--- _id: '8235' abstract: - lang: eng text: Due to large homology of human and canine EGFR, dogs suffering from spontaneous EGFR+ cancer can be considered as ideal translational models. Thereby, novel immunotherapeutic compounds can be developed for both human and veterinary patients. This study describes the radiolabeling of a canine anti-EGFR IgG antibody (can225IgG) with potential diagnostic and therapeutic value in comparative clinical settings. Can225IgG was functionalized with DTPA for subsequent chelation with the radionuclide 99mTc. Successful coupling of 10 DTPA molecules per antibody on average was proven by significant mass increase in MALDI-TOF spectroscopy, gel electrophoresis and immunoblots. Following functionalization and radiolabeling, 99mTc-DTPA-can225IgG fully retained its binding capacity towards human and canine EGFR in flow cytometry, immuno- and radioblots, and autoradiography. The affinity of radiolabeled can225IgG was determined to KD 0.8 ±0.0031 nM in a real-time kinetics assay on canine carcinoma cells by a competition binding technique. Stability tests of the radiolabeled compound identified TRIS buffered saline as the ideal formulation for short-term storage with 87.11 ±6.04% intact compound being still detected 60 minutes post radiolabeling. High stability, specificity and EGFR binding affinity pinpoint towards 99mTc-radiolabeled can225IgG antibody as an ideal lead compound for the first proof-of-concept diagnostic and therapeutic applications in canine cancer patients. article_processing_charge: No article_type: original author: - first_name: Judit full_name: Fazekas-Singer, Judit id: 36432834-F248-11E8-B48F-1D18A9856A87 last_name: Fazekas-Singer orcid: 0000-0002-8777-3502 - first_name: Neydher full_name: Berroterán-Infante, Neydher last_name: Berroterán-Infante - first_name: Christina full_name: Rami-Mark, Christina last_name: Rami-Mark - first_name: Monika full_name: Dumanic, Monika last_name: Dumanic - first_name: Miroslawa full_name: Matz, Miroslawa last_name: Matz - first_name: Michael full_name: Willmann, Michael last_name: Willmann - first_name: Fritz full_name: Andreae, Fritz last_name: Andreae - first_name: Josef full_name: Singer, Josef last_name: Singer - first_name: Wolfgang full_name: Wadsak, Wolfgang last_name: Wadsak - first_name: Markus full_name: Mitterhauser, Markus last_name: Mitterhauser - first_name: Erika full_name: Jensen-Jarolim, Erika last_name: Jensen-Jarolim citation: ama: Singer J, Berroterán-Infante N, Rami-Mark C, et al. Development of a radiolabeled caninized anti-EGFR antibody for comparative oncology trials. Oncotarget. 2017;8:83128-83141. doi:10.18632/oncotarget.20914 apa: Singer, J., Berroterán-Infante, N., Rami-Mark, C., Dumanic, M., Matz, M., Willmann, M., … Jensen-Jarolim, E. (2017). Development of a radiolabeled caninized anti-EGFR antibody for comparative oncology trials. Oncotarget. Impact Journals. https://doi.org/10.18632/oncotarget.20914 chicago: Singer, Judit, Neydher Berroterán-Infante, Christina Rami-Mark, Monika Dumanic, Miroslawa Matz, Michael Willmann, Fritz Andreae, et al. “Development of a Radiolabeled Caninized Anti-EGFR Antibody for Comparative Oncology Trials.” Oncotarget. Impact Journals, 2017. https://doi.org/10.18632/oncotarget.20914. ieee: J. Singer et al., “Development of a radiolabeled caninized anti-EGFR antibody for comparative oncology trials,” Oncotarget, vol. 8. Impact Journals, pp. 83128–83141, 2017. ista: Singer J, Berroterán-Infante N, Rami-Mark C, Dumanic M, Matz M, Willmann M, Andreae F, Singer J, Wadsak W, Mitterhauser M, Jensen-Jarolim E. 2017. Development of a radiolabeled caninized anti-EGFR antibody for comparative oncology trials. Oncotarget. 