---
_id: '7387'
abstract:
- lang: eng
  text: Most bacteria accomplish cell division with the help of a dynamic protein
    complex called the divisome, which spans the cell envelope in the plane of division.
    Assembly and activation of this machinery are coordinated by the tubulin-related
    GTPase FtsZ, which was found to form treadmilling filaments on supported bilayers
    in vitro1, as well as in live cells, in which filaments circle around the cell
    division site2,3. Treadmilling of FtsZ is thought to actively move proteins around
    the division septum, thereby distributing peptidoglycan synthesis and coordinating
    the inward growth of the septum to form the new poles of the daughter cells4.
    However, the molecular mechanisms underlying this function are largely unknown.
    Here, to study how FtsZ polymerization dynamics are coupled to downstream proteins,
    we reconstituted part of the bacterial cell division machinery using its purified
    components FtsZ, FtsA and truncated transmembrane proteins essential for cell
    division. We found that the membrane-bound cytosolic peptides of FtsN and FtsQ
    co-migrated with treadmilling FtsZ–FtsA filaments, but despite their directed
    collective behaviour, individual peptides showed random motion and transient confinement.
    Our work suggests that divisome proteins follow treadmilling FtsZ filaments by
    a diffusion-and-capture mechanism, which can give rise to a moving zone of signalling
    activity at the division site.
acknowledgement: We acknowledge members of the Loose laboratory at IST Austria for
  helpful discussions—in particular, P. Caldas for help with the treadmilling analysis,
  M. Jimenez, A. Raso and N. Ropero for providing Alexa Fluor 488- and Alexa Fluor
  647-labelled FtsA for the MST and analytical ultracentrifugation experiments. We
  thank C. You for providing the DODA-tris-NTA phospholipids, as well as J. Piehler
  and C. Richter (Department of Biology, University of Osnabruck, Germany) for the
  SLIMfast single-molecule tracking software and help with the confinement analysis.
  We thank J. Errington and H. Murray (both at Newcastle University, UK) for critical
  reading of the manuscript, and J. Brugués (MPI-CBG and MPI-PKS, Dresden, Germany)
  for help with the MATLAB programming and reading of the manuscript. This work was
  supported by the European Research Council through grant ERC-2015-StG-679239 to
  M.L. and grants HFSP LT 000824/2016-L4 and EMBO ALTF 1163-2015 to N.B., a grant
  from the Ministry of Economy and Competitiveness of the Spanish Government (BFU2016-75471-C2-1-P)
  to C.A. and G.R., and a Wellcome Trust Senior Investigator award (101824/Z/13/Z)
  and a grant from the BBSRC (BB/R017409/1) to W.V.
article_processing_charge: No
article_type: letter_note
author:
- first_name: Natalia S.
  full_name: Baranova, Natalia S.
  id: 38661662-F248-11E8-B48F-1D18A9856A87
  last_name: Baranova
  orcid: 0000-0002-3086-9124
- first_name: Philipp
  full_name: Radler, Philipp
  id: 40136C2A-F248-11E8-B48F-1D18A9856A87
  last_name: Radler
  orcid: '0000-0001-9198-2182 '
- first_name: Víctor M.
  full_name: Hernández-Rocamora, Víctor M.
  last_name: Hernández-Rocamora
- first_name: Carlos
  full_name: Alfonso, Carlos
  last_name: Alfonso
- first_name: Maria D
  full_name: Lopez Pelegrin, Maria D
  id: 319AA9CE-F248-11E8-B48F-1D18A9856A87
  last_name: Lopez Pelegrin
- first_name: Germán
  full_name: Rivas, Germán
  last_name: Rivas
- first_name: Waldemar
  full_name: Vollmer, Waldemar
  last_name: Vollmer
- first_name: Martin
  full_name: Loose, Martin
  id: 462D4284-F248-11E8-B48F-1D18A9856A87
  last_name: Loose
  orcid: 0000-0001-7309-9724
citation:
  ama: Baranova NS, Radler P, Hernández-Rocamora VM, et al. Diffusion and capture
    permits dynamic coupling between treadmilling FtsZ filaments and cell division
    proteins. <i>Nature Microbiology</i>. 2020;5:407-417. doi:<a href="https://doi.org/10.1038/s41564-019-0657-5">10.1038/s41564-019-0657-5</a>
  apa: Baranova, N. S., Radler, P., Hernández-Rocamora, V. M., Alfonso, C., Lopez
    Pelegrin, M. D., Rivas, G., … Loose, M. (2020). Diffusion and capture permits
    dynamic coupling between treadmilling FtsZ filaments and cell division proteins.
