[{"status":"public","intvolume":"         9","type":"journal_article","day":"25","file_date_updated":"2024-01-16T09:35:28Z","publication":"Science Advances","issue":"34","language":[{"iso":"eng"}],"publisher":"American Association for the Advancement of Science","date_published":"2023-08-25T00:00:00Z","article_type":"original","month":"08","date_created":"2024-01-10T09:48:01Z","file":[{"relation":"main_file","content_type":"application/pdf","file_id":"14809","creator":"dernst","success":1,"access_level":"open_access","date_updated":"2024-01-16T09:35:28Z","file_size":1596639,"file_name":"2023_ScienceAdvances_GallardoDodd.pdf","checksum":"b9072e20e2d5d9d34d2c53319bafee41","date_created":"2024-01-16T09:35:28Z"}],"department":[{"_id":"FlSc"}],"has_accepted_license":"1","keyword":["Multidisciplinary"],"author":[{"first_name":"Carlos J.","full_name":"Gallardo-Dodd, Carlos J.","last_name":"Gallardo-Dodd"},{"full_name":"Oertlin, Christian","last_name":"Oertlin","first_name":"Christian"},{"first_name":"Julien","last_name":"Record","full_name":"Record, Julien"},{"last_name":"Galvani","full_name":"Galvani, Rômulo G.","first_name":"Rômulo G."},{"first_name":"Christian","full_name":"Sommerauer, Christian","last_name":"Sommerauer"},{"first_name":"Nikolai V.","full_name":"Kuznetsov, Nikolai V.","last_name":"Kuznetsov"},{"first_name":"Evangelos","full_name":"Doukoumopoulos, Evangelos","last_name":"Doukoumopoulos"},{"first_name":"Liaqat","last_name":"Ali","full_name":"Ali, Liaqat"},{"full_name":"Oliveira, Mariana M. S.","last_name":"Oliveira","first_name":"Mariana M. S."},{"first_name":"Christina","full_name":"Seitz, Christina","last_name":"Seitz"},{"id":"45adb726-eb97-11eb-a6c2-c7c3d3caabe9","first_name":"Mathias","last_name":"Percipalle","full_name":"Percipalle, Mathias"},{"full_name":"Nikić, Tijana","last_name":"Nikić","first_name":"Tijana"},{"first_name":"Anastasia A.","full_name":"Sadova, Anastasia A.","last_name":"Sadova"},{"full_name":"Shulgina, Sofia M.","last_name":"Shulgina","first_name":"Sofia M."},{"last_name":"Shmarov","full_name":"Shmarov, Vjacheslav A.","first_name":"Vjacheslav A."},{"last_name":"Kutko","full_name":"Kutko, Olga V.","first_name":"Olga V."},{"first_name":"Daria D.","full_name":"Vlasova, Daria D.","last_name":"Vlasova"},{"first_name":"Kseniya D.","last_name":"Orlova","full_name":"Orlova, Kseniya D."},{"first_name":"Marina P.","full_name":"Rykova, Marina P.","last_name":"Rykova"},{"full_name":"Andersson, John","last_name":"Andersson","first_name":"John"},{"first_name":"Piergiorgio","last_name":"Percipalle","full_name":"Percipalle, Piergiorgio"},{"first_name":"Claudia","last_name":"Kutter","full_name":"Kutter, Claudia"},{"first_name":"Sergey A.","full_name":"Ponomarev, Sergey A.","last_name":"Ponomarev"},{"first_name":"Lisa S.","last_name":"Westerberg","full_name":"Westerberg, Lisa S."}],"abstract":[{"lang":"eng","text":"The next steps of deep space exploration are manned missions to Moon and Mars. For safe space missions for crew members, it is important to understand the impact of space flight on the immune system. We studied the effects of 21 days dry immersion (DI) exposure on the transcriptomes of T cells isolated from blood samples of eight healthy volunteers. Samples were collected 7 days before DI, at day 7, 14, and 21 during DI, and 7 days after DI. RNA sequencing of CD3+T cells revealed transcriptional alterations across all time points, with most changes occurring 14 days after DI exposure. At day 21, T cells showed evidence of adaptation with a transcriptional profile resembling that of 7 days before DI. At 7 days after DI, T cells again changed their transcriptional profile. These data suggest that T cells adapt by rewiring their transcriptomes in response to simulated weightlessness and that remodeling cues persist when reexposed to normal gravity."}],"citation":{"short":"C.J. Gallardo-Dodd, C. Oertlin, J. Record, R.G. Galvani, C. Sommerauer, N.V. Kuznetsov, E. Doukoumopoulos, L. Ali, M.M.S. Oliveira, C. Seitz, M. Percipalle, T. Nikić, A.A. Sadova, S.M. Shulgina, V.A. Shmarov, O.V. Kutko, D.D. Vlasova, K.D. Orlova, M.P. Rykova, J. Andersson, P. Percipalle, C. Kutter, S.A. Ponomarev, L.S. Westerberg, Science Advances 9 (2023).","ista":"Gallardo-Dodd CJ, Oertlin C, Record J, Galvani RG, Sommerauer C, Kuznetsov NV, Doukoumopoulos E, Ali L, Oliveira MMS, Seitz C, Percipalle M, Nikić T, Sadova AA, Shulgina SM, Shmarov VA, Kutko OV, Vlasova DD, Orlova KD, Rykova MP, Andersson J, Percipalle P, Kutter C, Ponomarev SA, Westerberg LS. 2023. Exposure of volunteers to microgravity by dry immersion bed over 21 days results in gene expression changes and adaptation of T cells. Science Advances. 9(34), adg1610.","ama":"Gallardo-Dodd CJ, Oertlin C, Record J, et al. Exposure of volunteers to microgravity by dry immersion bed over 21 days results in gene expression changes and adaptation of T cells. <i>Science Advances</i>. 2023;9(34). doi:<a href=\"https://doi.org/10.1126/sciadv.adg1610\">10.1126/sciadv.adg1610</a>","mla":"Gallardo-Dodd, Carlos J., et al. “Exposure of Volunteers to Microgravity by Dry Immersion Bed over 21 Days Results in Gene Expression Changes and Adaptation of T Cells.” <i>Science Advances</i>, vol. 9, no. 34, adg1610, American Association for the Advancement of Science, 2023, doi:<a href=\"https://doi.org/10.1126/sciadv.adg1610\">10.1126/sciadv.adg1610</a>.","chicago":"Gallardo-Dodd, Carlos J., Christian Oertlin, Julien Record, Rômulo G. Galvani, Christian Sommerauer, Nikolai V. Kuznetsov, Evangelos Doukoumopoulos, et al. “Exposure of Volunteers to Microgravity by Dry Immersion Bed over 21 Days Results in Gene Expression Changes and Adaptation of T Cells.” <i>Science Advances</i>. American Association for the Advancement of Science, 2023. <a href=\"https://doi.org/10.1126/sciadv.adg1610\">https://doi.org/10.1126/sciadv.adg1610</a>.","apa":"Gallardo-Dodd, C. J., Oertlin, C., Record, J., Galvani, R. G., Sommerauer, C., Kuznetsov, N. V., … Westerberg, L. S. (2023). Exposure of volunteers to microgravity by dry immersion bed over 21 days results in gene expression changes and adaptation of T cells. <i>Science Advances</i>. American Association for the Advancement of Science. <a href=\"https://doi.org/10.1126/sciadv.adg1610\">https://doi.org/10.1126/sciadv.adg1610</a>","ieee":"C. J. Gallardo-Dodd <i>et al.</i>, “Exposure of volunteers to microgravity by dry immersion bed over 21 days results in gene expression changes and adaptation of T cells,” <i>Science Advances</i>, vol. 9, no. 34. American Association for the Advancement of Science, 2023."},"publication_status":"published","oa_version":"Published Version","quality_controlled":"1","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","acknowledgement":"This work was supported by a postdoctoral fellowship from the Swedish Society for Medical Research to J.R., a CAPES-STINT joint grant to R.G.G. and L.S.W., a PhD fellowship from Karolinska Institutet (KID) to E.D., a PhD fellowship from Fundação para a Ciência e a Tecnologia and European Social Fund to M.M.S.O., the program of fundamental research (theme 65.1) of the Institute for Biomedical Problems of the Russian Academy of Sciences (IBMP RAS) to A.A.S., S.M.S., V.A.S., O.V.K., D.D.V., K.D.O., M.P.R., and S.A.P., the Tamkeen under the NYU Abu Dhabi Research Institute Award to the NYUAD Center for Genomics and Systems Biology (ADHPG-CGSB) to P.P., the Knut and Alice Wallenberg foundation to C.K., the Swedish National Space Agency to N.V.K. and L.S.W., Swedish Research Council, Gösta Fraenckel Foundation, and Karolinska Institutet to L.S.W.","publication_identifier":{"issn":["2375-2548"]},"_id":"14784","pmid":1,"article_processing_charge":"Yes","volume":9,"date_updated":"2024-01-16T09:38:58Z","oa":1,"title":"Exposure of volunteers to microgravity by dry immersion bed over 21 days results in gene expression changes and adaptation of T