---
_id: '8450'
abstract:
- lang: eng
  text: Methyl groups are very useful probes of structure, dynamics, and interactions
    in protein NMR spectroscopy. In particular, methyl-directed experiments provide
    high sensitivity even in very large proteins, such as membrane proteins in a membrane-mimicking
    environment. In this chapter, we discuss the approach for labeling methyl groups
    in E. coli-based protein expression, as exemplified with the mitochondrial carrier
    GGC.
alternative_title:
- Methods in Molecular Biology
article_processing_charge: No
author:
- first_name: Vilius
  full_name: Kurauskas, Vilius
  last_name: Kurauskas
- first_name: Paul
  full_name: Schanda, Paul
  id: 7B541462-FAF6-11E9-A490-E8DFE5697425
  last_name: Schanda
  orcid: 0000-0002-9350-7606
- first_name: Remy
  full_name: Sounier, Remy
  last_name: Sounier
citation:
  ama: 'Kurauskas V, Schanda P, Sounier R. Methyl-specific isotope labeling strategies
    for NMR studies of membrane proteins. In: <i>Membrane Protein Structure and Function
    Characterization</i>. Vol 1635. Springer Nature; 2017:109-123. doi:<a href="https://doi.org/10.1007/978-1-4939-7151-0_6">10.1007/978-1-4939-7151-0_6</a>'
  apa: Kurauskas, V., Schanda, P., &#38; Sounier, R. (2017). Methyl-specific isotope
    labeling strategies for NMR studies of membrane proteins. In <i>Membrane protein
    structure and function characterization</i> (Vol. 1635, pp. 109–123). Springer
    Nature. <a href="https://doi.org/10.1007/978-1-4939-7151-0_6">https://doi.org/10.1007/978-1-4939-7151-0_6</a>
  chicago: Kurauskas, Vilius, Paul Schanda, and Remy Sounier. “Methyl-Specific Isotope
    Labeling Strategies for NMR Studies of Membrane Proteins.” In <i>Membrane Protein
    Structure and Function Characterization</i>, 1635:109–23. Springer Nature, 2017.
    <a href="https://doi.org/10.1007/978-1-4939-7151-0_6">https://doi.org/10.1007/978-1-4939-7151-0_6</a>.
  ieee: V. Kurauskas, P. Schanda, and R. Sounier, “Methyl-specific isotope labeling
    strategies for NMR studies of membrane proteins,” in <i>Membrane protein structure
    and function characterization</i>, vol. 1635, Springer Nature, 2017, pp. 109–123.
  ista: 'Kurauskas V, Schanda P, Sounier R. 2017.Methyl-specific isotope labeling
    strategies for NMR studies of membrane proteins. In: Membrane protein structure
    and function characterization. Methods in Molecular Biology, vol. 1635, 109–123.'
  mla: Kurauskas, Vilius, et al. “Methyl-Specific Isotope Labeling Strategies for
    NMR Studies of Membrane Proteins.” <i>Membrane Protein Structure and Function
    Characterization</i>, vol. 1635, Springer Nature, 2017, pp. 109–23, doi:<a href="https://doi.org/10.1007/978-1-4939-7151-0_6">10.1007/978-1-4939-7151-0_6</a>.
  short: V. Kurauskas, P. Schanda, R. Sounier, in:, Membrane Protein Structure and
    Function Characterization, Springer Nature, 2017, pp. 109–123.
date_created: 2020-09-18T10:06:44Z
date_published: 2017-07-29T00:00:00Z
date_updated: 2022-08-26T09:14:20Z
day: '29'
doi: 10.1007/978-1-4939-7151-0_6
extern: '1'
intvolume: '      1635'
language:
- iso: eng
month: '07'
oa_version: None
page: 109-123
publication: Membrane protein structure and function characterization
publication_identifier:
  isbn:
  - '9781493971497'
  - '9781493971510'
  issn:
  - 1064-3745
  - 1940-6029
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
status: public
title: Methyl-specific isotope labeling strategies for NMR studies of membrane proteins
type: book_chapter
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
volume: 1635
year: '2017'
...