---
_id: '10095'
abstract:
- lang: eng
  text: Growth regulation tailors plant development to its environment. A showcase
    is response to gravity, where shoots bend up and roots down1. This paradox is
    based on opposite effects of the phytohormone auxin, which promotes cell expansion
    in shoots, while inhibiting it in roots via a yet unknown cellular mechanism2.
    Here, by combining microfluidics, live imaging, genetic engineering and phospho-proteomics
    in Arabidopsis thaliana, we advance our understanding how auxin inhibits root
    growth. We show that auxin activates two distinct, antagonistically acting signalling
    pathways that converge on the rapid regulation of the apoplastic pH, a causative
    growth determinant. Cell surface-based TRANSMEMBRANE KINASE1 (TMK1) interacts
    with and mediates phosphorylation and activation of plasma membrane H+-ATPases
    for apoplast acidification, while intracellular canonical auxin signalling promotes
    net cellular H+-influx, causing apoplast alkalinisation. The simultaneous activation
    of these two counteracting mechanisms poises the root for a rapid, fine-tuned
    growth modulation while navigating complex soil environment.
acknowledged_ssus:
- _id: LifeSc
- _id: M-Shop
- _id: Bio
acknowledgement: We thank Nataliia Gnyliukh and Lukas Hörmayer for technical assistance
  and Nadine Paris for sharing PM-Cyto seeds. We gratefully acknowledge Life Science,
  Machine Shop and Bioimaging Facilities of IST Austria. This project has received
  funding from the European Research Council Advanced Grant (ETAP-742985) and the
  Austrian Science Fund (FWF) I 3630-B25 to J.F., the National Institutes of Health
  (GM067203) to W.M.G., the Netherlands Organization for Scientific Research (NWO;
  VIDI-864.13.001.), the Research Foundation-Flanders (FWO; Odysseus II G0D0515N)
  and a European Research Council Starting Grant (TORPEDO-714055) to W.S. and B.D.R.,
  the VICI grant (865.14.001) from the Netherlands Organization for Scientific Research
  to M.R and D.W., the Australian Research Council and China National Distinguished
  Expert Project (WQ20174400441) to S.S., the MEXT/JSPS KAKENHI to K.T. (20K06685)
  and T.K. (20H05687 and 20H05910),  the European Union’s Horizon 2020 research and
  innovation programme under the Marie Skłodowska-Curie Grant Agreement No. 665385
  and the DOC Fellowship of the Austrian Academy of Sciences to L.L., the China Scholarship
  Council to J.C.
article_number: '266395'
article_processing_charge: No
author:
- first_name: Lanxin
  full_name: Li, Lanxin
  id: 367EF8FA-F248-11E8-B48F-1D18A9856A87
  last_name: Li
  orcid: 0000-0002-5607-272X
- first_name: Inge
  full_name: Verstraeten, Inge
  id: 362BF7FE-F248-11E8-B48F-1D18A9856A87
  last_name: Verstraeten
  orcid: 0000-0001-7241-2328
- first_name: Mark
  full_name: Roosjen, Mark
  last_name: Roosjen
- first_name: Koji
  full_name: Takahashi, Koji
  last_name: Takahashi
- first_name: Lesia
  full_name: Rodriguez Solovey, Lesia
  id: 3922B506-F248-11E8-B48F-1D18A9856A87
  last_name: Rodriguez Solovey
  orcid: 0000-0002-7244-7237
- first_name: Jack
  full_name: Merrin, Jack
  id: 4515C308-F248-11E8-B48F-1D18A9856A87
  last_name: Merrin
  orcid: 0000-0001-5145-4609
- first_name: Jian
  full_name: Chen, Jian
  last_name: Chen
- first_name: Lana
  full_name: Shabala, Lana
  last_name: Shabala
- first_name: Wouter
  full_name: Smet, Wouter
  last_name: Smet
- first_name: Hong
  full_name: Ren, Hong
  last_name: Ren
- first_name: Steffen
  full_name: Vanneste, Steffen
  last_name: Vanneste
- first_name: Sergey
  full_name: Shabala, Sergey
  last_name: Shabala
- first_name: Bert
  full_name: De Rybel, Bert
  last_name: De Rybel
- first_name: Dolf
  full_name: Weijers, Dolf
  last_name: Weijers
- first_name: Toshinori
  full_name: Kinoshita, Toshinori
  last_name: Kinoshita
- first_name: William M.