cells","external_id":{"isi":["001054596800007"],"pmid":["37624890"]},"year":"2023","doi":"10.1126/sciadv.adg1610","ddc":["570"],"isi":1,"article_number":"adg1610","tmp":{"image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"}},{"date_published":"2023-01-20T00:00:00Z","article_type":"original","month":"01","language":[{"iso":"eng"}],"scopus_import":"1","publisher":"American Association for the Advancement of Science","department":[{"_id":"FlSc"},{"_id":"EM-Fac"}],"has_accepted_license":"1","date_created":"2023-01-23T07:26:42Z","file":[{"success":1,"relation":"main_file","content_type":"application/pdf","creator":"dernst","file_id":"12335","file_size":1756234,"file_name":"2023_ScienceAdvances_Faessler.pdf","checksum":"ce81a6d0b84170e5e8c62f6acfa15d9e","date_created":"2023-01-23T07:45:54Z","access_level":"open_access","date_updated":"2023-01-23T07:45:54Z"}],"type":"journal_article","day":"20","status":"public","intvolume":"         9","file_date_updated":"2023-01-23T07:45:54Z","publication":"Science Advances","issue":"3","doi":"10.1126/sciadv.add6495","year":"2023","acknowledged_ssus":[{"_id":"ScienComp"},{"_id":"LifeSc"},{"_id":"Bio"},{"_id":"EM-Fac"}],"external_id":{"isi":["000964550100015"]},"title":"ArpC5 isoforms regulate Arp2/3 complex–dependent protrusion through differential Ena/VASP positioning","isi":1,"article_number":"add6495","tmp":{"image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"ddc":["570"],"related_material":{"record":[{"relation":"research_data","id":"14562","status":"public"}]},"citation":{"apa":"Fäßler, F., Javoor, M., Datler, J., Döring, H., Hofer, F., Dimchev, G. A., … Schur, F. K. (2023). ArpC5 isoforms regulate Arp2/3 complex–dependent protrusion through differential Ena/VASP positioning. <i>Science Advances</i>. American Association for the Advancement of Science. <a href=\"https://doi.org/10.1126/sciadv.add6495\">https://doi.org/10.1126/sciadv.add6495</a>","ieee":"F. Fäßler <i>et al.</i>, “ArpC5 isoforms regulate Arp2/3 complex–dependent protrusion through differential Ena/VASP positioning,” <i>Science Advances</i>, vol. 9, no. 3. American Association for the Advancement of Science, 2023.","chicago":"Fäßler, Florian, Manjunath Javoor, Julia Datler, Hermann Döring, Florian Hofer, Georgi A Dimchev, Victor-Valentin Hodirnau, Jan Faix, Klemens Rottner, and Florian KM Schur. “ArpC5 Isoforms Regulate Arp2/3 Complex–Dependent Protrusion through Differential Ena/VASP Positioning.” <i>Science Advances</i>. American Association for the Advancement of Science, 2023. <a href=\"https://doi.org/10.1126/sciadv.add6495\">https://doi.org/10.1126/sciadv.add6495</a>.","mla":"Fäßler, Florian, et al. “ArpC5 Isoforms Regulate Arp2/3 Complex–Dependent Protrusion through Differential Ena/VASP Positioning.” <i>Science Advances</i>, vol. 9, no. 3, add6495, American Association for the Advancement of Science, 2023, doi:<a href=\"https://doi.org/10.1126/sciadv.add6495\">10.1126/sciadv.add6495</a>.","ama":"Fäßler F, Javoor M, Datler J, et al. ArpC5 isoforms regulate Arp2/3 complex–dependent protrusion through differential Ena/VASP positioning. <i>Science Advances</i>. 2023;9(3). doi:<a href=\"https://doi.org/10.1126/sciadv.add6495\">10.1126/sciadv.add6495</a>","ista":"Fäßler F, Javoor M, Datler J, Döring H, Hofer F, Dimchev GA, Hodirnau V-V, Faix J, Rottner K, Schur FK. 2023. ArpC5 isoforms regulate Arp2/3 complex–dependent protrusion through differential Ena/VASP positioning. Science Advances. 9(3), add6495.","short":"F. Fäßler, M. Javoor, J. Datler, H. Döring, F. Hofer, G.A. Dimchev, V.-V. Hodirnau, J. Faix, K. Rottner, F.K. Schur, Science Advances 9 (2023)."},"publication_status":"published","keyword":["Multidisciplinary"],"author":[{"first_name":"Florian","orcid":"0000-0001-7149-769X","full_name":"Fäßler, Florian","last_name":"Fäßler","id":"404F5528-F248-11E8-B48F-1D18A9856A87"},{"id":"305ab18b-dc7d-11ea-9b2f-b58195228ea2","last_name":"Javoor","full_name":"Javoor, Manjunath","first_name":"Manjunath"},{"id":"3B12E2E6-F248-11E8-B48F-1D18A9856A87","last_name":"Datler","full_name":"Datler, Julia","orcid":"0000-0002-3616-8580","first_name":"Julia"},{"first_name":"Hermann","full_name":"Döring, Hermann","last_name":"Döring"},{"first_name":"Florian","last_name":"Hofer","full_name":"Hofer, Florian","id":"b9d234ba-9e33-11ed-95b6-cd561df280e6"},{"id":"38C393BE-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-8370-6161","last_name":"Dimchev","full_name":"Dimchev, Georgi A","first_name":"Georgi A"},{"id":"3661B498-F248-11E8-B48F-1D18A9856A87","last_name":"Hodirnau","full_name":"Hodirnau, Victor-Valentin","first_name":"Victor-Valentin"},{"first_name":"Jan","full_name":"Faix, Jan","last_name":"Faix"},{"full_name":"Rottner, Klemens","last_name":"Rottner","first_name":"Klemens"},{"id":"48AD8942-F248-11E8-B48F-1D18A9856A87","first_name":"Florian KM","full_name":"Schur, Florian KM","last_name":"Schur","orcid":"0000-0003-4790-8078"}],"abstract":[{"lang":"eng","text":"Regulation of the Arp2/3 complex is required for productive nucleation of branched actin networks. An emerging aspect of regulation is the incorporation of subunit isoforms into the Arp2/3 complex. Specifically, both ArpC5 subunit isoforms, ArpC5 and ArpC5L, have been reported to fine-tune nucleation activity and branch junction stability. We have combined reverse genetics and cellular structural biology to describe how ArpC5 and ArpC5L differentially affect cell migration. Both define the structural stability of ArpC1 in branch junctions and, in turn, by determining protrusion characteristics, affect protein dynamics and actin network ultrastructure. ArpC5 isoforms also affect the positioning of members of the Ena/Vasodilator-stimulated phosphoprotein (VASP) family of actin filament elongators, which mediate ArpC5 isoform–specific effects on the actin assembly level. Our results suggest that ArpC5 and Ena/VASP proteins are part of a signaling pathway enhancing cell migration.</jats:p>"}],"article_processing_charge":"No","oa":1,"volume":9,"date_updated":"2023-11-21T08:05:35Z","project":[{"grant_number":"P33367","name":"Structure and isoform diversity of the Arp2/3 complex","_id":"9B954C5C-BA93-11EA-9121-9846C619BF3A"}],"quality_controlled":"1","oa_version":"Published Version","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","acknowledgement":"We would like to thank K. von Peinen and B. Denker (Helmholtz Centre for Infection Research, Braunschweig, Germany) for experimental and technical assistance, respectively.\r\nThis research was supported by the Scientific Service Units (SSUs) of ISTA through resources provided by Scientific Computing (SciComp), the Life Science Facility (LSF), the Imaging and Optics facility (IOF), and the Electron Microscopy Facility (EMF). We acknowledge support from ISTA and from the Austrian Science Fund (FWF) (P33367) to F.K.M.S., from the Research Training Group GRK2223 and the Helmholtz Society to K.R,. and from the Deutsche Forschungsgemeinschaft (DFG) to J.F. and K.R.","publication_identifier":{"issn":["2375-2548"]},"_id":"12334"},{"type":"journal_article","day":"01","status":"public","intvolume":"         8","file_date_updated":"2023-03-21T14:18:10Z","issue":"44","publication":"Science Advances","date_published":"2022-11-01T00:00:00Z","article_type":"original","month":"11","language":[{"iso":"eng"}],"publisher":"American Association for the Advancement of Science","scopus_import":"1","department":[{"_id":"SiHi"}],"has_accepted_license":"1","file":[{"date_created":"2023-03-21T14:18:10Z","checksum":"0117023e188542082ca6693cf39e7f03","file_name":"sciadv.abq1263.pdf","file_size":2973998,"date_updated":"2023-03-21T14:18:10Z","access_level":"open_access","success":1,"creator":"patrickd","file_id":"12742","content_type":"application/pdf","relation":"main_file"}],"date_created":"2022-04-26T15:04:50Z","publication_status":"published","citation":{"chicago":"Amberg, Nicole, Florian Pauler, Carmen Streicher, and Simon Hippenmeyer. “Tissue-Wide Genetic and Cellular Landscape Shapes the Execution of Sequential PRC2 Functions in Neural Stem Cell Lineage Progression.” <i>Science Advances</i>. American Association for the Advancement of Science, 2022. <a href=\"https://doi.org/10.1126/sciadv.abq1263\">https://doi.org/10.1126/sciadv.abq1263</a>.","apa":"Amberg, N., Pauler, F., Streicher, C., &#38; Hippenmeyer, S. (2022). Tissue-wide genetic and cellular landscape shapes the execution of sequential PRC2 functions in neural stem cell lineage progression. <i>Science Advances</i>. American Association for the Advancement of Science. <a href=\"https://doi.org/10.1126/sciadv.abq1263\">https://doi.org/10.1126/sciadv.abq1263</a>","ieee":"N. Amberg, F. Pauler, C. Streicher, and S. Hippenmeyer, “Tissue-wide genetic and cellular landscape shapes the execution of sequential PRC2 functions in neural stem cell lineage progression,” <i>Science Advances</i>, vol. 8, no. 44. American Association for the Advancement of Science, 2022.","short":"N. Amberg, F. Pauler, C. Streicher, S. Hippenmeyer, Science Advances 8 (2022).","ista":"Amberg N, Pauler F, Streicher C, Hippenmeyer S. 2022. Tissue-wide genetic and cellular landscape shapes the execution of sequential PRC2 functions in neural stem cell lineage progression. Science Advances. 