---
_id: '7548'
abstract:
- lang: eng
text: Although the aggregation of the amyloid-β peptide (Aβ) into amyloid fibrils
is a well-established hallmark of Alzheimer’s disease, the complex mechanisms
linking this process to neurodegeneration are still incompletely understood. The
nematode worm C. elegans is a valuable model organism through which to study these
mechanisms because of its simple nervous system and its relatively short lifespan.
Standard Aβ-based C. elegans models of Alzheimer’s disease are designed to study
the toxic effects of the overexpression of Aβ in the muscle or nervous systems.
However, the wide variety of effects associated with the tissue-level overexpression
of Aβ makes it difficult to single out and study specific cellular mechanisms
related to the onset of Alzheimer’s disease. Here, to better understand how to
investigate the early events affecting neuronal signalling, we created a C. elegans
model expressing Aβ42, the 42-residue form of Aβ, from a single-copy gene insertion
in just one pair of glutamatergic sensory neurons, the BAG neurons. In behavioural
assays, we found that the Aβ42-expressing animals displayed a subtle modulation
of the response to CO2, compared to controls. Ca2+ imaging revealed that the BAG
neurons in young Aβ42-expressing nematodes were activated more strongly than in
control animals, and that neuronal activation remained intact until old age. Taken
together, our results suggest that Aβ42-expression in this very subtle model of
AD is sufficient to modulate the behavioural response but not strong enough to
generate significant neurotoxicity, suggesting that slightly more aggressive perturbations
will enable effectively studies of the links between the modulation of a physiological
response and its associated neurotoxicity.
article_number: e0217746
article_processing_charge: No
article_type: original
author:
- first_name: Tessa
full_name: Sinnige, Tessa
last_name: Sinnige
- first_name: Prashanth
full_name: Ciryam, Prashanth
last_name: Ciryam
- first_name: Samuel
full_name: Casford, Samuel
last_name: Casford
- first_name: Christopher M.
full_name: Dobson, Christopher M.
last_name: Dobson
- first_name: Mario
full_name: de Bono, Mario
id: 4E3FF80E-F248-11E8-B48F-1D18A9856A87
last_name: de Bono
orcid: 0000-0001-8347-0443
- first_name: Michele
full_name: Vendruscolo, Michele
last_name: Vendruscolo
citation:
ama: Sinnige T, Ciryam P, Casford S, Dobson CM, de Bono M, Vendruscolo M. Expression
of the amyloid-β peptide in a single pair of C. elegans sensory neurons modulates
the associated behavioural response. PLOS ONE. 2019;14(5). doi:10.1371/journal.pone.0217746
apa: Sinnige, T., Ciryam, P., Casford, S., Dobson, C. M., de Bono, M., & Vendruscolo,
M. (2019). Expression of the amyloid-β peptide in a single pair of C. elegans
sensory neurons modulates the associated behavioural response. PLOS ONE.
Public Library of Science. https://doi.org/10.1371/journal.pone.0217746
chicago: Sinnige, Tessa, Prashanth Ciryam, Samuel Casford, Christopher M. Dobson,
Mario de Bono, and Michele Vendruscolo. “Expression of the Amyloid-β Peptide in
a Single Pair of C. Elegans Sensory Neurons Modulates the Associated Behavioural
Response.” PLOS ONE. Public Library of Science, 2019. https://doi.org/10.1371/journal.pone.0217746.
ieee: T. Sinnige, P. Ciryam, S. Casford, C. M. Dobson, M. de Bono, and M. Vendruscolo,
“Expression of the amyloid-β peptide in a single pair of C. elegans sensory neurons
modulates the associated behavioural response,” PLOS ONE, vol. 14, no.
5. Public Library of Science, 2019.
ista: Sinnige T, Ciryam P, Casford S, Dobson CM, de Bono M, Vendruscolo M. 2019.
Expression of the amyloid-β peptide in a single pair of C. elegans sensory neurons
modulates the associated behavioural response. PLOS ONE. 14(5), e0217746.
mla: Sinnige, Tessa, et al. “Expression of the Amyloid-β Peptide in a Single Pair
of C. Elegans Sensory Neurons Modulates the Associated Behavioural Response.”
PLOS ONE, vol. 14, no. 5, e0217746, Public Library of Science, 2019, doi:10.1371/journal.pone.0217746.
short: T. Sinnige, P. Ciryam, S. Casford, C.M. Dobson, M. de Bono, M. Vendruscolo,
PLOS ONE 14 (2019).
date_created: 2020-02-28T10:45:13Z
date_published: 2019-05-31T00:00:00Z
date_updated: 2021-01-12T08:14:08Z
day: '31'
doi: 10.1371/journal.pone.0217746
extern: '1'
intvolume: ' 14'
issue: '5'
language:
- iso: eng
month: '05'
oa_version: Published Version
publication: PLOS ONE
publication_identifier:
issn:
- 1932-6203
publication_status: published
publisher: Public Library of Science
quality_controlled: '1'
status: public
title: Expression of the amyloid-β peptide in a single pair of C. elegans sensory
neurons modulates the associated behavioural response
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 14
year: '2019'
...
