---
_id: '14077'
abstract:
- lang: eng
  text: "The regulatory architecture of gene expression is known to differ substantially
    between sexes in Drosophila, but most studies performed\r\nso far used whole-body
    data and only single crosses, which may have limited their scope to detect patterns
    that are robust across tissues\r\nand biological replicates. Here, we use allele-specific
    gene expression of parental and reciprocal hybrid crosses between 6 Drosophila\r\nmelanogaster
    inbred lines to quantify cis- and trans-regulatory variation in heads and gonads
    of both sexes separately across 3 replicate\r\ncrosses. Our results suggest that
    female and male heads, as well as ovaries, have a similar regulatory architecture.
    On the other hand,\r\ntestes display more and substantially different cis-regulatory
    effects, suggesting that sex differences in the regulatory architecture that\r\nhave
    been previously observed may largely derive from testis-specific effects. We also
    examine the difference in cis-regulatory variation\r\nof genes across different
    levels of sex bias in gonads and heads. Consistent with the idea that intersex
    correlations constrain expression\r\nand can lead to sexual antagonism, we find
    more cis variation in unbiased and moderately biased genes in heads. In ovaries,
    reduced cis\r\nvariation is observed for male-biased genes, suggesting that cis
    variants acting on these genes in males do not lead to changes in ovary\r\nexpression.
    Finally, we examine the dominance patterns of gene expression and find that sex-
    and tissue-specific patterns of inheritance\r\nas well as trans-regulatory variation
    are highly variable across biological crosses, although these were performed in
    highly controlled\r\nexperimental conditions. This highlights the importance of
    using various genetic backgrounds to infer generalizable patterns."
acknowledged_ssus:
- _id: ScienComp
acknowledgement: We thank members of the Vicoso Group for comments on the manuscript,
  the Scientific Computing Unit at ISTA for technical support, and 2 anonymous reviewers
  for useful feedback. GP is the recipient of a DOC Fellowship of the Austrian Academy
  of Sciences at the Institute of Science and Technology Austria (DOC 25817) and received
  funding from the European Union’s Horizon 2020 research and innovation program under
  the Marie Skłodowska-Curie Grant (agreement no. 665385).
article_processing_charge: Yes
article_type: original
author:
- first_name: Gemma
  full_name: Puixeu Sala, Gemma
  id: 33AB266C-F248-11E8-B48F-1D18A9856A87
  last_name: Puixeu Sala
  orcid: 0000-0001-8330-1754
- first_name: Ariana
  full_name: Macon, Ariana
  id: 2A0848E2-F248-11E8-B48F-1D18A9856A87
  last_name: Macon
- first_name: Beatriz
  full_name: Vicoso, Beatriz
  id: 49E1C5C6-F248-11E8-B48F-1D18A9856A87
  last_name: Vicoso
  orcid: 0000-0002-4579-8306
citation:
  ama: 'Puixeu Sala G, Macon A, Vicoso B. Sex-specific estimation of cis and trans
    regulation of gene expression in heads and gonads of Drosophila melanogaster.
    <i>G3: Genes, Genomes, Genetics</i>. 2023;13(8). doi:<a href="https://doi.org/10.1093/g3journal/jkad121">10.1093/g3journal/jkad121</a>'
  apa: 'Puixeu Sala, G., Macon, A., &#38; Vicoso, B. (2023). Sex-specific estimation
    of cis and trans regulation of gene expression in heads and gonads of Drosophila
    melanogaster. <i>G3: Genes, Genomes, Genetics</i>. Oxford University Press. <a
    href="https://doi.org/10.1093/g3journal/jkad121">https://doi.org/10.1093/g3journal/jkad121</a>'
  chicago: 'Puixeu Sala, Gemma, Ariana Macon, and Beatriz Vicoso. “Sex-Specific Estimation
    of Cis and Trans Regulation of Gene Expression in Heads and Gonads of Drosophila
    Melanogaster.” <i>G3: Genes, Genomes, Genetics</i>. Oxford University Press, 2023.