8(44), abq1263.","mla":"Amberg, Nicole, et al. “Tissue-Wide Genetic and Cellular Landscape Shapes the Execution of Sequential PRC2 Functions in Neural Stem Cell Lineage Progression.” <i>Science Advances</i>, vol. 8, no. 44, abq1263, American Association for the Advancement of Science, 2022, doi:<a href=\"https://doi.org/10.1126/sciadv.abq1263\">10.1126/sciadv.abq1263</a>.","ama":"Amberg N, Pauler F, Streicher C, Hippenmeyer S. Tissue-wide genetic and cellular landscape shapes the execution of sequential PRC2 functions in neural stem cell lineage progression. <i>Science Advances</i>. 2022;8(44). doi:<a href=\"https://doi.org/10.1126/sciadv.abq1263\">10.1126/sciadv.abq1263</a>"},"author":[{"id":"4CD6AAC6-F248-11E8-B48F-1D18A9856A87","first_name":"Nicole","orcid":"0000-0002-3183-8207","last_name":"Amberg","full_name":"Amberg, Nicole"},{"last_name":"Pauler","full_name":"Pauler, Florian","first_name":"Florian","id":"48EA0138-F248-11E8-B48F-1D18A9856A87"},{"id":"36BCB99C-F248-11E8-B48F-1D18A9856A87","full_name":"Streicher, Carmen","last_name":"Streicher","first_name":"Carmen"},{"id":"37B36620-F248-11E8-B48F-1D18A9856A87","full_name":"Hippenmeyer, Simon","last_name":"Hippenmeyer","orcid":"0000-0003-2279-1061","first_name":"Simon"}],"abstract":[{"text":"The generation of a correctly-sized cerebral cortex with all-embracing neuronal and glial cell-type diversity critically depends on faithful radial glial progenitor (RGP) cell proliferation/differentiation programs. Temporal RGP lineage progression is regulated by Polycomb Repressive Complex 2 (PRC2) and loss of PRC2 activity results in severe neurogenesis defects and microcephaly. How PRC2-dependent gene expression instructs RGP lineage progression is unknown. Here we utilize Mosaic Analysis with Double Markers (MADM)-based single cell technology and demonstrate that PRC2 is not cell-autonomously required in neurogenic RGPs but rather acts at the global tissue-wide level. Conversely, cortical astrocyte production and maturation is cell-autonomously controlled by PRC2-dependent transcriptional regulation. We thus reveal highly distinct and sequential PRC2 functions in RGP lineage progression that are dependent on complex interplays between intrinsic and tissue-wide properties. In a broader context our results imply a critical role for the genetic and cellular niche environment in neural stem cell behavior.","lang":"eng"}],"date_updated":"2023-05-31T12:24:10Z","oa":1,"volume":8,"article_processing_charge":"No","acknowledgement":"We thank A. Heger (IST Austria Preclinical Facility), A. Sommer and C. Czepe (VBCF GmbH, NGS  Unit)  and  S.  Gharagozlou  for  technical  support.  This  research  was  supported  by  the  Scientific  Service  Units  (SSU)  of  IST  Austria  through  resources  provided  by  the  Imaging  &  Optics Facility (IOF), Lab Support Facility (LSF), and Preclinical Facility (PCF). N.A. received funding   from   the   FWF   Firnberg-Programm   (T   1031).   The   work   was   supported   by   IST   institutional  funds  and  by  the  European  Research  Council  (ERC)  under  the  European  Union’s  Horizon 2020 research and innovation program (grant agreement 725780 LinPro) to S.H.","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","quality_controlled":"1","project":[{"grant_number":"725780","call_identifier":"H2020","name":"Principles of Neural Stem Cell Lineage Progression in Cerebral Cortex Development","_id":"260018B0-B435-11E9-9278-68D0E5697425"},{"grant_number":"T0101031","name":"Role of Eed in neural stem cell lineage progression","_id":"268F8446-B435-11E9-9278-68D0E5697425","call_identifier":"FWF"}],"oa_version":"Published Version","_id":"11336","publication_identifier":{"issn":["2375-2548"]},"ec_funded":1,"acknowledged_ssus":[{"_id":"PreCl"},{"_id":"Bio"},{"_id":"LifeSc"}],"doi":"10.1126/sciadv.abq1263","year":"2022","title":"Tissue-wide genetic and cellular landscape shapes the execution of sequential PRC2 functions in neural stem cell lineage progression","article_number":"abq1263","tmp":{"image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"ddc":["570"],"related_material":{"link":[{"relation":"press_release","description":"News on ISTA website","url":"https://ista.ac.at/en/news/whole-tissue-shapes-brain-development/"}]}},{"date_created":"2023-01-16T09:57:10Z","file":[{"date_updated":"2023-01-30T09:27:49Z","access_level":"open_access","file_name":"2022_ScienceAdvances_Stock.pdf","file_size":1636732,"date_created":"2023-01-30T09:27:49Z","checksum":"f59cdb824e5d4221045def81f46f6c65","content_type":"application/pdf","relation":"main_file","creator":"dernst","file_id":"12444","success":1}],"department":[{"_id":"EdHa"}],"has_accepted_license":"1","language":[{"iso":"eng"}],"publisher":"American Association for the Advancement of Science","scopus_import":"1","article_type":"original","date_published":"2022-09-14T00:00:00Z","month":"09","file_date_updated":"2023-01-30T09:27:49Z","issue":"37","publication":"Science Advances","status":"public","intvolume":"         8","type":"journal_article","day":"14","ddc":["570"],"article_number":"eadd2488","isi":1,"tmp":{"image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"external_id":{"isi":["000888875000009"],"pmid":["36103529"]},"title":"A self-generated Toddler gradient guides mesodermal cell migration","ec_funded":1,"year":"2022","doi":"10.1126/sciadv.add2488","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","acknowledgement":"We thank K. Aumayer and the team of the biooptics facility at the Vienna Biocenter, particularly P. Pasierbek and T. Müller, for support with microscopy; K. Panser, C. Pribitzer, and the animal facility personnel for taking care of zebrafish; M. Binner and A. Bandura for help with genotyping; M. Codina Tobias for help with establishing the conditions for the Toddler overexpression compensation experiment; T. Lubiana Alves for sharing the code for scRNA-Seq analyses; the Heisenberg laboratory, particularly D. Pinheiro, for joint laboratory meetings, discussions on the project, and providing the tg(gsc:CAAX-GFP) fish line; the Raz laboratory for providing the Lifeact-GFP plasmid; A. Andersen, A. Schier, C.