8, 83128–83141. mla: Singer, Judit, et al. “Development of a Radiolabeled Caninized Anti-EGFR Antibody for Comparative Oncology Trials.” Oncotarget, vol. 8, Impact Journals, 2017, pp. 83128–41, doi:10.18632/oncotarget.20914. short: J. Singer, N. Berroterán-Infante, C. Rami-Mark, M. Dumanic, M. Matz, M. Willmann, F. Andreae, J. Singer, W. Wadsak, M. Mitterhauser, E. Jensen-Jarolim, Oncotarget 8 (2017) 83128–83141. date_created: 2020-08-10T11:53:18Z date_published: 2017-09-15T00:00:00Z date_updated: 2021-01-12T08:17:39Z day: '15' doi: 10.18632/oncotarget.20914 extern: '1' intvolume: ' 8' language: - iso: eng main_file_link: - open_access: '1' url: https://doi.org/10.18632/oncotarget.20914 month: '09' oa: 1 oa_version: Published Version page: 83128-83141 publication: Oncotarget publication_identifier: issn: - 1949-2553 publication_status: published publisher: Impact Journals quality_controlled: '1' status: public title: Development of a radiolabeled caninized anti-EGFR antibody for comparative oncology trials type: journal_article user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87 volume: 8 year: '2017' ... --- _id: '8240' abstract: - lang: eng text: "Background/Aim: Cancer cell lines are indispensible surrogate models in cancer research, as they can be used off-the-shelf, expanded to the desired extent, easily modified and exchanged between research groups for affirmation, reproduction or follow-up experiments.\r\nAs malignant cells are prone to genomic instability, phenotypical changes may occur after certain passages in culture. Thus, cell lines have to be regularly authenticated to ensure data quality. In between experiments these cell lines are often stored in liquid nitrogen for extended time periods.\r\nAlthough freezing of cells is a necessary evil, little research is performed on how long-term storage affects cancer cell lines. Therefore, this study investigated the effects of a 28-year long liquid nitrogen storage period on BT474 cells with regard to phenotypical changes, differences in cell-surface receptor expression as well as cytokine and gene expressional variations.\r\nMethods: Two batches of BT474 cells, one frozen in 1986, the other directly purchased from ATCC were investigated by light microscopy, cell growth analysis, flow cytometry and cytokine as well as whole-transcriptome expression profiling.\r\nResults: The cell lines were morphologically indifferent and showed similar growth rates and similar cell-surface receptor expression. Transcriptome analysis revealed significant differences in only 26 of 40,716 investigated RefSeq transcripts with 4 of them being up-regulated and 22 down-regulated.\r\nConclusion: This study demonstrates that even after very long periods of storage in liquid nitrogen, cancer cell lines display only minimal changes in their gene expression profiles. However, also such minor changes should be carefully assessed before continuation of experiments, especially if phenotypic alterations can be additionally observed." article_processing_charge: No article_type: original author: - first_name: Judit full_name: Fazekas, Judit id: 36432834-F248-11E8-B48F-1D18A9856A87 last_name: Fazekas orcid: 0000-0002-8777-3502 - first_name: Thomas W. full_name: Grunt, Thomas W. last_name: Grunt - first_name: Erika full_name: Jensen-Jarolim, Erika last_name: Jensen-Jarolim - first_name: Josef full_name: Singer, Josef last_name: Singer citation: ama: Singer J, Grunt TW, Jensen-Jarolim E, Singer J. Long term storage in liquid nitrogen leads to only minor phenotypic and gene expression changes in the mammary carcinoma model cell line BT474. Oncotarget. 