    <a href="https://doi.org/10.1093/g3journal/jkad121">https://doi.org/10.1093/g3journal/jkad121</a>.'
  ieee: 'G. Puixeu Sala, A. Macon, and B. Vicoso, “Sex-specific estimation of cis
    and trans regulation of gene expression in heads and gonads of Drosophila melanogaster,”
    <i>G3: Genes, Genomes, Genetics</i>, vol. 13, no. 8. Oxford University Press,
    2023.'
  ista: 'Puixeu Sala G, Macon A, Vicoso B. 2023. Sex-specific estimation of cis and
    trans regulation of gene expression in heads and gonads of Drosophila melanogaster.
    G3: Genes, Genomes, Genetics. 13(8).'
  mla: 'Puixeu Sala, Gemma, et al. “Sex-Specific Estimation of Cis and Trans Regulation
    of Gene Expression in Heads and Gonads of Drosophila Melanogaster.” <i>G3: Genes,
    Genomes, Genetics</i>, vol. 13, no. 8, Oxford University Press, 2023, doi:<a href="https://doi.org/10.1093/g3journal/jkad121">10.1093/g3journal/jkad121</a>.'
  short: 'G. Puixeu Sala, A. Macon, B. Vicoso, G3: Genes, Genomes, Genetics 13 (2023).'
date_created: 2023-08-18T06:52:14Z
date_published: 2023-08-01T00:00:00Z
date_updated: 2023-12-13T12:15:37Z
day: '01'
ddc:
- '570'
department:
- _id: BeVi
- _id: NiBa
- _id: GradSch
doi: 10.1093/g3journal/jkad121
ec_funded: 1
external_id:
  isi:
  - '001002997200001'
file:
- access_level: open_access
  checksum: c62e29fc7c5efbf8356f4c60cab4a2d1
  content_type: application/pdf
  creator: dernst
  date_created: 2023-11-07T09:00:19Z
  date_updated: 2023-11-07T09:00:19Z
  file_id: '14498'
  file_name: 2023_G3_Puixeu.pdf
  file_size: 845642
  relation: main_file
  success: 1
file_date_updated: 2023-11-07T09:00:19Z
has_accepted_license: '1'
intvolume: '        13'
isi: 1
issue: '8'
keyword:
- Genetics (clinical)
- Genetics
- Molecular Biology
language:
- iso: eng
license: https://creativecommons.org/licenses/by/4.0/
month: '08'
oa: 1
oa_version: Published Version
project:
- _id: 2564DBCA-B435-11E9-9278-68D0E5697425
  call_identifier: H2020
  grant_number: '665385'
  name: International IST Doctoral Program
- _id: 9B9DFC9E-BA93-11EA-9121-9846C619BF3A
  grant_number: '25817'
  name: 'Sexual conflict: resolution, constraints and biomedical implications'
publication: 'G3: Genes, Genomes, Genetics'
publication_identifier:
  issn:
  - 2160-1836
publication_status: published
publisher: Oxford University Press
quality_controlled: '1'
related_material:
  record:
  - id: '12933'
    relation: research_data
    status: public
  - id: '14058'
    relation: dissertation_contains
    status: public
scopus_import: '1'
status: public
title: Sex-specific estimation of cis and trans regulation of gene expression in heads
  and gonads of Drosophila melanogaster
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 13
year: '2023'
...
---
_id: '7547'
abstract:
- lang: eng
  text: The BH3-only family of proteins is key for initiating apoptosis in a variety
    of contexts, and may also contribute to non-apoptotic cellular processes. Historically,
    the nematode Caenorhabditis elegans has provided a powerful system for studying
    and identifying conserved regulators of BH3-only proteins. In C. elegans, the
    BH3-only protein egl-1 is expressed during development to cell-autonomously trigger
    most developmental cell deaths. Here we provide evidence that egl-1 is also transcribed
    after development in the sensory neuron pair URX without inducing apoptosis. We
    used genetic screening and epistasis analysis to determine that its transcription
    is regulated in URX by neuronal activity and/or in parallel by orthologs of Protein
    Kinase G and the Salt-Inducible Kinase family. Because several BH3-only family
    proteins are also expressed in the adult nervous system of mammals, we suggest
    that studying egl-1 expression in URX may shed light on mechanisms that regulate
    conserved family members in higher organisms.
