@article{9822,
  abstract     = {Attachment of adhesive molecules on cell culture surfaces to restrict cell adhesion to defined areas and shapes has been vital for the progress of in vitro research. In currently existing patterning methods, a combination of pattern properties such as stability, precision, specificity, high-throughput outcome, and spatiotemporal control is highly desirable but challenging to achieve. Here, we introduce a versatile and high-throughput covalent photoimmobilization technique, comprising a light-dose-dependent patterning step and a subsequent functionalization of the pattern via click chemistry. This two-step process is feasible on arbitrary surfaces and allows for generation of sustainable patterns and gradients. The method is validated in different biological systems by patterning adhesive ligands on cell-repellent surfaces, thereby constraining the growth and migration of cells to the designated areas. We then implement a sequential photopatterning approach by adding a second switchable patterning step, allowing for spatiotemporal control over two distinct surface patterns. As a proof of concept, we reconstruct the dynamics of the tip/stalk cell switch during angiogenesis. Our results show that the spatiotemporal control provided by our “sequential photopatterning” system is essential for mimicking dynamic biological processes and that our innovative approach has great potential for further applications in cell science.},
  author       = {Zisis, Themistoklis and Schwarz, Jan and Balles, Miriam and Kretschmer, Maibritt and Nemethova, Maria and Chait, Remy P and Hauschild, Robert and Lange, Janina and Guet, Calin C and Sixt, Michael K and Zahler, Stefan},
  issn         = {19448252},
  journal      = {ACS Applied Materials and Interfaces},
  number       = {30},
  pages        = {35545–35560},
  publisher    = {American Chemical Society},
  title        = {{Sequential and switchable patterning for studying cellular processes under spatiotemporal control}},
  doi          = {10.1021/acsami.1c09850},
  volume       = {13},
  year         = {2021},
}

@article{1020,
  abstract     = {Cellulose is the most abundant biopolymer on Earth. Cellulose fibers, such as the one extracted form cotton or woodpulp, have been used by humankind for hundreds of years to make textiles and paper. Here we show how, by engineering light-matter interaction, we can optimize light scattering using exclusively cellulose nanocrystals. The produced material is sustainable, biocompatible, and when compared to ordinary microfiber-based paper, it shows enhanced scattering strength (×4), yielding a transport mean free path as low as 3.5 μm in the visible light range. The experimental results are in a good agreement with the theoretical predictions obtained with a diffusive model for light propagation.},
  author       = {Caixeiro, Soraya and Peruzzo, Matilda and Onelli, Olimpia and Vignolini, Silvia and Sapienza, Riccardo},
  issn         = {19448244},
  journal      = {ACS Applied Materials and Interfaces},
  number       = {9},
  pages        = {7885 -- 7890},
  publisher    = {American Chemical Society},
  title        = {{Disordered cellulose based nanostructures for enhanced light scattering}},
  doi          = {10.1021/acsami.6b15986},
  volume       = {9},
  year         = {2017},
}