---
_id: '9822'
abstract:
- lang: eng
  text: Attachment of adhesive molecules on cell culture surfaces to restrict cell
    adhesion to defined areas and shapes has been vital for the progress of in vitro
    research. In currently existing patterning methods, a combination of pattern properties
    such as stability, precision, specificity, high-throughput outcome, and spatiotemporal
    control is highly desirable but challenging to achieve. Here, we introduce a versatile
    and high-throughput covalent photoimmobilization technique, comprising a light-dose-dependent
    patterning step and a subsequent functionalization of the pattern via click chemistry.
    This two-step process is feasible on arbitrary surfaces and allows for generation
    of sustainable patterns and gradients. The method is validated in different biological
    systems by patterning adhesive ligands on cell-repellent surfaces, thereby constraining
    the growth and migration of cells to the designated areas. We then implement a
    sequential photopatterning approach by adding a second switchable patterning step,
    allowing for spatiotemporal control over two distinct surface patterns. As a proof
    of concept, we reconstruct the dynamics of the tip/stalk cell switch during angiogenesis.
    Our results show that the spatiotemporal control provided by our “sequential photopatterning”
    system is essential for mimicking dynamic biological processes and that our innovative
    approach has great potential for further applications in cell science.
acknowledgement: We would like to thank Charlott Leu for the production of our chromium
  wafers, Louise Ritter for her contribution of the IF stainings in Figure 4, Shokoufeh
  Teymouri for her help with the Bioinert coated slides, and finally Prof. Dr. Joachim
  Rädler for his valuable scientific guidance.
article_processing_charge: Yes (in subscription journal)
article_type: original
author:
- first_name: Themistoklis
  full_name: Zisis, Themistoklis
  last_name: Zisis
- first_name: Jan
  full_name: Schwarz, Jan
  id: 346C1EC6-F248-11E8-B48F-1D18A9856A87
  last_name: Schwarz
- first_name: Miriam
  full_name: Balles, Miriam
  last_name: Balles
- first_name: Maibritt
  full_name: Kretschmer, Maibritt
  last_name: Kretschmer
- first_name: Maria
  full_name: Nemethova, Maria
  id: 34E27F1C-F248-11E8-B48F-1D18A9856A87
  last_name: Nemethova
- first_name: Remy P
  full_name: Chait, Remy P
  id: 3464AE84-F248-11E8-B48F-1D18A9856A87
  last_name: Chait
  orcid: 0000-0003-0876-3187
- first_name: Robert
  full_name: Hauschild, Robert
  id: 4E01D6B4-F248-11E8-B48F-1D18A9856A87
  last_name: Hauschild
  orcid: 0000-0001-9843-3522
- first_name: Janina
  full_name: Lange, Janina
  last_name: Lange
- first_name: Calin C
  full_name: Guet, Calin C
  id: 47F8433E-F248-11E8-B48F-1D18A9856A87
  last_name: Guet
  orcid: 0000-0001-6220-2052
- first_name: Michael K
  full_name: Sixt, Michael K
  id: 41E9FBEA-F248-11E8-B48F-1D18A9856A87
  last_name: Sixt
  orcid: 0000-0002-4561-241X
- first_name: Stefan
  full_name: Zahler, Stefan
  last_name: Zahler
citation:
  ama: Zisis T, Schwarz J, Balles M, et al. Sequential and switchable patterning for
    studying cellular processes under spatiotemporal control. <i>ACS Applied Materials
    and Interfaces</i>. 2021;13(30):35545–35560. doi:<a href="https://doi.org/10.1021/acsami.1c09850">10.1021/acsami.1c09850</a>
  apa: Zisis, T., Schwarz, J., Balles, M., Kretschmer, M., Nemethova, M., Chait, R.
    P., … Zahler, S. (2021). Sequential and switchable patterning for studying cellular
    processes under spatiotemporal control. <i>ACS Applied Materials and Interfaces</i>.
