---
_id: '8443'
abstract:
- lang: eng
  text: Characterizing the structure of membrane proteins (MPs) generally requires
    extraction from their native environment, most commonly with detergents. Yet,
    the physicochemical properties of detergent micelles and lipid bilayers differ
    markedly and could alter the structural organization of MPs, albeit without general
    rules. Dodecylphosphocholine (DPC) is the most widely used detergent for MP structure
    determination by NMR, but the physiological relevance of several prominent structures
    has been questioned, though indirectly, by other biophysical techniques, e.g.,
    functional/thermostability assay (TSA) and molecular dynamics (MD) simulations.
    Here, we resolve unambiguously this controversy by probing the functional relevance
    of three different mitochondrial carriers (MCs) in DPC at the atomic level, using
    an exhaustive set of solution-NMR experiments, complemented by functional/TSA
    and MD data. Our results provide atomic-level insight into the structure, substrate
    interaction and dynamics of the detergent–membrane protein complexes and demonstrates
    cogently that, while high-resolution NMR signals can be obtained for MCs in DPC,
    they systematically correspond to nonfunctional states.
article_processing_charge: No
article_type: original
author:
- first_name: Vilius
  full_name: Kurauskas, Vilius
  last_name: Kurauskas
- first_name: Audrey
  full_name: Hessel, Audrey
  last_name: Hessel
- first_name: Peixiang
  full_name: Ma, Peixiang
  last_name: Ma
- first_name: Paola
  full_name: Lunetti, Paola
  last_name: Lunetti
- first_name: Katharina
  full_name: Weinhäupl, Katharina
  last_name: Weinhäupl
- first_name: Lionel
  full_name: Imbert, Lionel
  last_name: Imbert
- first_name: Bernhard
  full_name: Brutscher, Bernhard
  last_name: Brutscher
- first_name: Martin S.
  full_name: King, Martin S.
  last_name: King
- first_name: Rémy
  full_name: Sounier, Rémy
  last_name: Sounier
- first_name: Vincenza
  full_name: Dolce, Vincenza
  last_name: Dolce
- first_name: Edmund R. S.
  full_name: Kunji, Edmund R. S.
  last_name: Kunji
- first_name: Loredana
  full_name: Capobianco, Loredana
  last_name: Capobianco
- first_name: Christophe
  full_name: Chipot, Christophe
  last_name: Chipot
- first_name: François
  full_name: Dehez, François
  last_name: Dehez
- first_name: Beate
  full_name: Bersch, Beate
  last_name: Bersch
- first_name: Paul
  full_name: Schanda, Paul
  id: 7B541462-FAF6-11E9-A490-E8DFE5697425
  last_name: Schanda
  orcid: 0000-0002-9350-7606
citation:
  ama: 'Kurauskas V, Hessel A, Ma P, et al. How detergent impacts membrane proteins:
    Atomic-level views of mitochondrial carriers in dodecylphosphocholine. <i>The
    Journal of Physical Chemistry Letters</i>. 2018;9(5):933-938. doi:<a href="https://doi.org/10.1021/acs.jpclett.8b00269">10.1021/acs.jpclett.8b00269</a>'
  apa: 'Kurauskas, V., Hessel, A., Ma, P., Lunetti, P., Weinhäupl, K., Imbert, L.,
    … Schanda, P. (2018). How detergent impacts membrane proteins: Atomic-level views
    of mitochondrial carriers in dodecylphosphocholine. <i>The Journal of Physical
    Chemistry Letters</i>. American Chemical Society. <a href="https://doi.org/10.1021/acs.jpclett.8b00269">https://doi.org/10.1021/acs.jpclett.8b00269</a>'
  chicago: 'Kurauskas, Vilius, Audrey Hessel, Peixiang Ma, Paola Lunetti, Katharina
    Weinhäupl, Lionel Imbert, Bernhard Brutscher, et al. “How Detergent Impacts Membrane
    Proteins: Atomic-Level Views of Mitochondrial Carriers in Dodecylphosphocholine.”
    <i>The Journal of Physical Chemistry Letters</i>. American Chemical Society, 2018.
    <a href="https://doi.org/10.1021/acs.jpclett.8b00269">https://doi.org/10.1021/acs.jpclett.8b00269</a>.'
  ieee: 'V. Kurauskas <i>et al.</i>, “How detergent impacts membrane proteins: Atomic-level
    views of mitochondrial carriers in dodecylphosphocholine,” <i>The Journal of Physical
    Chemistry Letters</i>, vol. 9, no. 5. American Chemical Society, pp. 933–938,
    2018.'
  ista: 'Kurauskas V, Hessel A, Ma P, Lunetti P, Weinhäupl K, Imbert L, Brutscher
    B, King MS, Sounier R, Dolce V, Kunji ERS, Capobianco L, Chipot C, Dehez F, Bersch
    B, Schanda P. 2018. How detergent impacts membrane proteins: Atomic-level views
    of mitochondrial carriers in dodecylphosphocholine. The Journal of Physical Chemistry
    Letters. 9(5), 933–938.'
  mla: 'Kurauskas, Vilius, et al. “How Detergent Impacts Membrane Proteins: Atomic-Level
    Views of Mitochondrial Carriers in Dodecylphosphocholine.” <i>The Journal of Physical
    Chemistry Letters</i>, vol. 9, no. 5, American Chemical Society, 2018, pp. 933–38,
    doi:<a href="https://doi.org/10.1021/acs.jpclett.8b00269">10.1021/acs.jpclett.8b00269</a>.'
  short: V. Kurauskas, A. Hessel, P. Ma, P. Lunetti, K. Weinhäupl, L. Imbert, B. Brutscher,
    M.S. King, R. Sounier, V. Dolce, E.R.S. Kunji, L. Capobianco, C. Chipot, F. Dehez,
    B. Bersch, P. Schanda, The Journal of Physical Chemistry Letters 9 (2018) 933–938.
date_created: 2020-09-18T10:05:45Z
date_published: 2018-02-03T00:00:00Z
date_updated: 2021-01-12T08:19:18Z
day: '03'
doi: 10.1021/acs.jpclett.8b00269
extern: '1'
intvolume: '         9'
issue: '5'
keyword:
- General Materials Science
language:
- iso: eng
month: '02'
oa_version: None
page: 933-938
publication: The Journal of Physical Chemistry Letters
publication_identifier:
  issn:
  - 1948-7185
publication_status: published
publisher: American Chemical Society
quality_controlled: '1'
status: public
title: 'How detergent impacts membrane proteins: Atomic-level views of mitochondrial
  carriers in dodecylphosphocholine'
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 9
year: '2018'
...