[{"volume":12,"extern":"1","doi":"10.1016/j.waojou.2019.100044","day":"29","abstract":[{"text":"Background: Atopics have a lower risk for malignancies, and IgE targeted to tumors is superior to IgG in fighting cancer. Whether IgE-mediated innate or adaptive immune surveillance can confer protection against tumors remains unclear.\r\nObjective: We aimed to investigate the effects of active and passive immunotherapy to the tumor-associated antigen HER-2 in three murine models differing in Epsilon-B-cell-receptor expression affecting the levels of expressed IgE.\r\nMethods: We compared the levels of several serum specific anti-HER-2 antibodies (IgE, IgG1, IgG2a, IgG2b, IgA) and the survival rates in low-IgE ΔM1M2 mice lacking the transmembrane/cytoplasmic domain of Epsilon-B-cell-receptors expressing reduced IgE levels, high-IgE KN1 mice expressing chimeric Epsilon-Gamma1-B-cell receptors with 4-6-fold elevated serum IgE levels, and wild type (WT) BALB/c. Prior engrafting mice with D2F2/E2 mammary tumors overexpressing HER-2, mice were vaccinated with HER-2 or vehicle control PBS using the Th2-adjuvant Al(OH)3 (active immunotherapy), or treated with the murine anti-HER-2 IgG1 antibody 4D5 (passive immunotherapy).\r\nResults: Overall, among the three strains of mice, HER-2 vaccination induced significantly higher levels of HER-2 specific IgE and IgG1 in high-IgE KN1, while low-IgE ΔM1M2 mice had higher IgG2a levels. HER-2 vaccination and passive immunotherapy prolonged the survival in tumor-grafted WT and low-IgE ΔM1M2 strains compared with treatment controls; active vaccination provided the highest benefit. Notably, untreated high-IgE KN1 mice displayed the longest survival of all strains, which could not be further extended by active or passive immunotherapy.\r\nConclusion: Active and passive immunotherapies prolong survival in wild type and low-IgE ΔM1M2 mice engrafted with mammary tumors. High-IgE KN1 mice have an innate survival benefit following tumor challenge.","lang":"eng"}],"date_updated":"2021-01-12T08:17:36Z","citation":{"chicago":"Singer, Josef, Gertrude Achatz-Straussberger, Anna Bentley-Lukschal, Judit Singer, Gernot Achatz, Sophia N. Karagiannis, and Erika Jensen-Jarolim. “AllergoOncology: High Innate IgE Levels Are Decisive for the Survival of Cancer-Bearing Mice.” <i>World Allergy Organization Journal</i>. Elsevier, 2019. <a href=\"https://doi.org/10.1016/j.waojou.2019.100044\">https://doi.org/10.1016/j.waojou.2019.100044</a>.","ieee":"J. Singer <i>et al.</i>, “AllergoOncology: High innate IgE levels are decisive for the survival of cancer-bearing mice,” <i>World Allergy Organization Journal</i>, vol. 12, no. 7. Elsevier, 2019.","ama":"Singer J, Achatz-Straussberger G, Bentley-Lukschal A, et al. AllergoOncology: High innate IgE levels are decisive for the survival of cancer-bearing mice. <i>World Allergy Organization Journal</i>. 2019;12(7). doi:<a href=\"https://doi.org/10.1016/j.waojou.2019.100044\">10.1016/j.waojou.2019.100044</a>","apa":"Singer, J., Achatz-Straussberger, G., Bentley-Lukschal, A., Singer, J., Achatz, G., Karagiannis, S. N., &#38; Jensen-Jarolim, E. (2019). AllergoOncology: High innate IgE levels are decisive for the survival of cancer-bearing mice. <i>World Allergy Organization Journal</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.waojou.2019.100044\">https://doi.org/10.1016/j.waojou.2019.100044</a>","ista":"Singer J, Achatz-Straussberger G, Bentley-Lukschal A, Singer J, Achatz G, Karagiannis SN, Jensen-Jarolim E. 2019. AllergoOncology: High innate IgE levels are decisive for the survival of cancer-bearing mice. World Allergy Organization Journal. 12(7), 100044.","short":"J. Singer, G. Achatz-Straussberger, A. Bentley-Lukschal, J. Singer, G. Achatz, S.N. Karagiannis, E. Jensen-Jarolim, World Allergy Organization Journal 12 (2019).","mla":"Singer, Josef, et al. “AllergoOncology: High Innate IgE Levels Are Decisive for the Survival of Cancer-Bearing Mice.” <i>World Allergy Organization Journal</i>, vol. 12, no. 7, 100044, Elsevier, 2019, doi:<a href=\"https://doi.org/10.1016/j.waojou.2019.100044\">10.1016/j.waojou.2019.100044</a>."},"year":"2019","publisher":"Elsevier","article_type":"original","quality_controlled":"1","publication_status":"published","article_processing_charge":"No","date_created":"2020-08-10T11:50:54Z","title":"AllergoOncology: High innate IgE levels are decisive for the survival of cancer-bearing mice","intvolume":"        12","_id":"8228","author":[{"orcid":"0000-0002-8701-2412","full_name":"Singer, Josef","first_name":"Josef","last_name":"Singer"},{"full_name":"Achatz-Straussberger, Gertrude","first_name":"Gertrude","last_name":"Achatz-Straussberger"},{"full_name":"Bentley-Lukschal, Anna","last_name":"Bentley-Lukschal","first_name":"Anna"},{"last_name":"Fazekas-Singer","first_name":"Judit","full_name":"Fazekas-Singer, Judit","orcid":"0000-0002-8777-3502","id":"36432834-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Achatz, Gernot","last_name":"Achatz","first_name":"Gernot"},{"full_name":"Karagiannis, Sophia N.","first_name":"Sophia N.","last_name":"Karagiannis"},{"full_name":"Jensen-Jarolim, Erika","first_name":"Erika","last_name":"Jensen-Jarolim"}],"issue":"7","main_file_link":[{"open_access":"1","url":"https://doi.org/10.1016/j.waojou.2019.100044"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","status":"public","publication_identifier":{"issn":["1939-4551"]},"oa":1,"date_published":"2019-07-29T00:00:00Z","type":"journal_article","language":[{"iso":"eng"}],"oa_version":"Published Version","month":"07","article_number":"100044","publication":"World Allergy Organization Journal"}]