@article{7548,
  abstract     = {Although the aggregation of the amyloid-β peptide (Aβ) into amyloid fibrils is a well-established hallmark of Alzheimer’s disease, the complex mechanisms linking this process to neurodegeneration are still incompletely understood. The nematode worm C. elegans is a valuable model organism through which to study these mechanisms because of its simple nervous system and its relatively short lifespan. Standard Aβ-based C. elegans models of Alzheimer’s disease are designed to study the toxic effects of the overexpression of Aβ in the muscle or nervous systems. However, the wide variety of effects associated with the tissue-level overexpression of Aβ makes it difficult to single out and study specific cellular mechanisms related to the onset of Alzheimer’s disease. Here, to better understand how to investigate the early events affecting neuronal signalling, we created a C. elegans model expressing Aβ42, the 42-residue form of Aβ, from a single-copy gene insertion in just one pair of glutamatergic sensory neurons, the BAG neurons. In behavioural assays, we found that the Aβ42-expressing animals displayed a subtle modulation of the response to CO2, compared to controls. Ca2+ imaging revealed that the BAG neurons in young Aβ42-expressing nematodes were activated more strongly than in control animals, and that neuronal activation remained intact until old age. Taken together, our results suggest that Aβ42-expression in this very subtle model of AD is sufficient to modulate the behavioural response but not strong enough to generate significant neurotoxicity, suggesting that slightly more aggressive perturbations will enable effectively studies of the links between the modulation of a physiological response and its associated neurotoxicity.},
  author       = {Sinnige, Tessa and Ciryam, Prashanth and Casford, Samuel and Dobson, Christopher M. and de Bono, Mario and Vendruscolo, Michele},
  issn         = {1932-6203},
  journal      = {PLOS ONE},
  number       = {5},
  publisher    = {Public Library of Science},
  title        = {{Expression of the amyloid-β peptide in a single pair of C. elegans sensory neurons modulates the associated behavioural response}},
  doi          = {10.1371/journal.pone.0217746},
  volume       = {14},
  year         = {2019},
}

@article{6142,
  abstract     = {Defining the mutational landscape when individuals of a species grow separately and diverge over many generations can provide insights into trait evolution. A specific example of this involves studying changes associated with domestication where different lines of the same wild stock have been cultivated independently in different standard environments. Whole genome sequence comparison of such lines permits estimation of mutation rates, inference of genes' ancestral states and ancestry of existing strains, and correction of sequencing errors in genome databases. Here we study domestication of the C. elegans Bristol strain as a model, and report the genome sequence of LSJ1 (Bristol), a sibling of the standard C. elegans reference wild type N2 (Bristol). The LSJ1 and N2 lines were cultivated separately from shortly after the Bristol strain was isolated until methods to freeze C. elegans were developed. We find that during this time the two strains have accumulated 1208 genetic differences. We describe phenotypic variation between N2 and LSJ1 in the rate at which embryos develop, the rate of production of eggs, the maturity of eggs at laying, and feeding behavior, all the result of post-isolation changes. We infer the ancestral alleles in the original Bristol isolate and highlight 2038 likely sequencing errors in the original N2 reference genome sequence. Many of these changes modify genome annotation. Our study provides a starting point to further investigate genotype-phenotype association and offers insights into the process of selection as a result of laboratory domestication.},
  author       = {Weber, Katherine P. and De, Subhajyoti and Kozarewa, Iwanka and Turner, Daniel J. and Babu, M. Madan and de Bono, Mario},
  issn         = {1932-6203},
  journal      = {PLoS ONE},
  number       = {11},
  publisher    = {Public Library of Science},
  title        = {{Whole genome sequencing highlights genetic changes associated with laboratory domestication of C. elegans}},
  doi          = {10.1371/journal.pone.0013922},
  volume       = {5},
  year         = {2010},
}

@article{11114,
  abstract     = {We present a miniaturized pull-down method for the detection of protein-protein interactions using standard affinity chromatography reagents. Binding events between different proteins, which are color-coded with quantum dots (QDs), are visualized on single affinity chromatography beads by fluorescence microscopy. The use of QDs for single molecule detection allows the simultaneous analysis of multiple protein-protein binding events and reduces the amount of time and material needed to perform a pull-down experiment.},
  author       = {Schulte, Roberta and Talamas, Jessica and Doucet, Christine and HETZER, Martin W},
  issn         = {1932-6203},
  journal      = {PLoS ONE},
  keywords     = {Multidisciplinary},
  number       = {4},
  publisher    = {Public Library of Science},
  title        = {{Single bead affinity detection (SINBAD) for the analysis of protein-protein interactions}},
  doi          = {10.1371/journal.pone.0002061},
  volume       = {3},
  year         = {2008},
}