---
_id: '6142'
abstract:
- lang: eng
text: Defining the mutational landscape when individuals of a species grow separately
and diverge over many generations can provide insights into trait evolution. A
specific example of this involves studying changes associated with domestication
where different lines of the same wild stock have been cultivated independently
in different standard environments. Whole genome sequence comparison of such lines
permits estimation of mutation rates, inference of genes' ancestral states and
ancestry of existing strains, and correction of sequencing errors in genome databases.
Here we study domestication of the C. elegans Bristol strain as a model, and report
the genome sequence of LSJ1 (Bristol), a sibling of the standard C. elegans reference
wild type N2 (Bristol). The LSJ1 and N2 lines were cultivated separately from
shortly after the Bristol strain was isolated until methods to freeze C. elegans
were developed. We find that during this time the two strains have accumulated
1208 genetic differences. We describe phenotypic variation between N2 and LSJ1
in the rate at which embryos develop, the rate of production of eggs, the maturity
of eggs at laying, and feeding behavior, all the result of post-isolation changes.
We infer the ancestral alleles in the original Bristol isolate and highlight 2038
likely sequencing errors in the original N2 reference genome sequence. Many of
these changes modify genome annotation. Our study provides a starting point to
further investigate genotype-phenotype association and offers insights into the
process of selection as a result of laboratory domestication.
article_number: e13922
author:
- first_name: Katherine P.
full_name: Weber, Katherine P.
last_name: Weber
- first_name: Subhajyoti
full_name: De, Subhajyoti
last_name: De
- first_name: Iwanka
full_name: Kozarewa, Iwanka
last_name: Kozarewa
- first_name: Daniel J.
full_name: Turner, Daniel J.
last_name: Turner
- first_name: M. Madan
full_name: Babu, M. Madan
last_name: Babu
- first_name: Mario
full_name: de Bono, Mario
id: 4E3FF80E-F248-11E8-B48F-1D18A9856A87
last_name: de Bono
orcid: 0000-0001-8347-0443
citation:
ama: Weber KP, De S, Kozarewa I, Turner DJ, Babu MM, de Bono M. Whole genome sequencing
highlights genetic changes associated with laboratory domestication of C. elegans.
PLoS ONE. 2010;5(11). doi:10.1371/journal.pone.0013922
apa: Weber, K. P., De, S., Kozarewa, I., Turner, D. J., Babu, M. M., & de Bono,
M. (2010). Whole genome sequencing highlights genetic changes associated with
laboratory domestication of C. elegans. PLoS ONE. Public Library of Science.
https://doi.org/10.1371/journal.pone.0013922
chicago: Weber, Katherine P., Subhajyoti De, Iwanka Kozarewa, Daniel J. Turner,
M. Madan Babu, and Mario de Bono. “Whole Genome Sequencing Highlights Genetic
Changes Associated with Laboratory Domestication of C. Elegans.” PLoS ONE.
Public Library of Science, 2010. https://doi.org/10.1371/journal.pone.0013922.
ieee: K. P. Weber, S. De, I. Kozarewa, D. J. Turner, M. M. Babu, and M. de Bono,
“Whole genome sequencing highlights genetic changes associated with laboratory
domestication of C. elegans,” PLoS ONE, vol. 5, no. 11. Public Library
of Science, 2010.
ista: Weber KP, De S, Kozarewa I, Turner DJ, Babu MM, de Bono M. 2010. Whole genome
sequencing highlights genetic changes associated with laboratory domestication
of C. elegans. PLoS ONE. 5(11), e13922.
mla: Weber, Katherine P., et al. “Whole Genome Sequencing Highlights Genetic Changes
Associated with Laboratory Domestication of C. Elegans.” PLoS ONE, vol.