-P. Heisenberg, and E. Tanaka for comments on the manuscript; and the entire Pauli laboratory, particularly K. Gert and V. Deneke, for valuable discussions and feedback on the manuscript. Funding: Work in A.P.’s laboratory has been supported by the IMP, which receives institutional funding from Boehringer Ingelheim and the Austrian Research Promotion Agency (Headquarter grant FFG-852936), as well as the FWF START program (Y 1031-B28 to A.P.), the Human Frontier Science Program (HFSP) Career Development Award (CDA00066/2015 to A.P.) and Young Investigator Grant (RGY0079/2020 to A.P.), the SFB RNA-Deco (project number F 80 to A.P.), a Whitman Center Fellowship from the Marine Biological Laboratory (to A.P.), and EMBO-YIP funds (to A.P.). This work was supported by the European Union (European Research Council Starting Grant 851288 to E.H.). For the purpose of Open Access, the authors have applied a CC BY public copyright license to any Author Accepted Manuscript (AAM) version arising from this submission.","oa_version":"Published Version","project":[{"grant_number":"851288","name":"Design Principles of Branching Morphogenesis","_id":"05943252-7A3F-11EA-A408-12923DDC885E","call_identifier":"H2020"}],"quality_controlled":"1","_id":"12253","pmid":1,"publication_identifier":{"issn":["2375-2548"]},"oa":1,"date_updated":"2023-08-04T09:49:59Z","volume":8,"article_processing_charge":"No","author":[{"first_name":"Jessica","full_name":"Stock, Jessica","last_name":"Stock"},{"first_name":"Tomas","last_name":"Kazmar","full_name":"Kazmar, Tomas"},{"last_name":"Schlumm","full_name":"Schlumm, Friederike","first_name":"Friederike"},{"id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","first_name":"Edouard B","full_name":"Hannezo, Edouard B","last_name":"Hannezo","orcid":"0000-0001-6005-1561"},{"last_name":"Pauli","full_name":"Pauli, Andrea","first_name":"Andrea"}],"abstract":[{"text":"The sculpting of germ layers during gastrulation relies on the coordinated migration of progenitor cells, yet the cues controlling these long-range directed movements remain largely unknown. While directional migration often relies on a chemokine gradient generated from a localized source, we find that zebrafish ventrolateral mesoderm is guided by a self-generated gradient of the initially uniformly expressed and secreted protein Toddler/ELABELA/Apela. We show that the Apelin receptor, which is specifically expressed in mesodermal cells, has a dual role during gastrulation, acting as a scavenger receptor to generate a Toddler gradient, and as a chemokine receptor to sense this guidance cue. Thus, we uncover a single receptor–based self-generated gradient as the enigmatic guidance cue that can robustly steer the directional migration of mesoderm through the complex and continuously changing environment of the gastrulating embryo.","lang":"eng"}],"publication_status":"published","citation":{"ieee":"J. Stock, T. Kazmar, F. Schlumm, E. B. Hannezo, and A. Pauli, “A self-generated Toddler gradient guides mesodermal cell migration,” <i>Science Advances</i>, vol. 8, no. 37. American Association for the Advancement of Science, 2022.","apa":"Stock, J., Kazmar, T., Schlumm, F., Hannezo, E. B., &#38; Pauli, A. (2022). A self-generated Toddler gradient guides mesodermal cell migration. <i>Science Advances</i>. American Association for the Advancement of Science. <a href=\"https://doi.org/10.1126/sciadv.add2488\">https://doi.org/10.1126/sciadv.add2488</a>","chicago":"Stock, Jessica, Tomas Kazmar, Friederike Schlumm, Edouard B Hannezo, and Andrea Pauli. “A Self-Generated Toddler Gradient Guides Mesodermal Cell Migration.” <i>Science Advances</i>. American Association for the Advancement of Science, 2022. <a href=\"https://doi.org/10.1126/sciadv.add2488\">https://doi.org/10.1126/sciadv.add2488</a>.","mla":"Stock, Jessica, et al. “A Self-Generated Toddler Gradient Guides Mesodermal Cell Migration.” <i>Science Advances</i>, vol. 8, no. 37, eadd2488, American Association for the Advancement of Science, 2022, doi:<a href=\"https://doi.org/10.1126/sciadv.add2488\">10.1126/sciadv.add2488</a>.","ama":"Stock J, Kazmar T, Schlumm F, Hannezo EB, Pauli A. A self-generated Toddler gradient guides mesodermal cell migration. <i>Science Advances</i>. 2022;8(37). doi:<a href=\"https://doi.org/10.1126/sciadv.add2488\">10.1126/sciadv.add2488</a>","ista":"Stock J, Kazmar T, Schlumm F, Hannezo EB, Pauli A. 2022. A self-generated Toddler gradient guides mesodermal cell migration. Science Advances. 8(37), eadd2488.","short":"J. Stock, T. Kazmar, F. Schlumm, E.B. Hannezo, A. Pauli, Science Advances 8 (2022)."}},{"ddc":["570"],"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by-nc/4.0/legalcode","image":"/images/cc_by_nc.png","name":"Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)","short":"CC BY-NC (4.0)"},"article_number":"eabd9153","isi":1,"title":"Optimal anchoring of a foldamer inhibitor of ASF1 histone chaperone through backbone plasticity","external_id":{"isi":["000633443000011"],"pmid":["33741589"]},"doi":"10.1126/sciadv.abd9153","year":"2021","pmid":1,"_id":"9262","publication_identifier":{"issn":["2375-2548"]},"user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","acknowledgement":"We thank the Synchrotron SOLEIL, the European Synchrotron Radiation Facility (ESRF), and the French Infrastructure for Integrated Structural Biology (FRISBI) ANR-10-INBS-05. We are particularly grateful to A. Clavier and A. Campalans for help in setting up and performing the cell penetration assays. Funding: Research was funded by the French Centre National de Recherche Scientifique (CNRS), the Commissariat à l’Energie Atomique (CEA), University of Bordeaux, University Paris-Saclay, and the Synchrotron Soleil. The project was supported by the ANR 2007 BREAKABOUND (JC-07-216078), 2011 BIPBIP (ANR-10-BINF-0003), 2012 CHAPINHIB (ANR-12-BSV5-0022-01), 2015 CHIPSET (ANR-15-CE11-008-01), 2015 HIMPP2I (ANR-15-CE07-0010), and the program labeled by the ARC foundation 2016 PGA1*20160203953). M.B. was supported by Canceropole (Paris, France) and a grant for young researchers from La Ligue contre le Cancer. J.M. was supported by La Ligue contre le Cancer.","quality_controlled":"1","oa_version":"Published Version","volume":7,"date_updated":"2023-08-07T14:20:26Z","oa":1,"article_processing_charge":"No","abstract":[{"lang":"eng","text":"Sequence-specific oligomers with predictable folding patterns, i.e., foldamers, provide new opportunities to mimic α-helical peptides and design inhibitors of protein-protein interactions. One major hurdle of this strategy is to retain the correct orientation of key side chains involved in protein surface recognition. Here, we show that the structural plasticity of a foldamer backbone may notably contribute to the required spatial adjustment for optimal interaction with the protein surface. By using oligoureas as α helix mimics, we designed a foldamer/peptide hybrid inhibitor of histone chaperone ASF1, a key regulator of chromatin dynamics. The crystal structure of its complex with ASF1 reveals a notable plasticity of the urea backbone, which adapts to the ASF1 surface to maintain the same binding interface. One additional benefit of generating ASF1 ligands with nonpeptide oligourea segments is the resistance to proteolysis in human plasma, which was highly improved compared to the cognate α-helical peptide."}],"author":[{"first_name":"Johanne","full_name":"Mbianda, Johanne","last_name":"Mbianda"},{"orcid":"0000-0002-9592-1587","full_name":"Bakail, May M","last_name":"Bakail","first_name":"May M","id":"FB3C3F8E-522F-11EA-B186-22963DDC885E"},{"full_name":"André, Christophe","last_name":"André","first_name":"Christophe"},{"last_name":"Moal","full_name":"Moal, Gwenaëlle","first_name":"Gwenaëlle"},{"first_name":"Marie E.","last_name":"Perrin","full_name":"Perrin, Marie E."},{"full_name":"Pinna, Guillaume","last_name":"Pinna","first_name":"Guillaume"},{"first_name":"Raphaël","full_name":"Guerois, Raphaël","last_name":"Guerois"},{"full_name":"Becher, Francois","last_name":"Becher","first_name":"Francois"},{"first_name":"Pierre","last_name":"Legrand","full_name":"Legrand, Pierre"},{"first_name":"Seydou","last_name":"Traoré","full_name":"Traoré, Seydou"},{"last_name":"Douat","full_name":"Douat, Céline","first_name":"Céline"},{"first_name":"Gilles","full_name":"Guichard, Gilles","last_name":"Guichard"},{"last_name":"Ochsenbein","full_name":"Ochsenbein, Françoise","first_name":"Françoise"}],"publication_status":"published","citation":{"short":"J. Mbianda, M.M. Bakail, C. André, G. Moal, M.E. Perrin, G. Pinna, R. Guerois, F. Becher, P. Legrand, S. Traoré, C. Douat, G. Guichard, F. Ochsenbein, Science Advances 7 (2021).","ista":"Mbianda J, Bakail MM, André C, Moal G, Perrin ME, Pinna G, Guerois R, Becher F, Legrand P, Traoré S, Douat C, Guichard G, Ochsenbein F. 2021. Optimal anchoring of a foldamer inhibitor of ASF1 histone chaperone through backbone plasticity. Science Advances. 