    <i>Nature Microbiology</i>. Springer Nature. <a href="https://doi.org/10.1038/s41564-019-0657-5">https://doi.org/10.1038/s41564-019-0657-5</a>
  chicago: Baranova, Natalia S., Philipp Radler, Víctor M. Hernández-Rocamora, Carlos
    Alfonso, Maria D Lopez Pelegrin, Germán Rivas, Waldemar Vollmer, and Martin Loose.
    “Diffusion and Capture Permits Dynamic Coupling between Treadmilling FtsZ Filaments
    and Cell Division Proteins.” <i>Nature Microbiology</i>. Springer Nature, 2020.
    <a href="https://doi.org/10.1038/s41564-019-0657-5">https://doi.org/10.1038/s41564-019-0657-5</a>.
  ieee: N. S. Baranova <i>et al.</i>, “Diffusion and capture permits dynamic coupling
    between treadmilling FtsZ filaments and cell division proteins,” <i>Nature Microbiology</i>,
    vol. 5. Springer Nature, pp. 407–417, 2020.
  ista: Baranova NS, Radler P, Hernández-Rocamora VM, Alfonso C, Lopez Pelegrin MD,
    Rivas G, Vollmer W, Loose M. 2020. Diffusion and capture permits dynamic coupling
    between treadmilling FtsZ filaments and cell division proteins. Nature Microbiology.
    5, 407–417.
  mla: Baranova, Natalia S., et al. “Diffusion and Capture Permits Dynamic Coupling
    between Treadmilling FtsZ Filaments and Cell Division Proteins.” <i>Nature Microbiology</i>,
    vol. 5, Springer Nature, 2020, pp. 407–17, doi:<a href="https://doi.org/10.1038/s41564-019-0657-5">10.1038/s41564-019-0657-5</a>.
  short: N.S. Baranova, P. Radler, V.M. Hernández-Rocamora, C. Alfonso, M.D. Lopez
    Pelegrin, G. Rivas, W. Vollmer, M. Loose, Nature Microbiology 5 (2020) 407–417.
date_created: 2020-01-28T16:14:41Z
date_published: 2020-01-20T00:00:00Z
date_updated: 2023-10-06T12:22:38Z
day: '20'
department:
- _id: MaLo
doi: 10.1038/s41564-019-0657-5
ec_funded: 1
external_id:
  isi:
  - '000508584700007'
  pmid:
  - '31959972'
intvolume: '         5'
isi: 1
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: http://europepmc.org/article/PMC/7048620
month: '01'
oa: 1
oa_version: Submitted Version
page: 407-417
pmid: 1
project:
- _id: 2595697A-B435-11E9-9278-68D0E5697425
  call_identifier: H2020
  grant_number: '679239'
  name: Self-Organization of the Bacterial Cell
- _id: 259B655A-B435-11E9-9278-68D0E5697425
  grant_number: LT000824/2016
  name: Reconstitution of bacterial cell wall sythesis
- _id: 2596EAB6-B435-11E9-9278-68D0E5697425
  grant_number: ALTF 2015-1163
  name: Synthesis of bacterial cell wall
publication: Nature Microbiology
publication_identifier:
  issn:
  - 2058-5276
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
related_material:
  link:
  - description: News on IST Homepage
    relation: press_release
    url: https://ist.ac.at/en/news/little-cell-big-cover-story/
  record:
  - id: '14280'
    relation: dissertation_contains
    status: public
scopus_import: '1'
status: public
title: Diffusion and capture permits dynamic coupling between treadmilling FtsZ filaments
  and cell division proteins
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 5
year: '2020'
...
---
_id: '6506'
abstract:
- lang: eng
  text: How does environmental complexity affect the evolution of single genes? Here,
    we measured the effects of a set of Bacillus subtilis glutamate dehydrogenase
    mutants across 19 different environments—from phenotypically homogeneous single-cell
    populations in liquid media to heterogeneous biofilms, plant roots and soil populations.
    The effects of individual gene mutations on organismal fitness were highly reproducible
    in liquid cultures. However, 84% of the tested alleles showed opposing fitness
    effects under different growth conditions (sign environmental pleiotropy). In
    colony biofilms and soil samples, different alleles dominated in parallel replica
    experiments. Accordingly, we found that in these heterogeneous cell populations
    the fate of mutations was dictated by a combination of selection and drift. The
    latter relates to programmed prophage excisions that occurred during biofilm development.