5, no. 11, e13922, Public Library of Science, 2010, doi:10.1371/journal.pone.0013922.
short: K.P. Weber, S. De, I. Kozarewa, D.J. Turner, M.M. Babu, M. de Bono, PLoS
ONE 5 (2010).
date_created: 2019-03-20T15:20:30Z
date_published: 2010-11-11T00:00:00Z
date_updated: 2021-01-12T08:06:20Z
day: '11'
ddc:
- '570'
doi: 10.1371/journal.pone.0013922
extern: '1'
external_id:
pmid:
- '21085631'
file:
- access_level: open_access
checksum: a01e6bbe15f044c0c79a26d7d881953e
content_type: application/pdf
creator: kschuh
date_created: 2019-03-20T15:22:48Z
date_updated: 2020-07-14T12:47:20Z
file_id: '6143'
file_name: 2010_PLOS_Weber.PDF
file_size: 578059
relation: main_file
file_date_updated: 2020-07-14T12:47:20Z
has_accepted_license: '1'
intvolume: ' 5'
issue: '11'
language:
- iso: eng
license: https://creativecommons.org/licenses/by/4.0/
month: '11'
oa: 1
oa_version: Published Version
pmid: 1
publication: PLoS ONE
publication_identifier:
issn:
- 1932-6203
publication_status: published
publisher: Public Library of Science
quality_controlled: '1'
status: public
title: Whole genome sequencing highlights genetic changes associated with laboratory
domestication of C. elegans
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
volume: 5
year: '2010'
...
---
_id: '11114'
abstract:
- lang: eng
text: We present a miniaturized pull-down method for the detection of protein-protein
interactions using standard affinity chromatography reagents. Binding events between
different proteins, which are color-coded with quantum dots (QDs), are visualized
on single affinity chromatography beads by fluorescence microscopy. The use of
QDs for single molecule detection allows the simultaneous analysis of multiple
protein-protein binding events and reduces the amount of time and material needed
to perform a pull-down experiment.
article_number: e2061
article_processing_charge: No
article_type: original
author:
- first_name: Roberta
full_name: Schulte, Roberta
last_name: Schulte
- first_name: Jessica
full_name: Talamas, Jessica
last_name: Talamas
- first_name: Christine
full_name: Doucet, Christine
last_name: Doucet
- first_name: Martin W
full_name: HETZER, Martin W
id: 86c0d31b-b4eb-11ec-ac5a-eae7b2e135ed
last_name: HETZER
orcid: 0000-0002-2111-992X
citation:
ama: Schulte R, Talamas J, Doucet C, Hetzer M. Single bead affinity detection (SINBAD)
for the analysis of protein-protein interactions. PLoS ONE. 2008;3(4).
doi:10.1371/journal.pone.0002061
apa: Schulte, R., Talamas, J., Doucet, C., & Hetzer, M. (2008). Single bead
affinity detection (SINBAD) for the analysis of protein-protein interactions.
PLoS ONE. Public Library of Science. https://doi.org/10.1371/journal.pone.0002061
chicago: Schulte, Roberta, Jessica Talamas, Christine Doucet, and Martin Hetzer.
“Single Bead Affinity Detection (SINBAD) for the Analysis of Protein-Protein Interactions.”
PLoS ONE. Public Library of Science, 2008. https://doi.org/10.1371/journal.pone.0002061.
ieee: R. Schulte, J. Talamas, C. Doucet, and M. Hetzer, “Single bead affinity detection
(SINBAD) for the analysis of protein-protein interactions,” PLoS ONE, vol.
3, no. 4. Public Library of Science, 2008.
ista: Schulte R, Talamas J, Doucet C, Hetzer M. 2008. Single bead affinity detection
(SINBAD) for the analysis of protein-protein interactions. PLoS ONE. 3(4), e2061.
mla: Schulte, Roberta, et al. “Single Bead Affinity Detection (SINBAD) for the Analysis
of Protein-Protein Interactions.” PLoS ONE, vol. 3, no. 4, e2061, Public
Library of Science, 2008, doi:10.1371/journal.pone.0002061.
short: R. Schulte, J. Talamas, C. Doucet, M. Hetzer, PLoS ONE 3 (2008).
date_created: 2022-04-07T07:55:57Z
date_published: 2008-04-30T00:00:00Z
date_updated: 2022-07-18T08:56:36Z
day: '30'
doi: 10.1371/journal.pone.0002061
extern: '1'
external_id:
pmid:
- '18446240'
intvolume: ' 3'
issue: '4'
keyword:
- Multidisciplinary
language:
- iso: eng
main_file_link:
- open_access: '1'
url: ' https://doi.org/10.1371/journal.pone.0002061'
month: '04'
oa: 1
oa_version: Published Version
pmid: 1
publication: PLoS ONE
publication_identifier:
issn:
- 1932-6203
publication_status: published
publisher: Public Library of Science
quality_controlled: '1'
scopus_import: '1'
status: public
title: Single bead affinity detection (SINBAD) for the analysis of protein-protein
interactions
type: journal_article
user_id: 72615eeb-f1f3-11ec-aa25-d4573ddc34fd
volume: 3
year: '2008'
...