---
_id: '520'
abstract:
- lang: eng
  text: Cyanobacteria are mostly engineered to be sustainable cell-factories by genetic
    manipulations alone. Here, by modulating the concentration of allosteric effectors,
    we focus on increasing product formation without further burdening the cells with
    increased expression of enzymes. Resorting to a novel 96-well microplate cultivation
    system for cyanobacteria, and using lactate-producing strains of Synechocystis
    PCC6803 expressing different l-lactate dehydrogenases (LDH), we titrated the effect
    of 2,5-anhydro-mannitol supplementation. The latter acts in cells as a nonmetabolizable
    analogue of fructose 1,6-bisphosphate, a known allosteric regulator of one of
    the tested LDHs. In this strain (SAA023), we achieved over 2-fold increase of
    lactate productivity. Furthermore, we observed that as carbon is increasingly
    deviated during growth toward product formation, there is an increased fixation
    rate in the population of spontaneous mutants harboring an impaired production
    pathway. This is a challenge in the development of green cell factories, which
    may be countered by the incorporation in biotechnological processes of strategies
    such as the one pioneered here.
article_type: letter_note
author:
- first_name: Wei
  full_name: Du, Wei
  last_name: Du
- first_name: Andreas
  full_name: Angermayr, Andreas
  id: 4677C796-F248-11E8-B48F-1D18A9856A87
  last_name: Angermayr
  orcid: 0000-0001-8619-2223
- first_name: Joeri
  full_name: Jongbloets, Joeri
  last_name: Jongbloets
- first_name: Douwe
  full_name: Molenaar, Douwe
  last_name: Molenaar
- first_name: Herwig
  full_name: Bachmann, Herwig
  last_name: Bachmann
- first_name: Klaas
  full_name: Hellingwerf, Klaas
  last_name: Hellingwerf
- first_name: Filipe
  full_name: Branco Dos Santos, Filipe
  last_name: Branco Dos Santos
citation:
  ama: Du W, Angermayr A, Jongbloets J, et al. Nonhierarchical flux regulation exposes
    the fitness burden associated with lactate production in Synechocystis sp. PCC6803.
    <i>ACS Synthetic Biology</i>. 2017;6(3):395-401. doi:<a href="https://doi.org/10.1021/acssynbio.6b00235">10.1021/acssynbio.6b00235</a>
  apa: Du, W., Angermayr, A., Jongbloets, J., Molenaar, D., Bachmann, H., Hellingwerf,
    K., &#38; Branco Dos Santos, F. (2017). Nonhierarchical flux regulation exposes
    the fitness burden associated with lactate production in Synechocystis sp. PCC6803.
    <i>ACS Synthetic Biology</i>. American Chemical Society. <a href="https://doi.org/10.1021/acssynbio.6b00235">https://doi.org/10.1021/acssynbio.6b00235</a>
  chicago: Du, Wei, Andreas Angermayr, Joeri Jongbloets, Douwe Molenaar, Herwig Bachmann,
    Klaas Hellingwerf, and Filipe Branco Dos Santos. “Nonhierarchical Flux Regulation
    Exposes the Fitness Burden Associated with Lactate Production in Synechocystis
    Sp. PCC6803.” <i>ACS Synthetic Biology</i>. American Chemical Society, 2017. <a
    href="https://doi.org/10.1021/acssynbio.6b00235">https://doi.org/10.1021/acssynbio.6b00235</a>.
  ieee: W. Du <i>et al.</i>, “Nonhierarchical flux regulation exposes the fitness
    burden associated with lactate production in Synechocystis sp. PCC6803,” <i>ACS
    Synthetic Biology</i>, vol. 6, no. 3. American Chemical Society, pp. 395–401,
    2017.
  ista: Du W, Angermayr A, Jongbloets J, Molenaar D, Bachmann H, Hellingwerf K, Branco
    Dos Santos F. 2017. Nonhierarchical flux regulation exposes the fitness burden
    associated with lactate production in Synechocystis sp. PCC6803. ACS Synthetic
    Biology. 6(3), 395–401.
  mla: Du, Wei, et al. “Nonhierarchical Flux Regulation Exposes the Fitness Burden
    Associated with Lactate Production in Synechocystis Sp. PCC6803.” <i>ACS Synthetic
    Biology</i>, vol. 6, no. 3, American Chemical Society, 2017, pp. 395–401, doi:<a
    href="https://doi.org/10.1021/acssynbio.6b00235">10.1021/acssynbio.6b00235</a>.
  short: W. Du, A. Angermayr, J. Jongbloets, D. Molenaar, H. Bachmann, K. Hellingwerf,
    F. Branco Dos Santos, ACS Synthetic Biology 6 (2017) 395–401.
date_created: 2018-12-11T11:46:56Z
date_published: 2017-03-17T00:00:00Z
date_updated: 2021-01-12T08:01:21Z
day: '17'
department:
- _id: ToBo
doi: 10.1021/acssynbio.6b00235
external_id:
  pmid:
  - '27936615'
intvolume: '         6'
issue: '3'
language:
- iso: eng
month: '03'
oa_version: None
page: 395 - 401
pmid: 1
publication: ACS Synthetic Biology
publication_identifier:
  issn:
  - '21615063'
publication_status: published
publisher: American Chemical Society
publist_id: '7298'
quality_controlled: '1'
scopus_import: 1
status: public
title: Nonhierarchical flux regulation exposes the fitness burden associated with
  lactate production in Synechocystis sp. PCC6803
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 6
year: '2017'
...