---
_id: '14251'
abstract:
- lang: eng
  text: The phytohormone auxin and its directional transport through tissues play
    a fundamental role in development of higher plants. This polar auxin transport
    predominantly relies on PIN-FORMED (PIN) auxin exporters. Hence, PIN polarization
    is crucial for development, but its evolution during the rise of morphological
    complexity in land plants remains unclear. Here, we performed a cross-species
    investigation by observing the trafficking and localization of endogenous and
    exogenous PINs in two bryophytes, Physcomitrium patens and Marchantia polymorpha,
    and in the flowering plant Arabidopsis thaliana. We confirmed that the GFP fusion
    did not compromise the auxin export function of all examined PINs by using radioactive
    auxin export assay and by observing the phenotypic changes in transgenic bryophytes.
    Endogenous PINs polarize to filamentous apices, while exogenous Arabidopsis PINs
    distribute symmetrically on the membrane in both bryophytes. In Arabidopsis root
    epidermis, bryophytic PINs show no defined polarity. Pharmacological interference
    revealed a strong cytoskeleton dependence of bryophytic but not Arabidopsis PIN
    polarization. The divergence of PIN polarization and trafficking is also observed
    within the bryophyte clade and between tissues of individual species. These results
    collectively reveal a divergence of PIN trafficking and polarity mechanisms throughout
    land plant evolution and a co-evolution of PIN sequence-based and cell-based polarity
    mechanisms.
acknowledgement: This work was supported by the ERC grant (PR1023ERC02) to H. T. and
  J. F., and by the ministry of science and technology (grant number 110-2636-B-005-001)
  to K. J. L.
article_number: '100669'
article_processing_charge: Yes
article_type: original
author:
- first_name: Han
  full_name: Tang, Han
  id: 19BDF720-25A0-11EA-AC6E-928F3DDC885E
  last_name: Tang
  orcid: 0000-0001-6152-6637
- first_name: KJ
  full_name: Lu, KJ
  last_name: Lu
- first_name: Y
  full_name: Zhang, Y
  last_name: Zhang
- first_name: YL
  full_name: Cheng, YL
  last_name: Cheng
- first_name: SL
  full_name: Tu, SL
  last_name: Tu
- first_name: Jiří
  full_name: Friml, Jiří
  id: 4159519E-F248-11E8-B48F-1D18A9856A87
  last_name: Friml
  orcid: 0000-0002-8302-7596
citation:
  ama: Tang H, Lu K, Zhang Y, Cheng Y, Tu S, Friml J. Divergence of trafficking and
    polarization mechanisms for PIN auxin transporters during land plant evolution.
    <i>Plant Communications</i>. 2024;5(1). doi:<a href="https://doi.org/10.1016/j.xplc.2023.100669">10.1016/j.xplc.2023.100669</a>
  apa: Tang, H., Lu, K., Zhang, Y., Cheng, Y., Tu, S., &#38; Friml, J. (2024). Divergence
    of trafficking and polarization mechanisms for PIN auxin transporters during land
    plant evolution. <i>Plant Communications</i>. Elsevier. <a href="https://doi.org/10.1016/j.xplc.2023.100669">https://doi.org/10.1016/j.xplc.2023.100669</a>
  chicago: Tang, Han, KJ Lu, Y Zhang, YL Cheng, SL Tu, and Jiří Friml. “Divergence
    of Trafficking and Polarization Mechanisms for PIN Auxin Transporters during Land
    Plant Evolution.” <i>Plant Communications</i>. Elsevier, 2024. <a href="https://doi.org/10.1016/j.xplc.2023.100669">https://doi.org/10.1016/j.xplc.2023.100669</a>.
  ieee: H. Tang, K. Lu, Y. Zhang, Y. Cheng, S. Tu, and J. Friml, “Divergence of trafficking
    and polarization mechanisms for PIN auxin transporters during land plant evolution,”
    <i>Plant Communications</i>, vol. 5, no. 1. Elsevier, 2024.
  ista: Tang H, Lu K, Zhang Y, Cheng Y, Tu S, Friml J. 2024. Divergence of trafficking
    and polarization mechanisms for PIN auxin transporters during land plant evolution.