2017;8:35076-35087. doi:10.18632/oncotarget.16623 apa: Singer, J., Grunt, T. W., Jensen-Jarolim, E., & Singer, J. (2017). Long term storage in liquid nitrogen leads to only minor phenotypic and gene expression changes in the mammary carcinoma model cell line BT474. Oncotarget. Impact Journals. https://doi.org/10.18632/oncotarget.16623 chicago: Singer, Judit, Thomas W. Grunt, Erika Jensen-Jarolim, and Josef Singer. “Long Term Storage in Liquid Nitrogen Leads to Only Minor Phenotypic and Gene Expression Changes in the Mammary Carcinoma Model Cell Line BT474.” Oncotarget. Impact Journals, 2017. https://doi.org/10.18632/oncotarget.16623. ieee: J. Singer, T. W. Grunt, E. Jensen-Jarolim, and J. Singer, “Long term storage in liquid nitrogen leads to only minor phenotypic and gene expression changes in the mammary carcinoma model cell line BT474,” Oncotarget, vol. 8. Impact Journals, pp. 35076–35087, 2017. ista: Singer J, Grunt TW, Jensen-Jarolim E, Singer J. 2017. Long term storage in liquid nitrogen leads to only minor phenotypic and gene expression changes in the mammary carcinoma model cell line BT474. Oncotarget. 8, 35076–35087. mla: Singer, Judit, et al. “Long Term Storage in Liquid Nitrogen Leads to Only Minor Phenotypic and Gene Expression Changes in the Mammary Carcinoma Model Cell Line BT474.” Oncotarget, vol. 8, Impact Journals, 2017, pp. 35076–87, doi:10.18632/oncotarget.16623. short: J. Singer, T.W. Grunt, E. Jensen-Jarolim, J. Singer, Oncotarget 8 (2017) 35076–35087. date_created: 2020-08-10T11:53:53Z date_published: 2017-03-28T00:00:00Z date_updated: 2021-01-12T08:17:41Z day: '28' doi: 10.18632/oncotarget.16623 extern: '1' intvolume: ' 8' language: - iso: eng main_file_link: - open_access: '1' url: https://doi.org/10.18632/oncotarget.16623 month: '03' oa: 1 oa_version: Published Version page: 35076-35087 publication: Oncotarget publication_identifier: issn: - 1949-2553 publication_status: published publisher: Impact Journals quality_controlled: '1' status: public title: Long term storage in liquid nitrogen leads to only minor phenotypic and gene expression changes in the mammary carcinoma model cell line BT474 type: journal_article user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87 volume: 8 year: '2017' ... --- _id: '627' abstract: - lang: eng text: Beige adipocytes are a new type of recruitable brownish adipocytes, with highly mitochondrial membrane uncoupling protein 1 expression and thermogenesis. Beige adipocytes were found among white adipocytes, especially in subcutaneous white adipose tissue (sWAT). Therefore, beige adipocytes may be involved in the regulation of energy metabolism and fat deposition. Transient receptor potential melastatin 8 (TRPM8), a Ca2+-permeable non-selective cation channel, plays vital roles in the regulation of various cellular functions. It has been reported that TRPM8 activation enhanced the thermogenic function of brown adiposytes. However, the involvement of TRPM8 in the thermogenic function of WAT remains unexplored. Our data revealed that TRPM8 was expressed in mouse white adipocytes at mRNA, protein and functional levels. The mRNA expression of Trpm8 was significantly increased in the differentiated white adipocytes than pre-adipocytes. Moreover, activation of TRPM8 by menthol enhanced the expression of thermogenic genes in cultured white aidpocytes. And menthol-induced increases of the thermogenic genes in white adipocytes was inhibited by either KT5720 (a protein kinase A inhibitor) or BAPTA-AM. In addition, high fat diet (HFD)-induced obesity in mice was significantly recovered by co-treatment with menthol. Dietary menthol enhanced WAT "browning" and improved glucose metabolism in HFD-induced obesity mice as well. Therefore, we