[{"language":[{"iso":"eng"}],"quality_controlled":"1","page":"83128-83141","article_type":"original","publisher":"Impact Journals","author":[{"id":"36432834-F248-11E8-B48F-1D18A9856A87","full_name":"Fazekas-Singer, Judit","orcid":"0000-0002-8777-3502","last_name":"Fazekas-Singer","first_name":"Judit"},{"first_name":"Neydher","last_name":"Berroterán-Infante","full_name":"Berroterán-Infante, Neydher"},{"first_name":"Christina","last_name":"Rami-Mark","full_name":"Rami-Mark, Christina"},{"first_name":"Monika","last_name":"Dumanic","full_name":"Dumanic, Monika"},{"full_name":"Matz, Miroslawa","first_name":"Miroslawa","last_name":"Matz"},{"last_name":"Willmann","first_name":"Michael","full_name":"Willmann, Michael"},{"full_name":"Andreae, Fritz","last_name":"Andreae","first_name":"Fritz"},{"last_name":"Singer","first_name":"Josef","full_name":"Singer, Josef"},{"first_name":"Wolfgang","last_name":"Wadsak","full_name":"Wadsak, Wolfgang"},{"full_name":"Mitterhauser, Markus","first_name":"Markus","last_name":"Mitterhauser"},{"first_name":"Erika","last_name":"Jensen-Jarolim","full_name":"Jensen-Jarolim, Erika"}],"publication":"Oncotarget","_id":"8235","intvolume":"         8","month":"09","title":"Development of a radiolabeled caninized anti-EGFR antibody for comparative oncology trials","article_processing_charge":"No","date_created":"2020-08-10T11:53:18Z","publication_status":"published","oa_version":"Published Version","status":"public","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","extern":"1","main_file_link":[{"open_access":"1","url":"https://doi.org/10.18632/oncotarget.20914"}],"volume":8,"type":"journal_article","date_published":"2017-09-15T00:00:00Z","citation":{"ieee":"J. Singer <i>et al.</i>, “Development of a radiolabeled caninized anti-EGFR antibody for comparative oncology trials,” <i>Oncotarget</i>, vol. 8. Impact Journals, pp. 83128–83141, 2017.","chicago":"Singer, Judit, Neydher Berroterán-Infante, Christina Rami-Mark, Monika Dumanic, Miroslawa Matz, Michael Willmann, Fritz Andreae, et al. “Development of a Radiolabeled Caninized Anti-EGFR Antibody for Comparative Oncology Trials.” <i>Oncotarget</i>. Impact Journals, 2017. <a href=\"https://doi.org/10.18632/oncotarget.20914\">https://doi.org/10.18632/oncotarget.20914</a>.","ama":"Singer J, Berroterán-Infante N, Rami-Mark C, et al. Development of a radiolabeled caninized anti-EGFR antibody for comparative oncology trials. <i>Oncotarget</i>. 2017;8:83128-83141. doi:<a href=\"https://doi.org/10.18632/oncotarget.20914\">10.18632/oncotarget.20914</a>","apa":"Singer, J., Berroterán-Infante, N., Rami-Mark, C., Dumanic, M., Matz, M., Willmann, M., … Jensen-Jarolim, E. (2017). Development of a radiolabeled caninized anti-EGFR antibody for comparative oncology trials. <i>Oncotarget</i>. Impact Journals. <a href=\"https://doi.org/10.18632/oncotarget.20914\">https://doi.org/10.18632/oncotarget.20914</a>","ista":"Singer J, Berroterán-Infante N, Rami-Mark C, Dumanic M, Matz M, Willmann M, Andreae F, Singer J, Wadsak W, Mitterhauser M, Jensen-Jarolim E. 2017. Development of a radiolabeled caninized anti-EGFR antibody for comparative oncology trials. Oncotarget. 8, 83128–83141.","short":"J. Singer, N. Berroterán-Infante, C. Rami-Mark, M. Dumanic, M. Matz, M. Willmann, F. Andreae, J. Singer, W. Wadsak, M. Mitterhauser, E. Jensen-Jarolim, Oncotarget 8 (2017) 83128–83141.","mla":"Singer, Judit, et al. “Development of a Radiolabeled Caninized Anti-EGFR Antibody for Comparative Oncology Trials.” <i>Oncotarget</i>, vol. 8, Impact Journals, 2017, pp. 83128–41, doi:<a href=\"https://doi.org/10.18632/oncotarget.20914\">10.18632/oncotarget.20914</a>."