    Plant Communications. 5(1), 100669.
  mla: Tang, Han, et al. “Divergence of Trafficking and Polarization Mechanisms for
    PIN Auxin Transporters during Land Plant Evolution.” <i>Plant Communications</i>,
    vol. 5, no. 1, 100669, Elsevier, 2024, doi:<a href="https://doi.org/10.1016/j.xplc.2023.100669">10.1016/j.xplc.2023.100669</a>.
  short: H. Tang, K. Lu, Y. Zhang, Y. Cheng, S. Tu, J. Friml, Plant Communications
    5 (2024).
date_created: 2023-09-01T11:32:02Z
date_published: 2024-01-08T00:00:00Z
date_updated: 2025-07-02T12:51:02Z
day: '08'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1016/j.xplc.2023.100669
ec_funded: 1
external_id:
  pmid:
  - '37528584'
file:
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  date_created: 2024-01-30T12:59:57Z
  date_updated: 2024-01-30T12:59:57Z
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has_accepted_license: '1'
intvolume: '         5'
issue: '1'
language:
- iso: eng
license: https://creativecommons.org/licenses/by/4.0/
month: '01'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
  call_identifier: H2020
  grant_number: '742985'
  name: Tracing Evolution of Auxin Transport and Polarity in Plants
publication: Plant Communications
publication_identifier:
  issn:
  - 2590-3462
  issnl:
  - 1234-4567
publication_status: published
publisher: Elsevier
quality_controlled: '1'
scopus_import: '1'
status: public
title: Divergence of trafficking and polarization mechanisms for PIN auxin transporters
  during land plant evolution
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
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  short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 5
year: '2024'
...
---
_id: '9160'
abstract:
- lang: eng
  text: Auxin is a key hormonal regulator, that governs plant growth and development
    in concert with other hormonal pathways. The unique feature of auxin is its polar,
    cell-to-cell transport that leads to the formation of local auxin maxima and gradients,
    which coordinate initiation and patterning of plant organs. The molecular machinery
    mediating polar auxin transport is one of the important points of interaction
    with other hormones. Multiple hormonal pathways converge at the regulation of
    auxin transport and form a regulatory network that integrates various developmental
    and environmental inputs to steer plant development. In this review, we discuss
    recent advances in understanding the mechanisms that underlie regulation of polar
    auxin transport by multiple hormonal pathways. Specifically, we focus on the post-translational
    mechanisms that contribute to fine-tuning of the abundance and polarity of auxin
    transporters at the plasma membrane and thereby enable rapid modification of the
    auxin flow to coordinate plant growth and development.
acknowledgement: H.S. is the recipient of a DOC Fellowship of the Austrian Academy
  of Sciences at the Institute of Science and Technology, Austria. J.C.M. is the recipient
  of an EMBO Long-Term Fellowship (ALTF number 710-2016). We would like to thank Jiri
  Friml and Carina Baskett for critical reading of the manuscript and Shutang Tan
  and Maciek Adamowski for helpful discussions. No conflict of interest declared.
article_number: '100048'
article_processing_charge: No
article_type: original
author:
- first_name: Hana
  full_name: Semeradova, Hana
  id: 42FE702E-F248-11E8-B48F-1D18A9856A87
  last_name: Semeradova
- first_name: Juan C
  full_name: Montesinos López, Juan C
  id: 310A8E3E-F248-11E8-B48F-1D18A9856A87
  last_name: Montesinos López
  orcid: 0000-0001-9179-6099
- first_name: Eva
  full_name: Benková, Eva
  id: 38F4F166-F248-11E8-B48F-1D18A9856A87
  last_name: Benková
  orcid: 0000-0002-8510-9739
citation:
  ama: 'Semerádová H, Montesinos López JC, Benková E. All roads lead to auxin: Post-translational
    regulation of auxin transport by multiple hormonal pathways. <i>Plant Communications</i>.