7(12), eabd9153.","ama":"Mbianda J, Bakail MM, André C, et al. Optimal anchoring of a foldamer inhibitor of ASF1 histone chaperone through backbone plasticity. <i>Science Advances</i>. 2021;7(12). doi:<a href=\"https://doi.org/10.1126/sciadv.abd9153\">10.1126/sciadv.abd9153</a>","mla":"Mbianda, Johanne, et al. “Optimal Anchoring of a Foldamer Inhibitor of ASF1 Histone Chaperone through Backbone Plasticity.” <i>Science Advances</i>, vol. 7, no. 12, eabd9153, American Association for the Advancement of Science, 2021, doi:<a href=\"https://doi.org/10.1126/sciadv.abd9153\">10.1126/sciadv.abd9153</a>.","chicago":"Mbianda, Johanne, May M Bakail, Christophe André, Gwenaëlle Moal, Marie E. Perrin, Guillaume Pinna, Raphaël Guerois, et al. “Optimal Anchoring of a Foldamer Inhibitor of ASF1 Histone Chaperone through Backbone Plasticity.” <i>Science Advances</i>. American Association for the Advancement of Science, 2021. <a href=\"https://doi.org/10.1126/sciadv.abd9153\">https://doi.org/10.1126/sciadv.abd9153</a>.","apa":"Mbianda, J., Bakail, M. M., André, C., Moal, G., Perrin, M. E., Pinna, G., … Ochsenbein, F. (2021). Optimal anchoring of a foldamer inhibitor of ASF1 histone chaperone through backbone plasticity. <i>Science Advances</i>. American Association for the Advancement of Science. <a href=\"https://doi.org/10.1126/sciadv.abd9153\">https://doi.org/10.1126/sciadv.abd9153</a>","ieee":"J. Mbianda <i>et al.</i>, “Optimal anchoring of a foldamer inhibitor of ASF1 histone chaperone through backbone plasticity,” <i>Science Advances</i>, vol. 7, no. 12. American Association for the Advancement of Science, 2021."},"file":[{"success":1,"file_id":"9280","creator":"dernst","content_type":"application/pdf","relation":"main_file","date_created":"2021-03-22T12:49:00Z","checksum":"737624cd0e630ffa7c52797a690500e3","file_name":"2021_ScienceAdv_Mbianda.pdf","file_size":837156,"date_updated":"2021-03-22T12:49:00Z","access_level":"open_access"}],"date_created":"2021-03-22T07:14:03Z","has_accepted_license":"1","department":[{"_id":"CampIT"}],"publisher":"American Association for the Advancement of Science","language":[{"iso":"eng"}],"month":"03","article_type":"original","date_published":"2021-03-19T00:00:00Z","issue":"12","publication":"Science Advances","file_date_updated":"2021-03-22T12:49:00Z","intvolume":"         7","status":"public","day":"19","type":"journal_article"},{"has_accepted_license":"1","date_created":"2021-11-26T06:40:28Z","file":[{"checksum":"3ba2eca975930cdb0b1ce1ae876885a7","date_created":"2021-11-26T06:50:09Z","file_size":10381298,"file_name":"2020_SciAdv_Tian.pdf","access_level":"open_access","date_updated":"2021-11-26T06:50:09Z","success":1,"file_id":"10343","creator":"cchlebak","relation":"main_file","content_type":"application/pdf"}],"date_published":"2020-11-27T00:00:00Z","article_type":"original","month":"11","language":[{"iso":"eng"}],"publisher":"American Association for the Advancement of Science","scopus_import":"1","file_date_updated":"2021-11-26T06:50:09Z","issue":"48","publication":"Science Advances","type":"journal_article","day":"27","status":"public","intvolume":"         6","article_number":"eabc4397 ","main_file_link":[{"url":"https://www.biorxiv.org/content/10.1101/2020.04.04.025866v1","open_access":"1"}],"tmp":{"image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"ddc":["611"],"doi":"10.1126/sciadv.abc4397","year":"2020","external_id":{"pmid":["33246953"]},"title":"On the shuttling across the blood-brain barrier via tubule formation: Mechanism and cargo avidity bias","date_updated":"2021-11-26T07:00:24Z","oa":1,"volume":6,"article_processing_charge":"No","acknowledgement":"Funding: G.B. thanks the ERC for the starting grant (MEViC 278793) and consolidator award (CheSSTaG 769798), EPSRC/BTG Healthcare Partnership (EP/I001697/1), EPSRC Established Career Fellowship (EP/N026322/1), EPSRC/SomaNautix Healthcare Partnership EP/R024723/1, and Children with Cancer UK for the research project (16-227). X.T. and G.B. thank that Anhui 100 Talent program for facilitating data sharing and research visits. A.D.-C. and L.R. acknowledge the Royal Society for a Newton fellowship and the Marie Skłodowska-Curie Actions for a European Fellowship. Author contributions: X.T. prepared and characterized POs, performed all the fast imaging in both conventional and STED microscopy, set up the initial BBB model, encapsulated the PtA2 in POs, and supervised the PtA2-PO animal work. D.M.L. prepared and characterized POs; performed all the permeability studies, PLA assays, WB and associated data analysis, and part of the colocalization assays; and performed experiments with the shRNA for knockdown of syndapin-2. E.S. prepared and characterized POs and performed part of colocalization assays and Cy7-labeled PO animal experiments. S.N. prepared and characterized POs and performed part of the colocalization and inhibition assays. G.F. designed, performed, and analyzed the agent-based simulations of transcytosis. J.F. designed the image-based algorithm to analyze the PLA data. D.M. prepared and characterized POs and helped with Cy7-labeled PO animal experiments. A.A. performed TEM imaging of the POs. A.P. and A.D.-C. synthesized the dye- and peptide-functionalized and pristine copolymers. M.V., L.H.-K., and A.Š. designed, performed, and analyzed the MD simulations. Z.Z. supervised and supported STED imaging. P.X., B.F., and Y.T. synthesized and characterized the PtA2 compound. L.L. performed some of the animal work. L.R. supported and helped with the BBB characterization. G.B. analyzed all fast imaging and supervised and coordinated the overall work. X.T., D.M.L., E.S., and G.B. wrote the manuscript. Competing interests: The authors declare that part of the work is associated with the UCL spin-out company SomaNautix Ltd. Data and materials availability: All data needed to evaluate the conclusions in the paper are present in the paper and/or the Supplementary Materials. Additional data related to this paper may be requested from the authors.","user_id":"8b945eb4-e2f2-11eb-945a-df72226e66a9","oa_version":"Published Version","quality_controlled":"1","pmid":1,"_id":"10342","publication_identifier":{"issn":["2375-2548"]},"extern":"1","publication_status":"published","citation":{"short":"X. Tian, D.M. Leite, E. Scarpa, S. Nyberg, G. Fullstone, J. Forth, D. Matias, A. Apriceno, A. Poma, A. Duro-Castano, M. Vuyyuru, L. Harker-Kirschneck, A. Šarić, Z. Zhang, P. Xiang, B. Fang, Y. Tian, L. Luo, L. Rizzello, G. Battaglia, Science Advances 6 (2020).","ista":"Tian X, Leite DM, Scarpa E, Nyberg S, Fullstone G, Forth J, Matias D, Apriceno A, Poma A, Duro-Castano A, Vuyyuru M, Harker-Kirschneck L, Šarić A, Zhang Z, Xiang P, Fang B, Tian Y, Luo L, Rizzello L, Battaglia G. 2020. On the shuttling across the blood-brain barrier via tubule formation: Mechanism and cargo avidity bias. Science Advances. 6(48), eabc4397.","ama":"Tian X, Leite DM, Scarpa E, et al. On the shuttling across the blood-brain barrier via tubule formation: Mechanism and cargo avidity bias. <i>Science Advances</i>. 2020;6(48). doi:<a href=\"https://doi.org/10.1126/sciadv.abc4397\">10.1126/sciadv.abc4397</a>","mla":"Tian, Xiaohe, et al. “On the Shuttling across the Blood-Brain Barrier via Tubule Formation: Mechanism and Cargo Avidity Bias.” <i>Science Advances</i>, vol. 6, no. 48, eabc4397, American Association for the Advancement of Science, 2020, doi:<a href=\"https://doi.org/10.1126/sciadv.abc4397\">10.1126/sciadv.abc4397</a>.","chicago":"Tian, Xiaohe, Diana M. Leite, Edoardo Scarpa, Sophie Nyberg, Gavin Fullstone, Joe Forth, Diana Matias, et al. “On the Shuttling across the Blood-Brain Barrier via Tubule Formation: Mechanism and Cargo Avidity Bias.” <i>Science Advances</i>. American Association for the Advancement of Science, 2020. <a href=\"https://doi.org/10.1126/sciadv.abc4397\">https://doi.org/10.1126/sciadv.abc4397</a>.","apa":"Tian, X., Leite, D. M., Scarpa, E., Nyberg, S., Fullstone, G., Forth, J., … Battaglia, G. (2020). On the shuttling across the blood-brain barrier via tubule formation: Mechanism and cargo avidity bias. <i>Science Advances</i>. American Association for the Advancement of Science. <a href=\"https://doi.org/10.1126/sciadv.abc4397\">https://doi.org/10.1126/sciadv.abc4397</a>","ieee":"X. Tian <i>et al.