},"year":"2017","date_updated":"2021-01-12T08:17:39Z","oa":1,"abstract":[{"lang":"eng","text":"Due to large homology of human and canine EGFR, dogs suffering from spontaneous EGFR+ cancer can be considered as ideal translational models. Thereby, novel immunotherapeutic compounds can be developed for both human and veterinary patients. This study describes the radiolabeling of a canine anti-EGFR IgG antibody (can225IgG) with potential diagnostic and therapeutic value in comparative clinical settings. Can225IgG was functionalized with DTPA for subsequent chelation with the radionuclide 99mTc. Successful coupling of 10 DTPA molecules per antibody on average was proven by significant mass increase in MALDI-TOF spectroscopy, gel electrophoresis and immunoblots. Following functionalization and radiolabeling, 99mTc-DTPA-can225IgG fully retained its binding capacity towards human and canine EGFR in flow cytometry, immuno- and radioblots, and autoradiography. The affinity of radiolabeled can225IgG was determined to KD 0.8 ±0.0031 nM in a real-time kinetics assay on canine carcinoma cells by a competition binding technique. Stability tests of the radiolabeled compound identified TRIS buffered saline as the ideal formulation for short-term storage with 87.11 ±6.04% intact compound being still detected 60 minutes post radiolabeling. High stability, specificity and EGFR binding affinity pinpoint towards 99mTc-radiolabeled can225IgG antibody as an ideal lead compound for the first proof-of-concept diagnostic and therapeutic applications in canine cancer patients."}],"publication_identifier":{"issn":["1949-2553"]},"day":"15","doi":"10.18632/oncotarget.20914"},{"publisher":"Impact Journals","article_type":"original","page":"35076-35087","quality_controlled":"1","language":[{"iso":"eng"}],"publication_status":"published","oa_version":"Published Version","date_created":"2020-08-10T11:53:53Z","article_processing_charge":"No","month":"03","title":"Long term storage in liquid nitrogen leads to only minor phenotypic and gene expression changes in the mammary carcinoma model cell line BT474","intvolume":"         8","publication":"Oncotarget","_id":"8240","author":[{"last_name":"Fazekas","first_name":"Judit","full_name":"Fazekas, Judit","orcid":"0000-0002-8777-3502","id":"36432834-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Grunt, Thomas W.","last_name":"Grunt","first_name":"Thomas W."},{"last_name":"Jensen-Jarolim","first_name":"Erika","full_name":"Jensen-Jarolim, Erika"},{"full_name":"Singer, Josef","first_name":"Josef","last_name":"Singer"}],"volume":8,"main_file_link":[{"open_access":"1","url":"https://doi.org/10.18632/oncotarget.16623"}],"extern":"1","status":"public","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","doi":"10.18632/oncotarget.16623","publication_identifier":{"issn":["1949-2553"]},"day":"28","abstract":[{"text":"Background/Aim: Cancer cell lines are indispensible surrogate models in cancer research, as they can be used off-the-shelf, expanded to the desired extent, easily modified and exchanged between research groups for affirmation, reproduction or follow-up experiments.\r\nAs malignant cells are prone to genomic instability, phenotypical changes may occur after certain passages in culture. Thus, cell lines have to be regularly authenticated to ensure data quality. In between experiments these cell lines are often stored in liquid nitrogen for extended time periods.\r\nAlthough freezing of cells is a necessary evil, little research is performed on how long-term storage affects cancer cell lines. Therefore, this study investigated the effects of a 28-year long liquid nitrogen storage period on BT474 cells with regard to phenotypical changes, differences in cell-surface receptor expression as well as cytokine and gene expressional variations.\r\nMethods: Two batches of BT474 cells, one frozen in 1986, the other directly purchased from ATCC were investigated by light microscopy, cell growth analysis, flow cytometry and cytokine as well as whole-transcriptome expression profiling.\r\nResults: The cell lines were morphologically indifferent and showed similar growth rates and similar cell-surface receptor expression. Transcriptome analysis revealed significant differences in only 26 of 40,716 investigated RefSeq transcripts with 4 of them being up-regulated and 22 down-regulated.