    American Chemical Society. <a href="https://doi.org/10.1021/acsami.1c09850">https://doi.org/10.1021/acsami.1c09850</a>
  chicago: Zisis, Themistoklis, Jan Schwarz, Miriam Balles, Maibritt Kretschmer, Maria
    Nemethova, Remy P Chait, Robert Hauschild, et al. “Sequential and Switchable Patterning
    for Studying Cellular Processes under Spatiotemporal Control.” <i>ACS Applied
    Materials and Interfaces</i>. American Chemical Society, 2021. <a href="https://doi.org/10.1021/acsami.1c09850">https://doi.org/10.1021/acsami.1c09850</a>.
  ieee: T. Zisis <i>et al.</i>, “Sequential and switchable patterning for studying
    cellular processes under spatiotemporal control,” <i>ACS Applied Materials and
    Interfaces</i>, vol. 13, no. 30. American Chemical Society, pp. 35545–35560, 2021.
  ista: Zisis T, Schwarz J, Balles M, Kretschmer M, Nemethova M, Chait RP, Hauschild
    R, Lange J, Guet CC, Sixt MK, Zahler S. 2021. Sequential and switchable patterning
    for studying cellular processes under spatiotemporal control. ACS Applied Materials
    and Interfaces. 13(30), 35545–35560.
  mla: Zisis, Themistoklis, et al. “Sequential and Switchable Patterning for Studying
    Cellular Processes under Spatiotemporal Control.” <i>ACS Applied Materials and
    Interfaces</i>, vol. 13, no. 30, American Chemical Society, 2021, pp. 35545–35560,
    doi:<a href="https://doi.org/10.1021/acsami.1c09850">10.1021/acsami.1c09850</a>.
  short: T. Zisis, J. Schwarz, M. Balles, M. Kretschmer, M. Nemethova, R.P. Chait,
    R. Hauschild, J. Lange, C.C. Guet, M.K. Sixt, S. Zahler, ACS Applied Materials
    and Interfaces 13 (2021) 35545–35560.
date_created: 2021-08-08T22:01:28Z
date_published: 2021-08-04T00:00:00Z
date_updated: 2023-08-10T14:22:48Z
day: '04'
ddc:
- '620'
- '570'
department:
- _id: MiSi
- _id: GaTk
- _id: Bio
- _id: CaGu
doi: 10.1021/acsami.1c09850
ec_funded: 1
external_id:
  isi:
  - '000683741400026'
  pmid:
  - '34283577'
file:
- access_level: open_access
  checksum: b043a91d9f9200e467b970b692687ed3
  content_type: application/pdf
  creator: asandaue
  date_created: 2021-08-09T09:44:03Z
  date_updated: 2021-08-09T09:44:03Z
  file_id: '9833'
  file_name: 2021_ACSAppliedMaterialsAndInterfaces_Zisis.pdf
  file_size: 7123293
  relation: main_file
  success: 1
file_date_updated: 2021-08-09T09:44:03Z
has_accepted_license: '1'
intvolume: '        13'
isi: 1
issue: '30'
language:
- iso: eng
month: '08'
oa: 1
oa_version: Published Version
page: 35545–35560
pmid: 1
project:
- _id: 25FE9508-B435-11E9-9278-68D0E5697425
  call_identifier: H2020
  grant_number: '724373'
  name: Cellular navigation along spatial gradients
publication: ACS Applied Materials and Interfaces
publication_identifier:
  eissn:
  - '19448252'
  issn:
  - '19448244'
publication_status: published
publisher: American Chemical Society
quality_controlled: '1'
scopus_import: '1'
status: public
title: Sequential and switchable patterning for studying cellular processes under
  spatiotemporal control
tmp:
  image: /images/cc_by_nc_nd.png
  legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
  name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
    (CC BY-NC-ND 4.0)
  short: CC BY-NC-ND (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 13
year: '2021'
...
---
_id: '1020'
abstract:
- lang: eng
  text: Cellulose is the most abundant biopolymer on Earth. Cellulose fibers, such
    as the one extracted form cotton or woodpulp, have been used by humankind for
    hundreds of years to make textiles and paper. Here we show how, by engineering
    light-matter interaction, we can optimize light scattering using exclusively cellulose
    nanocrystals. The produced material is sustainable, biocompatible, and when compared
    to ordinary microfiber-based paper, it shows enhanced scattering strength (×4),
    yielding a transport mean free path as low as 3.5 μm in the visible light range.