  full_name: Gray, William M.
  last_name: Gray
- first_name: Jiří
  full_name: Friml, Jiří
  id: 4159519E-F248-11E8-B48F-1D18A9856A87
  last_name: Friml
  orcid: 0000-0002-8302-7596
citation:
  ama: Li L, Verstraeten I, Roosjen M, et al. Cell surface and intracellular auxin
    signalling for H+-fluxes in root growth. <i>Research Square</i>. doi:<a href="https://doi.org/10.21203/rs.3.rs-266395/v3">10.21203/rs.3.rs-266395/v3</a>
  apa: Li, L., Verstraeten, I., Roosjen, M., Takahashi, K., Rodriguez Solovey, L.,
    Merrin, J., … Friml, J. (n.d.). Cell surface and intracellular auxin signalling
    for H+-fluxes in root growth. <i>Research Square</i>. <a href="https://doi.org/10.21203/rs.3.rs-266395/v3">https://doi.org/10.21203/rs.3.rs-266395/v3</a>
  chicago: Li, Lanxin, Inge Verstraeten, Mark Roosjen, Koji Takahashi, Lesia Rodriguez
    Solovey, Jack Merrin, Jian Chen, et al. “Cell Surface and Intracellular Auxin
    Signalling for H+-Fluxes in Root Growth.” <i>Research Square</i>, n.d. <a href="https://doi.org/10.21203/rs.3.rs-266395/v3">https://doi.org/10.21203/rs.3.rs-266395/v3</a>.
  ieee: L. Li <i>et al.</i>, “Cell surface and intracellular auxin signalling for
    H+-fluxes in root growth,” <i>Research Square</i>. .
  ista: Li L, Verstraeten I, Roosjen M, Takahashi K, Rodriguez Solovey L, Merrin J,
    Chen J, Shabala L, Smet W, Ren H, Vanneste S, Shabala S, De Rybel B, Weijers D,
    Kinoshita T, Gray WM, Friml J. Cell surface and intracellular auxin signalling
    for H+-fluxes in root growth. Research Square, 266395.
  mla: Li, Lanxin, et al. “Cell Surface and Intracellular Auxin Signalling for H+-Fluxes
    in Root Growth.” <i>Research Square</i>, 266395, doi:<a href="https://doi.org/10.21203/rs.3.rs-266395/v3">10.21203/rs.3.rs-266395/v3</a>.
  short: L. Li, I. Verstraeten, M. Roosjen, K. Takahashi, L. Rodriguez Solovey, J.
    Merrin, J. Chen, L. Shabala, W. Smet, H. Ren, S. Vanneste, S. Shabala, B. De Rybel,
    D. Weijers, T. Kinoshita, W.M. Gray, J. Friml, Research Square (n.d.).
date_created: 2021-10-06T08:56:22Z
date_published: 2021-09-09T00:00:00Z
date_updated: 2024-10-29T10:22:44Z
day: '09'
department:
- _id: JiFr
- _id: NanoFab
doi: 10.21203/rs.3.rs-266395/v3
ec_funded: 1
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: https://www.doi.org/10.21203/rs.3.rs-266395/v3
month: '09'
oa: 1
oa_version: Preprint
project:
- _id: 2564DBCA-B435-11E9-9278-68D0E5697425
  call_identifier: H2020
  grant_number: '665385'
  name: International IST Doctoral Program
- _id: 261099A6-B435-11E9-9278-68D0E5697425
  call_identifier: H2020
  grant_number: '742985'
  name: Tracing Evolution of Auxin Transport and Polarity in Plants
- _id: 26538374-B435-11E9-9278-68D0E5697425
  call_identifier: FWF
  grant_number: I03630
  name: Molecular mechanisms of endocytic cargo recognition in plants
- _id: 26B4D67E-B435-11E9-9278-68D0E5697425
  grant_number: '25351'
  name: 'A Case Study of Plant Growth Regulation: Molecular Mechanism of Auxin-mediated
    Rapid Growth Inhibition in Arabidopsis Root'
publication: Research Square
publication_identifier:
  issn:
  - 2693-5015
publication_status: accepted
related_material:
  record:
  - id: '10083'
    relation: dissertation_contains
    status: public
  - id: '10223'
    relation: later_version
    status: public
status: public
title: Cell surface and intracellular auxin signalling for H+-fluxes in root growth
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: preprint
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '2021'
...