\r\nConclusion: This study demonstrates that even after very long periods of storage in liquid nitrogen, cancer cell lines display only minimal changes in their gene expression profiles. However, also such minor changes should be carefully assessed before continuation of experiments, especially if phenotypic alterations can be additionally observed.","lang":"eng"}],"oa":1,"date_updated":"2021-01-12T08:17:41Z","citation":{"short":"J. Singer, T.W. Grunt, E. Jensen-Jarolim, J. Singer, Oncotarget 8 (2017) 35076–35087.","mla":"Singer, Judit, et al. “Long Term Storage in Liquid Nitrogen Leads to Only Minor Phenotypic and Gene Expression Changes in the Mammary Carcinoma Model Cell Line BT474.” <i>Oncotarget</i>, vol. 8, Impact Journals, 2017, pp. 35076–87, doi:<a href=\"https://doi.org/10.18632/oncotarget.16623\">10.18632/oncotarget.16623</a>.","ista":"Singer J, Grunt TW, Jensen-Jarolim E, Singer J. 2017. Long term storage in liquid nitrogen leads to only minor phenotypic and gene expression changes in the mammary carcinoma model cell line BT474. Oncotarget. 8, 35076–35087.","apa":"Singer, J., Grunt, T. W., Jensen-Jarolim, E., &#38; Singer, J. (2017). Long term storage in liquid nitrogen leads to only minor phenotypic and gene expression changes in the mammary carcinoma model cell line BT474. <i>Oncotarget</i>. Impact Journals. <a href=\"https://doi.org/10.18632/oncotarget.16623\">https://doi.org/10.18632/oncotarget.16623</a>","ama":"Singer J, Grunt TW, Jensen-Jarolim E, Singer J. Long term storage in liquid nitrogen leads to only minor phenotypic and gene expression changes in the mammary carcinoma model cell line BT474. <i>Oncotarget</i>. 2017;8:35076-35087. doi:<a href=\"https://doi.org/10.18632/oncotarget.16623\">10.18632/oncotarget.16623</a>","ieee":"J. Singer, T. W. Grunt, E. Jensen-Jarolim, and J. Singer, “Long term storage in liquid nitrogen leads to only minor phenotypic and gene expression changes in the mammary carcinoma model cell line BT474,” <i>Oncotarget</i>, vol. 8. Impact Journals, pp. 35076–35087, 2017.","chicago":"Singer, Judit, Thomas W. Grunt, Erika Jensen-Jarolim, and Josef Singer. “Long Term Storage in Liquid Nitrogen Leads to Only Minor Phenotypic and Gene Expression Changes in the Mammary Carcinoma Model Cell Line BT474.” <i>Oncotarget</i>. Impact Journals, 2017. <a href=\"https://doi.org/10.18632/oncotarget.16623\">https://doi.org/10.18632/oncotarget.16623</a>."},"year":"2017","date_published":"2017-03-28T00:00:00Z","type":"journal_article"},{"quality_controlled":"1","page":"75114 - 75126","file_date_updated":"2020-07-14T12:47:26Z","publisher":"Impact Journals","scopus_import":"1","_id":"627","issue":"43","author":[{"full_name":"Jiang, Changyu","last_name":"Jiang","first_name":"Changyu"},{"last_name":"Zhai","first_name":"Ming-Zhu","full_name":"Zhai, Ming-Zhu","id":"34009CFA-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Yan","first_name":"Dong","full_name":"Yan, Dong"},{"last_name":"Li","first_name":"Da","full_name":"Li, Da"},{"full_name":"Li, Chen","last_name":"Li","first_name":"Chen"},{"last_name":"Zhang","first_name":"Yonghong","full_name":"Zhang, Yonghong"},{"full_name":"Xiao, Lizu","first_name":"Lizu","last_name":"Xiao"},{"full_name":"Xiong, Donglin","last_name":"Xiong","first_name":"Donglin"},{"full_name":"Deng, Qiwen","first_name":"Qiwen","last_name":"Deng"},{"full_name":"Sun, Wuping","first_name":"Wuping","last_name":"Sun"}],"department":[{"_id":"RySh"}],"article_processing_charge":"No","date_created":"2018-12-11T11:47:34Z","publication_status":"published","intvolume":"         8","pubrep_id":"907","title":"Dietary menthol-induced TRPM8 activation enhances WAT “browning” and ameliorates diet-induced obesity","volume":8,"ddc":["571"],"year":"2017","citation":{"ama":"Jiang C, Zhai M-Z, Yan D, et al. Dietary menthol-induced TRPM8 activation enhances WAT “browning” and ameliorates diet-induced obesity. <i>Oncotarget</i>. 2017;8(43):75114-75126. doi:<a href=\"https://doi.org/10.18632/oncotarget.20540\">10.18632/oncotarget.20540</a>","apa":"Jiang, C., Zhai, M.