</i>, “On the shuttling across the blood-brain barrier via tubule formation: Mechanism and cargo avidity bias,” <i>Science Advances</i>, vol. 6, no. 48. American Association for the Advancement of Science, 2020."},"author":[{"first_name":"Xiaohe","last_name":"Tian","full_name":"Tian, Xiaohe"},{"last_name":"Leite","full_name":"Leite, Diana M.","first_name":"Diana M."},{"last_name":"Scarpa","full_name":"Scarpa, Edoardo","first_name":"Edoardo"},{"full_name":"Nyberg, Sophie","last_name":"Nyberg","first_name":"Sophie"},{"full_name":"Fullstone, Gavin","last_name":"Fullstone","first_name":"Gavin"},{"first_name":"Joe","last_name":"Forth","full_name":"Forth, Joe"},{"first_name":"Diana","last_name":"Matias","full_name":"Matias, Diana"},{"last_name":"Apriceno","full_name":"Apriceno, Azzurra","first_name":"Azzurra"},{"first_name":"Alessandro","full_name":"Poma, Alessandro","last_name":"Poma"},{"first_name":"Aroa","full_name":"Duro-Castano, Aroa","last_name":"Duro-Castano"},{"first_name":"Manish","last_name":"Vuyyuru","full_name":"Vuyyuru, Manish"},{"full_name":"Harker-Kirschneck, Lena","last_name":"Harker-Kirschneck","first_name":"Lena"},{"id":"bf63d406-f056-11eb-b41d-f263a6566d8b","first_name":"Anđela","last_name":"Šarić","full_name":"Šarić, Anđela","orcid":"0000-0002-7854-2139"},{"first_name":"Zhongping","last_name":"Zhang","full_name":"Zhang, Zhongping"},{"last_name":"Xiang","full_name":"Xiang, Pan","first_name":"Pan"},{"last_name":"Fang","full_name":"Fang, Bin","first_name":"Bin"},{"full_name":"Tian, Yupeng","last_name":"Tian","first_name":"Yupeng"},{"first_name":"Lei","full_name":"Luo, Lei","last_name":"Luo"},{"first_name":"Loris","last_name":"Rizzello","full_name":"Rizzello, Loris"},{"first_name":"Giuseppe","last_name":"Battaglia","full_name":"Battaglia, Giuseppe"}],"keyword":["multidisciplinary"],"abstract":[{"text":"The blood-brain barrier is made of polarized brain endothelial cells (BECs) phenotypically conditioned by the central nervous system (CNS). Although transport across BECs is of paramount importance for nutrient uptake as well as ridding the brain of waste products, the intracellular sorting mechanisms that regulate successful receptor-mediated transcytosis in BECs remain to be elucidated. Here, we used a synthetic multivalent system with tunable avidity to the low-density lipoprotein receptor–related protein 1 (LRP1) to investigate the mechanisms of transport across BECs. We used a combination of conventional and super-resolution microscopy, both in vivo and in vitro, accompanied with biophysical modeling of transport kinetics and membrane-bound interactions to elucidate the role of membrane-sculpting protein syndapin-2 on fast transport via tubule formation. We show that high-avidity cargo biases the LRP1 toward internalization associated with fast degradation, while mid-avidity augments the formation of syndapin-2 tubular carriers promoting a fast shuttling across.","lang":"eng"}]},{"citation":{"ieee":"J. Felix <i>et al.</i>, “Mechanism of the allosteric activation of the ClpP protease machinery by substrates and active-site inhibitors,” <i>Science Advances</i>, vol. 5, no. 9. American Association for the Advancement of Science, 2019.","apa":"Felix, J., Weinhäupl, K., Chipot, C., Dehez, F., Hessel, A., Gauto, D. F., … Fraga, H. (2019). Mechanism of the allosteric activation of the ClpP protease machinery by substrates and active-site inhibitors. <i>Science Advances</i>. American Association for the Advancement of Science. <a href=\"https://doi.org/10.1126/sciadv.aaw3818\">https://doi.org/10.1126/sciadv.aaw3818</a>","chicago":"Felix, Jan, Katharina Weinhäupl, Christophe Chipot, François Dehez, Audrey Hessel, Diego F. Gauto, Cecile Morlot, et al. “Mechanism of the Allosteric Activation of the ClpP Protease Machinery by Substrates and Active-Site Inhibitors.” <i>Science Advances</i>. American Association for the Advancement of Science, 2019. <a href=\"https://doi.org/10.1126/sciadv.aaw3818\">https://doi.org/10.1126/sciadv.aaw3818</a>.","ama":"Felix J, Weinhäupl K, Chipot C, et al. Mechanism of the allosteric activation of the ClpP protease machinery by substrates and active-site inhibitors. <i>Science Advances</i>. 2019;5(9). doi:<a href=\"https://doi.org/10.1126/sciadv.aaw3818\">10.1126/sciadv.aaw3818</a>","mla":"Felix, Jan, et al. “Mechanism of the Allosteric Activation of the ClpP Protease Machinery by Substrates and Active-Site Inhibitors.” <i>Science Advances</i>, vol. 5, no. 9, eaaw3818, American Association for the Advancement of Science, 2019, doi:<a href=\"https://doi.org/10.1126/sciadv.aaw3818\">10.1126/sciadv.aaw3818</a>.","ista":"Felix J, Weinhäupl K, Chipot C, Dehez F, Hessel A, Gauto DF, Morlot C, Abian O, Gutsche I, Velazquez-Campoy A, Schanda P, Fraga H. 2019. Mechanism of the allosteric activation of the ClpP protease machinery by substrates and active-site inhibitors. Science Advances. 5(9), eaaw3818.","short":"J. Felix, K. Weinhäupl, C. Chipot, F. Dehez, A. Hessel, D.F. Gauto, C. Morlot, O. Abian, I. Gutsche, A. Velazquez-Campoy, P. Schanda, H. Fraga, Science Advances 5 (2019)."},"publication_status":"published","author":[{"full_name":"Felix, Jan","last_name":"Felix","first_name":"Jan"},{"first_name":"Katharina","full_name":"Weinhäupl, Katharina","last_name":"Weinhäupl"},{"full_name":"Chipot, Christophe","last_name":"Chipot","first_name":"Christophe"},{"last_name":"Dehez","full_name":"Dehez, François","first_name":"François"},{"full_name":"Hessel, Audrey","last_name":"Hessel","first_name":"Audrey"},{"last_name":"Gauto","full_name":"Gauto, Diego F.","first_name":"Diego F."},{"first_name":"Cecile","last_name":"Morlot","full_name":"Morlot, Cecile"},{"first_name":"Olga","full_name":"Abian, Olga","last_name":"Abian"},{"first_name":"Irina","last_name":"Gutsche","full_name":"Gutsche, Irina"},{"first_name":"Adrian","full_name":"Velazquez-Campoy, Adrian","last_name":"Velazquez-Campoy"},{"id":"7B541462-FAF6-11E9-A490-E8DFE5697425","first_name":"Paul","orcid":"0000-0002-9350-7606","last_name":"Schanda","full_name":"Schanda, Paul"},{"first_name":"Hugo","last_name":"Fraga","full_name":"Fraga, Hugo"}],"abstract":[{"text":"Coordinated conformational transitions in oligomeric enzymatic complexes modulate function in response to substrates and play a crucial role in enzyme inhibition and activation. Caseinolytic protease (ClpP) is a tetradecameric complex, which has emerged as a drug target against multiple pathogenic bacteria. Activation of different ClpPs by inhibitors has been independently reported from drug development efforts, but no rationale for inhibitor-induced activation has been hitherto proposed. Using an integrated approach that includes x-ray crystallography, solid- and solution-state nuclear magnetic resonance, molecular dynamics simulations, and isothermal titration calorimetry, we show that the proteasome inhibitor bortezomib binds to the ClpP active-site serine, mimicking a peptide substrate, and induces a concerted allosteric activation of the complex. The bortezomib-activated conformation also exhibits a higher affinity for its cognate unfoldase ClpX. We propose a universal allosteric mechanism, where substrate binding to a single subunit locks ClpP into an active conformation optimized for chaperone association and protein processive degradation.","lang":"eng"}],"article_processing_charge":"No","volume":5,"date_updated":"2021-01-12T08:19:03Z","oa":1,"quality_controlled":"1","oa_version":"Published Version","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","publication_identifier":{"issn":["2375-2548"]},"extern":"1","_id":"8406","year":"2019","doi":"10.1126/sciadv.aaw3818","title":"Mechanism of the allosteric activation of the