---
_id: '624'
abstract:
- lang: eng
  text: Bacteria adapt to adverse environmental conditions by altering gene expression
    patterns. Recently, a novel stress adaptation mechanism has been described that
    allows Escherichia coli to alter gene expression at the post-transcriptional level.
    The key player in this regulatory pathway is the endoribonuclease MazF, the toxin
    component of the toxin-antitoxin module mazEF that is triggered by various stressful
    conditions. In general, MazF degrades the majority of transcripts by cleaving
    at ACA sites, which results in the retardation of bacterial growth. Furthermore,
    MazF can process a small subset of mRNAs and render them leaderless by removing
    their ribosome binding site. MazF concomitantly modifies ribosomes, making them
    selective for the translation of leaderless mRNAs. In this study, we employed
    fluorescent reporter-systems to investigate mazEF expression during stressful
    conditions, and to infer consequences of the mRNA processing mediated by MazF
    on gene expression at the single-cell level. Our results suggest that mazEF transcription
    is maintained at low levels in single cells encountering adverse conditions, such
    as antibiotic stress or amino acid starvation. Moreover, using the grcA mRNA as
    a model for MazF-mediated mRNA processing, we found that MazF activation promotes
    heterogeneity in the grcA reporter expression, resulting in a subpopulation of
    cells with increased levels of GrcA reporter protein.
acknowledgement: 'Austrian Science Fund (FWF): M1697, P22249; Swiss National Science
  Foundation (SNF): 145706; European Commission;FWF Special Research Program: RNA-REG
  F43'
article_number: '3830'
author:
- first_name: Nela
  full_name: Nikolic, Nela
  id: 42D9CABC-F248-11E8-B48F-1D18A9856A87
  last_name: Nikolic
  orcid: 0000-0001-9068-6090
- first_name: Zrinka
  full_name: Didara, Zrinka
  last_name: Didara
- first_name: Isabella
  full_name: Moll, Isabella
  last_name: Moll
citation:
  ama: Nikolic N, Didara Z, Moll I. MazF activation promotes translational heterogeneity
    of the grcA mRNA in Escherichia coli populations. <i>PeerJ</i>. 2017;2017(9).
    doi:<a href="https://doi.org/10.7717/peerj.3830">10.7717/peerj.3830</a>
  apa: Nikolic, N., Didara, Z., &#38; Moll, I. (2017). MazF activation promotes translational
    heterogeneity of the grcA mRNA in Escherichia coli populations. <i>PeerJ</i>.
    PeerJ. <a href="https://doi.org/10.7717/peerj.3830">https://doi.org/10.7717/peerj.3830</a>
  chicago: Nikolic, Nela, Zrinka Didara, and Isabella Moll. “MazF Activation Promotes
    Translational Heterogeneity of the GrcA MRNA in Escherichia Coli Populations.”
    <i>PeerJ</i>. PeerJ, 2017. <a href="https://doi.org/10.7717/peerj.3830">https://doi.org/10.7717/peerj.3830</a>.
  ieee: N. Nikolic, Z. Didara, and I. Moll, “MazF activation promotes translational
    heterogeneity of the grcA mRNA in Escherichia coli populations,” <i>PeerJ</i>,
    vol. 2017, no. 9. PeerJ, 2017.
  ista: Nikolic N, Didara Z, Moll I. 2017. MazF activation promotes translational
    heterogeneity of the grcA mRNA in Escherichia coli populations. PeerJ. 2017(9),
    3830.
  mla: Nikolic, Nela, et al. “MazF Activation Promotes Translational Heterogeneity
    of the GrcA MRNA in Escherichia Coli Populations.” <i>PeerJ</i>, vol. 2017, no.
    9, 3830, PeerJ, 2017, doi:<a href="https://doi.org/10.7717/peerj.3830">10.7717/peerj.3830</a>.
  short: N. Nikolic, Z. Didara, I. Moll, PeerJ 2017 (2017).
date_created: 2018-12-11T11:47:33Z
date_published: 2017-09-21T00:00:00Z
date_updated: 2021-01-12T08:06:48Z
day: '21'
ddc:
- '579'
department:
- _id: CaGu
doi: 10.7717/peerj.3830
file:
- access_level: open_access
  checksum: 3d79ae6b6eabc90b0eaaed82ff3493b0
  content_type: application/pdf
  creator: system
  date_created: 2018-12-12T10:11:51Z
  date_updated: 2020-07-14T12:47:24Z
  file_id: '4908'
  file_name: IST-2017-909-v1+1_peerj-3830.pdf
  file_size: 682064
  relation: main_file
file_date_updated: 2020-07-14T12:47:24Z
has_accepted_license: '1'
intvolume: '      2017'
issue: '9'
language:
- iso: eng
month: '09'
oa: 1
oa_version: Published Version
publication: PeerJ
publication_identifier:
  issn:
  - '21678359'
publication_status: published
publisher: PeerJ
publist_id: '7172'
pubrep_id: '909'
quality_controlled: '1'
scopus_import: 1
status: public
title: MazF activation promotes translational heterogeneity of the grcA mRNA in Escherichia
  coli populations
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 2017
year: '2017'
...