-Z., Yan, D., Li, D., Li, C., Zhang, Y., … Sun, W. (2017). Dietary menthol-induced TRPM8 activation enhances WAT “browning” and ameliorates diet-induced obesity. <i>Oncotarget</i>. Impact Journals. <a href=\"https://doi.org/10.18632/oncotarget.20540\">https://doi.org/10.18632/oncotarget.20540</a>","ieee":"C. Jiang <i>et al.</i>, “Dietary menthol-induced TRPM8 activation enhances WAT ‘browning’ and ameliorates diet-induced obesity,” <i>Oncotarget</i>, vol. 8, no. 43. Impact Journals, pp. 75114–75126, 2017.","chicago":"Jiang, Changyu, Ming-Zhu Zhai, Dong Yan, Da Li, Chen Li, Yonghong Zhang, Lizu Xiao, Donglin Xiong, Qiwen Deng, and Wuping Sun. “Dietary Menthol-Induced TRPM8 Activation Enhances WAT ‘Browning’ and Ameliorates Diet-Induced Obesity.” <i>Oncotarget</i>. Impact Journals, 2017. <a href=\"https://doi.org/10.18632/oncotarget.20540\">https://doi.org/10.18632/oncotarget.20540</a>.","short":"C. Jiang, M.-Z. Zhai, D. Yan, D. Li, C. Li, Y. Zhang, L. Xiao, D. Xiong, Q. Deng, W. Sun, Oncotarget 8 (2017) 75114–75126.","mla":"Jiang, Changyu, et al. “Dietary Menthol-Induced TRPM8 Activation Enhances WAT ‘Browning’ and Ameliorates Diet-Induced Obesity.” <i>Oncotarget</i>, vol. 8, no. 43, Impact Journals, 2017, pp. 75114–26, doi:<a href=\"https://doi.org/10.18632/oncotarget.20540\">10.18632/oncotarget.20540</a>.","ista":"Jiang C, Zhai M-Z, Yan D, Li D, Li C, Zhang Y, Xiao L, Xiong D, Deng Q, Sun W. 2017. Dietary menthol-induced TRPM8 activation enhances WAT “browning” and ameliorates diet-induced obesity. Oncotarget. 8(43), 75114–75126."},"date_updated":"2023-10-17T08:56:37Z","day":"24","doi":"10.18632/oncotarget.20540","abstract":[{"lang":"eng","text":"Beige adipocytes are a new type of recruitable brownish adipocytes, with highly mitochondrial membrane uncoupling protein 1 expression and thermogenesis. Beige adipocytes were found among white adipocytes, especially in subcutaneous white adipose tissue (sWAT). Therefore, beige adipocytes may be involved in the regulation of energy metabolism and fat deposition. Transient receptor potential melastatin 8 (TRPM8), a Ca2+-permeable non-selective cation channel, plays vital roles in the regulation of various cellular functions. It has been reported that TRPM8 activation enhanced the thermogenic function of brown adiposytes. However, the involvement of TRPM8 in the thermogenic function of WAT remains unexplored. Our data revealed that TRPM8 was expressed in mouse white adipocytes at mRNA, protein and functional levels. The mRNA expression of Trpm8 was significantly increased in the differentiated white adipocytes than pre-adipocytes. Moreover, activation of TRPM8 by menthol enhanced the expression of thermogenic genes in cultured white aidpocytes. And menthol-induced increases of the thermogenic genes in white adipocytes was inhibited by either KT5720 (a protein kinase A inhibitor) or BAPTA-AM. In addition, high fat diet (HFD)-induced obesity in mice was significantly recovered by co-treatment with menthol. Dietary menthol enhanced WAT &quot;browning&quot; and improved glucose metabolism in HFD-induced obesity mice as well. Therefore, we concluded that TRPM8 might be involved in WAT &quot;browning&quot; by increasing the expression levels of genes related to thermogenesis and energy metabolism. And dietary menthol could be a novel approach for combating human obesity and related metabolic diseases."}],"language":[{"iso":"eng"}],"has_accepted_license":"1","publication":"Oncotarget","oa_version":"Published Version","month":"08","file":[{"creator":"system","file_id":"5201","access_level":"open_access","relation":"main_file","content_type":"application/pdf","file_name":"IST-2017-907-v1+1_20540-294640-4-PB.pdf","date_updated":"2020-07-14T12:47:26Z","file_size":6101606,"checksum":"2219e5348bbfe1aac2725aa620c33280","date_created":"2018-12-12T10:16:15Z"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","status":"public","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"type":"journal_article","date_published":"2017-08-24T00:00:00Z","publication_identifier":{"issn":["1949-2553"]},"oa":1,"publist_id":"7167"}]