","ama":"Basilico B, Palamà IE, D’Amone S, et al. Substrate stiffness effect on molecular crosstalk of epithelial-mesenchymal transition mediators of human glioblastoma cells. <i>Frontiers in Oncology</i>. 2022;12. doi:<a href=\"https://doi.org/10.3389/fonc.2022.983507\">10.3389/fonc.2022.983507</a>","ieee":"B. Basilico <i>et al.</i>, “Substrate stiffness effect on molecular crosstalk of epithelial-mesenchymal transition mediators of human glioblastoma cells,” <i>Frontiers in Oncology</i>, vol. 12. Frontiers Media, 2022.","mla":"Basilico, Bernadette, et al. “Substrate Stiffness Effect on Molecular Crosstalk of Epithelial-Mesenchymal Transition Mediators of Human Glioblastoma Cells.” <i>Frontiers in Oncology</i>, vol. 12, 983507, Frontiers Media, 2022, doi:<a href=\"https://doi.org/10.3389/fonc.2022.983507\">10.3389/fonc.2022.983507</a>.","ista":"Basilico B, Palamà IE, D’Amone S, Lauro C, Rosito M, Grieco M, Ratano P, Cordella F, Sanchini C, Di Angelantonio S, Ragozzino D, Cascione M, Gigli G, Cortese B. 2022. Substrate stiffness effect on molecular crosstalk of epithelial-mesenchymal transition mediators of human glioblastoma cells. Frontiers in Oncology. 12, 983507.","chicago":"Basilico, Bernadette, Ilaria Elena Palamà, Stefania D’Amone, Clotilde Lauro, Maria Rosito, Maddalena Grieco, Patrizia Ratano, et al. “Substrate Stiffness Effect on Molecular Crosstalk of Epithelial-Mesenchymal Transition Mediators of Human Glioblastoma Cells.” <i>Frontiers in Oncology</i>. Frontiers Media, 2022. <a href=\"https://doi.org/10.3389/fonc.2022.983507\">https://doi.org/10.3389/fonc.2022.983507</a>."},"date_created":"2023-01-16T10:00:28Z","date_published":"2022-08-25T00:00:00Z","publisher":"Frontiers Media","quality_controlled":"1","volume":12,"department":[{"_id":"GaNo"}],"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"ddc":["570"],"abstract":[{"text":"The complexity of the microenvironment effects on cell response, show accumulating evidence that glioblastoma (GBM) migration and invasiveness are influenced by the mechanical rigidity of their surroundings. The epithelial–mesenchymal transition (EMT) is a well-recognized driving force of the invasive behavior of cancer. However, the primary mechanisms of EMT initiation and progression remain unclear. We have previously showed that certain substrate stiffness can selectively stimulate human GBM U251-MG and GL15 glioblastoma cell lines motility. The present study unifies several known EMT mediators to uncover the reason of the regulation and response to these stiffnesses. Our results revealed that changing the rigidity of the mechanical environment tuned the response of both cell lines through change in morphological features, epithelial-mesenchymal markers (E-, N-Cadherin), EGFR and ROS expressions in an interrelated manner. Specifically, a stiffer microenvironment induced a mesenchymal cell shape, a more fragmented morphology, higher intracellular cytosolic ROS expression and lower mitochondrial ROS. Finally, we observed that cells more motile showed a more depolarized mitochondrial membrane potential. Unravelling the process that regulates GBM cells’ infiltrative behavior could provide new opportunities for identification of new targets and less invasive approaches for