    Overall, for each condition, a wide range of glutamate dehydrogenase mutations
    persisted and sometimes fixated as a result of the combined action of selection,
    pleiotropy and chance. However, over longer periods and in multiple environments,
    nearly all of this diversity would be lost—across all the environments and conditions
    that we tested, the wild type was the fittest allele.
article_processing_charge: No
article_type: original
author:
- first_name: Lianet
  full_name: Noda-García, Lianet
  last_name: Noda-García
- first_name: Dan
  full_name: Davidi, Dan
  last_name: Davidi
- first_name: Elisa
  full_name: Korenblum, Elisa
  last_name: Korenblum
- first_name: Assaf
  full_name: Elazar, Assaf
  last_name: Elazar
- first_name: Ekaterina
  full_name: Putintseva, Ekaterina
  id: 2EF67C84-F248-11E8-B48F-1D18A9856A87
  last_name: Putintseva
- first_name: Asaph
  full_name: Aharoni, Asaph
  last_name: Aharoni
- first_name: Dan S.
  full_name: Tawfik, Dan S.
  last_name: Tawfik
citation:
  ama: Noda-García L, Davidi D, Korenblum E, et al. Chance and pleiotropy dominate
    genetic diversity in complex bacterial environments. <i>Nature Microbiology</i>.
    2019;4(7):1221–1230. doi:<a href="https://doi.org/10.1038/s41564-019-0412-y">10.1038/s41564-019-0412-y</a>
  apa: Noda-García, L., Davidi, D., Korenblum, E., Elazar, A., Putintseva, E., Aharoni,
    A., &#38; Tawfik, D. S. (2019). Chance and pleiotropy dominate genetic diversity
    in complex bacterial environments. <i>Nature Microbiology</i>. Springer Nature.
    <a href="https://doi.org/10.1038/s41564-019-0412-y">https://doi.org/10.1038/s41564-019-0412-y</a>
  chicago: Noda-García, Lianet, Dan Davidi, Elisa Korenblum, Assaf Elazar, Ekaterina
    Putintseva, Asaph Aharoni, and Dan S. Tawfik. “Chance and Pleiotropy Dominate
    Genetic Diversity in Complex Bacterial Environments.” <i>Nature Microbiology</i>.
    Springer Nature, 2019. <a href="https://doi.org/10.1038/s41564-019-0412-y">https://doi.org/10.1038/s41564-019-0412-y</a>.
  ieee: L. Noda-García <i>et al.</i>, “Chance and pleiotropy dominate genetic diversity
    in complex bacterial environments,” <i>Nature Microbiology</i>, vol. 4, no. 7.
    Springer Nature, pp. 1221–1230, 2019.
  ista: Noda-García L, Davidi D, Korenblum E, Elazar A, Putintseva E, Aharoni A, Tawfik
    DS. 2019. Chance and pleiotropy dominate genetic diversity in complex bacterial
    environments. Nature Microbiology. 4(7), 1221–1230.
  mla: Noda-García, Lianet, et al. “Chance and Pleiotropy Dominate Genetic Diversity
    in Complex Bacterial Environments.” <i>Nature Microbiology</i>, vol. 4, no. 7,
    Springer Nature, 2019, pp. 1221–1230, doi:<a href="https://doi.org/10.1038/s41564-019-0412-y">10.1038/s41564-019-0412-y</a>.
  short: L. Noda-García, D. Davidi, E. Korenblum, A. Elazar, E. Putintseva, A. Aharoni,
    D.S. Tawfik, Nature Microbiology 4 (2019) 1221–1230.
date_created: 2019-05-29T13:03:30Z
date_published: 2019-07-01T00:00:00Z
date_updated: 2023-08-28T08:39:47Z
day: '01'
department:
- _id: FyKo
doi: 10.1038/s41564-019-0412-y
external_id:
  isi:
  - '000480348200017'
intvolume: '         4'
isi: 1
issue: '7'
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: https://www.biorxiv.org/content/10.1101/340828v2
month: '07'
oa: 1
oa_version: Preprint
page: 1221–1230
publication: Nature Microbiology
publication_identifier:
  issn:
  - 2058-5276
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
status: public
title: Chance and pleiotropy dominate genetic diversity in complex bacterial environments
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 4
year: '2019'
...