concluded that TRPM8 might be involved in WAT "browning" by increasing the expression levels of genes related to thermogenesis and energy metabolism. And dietary menthol could be a novel approach for combating human obesity and related metabolic diseases. article_processing_charge: No author: - first_name: Changyu full_name: Jiang, Changyu last_name: Jiang - first_name: Ming-Zhu full_name: Zhai, Ming-Zhu id: 34009CFA-F248-11E8-B48F-1D18A9856A87 last_name: Zhai - first_name: Dong full_name: Yan, Dong last_name: Yan - first_name: Da full_name: Li, Da last_name: Li - first_name: Chen full_name: Li, Chen last_name: Li - first_name: Yonghong full_name: Zhang, Yonghong last_name: Zhang - first_name: Lizu full_name: Xiao, Lizu last_name: Xiao - first_name: Donglin full_name: Xiong, Donglin last_name: Xiong - first_name: Qiwen full_name: Deng, Qiwen last_name: Deng - first_name: Wuping full_name: Sun, Wuping last_name: Sun citation: ama: Jiang C, Zhai M-Z, Yan D, et al. Dietary menthol-induced TRPM8 activation enhances WAT “browning” and ameliorates diet-induced obesity. Oncotarget. 2017;8(43):75114-75126. doi:10.18632/oncotarget.20540 apa: Jiang, C., Zhai, M.-Z., Yan, D., Li, D., Li, C., Zhang, Y., … Sun, W. (2017). Dietary menthol-induced TRPM8 activation enhances WAT “browning” and ameliorates diet-induced obesity. Oncotarget. Impact Journals. https://doi.org/10.18632/oncotarget.20540 chicago: Jiang, Changyu, Ming-Zhu Zhai, Dong Yan, Da Li, Chen Li, Yonghong Zhang, Lizu Xiao, Donglin Xiong, Qiwen Deng, and Wuping Sun. “Dietary Menthol-Induced TRPM8 Activation Enhances WAT ‘Browning’ and Ameliorates Diet-Induced Obesity.” Oncotarget. Impact Journals, 2017. https://doi.org/10.18632/oncotarget.20540. ieee: C. Jiang et al., “Dietary menthol-induced TRPM8 activation enhances WAT ‘browning’ and ameliorates diet-induced obesity,” Oncotarget, vol. 8, no. 43. Impact Journals, pp. 75114–75126, 2017. ista: Jiang C, Zhai M-Z, Yan D, Li D, Li C, Zhang Y, Xiao L, Xiong D, Deng Q, Sun W. 2017. Dietary menthol-induced TRPM8 activation enhances WAT “browning” and ameliorates diet-induced obesity. Oncotarget. 8(43), 75114–75126. mla: Jiang, Changyu, et al. “Dietary Menthol-Induced TRPM8 Activation Enhances WAT ‘Browning’ and Ameliorates Diet-Induced Obesity.” Oncotarget, vol. 8, no. 43, Impact Journals, 2017, pp. 75114–26, doi:10.18632/oncotarget.20540. short: C. Jiang, M.-Z. Zhai, D. Yan, D. Li, C. Li, Y. Zhang, L. Xiao, D. Xiong, Q. Deng, W. Sun, Oncotarget 8 (2017) 75114–75126. date_created: 2018-12-11T11:47:34Z date_published: 2017-08-24T00:00:00Z date_updated: 2023-10-17T08:56:37Z day: '24' ddc: - '571' department: - _id: RySh doi: 10.18632/oncotarget.20540 file: - access_level: open_access checksum: 2219e5348bbfe1aac2725aa620c33280 content_type: application/pdf creator: system date_created: 2018-12-12T10:16:15Z date_updated: 2020-07-14T12:47:26Z file_id: '5201' file_name: IST-2017-907-v1+1_20540-294640-4-PB.pdf file_size: 6101606 relation: main_file file_date_updated: 2020-07-14T12:47:26Z has_accepted_license: '1' intvolume: ' 8' issue: '43' language: - iso: eng month: '08' oa: 1 oa_version: Published Version page: 75114 - 75126 publication: Oncotarget publication_identifier: issn: - 1949-2553 publication_status: published publisher: Impact Journals publist_id: '7167' pubrep_id: '907' quality_controlled: '1' scopus_import: '1' status: public title: Dietary menthol-induced TRPM8 activation enhances WAT “browning” and ameliorates diet-induced obesity tmp: image: /images/cc_by.png legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0) short: CC BY (4.0) type: journal_article user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87 volume: 8 year: '2017' ...