treatment.","lang":"eng"}],"external_id":{"isi":["000856524900001"],"pmid":["36091138"]},"publication_status":"published"},{"author":[{"last_name":"Christophorou","first_name":"Nicolas","full_name":"Christophorou, Nicolas"},{"first_name":"Wenjing","full_name":"She, Wenjing","last_name":"She"},{"last_name":"Long","first_name":"Jincheng","full_name":"Long, Jincheng"},{"last_name":"Hurel","full_name":"Hurel, Aurélie","first_name":"Aurélie"},{"first_name":"Sébastien","full_name":"Beaubiat, Sébastien","last_name":"Beaubiat"},{"last_name":"Idir","first_name":"Yassir","full_name":"Idir, Yassir"},{"last_name":"Tagliaro-Jahns","first_name":"Marina","full_name":"Tagliaro-Jahns, Marina"},{"last_name":"Chambon","first_name":"Aurélie","full_name":"Chambon, Aurélie"},{"last_name":"Solier","full_name":"Solier, Victor","first_name":"Victor"},{"last_name":"Vezon","first_name":"Daniel","full_name":"Vezon, Daniel"},{"full_name":"Grelon, Mathilde","first_name":"Mathilde","last_name":"Grelon"},{"last_name":"Feng","id":"e0164712-22ee-11ed-b12a-d80fcdf35958","orcid":"0000-0002-4008-1234","full_name":"Feng, Xiaoqi","first_name":"Xiaoqi"},{"full_name":"Bouché, Nicolas","first_name":"Nicolas","last_name":"Bouché"},{"last_name":"Mézard","full_name":"Mézard, Christine","first_name":"Christine"}],"publication_identifier":{"issn":["1553-7404"]},"month":"06","day":"29","oa_version":"Published Version","main_file_link":[{"open_access":"1","url":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7351236/"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","pmid":1,"status":"public","type":"journal_article","date_updated":"2023-05-08T10:54:39Z","oa":1,"article_processing_charge":"No","issue":"6","title":"AXR1 affects DNA methylation independently of its role in regulating meiotic crossover localization","scopus_import":"1","intvolume":"        16","language":[{"iso":"eng"}],"publication":"PLOS Genetics","publication_status":"published","external_id":{"pmid":["32598340"]},"abstract":[{"text":"Meiotic crossovers (COs) are important for reshuffling genetic information between homologous chromosomes and they are essential for their correct segregation. COs are unevenly distributed along chromosomes and the underlying mechanisms controlling CO localization are not well understood. We previously showed that meiotic COs are mis-localized in the absence of AXR1, an enzyme involved in the neddylation/rubylation protein modification pathway in Arabidopsis thaliana. Here, we report that in axr1-/-, male meiocytes show a strong defect in chromosome pairing whereas the formation of the telomere bouquet is not affected. COs are also redistributed towards subtelomeric chromosomal ends where they frequently form clusters, in contrast to large central regions depleted in recombination. The CO suppressed regions correlate with DNA hypermethylation of transposable elements (TEs) in the CHH context in axr1-/- meiocytes. Through examining somatic methylomes, we found axr1-/- affects DNA methylation in a plant, causing hypermethylation in all sequence contexts (CG, CHG and CHH) in TEs. Impairment of the main pathways involved in DNA methylation is epistatic over axr1-/- for DNA methylation in somatic cells but does not restore regular chromosome segregation during meiosis. Collectively, our findings reveal that the neddylation pathway not only regulates hormonal perception and CO distribution but is also, directly or indirectly, a major limiting pathway of TE DNA methylation in somatic cells.","lang":"eng"}],"publisher":"Public Library of Science (PLoS)","date_published":"2020-06-29T00:00:00Z","department":[{"_id":"XiFe"}],"extern":"1","quality_controlled":"1","volume":16,"acknowledgement":"The authors wish to thank Cécile Raynaud, Eric Jenczewski, Rajeev Kumar, Raphaël Mercier and Jean Molinier for