article_processing_charge: No
article_type: original
author:
- first_name: Jesse
  full_name: Cohn, Jesse
  last_name: Cohn
- first_name: Vivek
  full_name: Dwivedi, Vivek
  last_name: Dwivedi
- first_name: Giulio
  full_name: Valperga, Giulio
  last_name: Valperga
- first_name: Nicole
  full_name: Zarate, Nicole
  last_name: Zarate
- first_name: Mario
  full_name: de Bono, Mario
  id: 4E3FF80E-F248-11E8-B48F-1D18A9856A87
  last_name: de Bono
  orcid: 0000-0001-8347-0443
- first_name: H. Robert
  full_name: Horvitz, H. Robert
  last_name: Horvitz
- first_name: Jonathan T.
  full_name: Pierce, Jonathan T.
  last_name: Pierce
citation:
  ama: 'Cohn J, Dwivedi V, Valperga G, et al. Activity-dependent regulation of the
    proapoptotic BH3-only gene egl-1 in a living neuron pair in Caenorhabditis elegans.
    <i>G3: Genes, Genomes, Genetics</i>. 2019;9(11):3703-3714. doi:<a href="https://doi.org/10.1534/g3.119.400654">10.1534/g3.119.400654</a>'
  apa: 'Cohn, J., Dwivedi, V., Valperga, G., Zarate, N., de Bono, M., Horvitz, H.
    R., &#38; Pierce, J. T. (2019). Activity-dependent regulation of the proapoptotic
    BH3-only gene egl-1 in a living neuron pair in Caenorhabditis elegans. <i>G3:
    Genes, Genomes, Genetics</i>. Genetics Society of America. <a href="https://doi.org/10.1534/g3.119.400654">https://doi.org/10.1534/g3.119.400654</a>'
  chicago: 'Cohn, Jesse, Vivek Dwivedi, Giulio Valperga, Nicole Zarate, Mario de Bono,
    H. Robert Horvitz, and Jonathan T. Pierce. “Activity-Dependent Regulation of the
    Proapoptotic BH3-Only Gene Egl-1 in a Living Neuron Pair in Caenorhabditis Elegans.”
    <i>G3: Genes, Genomes, Genetics</i>. Genetics Society of America, 2019. <a href="https://doi.org/10.1534/g3.119.400654">https://doi.org/10.1534/g3.119.400654</a>.'
  ieee: 'J. Cohn <i>et al.</i>, “Activity-dependent regulation of the proapoptotic
    BH3-only gene egl-1 in a living neuron pair in Caenorhabditis elegans,” <i>G3:
    Genes, Genomes, Genetics</i>, vol. 9, no. 11. Genetics Society of America, pp.
    3703–3714, 2019.'
  ista: 'Cohn J, Dwivedi V, Valperga G, Zarate N, de Bono M, Horvitz HR, Pierce JT.
    2019. Activity-dependent regulation of the proapoptotic BH3-only gene egl-1 in
    a living neuron pair in Caenorhabditis elegans. G3: Genes, Genomes, Genetics.
    9(11), 3703–3714.'
  mla: 'Cohn, Jesse, et al. “Activity-Dependent Regulation of the Proapoptotic BH3-Only
    Gene Egl-1 in a Living Neuron Pair in Caenorhabditis Elegans.” <i>G3: Genes, Genomes,
    Genetics</i>, vol. 9, no. 11, Genetics Society of America, 2019, pp. 3703–14,
    doi:<a href="https://doi.org/10.1534/g3.119.400654">10.1534/g3.119.400654</a>.'
  short: 'J. Cohn, V. Dwivedi, G. Valperga, N. Zarate, M. de Bono, H.R. Horvitz, J.T.
    Pierce, G3: Genes, Genomes, Genetics 9 (2019) 3703–3714.'
date_created: 2020-02-28T10:44:27Z
date_published: 2019-11-01T00:00:00Z
date_updated: 2021-01-12T08:14:07Z
day: '01'
doi: 10.1534/g3.119.400654
extern: '1'
external_id:
  pmid:
  - '31519744'
intvolume: '         9'
issue: '11'
language:
- iso: eng
month: '11'
oa_version: Published Version
page: 3703-3714
pmid: 1
publication: 'G3: Genes, Genomes, Genetics'
publication_identifier:
  issn:
  - 2160-1836
publication_status: published
publisher: Genetics Society of America
quality_controlled: '1'
status: public
title: Activity-dependent regulation of the proapoptotic BH3-only gene egl-1 in a
  living neuron pair in Caenorhabditis elegans
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 9
year: '2019'
...