    2020;1(3). doi:<a href="https://doi.org/10.1016/j.xplc.2020.100048">10.1016/j.xplc.2020.100048</a>'
  apa: 'Semerádová, H., Montesinos López, J. C., &#38; Benková, E. (2020). All roads
    lead to auxin: Post-translational regulation of auxin transport by multiple hormonal
    pathways. <i>Plant Communications</i>. Elsevier. <a href="https://doi.org/10.1016/j.xplc.2020.100048">https://doi.org/10.1016/j.xplc.2020.100048</a>'
  chicago: 'Semerádová, Hana, Juan C Montesinos López, and Eva Benková. “All Roads
    Lead to Auxin: Post-Translational Regulation of Auxin Transport by Multiple Hormonal
    Pathways.” <i>Plant Communications</i>. Elsevier, 2020. <a href="https://doi.org/10.1016/j.xplc.2020.100048">https://doi.org/10.1016/j.xplc.2020.100048</a>.'
  ieee: 'H. Semerádová, J. C. Montesinos López, and E. Benková, “All roads lead to
    auxin: Post-translational regulation of auxin transport by multiple hormonal pathways,”
    <i>Plant Communications</i>, vol. 1, no. 3. Elsevier, 2020.'
  ista: 'Semerádová H, Montesinos López JC, Benková E. 2020. All roads lead to auxin:
    Post-translational regulation of auxin transport by multiple hormonal pathways.
    Plant Communications. 1(3), 100048.'
  mla: 'Semerádová, Hana, et al. “All Roads Lead to Auxin: Post-Translational Regulation
    of Auxin Transport by Multiple Hormonal Pathways.” <i>Plant Communications</i>,
    vol. 1, no. 3, 100048, Elsevier, 2020, doi:<a href="https://doi.org/10.1016/j.xplc.2020.100048">10.1016/j.xplc.2020.100048</a>.'
  short: H. Semerádová, J.C. Montesinos López, E. Benková, Plant Communications 1
    (2020).
date_created: 2021-02-18T10:18:43Z
date_published: 2020-05-11T00:00:00Z
date_updated: 2024-03-25T23:30:26Z
day: '11'
ddc:
- '580'
department:
- _id: EvBe
doi: 10.1016/j.xplc.2020.100048
external_id:
  isi:
  - '000654052800010'
  pmid:
  - '33367243'
file:
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  checksum: 785b266d82a94b007cf40dbbe7c4847e
  content_type: application/pdf
  creator: dernst
  date_created: 2021-02-18T10:23:59Z
  date_updated: 2021-02-18T10:23:59Z
  file_id: '9161'
  file_name: 2020_PlantComm_Semeradova.pdf
  file_size: 840289
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file_date_updated: 2021-02-18T10:23:59Z
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issue: '3'
language:
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license: https://creativecommons.org/licenses/by-nc-nd/4.0/
month: '05'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 261821BC-B435-11E9-9278-68D0E5697425
  grant_number: '24746'
  name: Molecular mechanisms of the cytokinin regulated endomembrane trafficking to
    coordinate plant organogenesis.
- _id: 253E54C8-B435-11E9-9278-68D0E5697425
  grant_number: ALTF710-2016
  name: Molecular mechanism of auxindriven formative divisions delineating lateral
    root organogenesis in plants
publication: Plant Communications
publication_identifier:
  issn:
  - 2590-3462
publication_status: published
publisher: Elsevier
quality_controlled: '1'
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scopus_import: '1'
status: public
title: 'All roads lead to auxin: Post-translational regulation of auxin transport
  by multiple hormonal pathways'
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...