critical reading of the manuscript.","year":"2020","date_created":"2023-01-16T09:16:10Z","citation":{"apa":"Christophorou, N., She, W., Long, J., Hurel, A., Beaubiat, S., Idir, Y., … Mézard, C. (2020). AXR1 affects DNA methylation independently of its role in regulating meiotic crossover localization. <i>PLOS Genetics</i>. Public Library of Science (PLoS). <a href=\"https://doi.org/10.1371/journal.pgen.1008894\">https://doi.org/10.1371/journal.pgen.1008894</a>","mla":"Christophorou, Nicolas, et al. “AXR1 Affects DNA Methylation Independently of Its Role in Regulating Meiotic Crossover Localization.” <i>PLOS Genetics</i>, vol. 16, no. 6, e1008894, Public Library of Science (PLoS), 2020, doi:<a href=\"https://doi.org/10.1371/journal.pgen.1008894\">10.1371/journal.pgen.1008894</a>.","chicago":"Christophorou, Nicolas, Wenjing She, Jincheng Long, Aurélie Hurel, Sébastien Beaubiat, Yassir Idir, Marina Tagliaro-Jahns, et al. “AXR1 Affects DNA Methylation Independently of Its Role in Regulating Meiotic Crossover Localization.” <i>PLOS Genetics</i>. Public Library of Science (PLoS), 2020. <a href=\"https://doi.org/10.1371/journal.pgen.1008894\">https://doi.org/10.1371/journal.pgen.1008894</a>.","ista":"Christophorou N, She W, Long J, Hurel A, Beaubiat S, Idir Y, Tagliaro-Jahns M, Chambon A, Solier V, Vezon D, Grelon M, Feng X, Bouché N, Mézard C. 2020. AXR1 affects DNA methylation independently of its role in regulating meiotic crossover localization. PLOS Genetics. 16(6), e1008894.","short":"N. Christophorou, W. She, J. Long, A. Hurel, S. Beaubiat, Y. Idir, M. Tagliaro-Jahns, A. Chambon, V. Solier, D. Vezon, M. Grelon, X. Feng, N. Bouché, C. Mézard, PLOS Genetics 16 (2020).","ama":"Christophorou N, She W, Long J, et al. AXR1 affects DNA methylation independently of its role in regulating meiotic crossover localization. <i>PLOS Genetics</i>. 2020;16(6). doi:<a href=\"https://doi.org/10.1371/journal.pgen.1008894\">10.1371/journal.pgen.1008894</a>","ieee":"N. Christophorou <i>et al.</i>, “AXR1 affects DNA methylation independently of its role in regulating meiotic crossover localization,” <i>PLOS Genetics</i>, vol. 16, no. 6. Public Library of Science (PLoS), 2020."},"keyword":["Cancer Research","Genetics (clinical)","Genetics","Molecular Biology","Ecology","Evolution","Behavior and Systematics"],"article_number":"e1008894","article_type":"original","_id":"12189","doi":"10.1371/journal.pgen.1008894"},{"pmid":1,"type":"journal_article","status":"public","publication_identifier":{"issn":["1553-7404"]},"month":"02","main_file_link":[{"url":"https://doi.org/10.1371/journal.pgen.1003308","open_access":"1"}],"oa_version":"Published Version","day":"28","user_id":"72615eeb-f1f3-11ec-aa25-d4573ddc34fd","author":[{"first_name":"Yun","full_name":"Liang, Yun","last_name":"Liang"},{"first_name":"Tobias M.","full_name":"Franks, Tobias M.","last_name":"Franks"},{"last_name":"Marchetto","full_name":"Marchetto, Maria C.","first_name":"Maria C."},{"full_name":"Gage, Fred H.","first_name":"Fred H.","last_name":"Gage"},{"orcid":"0000-0002-2111-992X","id":"86c0d31b-b4eb-11ec-ac5a-eae7b2e135ed","last_name":"HETZER","full_name":"HETZER, Martin W","first_name":"Martin W"}],"publication":"PLoS Genetics","language":[{"iso":"eng"}],"intvolume":"         9","scopus_import":"1","oa":1,"date_updated":"2022-07-18T08:45:58Z","issue":"2","article_processing_charge":"No","title":"Dynamic association of NUP98 with the human genome","publisher":"Public Library of