ClpP protease machinery by substrates and active-site inhibitors","main_file_link":[{"open_access":"1","url":" https://doi.org/10.1126/sciadv.aaw3818"}],"article_number":"eaaw3818","type":"journal_article","day":"04","status":"public","intvolume":"         5","publication":"Science Advances","issue":"9","article_type":"original","date_published":"2019-09-04T00:00:00Z","month":"09","language":[{"iso":"eng"}],"publisher":"American Association for the Advancement of Science","date_created":"2020-09-17T10:28:36Z"},{"abstract":[{"text":"The study of parallel ecological divergence provides important clues to the operation of natural selection. Parallel divergence often occurs in heterogeneous environments with different kinds of environmental gradients in different locations, but the genomic basis underlying this process is unknown. We investigated the genomics of rapid parallel adaptation in the marine snail Littorina saxatilis in response to two independent environmental axes (crab-predation versus wave-action and low-shore versus high-shore). Using pooled whole-genome resequencing, we show that sharing of genomic regions of high differentiation between environments is generally low but increases at smaller spatial scales. We identify different shared genomic regions of divergence for each environmental axis and show that most of these regions overlap with candidate chromosomal inversions. Several inversion regions are divergent and polymorphic across many localities. We argue that chromosomal inversions could store shared variation that fuels rapid parallel adaptation to heterogeneous environments, possibly as balanced polymorphism shared by adaptive gene flow.","lang":"eng"}],"author":[{"first_name":"Hernán E.","full_name":"Morales, Hernán E.","last_name":"Morales"},{"full_name":"Faria, Rui","last_name":"Faria","first_name":"Rui"},{"first_name":"Kerstin","full_name":"Johannesson, Kerstin","last_name":"Johannesson"},{"first_name":"Tomas","full_name":"Larsson, Tomas","last_name":"Larsson"},{"first_name":"Marina","full_name":"Panova, Marina","last_name":"Panova"},{"first_name":"Anja M","orcid":"0000-0003-1050-4969","full_name":"Westram, Anja M","last_name":"Westram","id":"3C147470-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Butlin","full_name":"Butlin, Roger K.","first_name":"Roger K."}],"citation":{"chicago":"Morales, Hernán E., Rui Faria, Kerstin Johannesson, Tomas Larsson, Marina Panova, Anja M Westram, and Roger K. Butlin. “Genomic Architecture of Parallel Ecological Divergence: Beyond a Single Environmental Contrast.” <i>Science Advances</i>. AAAS, 2019. <a href=\"https://doi.org/10.1126/sciadv.aav9963\">https://doi.org/10.1126/sciadv.aav9963</a>.","ieee":"H. E. Morales <i>et al.</i>, “Genomic architecture of parallel ecological divergence: Beyond a single environmental contrast,” <i>Science Advances</i>, vol. 5, no. 12. AAAS, 2019.","apa":"Morales, H. E., Faria, R., Johannesson, K., Larsson, T., Panova, M., Westram, A. M., &#38; Butlin, R. K. (2019). Genomic architecture of parallel ecological divergence: Beyond a single environmental contrast. <i>Science Advances</i>. AAAS. <a href=\"https://doi.org/10.1126/sciadv.aav9963\">https://doi.org/10.1126/sciadv.aav9963</a>","ista":"Morales HE, Faria R, Johannesson K, Larsson T, Panova M, Westram AM, Butlin RK. 2019. Genomic architecture of parallel ecological divergence: Beyond a single environmental contrast. Science Advances. 5(12), eaav9963.","short":"H.E. Morales, R. Faria, K. Johannesson, T. Larsson, M. Panova, A.M. Westram, R.K. Butlin, Science Advances 5 (2019).","mla":"Morales, Hernán E., et al. “Genomic Architecture of Parallel Ecological Divergence: Beyond a Single Environmental Contrast.” <i>Science Advances</i>, vol. 5, no. 12, eaav9963, AAAS, 2019, doi:<a href=\"https://doi.org/10.1126/sciadv.aav9963\">10.1126/sciadv.aav9963</a>.","ama":"Morales HE, Faria R, Johannesson K, et al. Genomic architecture of parallel ecological divergence: Beyond a single environmental contrast. <i>Science Advances</i>. 2019;5(12). doi:<a href=\"https://doi.org/10.1126/sciadv.aav9963\">10.1126/sciadv.aav9963</a>"},"publication_status":"published","publication_identifier":{"issn":["2375-2548"]},"pmid":1,"_id":"7393","project":[{"_id":"260C2330-B435-11E9-9278-68D0E5697425","name":"ISTplus - Postdoctoral Fellowships","call_identifier":"H2020","grant_number":"754411"},{"call_identifier":"H2020","name":"Theoretical and empirical approaches to understanding Parallel Adaptation","_id":"265B41B8-B435-11E9-9278-68D0E5697425","grant_number":"797747"}],"oa_version":"Published Version","quality_controlled":"1","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","article_processing_charge":"No","volume":5,"oa":1,"date_updated":"2023-09-06T15:35:56Z","external_id":{"pmid":["31840052"],"isi":["000505069600008"]},"title":"Genomic architecture of parallel ecological divergence: Beyond a single environmental contrast","doi":"10.1126/sciadv.aav9963","year":"2019","ec_funded":1,"ddc":["570"],"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by-nc/4.0/legalcode","image":"/images/cc_by_nc.png","name":"Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)","short":"CC BY-NC (4.0)"},"isi":1,"article_number":"eaav9963","intvolume":"         5","status":"public","day":"04","type":"journal_article","publication":"Science Advances","issue":"12","file_date_updated":"2020-07-14T12:47:57Z","scopus_import":"1","publisher":"AAAS","language":[{"iso":"eng"}],"month":"12","date_published":"2019-12-04T00:00:00Z","article_type":"original","file":[{"file_id":"7442","creator":"dernst","relation":"main_file","content_type":"application/pdf","checksum":"af99a5dcdc66c6d6102051faf3be48d8","date_created":"2020-02-03T13:33:25Z","file_size":1869449,"file_name":"2019_ScienceAdvances_Morales.pdf","access_level":"open_access","date_updated":"2020-07-14T12:47:57Z"}],"date_created":"2020-01-29T15:58:27Z","has_accepted_license":"1","department":[{"_id":"NiBa"}]},{"date_published":"2018-09-19T00:00:00Z","article_type":"original","doi":"10.1126/sciadv.aau4196","year":"2018","month":"09","language":[{"iso":"eng"}],"title":"Structural investigation of a chaperonin in action reveals how nucleotide binding regulates the functional cycle","publisher":"American Association for the Advancement of Science","article_number":"eaau4196","date_created":"2020-09-18T10:04:51Z","type":"journal_article","day":"19","citation":{"short":"G. Mas, J.-Y. Guan, E. Crublet, E.C. Debled, C. Moriscot, P. Gans, G. Schoehn, P. Macek, P. Schanda, J. Boisbouvier, Science Advances 4 (2018).","ista":"Mas G, Guan J-Y, Crublet E, Debled EC, Moriscot C, Gans P, Schoehn G, Macek P, Schanda P, Boisbouvier J. 2018. Structural investigation of a chaperonin in action reveals how nucleotide binding regulates the functional cycle. Science Advances. 4(9), eaau4196.","mla":"Mas, Guillaume, et al. “Structural Investigation of a Chaperonin in Action Reveals How Nucleotide Binding Regulates the Functional Cycle.” <i>Science Advances</i>, vol. 4, no. 9, eaau4196, American Association for the Advancement of Science, 2018, doi:<a href=\"https://doi.org/10.1126/sciadv.aau4196\">10.1126/sciadv.aau4196</a>.","ama":"Mas G, Guan J-Y, Crublet E, et al. Structural investigation of a chaperonin in action reveals how nucleotide binding regulates the functional cycle. <i>Science Advances</i>. 2018;4(9). doi:<a href=\"https://doi.org/10.1126/sciadv.aau4196\">10.1126/sciadv.aau4196</a>","chicago":"Mas, Guillaume, Jia-Ying Guan, Elodie Crublet, Elisa Colas Debled, Christine Moriscot, Pierre Gans, Guy Schoehn, Pavel Macek, Paul Schanda, and Jerome Boisbouvier. “Structural Investigation of a Chaperonin in Action Reveals How Nucleotide Binding Regulates the Functional Cycle.” <i>Science Advances</i>. American Association for the Advancement of Science, 2018. <a href=\"https://doi.org/10.1126/sciadv.aau4196\">https://doi.org/10.1126/sciadv.aau4196</a>.","ieee":"G. Mas <i>et al.