    The experimental results are in a good agreement with the theoretical predictions
    obtained with a diffusive model for light propagation.
acknowledgement: This research was funded by the EPSRC (EP/M027961/1), the Leverhulme
  Trust (RPG-2014-238), Royal Society (RG140457), the BBSRC David Phillips fellowship
  (BB/K014617/1), and the European Research Council (ERC-2014-STG H2020 639088). All
  data created during this research are provided in full in the results section and
  Supporting Information. They are openly available from figshare and can be accessed
  at ref 30.
article_processing_charge: No
author:
- first_name: Soraya
  full_name: Caixeiro, Soraya
  last_name: Caixeiro
- first_name: Matilda
  full_name: Peruzzo, Matilda
  id: 3F920B30-F248-11E8-B48F-1D18A9856A87
  last_name: Peruzzo
  orcid: 0000-0002-3415-4628
- first_name: Olimpia
  full_name: Onelli, Olimpia
  last_name: Onelli
- first_name: Silvia
  full_name: Vignolini, Silvia
  last_name: Vignolini
- first_name: Riccardo
  full_name: Sapienza, Riccardo
  last_name: Sapienza
citation:
  ama: Caixeiro S, Peruzzo M, Onelli O, Vignolini S, Sapienza R. Disordered cellulose
    based nanostructures for enhanced light scattering. <i>ACS Applied Materials and
    Interfaces</i>. 2017;9(9):7885-7890. doi:<a href="https://doi.org/10.1021/acsami.6b15986">10.1021/acsami.6b15986</a>
  apa: Caixeiro, S., Peruzzo, M., Onelli, O., Vignolini, S., &#38; Sapienza, R. (2017).
    Disordered cellulose based nanostructures for enhanced light scattering. <i>ACS
    Applied Materials and Interfaces</i>. American Chemical Society. <a href="https://doi.org/10.1021/acsami.6b15986">https://doi.org/10.1021/acsami.6b15986</a>
  chicago: Caixeiro, Soraya, Matilda Peruzzo, Olimpia Onelli, Silvia Vignolini, and
    Riccardo Sapienza. “Disordered Cellulose Based Nanostructures for Enhanced Light
    Scattering.” <i>ACS Applied Materials and Interfaces</i>. American Chemical Society,
    2017. <a href="https://doi.org/10.1021/acsami.6b15986">https://doi.org/10.1021/acsami.6b15986</a>.
  ieee: S. Caixeiro, M. Peruzzo, O. Onelli, S. Vignolini, and R. Sapienza, “Disordered
    cellulose based nanostructures for enhanced light scattering,” <i>ACS Applied
    Materials and Interfaces</i>, vol. 9, no. 9. American Chemical Society, pp. 7885–7890,
    2017.
  ista: Caixeiro S, Peruzzo M, Onelli O, Vignolini S, Sapienza R. 2017. Disordered
    cellulose based nanostructures for enhanced light scattering. ACS Applied Materials
    and Interfaces. 9(9), 7885–7890.
  mla: Caixeiro, Soraya, et al. “Disordered Cellulose Based Nanostructures for Enhanced
    Light Scattering.” <i>ACS Applied Materials and Interfaces</i>, vol. 9, no. 9,
    American Chemical Society, 2017, pp. 7885–90, doi:<a href="https://doi.org/10.1021/acsami.6b15986">10.1021/acsami.6b15986</a>.
  short: S. Caixeiro, M. Peruzzo, O. Onelli, S. Vignolini, R. Sapienza, ACS Applied
    Materials and Interfaces 9 (2017) 7885–7890.
date_created: 2018-12-11T11:49:44Z
date_published: 2017-03-08T00:00:00Z
date_updated: 2023-09-22T09:40:14Z
day: '08'
department:
- _id: JoFi
doi: 10.1021/acsami.6b15986
external_id:
  isi:
  - '000396186000002'
intvolume: '         9'
isi: 1
issue: '9'
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: https://arxiv.org/abs/1702.01415
month: '03'
oa: 1
oa_version: Submitted Version
page: 7885 - 7890
publication: ACS Applied Materials and Interfaces
publication_identifier:
  issn:
  - '19448244'
publication_status: published
publisher: American Chemical Society
publist_id: '6372'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Disordered cellulose based nanostructures for enhanced light scattering
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 9
year: '2017'
...