Science","date_published":"2013-02-28T00:00:00Z","extern":"1","volume":9,"quality_controlled":"1","external_id":{"pmid":["23468646"]},"abstract":[{"lang":"eng","text":"Faithful execution of developmental gene expression programs occurs at multiple levels and involves many different components such as transcription factors, histone-modification enzymes, and mRNA processing proteins. Recent evidence suggests that nucleoporins, well known components that control nucleo-cytoplasmic trafficking, have wide-ranging functions in developmental gene regulation that potentially extend beyond their role in nuclear transport. Whether the unexpected role of nuclear pore proteins in transcription regulation, which initially has been described in fungi and flies, also applies to human cells is unknown. Here we show at a genome-wide level that the nuclear pore protein NUP98 associates with developmentally regulated genes active during human embryonic stem cell differentiation. Overexpression of a dominant negative fragment of NUP98 levels decreases expression levels of NUP98-bound genes. In addition, we identify two modes of developmental gene regulation by NUP98 that are differentiated by the spatial localization of NUP98 target genes. Genes in the initial stage of developmental induction can associate with NUP98 that is embedded in the nuclear pores at the nuclear periphery. Alternatively, genes that are highly induced can interact with NUP98 in the nuclear interior, away from the nuclear pores. This work demonstrates for the first time that NUP98 dynamically associates with the human genome during differentiation, revealing a role of a nuclear pore protein in regulating developmental gene expression programs."}],"publication_status":"published","doi":"10.1371/journal.pgen.1003308","_id":"11086","article_type":"original","article_number":"e1003308","year":"2013","citation":{"apa":"Liang, Y., Franks, T. M., Marchetto, M. C., Gage, F. H., &#38; Hetzer, M. (2013). Dynamic association of NUP98 with the human genome. <i>PLoS Genetics</i>. Public Library of Science. <a href=\"https://doi.org/10.1371/journal.pgen.1003308\">https://doi.org/10.1371/journal.pgen.1003308</a>","short":"Y. Liang, T.M. Franks, M.C. Marchetto, F.H. Gage, M. Hetzer, PLoS Genetics 9 (2013).","ieee":"Y. Liang, T. M. Franks, M. C. Marchetto, F. H. Gage, and M. Hetzer, “Dynamic association of NUP98 with the human genome,” <i>PLoS Genetics</i>, vol. 9, no. 2. Public Library of Science, 2013.","ama":"Liang Y, Franks TM, Marchetto MC, Gage FH, Hetzer M. Dynamic association of NUP98 with the human genome. <i>PLoS Genetics</i>. 2013;9(2). doi:<a href=\"https://doi.org/10.1371/journal.pgen.1003308\">10.1371/journal.pgen.1003308</a>","mla":"Liang, Yun, et al. “Dynamic Association of NUP98 with the Human Genome.” <i>PLoS Genetics</i>, vol. 9, no. 2, e1003308, Public Library of Science, 2013, doi:<a href=\"https://doi.org/10.1371/journal.pgen.1003308\">10.1371/journal.pgen.1003308</a>.","chicago":"Liang, Yun, Tobias M. Franks, Maria C. Marchetto, Fred H. Gage, and Martin Hetzer. “Dynamic Association of NUP98 with the Human Genome.” <i>PLoS Genetics</i>. Public Library of Science, 2013. <a href=\"https://doi.org/10.1371/journal.pgen.1003308\">https://doi.org/10.1371/journal.pgen.1003308</a>.","ista":"Liang Y, Franks TM, Marchetto MC, Gage FH, Hetzer M. 2013. Dynamic association of NUP98 with the human genome. PLoS Genetics. 9(2), e1003308."},"date_created":"2022-04-07T07:50:59Z","keyword":["Cancer Research","Genetics (clinical)","Genetics","Molecular Biology","Ecology","Evolution","Behavior and Systematics"]}]