</i>, “Structural investigation of a chaperonin in action reveals how nucleotide binding regulates the functional cycle,” <i>Science Advances</i>, vol. 4, no. 9. American Association for the Advancement of Science, 2018.","apa":"Mas, G., Guan, J.-Y., Crublet, E., Debled, E. C., Moriscot, C., Gans, P., … Boisbouvier, J. (2018). Structural investigation of a chaperonin in action reveals how nucleotide binding regulates the functional cycle. <i>Science Advances</i>. American Association for the Advancement of Science. <a href=\"https://doi.org/10.1126/sciadv.aau4196\">https://doi.org/10.1126/sciadv.aau4196</a>"},"publication_status":"published","author":[{"first_name":"Guillaume","last_name":"Mas","full_name":"Mas, Guillaume"},{"last_name":"Guan","full_name":"Guan, Jia-Ying","first_name":"Jia-Ying"},{"full_name":"Crublet, Elodie","last_name":"Crublet","first_name":"Elodie"},{"first_name":"Elisa Colas","last_name":"Debled","full_name":"Debled, Elisa Colas"},{"full_name":"Moriscot, Christine","last_name":"Moriscot","first_name":"Christine"},{"last_name":"Gans","full_name":"Gans, Pierre","first_name":"Pierre"},{"first_name":"Guy","last_name":"Schoehn","full_name":"Schoehn, Guy"},{"first_name":"Pavel","full_name":"Macek, Pavel","last_name":"Macek"},{"last_name":"Schanda","full_name":"Schanda, Paul","orcid":"0000-0002-9350-7606","first_name":"Paul","id":"7B541462-FAF6-11E9-A490-E8DFE5697425"},{"first_name":"Jerome","last_name":"Boisbouvier","full_name":"Boisbouvier, Jerome"}],"status":"public","intvolume":"         4","abstract":[{"lang":"eng","text":"Chaperonins are ubiquitous protein assemblies present in bacteria, eukaryota, and archaea, facilitating the folding of proteins, preventing protein aggregation, and thus participating in maintaining protein homeostasis in the cell. During their functional cycle, they bind unfolded client proteins inside their double ring structure and promote protein folding by closing the ring chamber in an adenosine 5′-triphosphate (ATP)–dependent manner. Although the static structures of fully open and closed forms of chaperonins were solved by x-ray crystallography or electron microscopy, elucidating the mechanisms of such ATP-driven molecular events requires studying the proteins at the structural level under working conditions. We introduce an approach that combines site-specific nuclear magnetic resonance observation of very large proteins, enabled by advanced isotope labeling methods, with an in situ ATP regeneration system. Using this method, we provide functional insight into the 1-MDa large hsp60 chaperonin while processing client proteins and reveal how nucleotide binding, hydrolysis, and release control switching between closed and open states. While the open conformation stabilizes the unfolded state of client proteins, the internalization of the client protein inside the chaperonin cavity speeds up its functional cycle. This approach opens new perspectives to study structures and mechanisms of various ATP-driven biological machineries in the heat of action."}],"article_processing_charge":"No","volume":4,"date_updated":"2022-08-26T09:11:06Z","publication":"Science Advances","issue":"9","quality_controlled":"1","oa_version":"None","user_id":"3E5EF7F0-F248-11E8-B48F-1D18A9856A87","publication_identifier":{"issn":["2375-2548"]},"extern":"1","_id":"8437"},{"language":[{"iso":"eng"}],"publisher":"American Association for the Advancement of Science ","scopus_import":"1","date_published":"2015-05-01T00:00:00Z","article_type":"original","month":"05","file":[{"relation":"main_file","content_type":"application/pdf","creator":"cziletti","file_id":"9058","success":1,"access_level":"open_access","date_updated":"2021-02-02T13:22:19Z","file_size":2416780,"file_name":"2015_ScienceAdvances_Palacci.pdf","checksum":"b97d62433581875c1b85210c5f6ae370","date_created":"2021-02-02T13:22:19Z"}],"date_created":"2021-02-02T13:15:02Z","has_accepted_license":"1","status":"public","intvolume":"         1","type":"journal_article","day":"01","file_date_updated":"2021-02-02T13:22:19Z","issue":"4","publication":"Science Advances","external_id":{"pmid":["26601175"],"arxiv":["1505.05111"]},"title":"Artificial rheotaxis","doi":"10.1126/sciadv.1400214","year":"2015","ddc":["530"],"article_number":"e1400214","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by-nc/4.0/legalcode","image":"/images/cc_by_nc.png","name":"Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)","short":"CC BY-NC (4.0)"},"author":[{"first_name":"Jérémie A","orcid":"0000-0002-7253-9465","full_name":"Palacci, Jérémie A","last_name":"Palacci","id":"8fb92548-2b22-11eb-b7c1-a3f0d08d7c7d"},{"full_name":"Sacanna, Stefano","last_name":"Sacanna","first_name":"Stefano"},{"first_name":"Anaïs","last_name":"Abramian","full_name":"Abramian, Anaïs"},{"full_name":"Barral, Jérémie","last_name":"Barral","first_name":"Jérémie"},{"first_name":"Kasey","last_name":"Hanson","full_name":"Hanson, Kasey"},{"first_name":"Alexander Y.","last_name":"Grosberg","full_name":"Grosberg, Alexander Y."},{"last_name":"Pine","full_name":"Pine, David J.","first_name":"David J."},{"first_name":"Paul M.","full_name":"Chaikin, Paul M.","last_name":"Chaikin"}],"abstract":[{"text":"Motility is a basic feature of living microorganisms, and how it works is often determined by environmental cues. Recent efforts have focused on developing artificial systems that can mimic microorganisms, in particular their self-propulsion. We report on the design and characterization of synthetic self-propelled particles that migrate upstream, known as positive rheotaxis. This phenomenon results from a purely physical mechanism involving the interplay between the polarity of the particles and their alignment by a viscous torque. We show quantitative agreement between experimental data and a simple model of an overdamped Brownian pendulum. The model notably predicts the existence of a stagnation point in a diverging flow. We take advantage of this property to demonstrate that our active particles can sense and predictably organize in an imposed flow. Our colloidal system represents an important step toward the realization of biomimetic microsystems with the ability to sense and respond to environmental changes.","lang":"eng"}],"publication_status":"published","citation":{"mla":"Palacci, Jérémie A., et al. “Artificial Rheotaxis.” <i>Science Advances</i>, vol. 1, no. 4, e1400214, American Association for the Advancement of Science , 2015, doi:<a href=\"https://doi.org/10.1126/sciadv.1400214\">10.1126/sciadv.1400214</a>.","ama":"Palacci JA, Sacanna S, Abramian A, et al. Artificial rheotaxis. <i>Science Advances</i>. 2015;1(4). doi:<a href=\"https://doi.org/10.1126/sciadv.1400214\">10.1126/sciadv.1400214</a>","ista":"Palacci JA, Sacanna S, Abramian A, Barral J, Hanson K, Grosberg AY, Pine DJ, Chaikin PM. 2015. Artificial rheotaxis. Science Advances. 1(4), e1400214.","short":"J.A. Palacci, S. Sacanna, A. Abramian, J. Barral, K. Hanson, A.Y. Grosberg, D.J. Pine, P.M. Chaikin, Science Advances 1 (2015).","apa":"Palacci, J. A., Sacanna, S., Abramian, A., Barral, J., Hanson, K., Grosberg, A. Y., … Chaikin, P. M. (2015). Artificial rheotaxis. <i>Science Advances</i>. American Association for the Advancement of Science . <a href=\"https://doi.org/10.1126/sciadv.1400214\">https://doi.org/10.1126/sciadv.1400214</a>","ieee":"J. A. Palacci <i>et al.</i>, “Artificial rheotaxis,” <i>Science Advances</i>, vol. 1, no. 4. American Association for the Advancement of Science , 2015.","chicago":"Palacci, Jérémie A, Stefano Sacanna, Anaïs Abramian, Jérémie Barral, Kasey Hanson, Alexander Y. Grosberg, David J. Pine, and Paul M. Chaikin. “Artificial Rheotaxis.” <i>Science Advances</i>. American Association for the Advancement of Science , 2015. <a href=\"https://doi.org/10.1126/sciadv.1400214\">https://doi.org/10.1126/sciadv.1400214</a>."},"user_id":"D865714E-FA4E-11E9-B85B-F5C5E5697425","quality_controlled":"1","oa_version":"Published Version","pmid":1,"_id":"9057","extern":"1","publication_identifier":{"issn":["2375-2548"]},"oa":1,"volume":1,"date_updated":"2023-02-23T13:47:52Z","article_processing_charge":"No","arxiv":1}]