---
_id: '14286'
abstract:
- lang: eng
  text: 'The bacteriophage M13 has found frequent applications in nanobiotechnology
    due to its chemically and genetically tunable protein surface and its ability
    to self-assemble into colloidal membranes. Additionally, its single-stranded (ss)
    genome is commonly used as scaffold for DNA origami. Despite the manifold uses
    of M13, upstream production methods for phage and scaffold ssDNA are underexamined
    with respect to future industrial usage. Here, the high-cell-density phage production
    with Escherichia coli as host organism was studied in respect of medium composition,
    infection time, multiplicity of infection, and specific growth rate. The specific
    growth rate and the multiplicity of infection were identified as the crucial state
    variables that influence phage amplification rate on one hand and the concentration
    of produced ssDNA on the other hand. Using a growth rate of 0.15 h−1 and a multiplicity
    of infection of 0.05 pfu cfu−1 in the fed-batch production process, the concentration
    of pure isolated M13 ssDNA usable for scaffolded DNA origami could be enhanced
    by 54% to 590 mg L−1. Thus, our results help enabling M13 production for industrial
    uses in nanobiotechnology. Biotechnol. Bioeng. 2017;114: 777–784.'
article_processing_charge: No
article_type: original
author:
- first_name: Benjamin
  full_name: Kick, Benjamin
  last_name: Kick
- first_name: Samantha
  full_name: Hensler, Samantha
  last_name: Hensler
- first_name: Florian M
  full_name: Praetorius, Florian M
  id: dfec9381-4341-11ee-8fd8-faa02bba7d62
  last_name: Praetorius
- first_name: Hendrik
  full_name: Dietz, Hendrik
  last_name: Dietz
- first_name: Dirk
  full_name: Weuster-Botz, Dirk
  last_name: Weuster-Botz
citation:
  ama: Kick B, Hensler S, Praetorius FM, Dietz H, Weuster-Botz D. Specific growth
    rate and multiplicity of infection affect high-cell-density fermentation with
    bacteriophage M13 for ssDNA production. <i>Biotechnology and Bioengineering</i>.
    2017;114(4):777-784. doi:<a href="https://doi.org/10.1002/bit.26200">10.1002/bit.26200</a>
  apa: Kick, B., Hensler, S., Praetorius, F. M., Dietz, H., &#38; Weuster-Botz, D.
    (2017). Specific growth rate and multiplicity of infection affect high-cell-density
    fermentation with bacteriophage M13 for ssDNA production. <i>Biotechnology and
    Bioengineering</i>. Wiley. <a href="https://doi.org/10.1002/bit.26200">https://doi.org/10.1002/bit.26200</a>
  chicago: Kick, Benjamin, Samantha Hensler, Florian M Praetorius, Hendrik Dietz,
    and Dirk Weuster-Botz. “Specific Growth Rate and Multiplicity of Infection Affect
    High-Cell-Density Fermentation with Bacteriophage M13 for SsDNA Production.” <i>Biotechnology
    and Bioengineering</i>. Wiley, 2017. <a href="https://doi.org/10.1002/bit.26200">https://doi.org/10.1002/bit.26200</a>.
  ieee: B. Kick, S. Hensler, F. M. Praetorius, H. Dietz, and D. Weuster-Botz, “Specific
    growth rate and multiplicity of infection affect high-cell-density fermentation
    with bacteriophage M13 for ssDNA production,” <i>Biotechnology and Bioengineering</i>,
    vol. 114, no. 4. Wiley, pp. 777–784, 2017.
  ista: Kick B, Hensler S, Praetorius FM, Dietz H, Weuster-Botz D. 2017. Specific
    growth rate and multiplicity of infection affect high-cell-density fermentation
    with bacteriophage M13 for ssDNA production. Biotechnology and Bioengineering.
    114(4), 777–784.
  mla: Kick, Benjamin, et al. “Specific Growth Rate and Multiplicity of Infection
    Affect High-Cell-Density Fermentation with Bacteriophage M13 for SsDNA Production.”
    <i>Biotechnology and Bioengineering</i>, vol. 114, no. 4, Wiley, 2017, pp. 777–84,
    doi:<a href="https://doi.org/10.1002/bit.26200">10.1002/bit.26200</a>.
  short: B. Kick, S. Hensler, F.M. Praetorius, H. Dietz, D. Weuster-Botz, Biotechnology
    and Bioengineering 114 (2017) 777–784.
date_created: 2023-09-06T12:08:29Z
date_published: 2017-04-01T00:00:00Z
date_updated: 2023-11-07T12:36:20Z
day: '01'
doi: 10.1002/bit.26200
extern: '1'
external_id:
  pmid:
  - '27748519'
intvolume: '       114'
issue: '4'
keyword:
- Applied Microbiology and Biotechnology
- Bioengineering
- Biotechnology
language:
- iso: eng
month: '04'
oa_version: None
page: 777-784
pmid: 1
publication: Biotechnology and Bioengineering
publication_identifier:
  issn:
  - 0006-3592
publication_status: published
publisher: Wiley
quality_controlled: '1'
scopus_import: '1'
status: public
title: Specific growth rate and multiplicity of infection affect high-cell-density
  fermentation with bacteriophage M13 for ssDNA production
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 114
year: '2017'
...