---
_id: '666'
abstract:
- lang: eng
  text: Antibiotics elicit drastic changes in microbial gene expression, including
    the induction of stress response genes. While certain stress responses are known
    to “cross-protect” bacteria from other stressors, it is unclear whether cellular
    responses to antibiotics have a similar protective role. By measuring the genome-wide
    transcriptional response dynamics of Escherichia coli to four antibiotics, we
    found that trimethoprim induces a rapid acid stress response that protects bacteria
    from subsequent exposure to acid. Combining microfluidics with time-lapse imaging
    to monitor survival and acid stress response in single cells revealed that the
    noisy expression of the acid resistance operon gadBC correlates with single-cell
    survival. Cells with higher gadBC expression following trimethoprim maintain higher
    intracellular pH and survive the acid stress longer. The seemingly random single-cell
    survival under acid stress can therefore be predicted from gadBC expression and
    rationalized in terms of GadB/C molecular function. Overall, we provide a roadmap
    for identifying the molecular mechanisms of single-cell cross-protection between
    antibiotics and other stressors.
article_processing_charge: Yes (in subscription journal)
author:
- first_name: Karin
  full_name: Mitosch, Karin
  id: 39B66846-F248-11E8-B48F-1D18A9856A87
  last_name: Mitosch
- first_name: Georg
  full_name: Rieckh, Georg
  id: 34DA8BD6-F248-11E8-B48F-1D18A9856A87
  last_name: Rieckh
- first_name: Tobias
  full_name: Bollenbach, Tobias
  id: 3E6DB97A-F248-11E8-B48F-1D18A9856A87
  last_name: Bollenbach
  orcid: 0000-0003-4398-476X
citation:
  ama: Mitosch K, Rieckh G, Bollenbach MT. Noisy response to antibiotic stress predicts
    subsequent single cell survival in an acidic environment. <i>Cell Systems</i>.
    2017;4(4):393-403. doi:<a href="https://doi.org/10.1016/j.cels.2017.03.001">10.1016/j.cels.2017.03.001</a>
  apa: Mitosch, K., Rieckh, G., &#38; Bollenbach, M. T. (2017). Noisy response to
    antibiotic stress predicts subsequent single cell survival in an acidic environment.
    <i>Cell Systems</i>. Cell Press. <a href="https://doi.org/10.1016/j.cels.2017.03.001">https://doi.org/10.1016/j.cels.2017.03.001</a>
  chicago: Mitosch, Karin, Georg Rieckh, and Mark Tobias Bollenbach. “Noisy Response
    to Antibiotic Stress Predicts Subsequent Single Cell Survival in an Acidic Environment.”
    <i>Cell Systems</i>. Cell Press, 2017. <a href="https://doi.org/10.1016/j.cels.2017.03.001">https://doi.org/10.1016/j.cels.2017.03.001</a>.
  ieee: K. Mitosch, G. Rieckh, and M. T. Bollenbach, “Noisy response to antibiotic
    stress predicts subsequent single cell survival in an acidic environment,” <i>Cell
    Systems</i>, vol. 4, no. 4. Cell Press, pp. 393–403, 2017.
  ista: Mitosch K, Rieckh G, Bollenbach MT. 2017. Noisy response to antibiotic stress
    predicts subsequent single cell survival in an acidic environment. Cell Systems.
    4(4), 393–403.
  mla: Mitosch, Karin, et al. “Noisy Response to Antibiotic Stress Predicts Subsequent
    Single Cell Survival in an Acidic Environment.” <i>Cell Systems</i>, vol. 4, no.
    4, Cell Press, 2017, pp. 393–403, doi:<a href="https://doi.org/10.1016/j.cels.2017.03.001">10.1016/j.cels.2017.03.001</a>.
  short: K. Mitosch, G. Rieckh, M.T. Bollenbach, Cell Systems 4 (2017) 393–403.
date_created: 2018-12-11T11:47:48Z
date_published: 2017-04-26T00:00:00Z
date_updated: 2023-09-07T12:00:25Z
day: '26'
ddc:
- '576'
- '610'
department:
- _id: ToBo
- _id: GaTk
doi: 10.1016/j.cels.2017.03.001
ec_funded: 1
file:
- access_level: open_access
  checksum: 04ff20011c3d9a601c514aa999a5fe1a
  content_type: application/pdf
  creator: system
  date_created: 2018-12-12T10:13:54Z
  date_updated: 2020-07-14T12:47:35Z
  file_id: '5041'
  file_name: IST-2017-901-v1+1_1-s2.0-S2405471217300868-main.pdf
  file_size: 2438660
  relation: main_file
file_date_updated: 2020-07-14T12:47:35Z
has_accepted_license: '1'
intvolume: '         4'
issue: '4'
language:
- iso: eng
month: '04'
oa: 1
oa_version: Published Version
page: 393 - 403
project:
- _id: 25E83C2C-B435-11E9-9278-68D0E5697425
  call_identifier: FP7
  grant_number: '303507'
  name: Optimality principles in responses to antibiotics
- _id: 25E9AF9E-B435-11E9-9278-68D0E5697425
  call_identifier: FWF
  grant_number: P27201-B22
  name: Revealing the mechanisms underlying drug interactions
- _id: 25EB3A80-B435-11E9-9278-68D0E5697425
  grant_number: RGP0042/2013
  name: Revealing the fundamental limits of cell growth
publication: Cell Systems
publication_identifier:
  issn:
  - '24054712'
publication_status: published
publisher: Cell Press
publist_id: '7061'
pubrep_id: '901'
quality_controlled: '1'
related_material:
  record:
  - id: '818'
    relation: dissertation_contains
    status: public
scopus_import: 1
status: public
title: Noisy response to antibiotic stress predicts subsequent single cell survival
  in an acidic environment
tmp:
  image: /images/cc_by_nc_nd.png
  legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
  name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
    (CC BY-NC-ND 4.0)
  short: CC BY-NC-ND (4.0)
type: journal_article
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
volume: 4
year: '2017'
...