[{"intvolume":"        15","scopus_import":"1","has_accepted_license":"1","isi":1,"title":"Three-dimensional visualization of planta clathrin-coated vesicles at ultrastructural resolution","oa":1,"date_updated":"2023-08-04T09:39:24Z","issue":"10","article_processing_charge":"Yes (via OA deal)","publication":"Molecular Plant","language":[{"iso":"eng"}],"oa_version":"Published Version","day":"03","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","file":[{"date_updated":"2023-01-30T07:46:51Z","date_created":"2023-01-30T07:46:51Z","file_id":"12435","checksum":"04d5c12490052d03e4dc4412338a43dd","file_name":"2022_MolecularPlant_Johnson.pdf","success":1,"relation":"main_file","content_type":"application/pdf","access_level":"open_access","file_size":2307251,"creator":"dernst"}],"month":"10","publication_identifier":{"issn":["1674-2052"]},"author":[{"orcid":"0000-0002-2739-8843","id":"46A62C3A-F248-11E8-B48F-1D18A9856A87","last_name":"Johnson","first_name":"Alexander J","full_name":"Johnson, Alexander J"},{"full_name":"Kaufmann, Walter","first_name":"Walter","last_name":"Kaufmann","id":"3F99E422-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-9735-5315"},{"orcid":"0000-0003-1216-9105","id":"4DF26D8C-F248-11E8-B48F-1D18A9856A87","last_name":"Sommer","full_name":"Sommer, Christoph M","first_name":"Christoph M"},{"id":"D93824F4-D9BA-11E9-BB12-F207E6697425","last_name":"Costanzo","orcid":"0000-0001-9732-3815","full_name":"Costanzo, Tommaso","first_name":"Tommaso"},{"last_name":"Dahhan","full_name":"Dahhan, Dana A.","first_name":"Dana A."},{"first_name":"Sebastian Y.","full_name":"Bednarek, Sebastian Y.","last_name":"Bednarek"},{"full_name":"Friml, Jiří","first_name":"Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml","orcid":"0000-0002-8302-7596"}],"pmid":1,"type":"journal_article","status":"public","date_created":"2023-01-16T09:51:49Z","citation":{"ista":"Johnson AJ, Kaufmann W, Sommer CM, Costanzo T, Dahhan DA, Bednarek SY, Friml J. 2022. Three-dimensional visualization of planta clathrin-coated vesicles at ultrastructural resolution. Molecular Plant. 15(10), 1533–1542.","chicago":"Johnson, Alexander J, Walter Kaufmann, Christoph M Sommer, Tommaso Costanzo, Dana A. Dahhan, Sebastian Y. Bednarek, and Jiří Friml. “Three-Dimensional Visualization of Planta Clathrin-Coated Vesicles at Ultrastructural Resolution.” <i>Molecular Plant</i>. Elsevier, 2022. <a href=\"https://doi.org/10.1016/j.molp.2022.09.003\">https://doi.org/10.1016/j.molp.2022.09.003</a>.","mla":"Johnson, Alexander J., et al. “Three-Dimensional Visualization of Planta Clathrin-Coated Vesicles at Ultrastructural Resolution.” <i>Molecular Plant</i>, vol. 15, no. 10, Elsevier, 2022, pp. 1533–42, doi:<a href=\"https://doi.org/10.1016/j.molp.2022.09.003\">10.1016/j.molp.2022.09.003</a>.","short":"A.J. Johnson, W. Kaufmann, C.M. Sommer, T. Costanzo, D.A. Dahhan, S.Y. Bednarek, J. Friml, Molecular Plant 15 (2022) 1533–1542.","ama":"Johnson AJ, Kaufmann W, Sommer CM, et al. Three-dimensional visualization of planta clathrin-coated vesicles at ultrastructural resolution. <i>Molecular Plant</i>. 2022;15(10):1533-1542. doi:<a href=\"https://doi.org/10.1016/j.molp.2022.09.003\">10.1016/j.molp.2022.09.003</a>","ieee":"A. J. Johnson <i>et al.</i>, “Three-dimensional visualization of planta clathrin-coated vesicles at ultrastructural resolution,” <i>Molecular Plant</i>, vol. 15, no. 10. Elsevier, pp. 1533–1542, 2022.","apa":"Johnson, A. J., Kaufmann, W., Sommer, C. M., Costanzo, T., Dahhan, D. A., Bednarek, S. Y., &#38; Friml, J. (2022). Three-dimensional visualization of planta clathrin-coated vesicles at ultrastructural resolution. <i>Molecular Plant</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.molp.2022.09.003\">https://doi.org/10.1016/j.molp.2022.09.003</a>"},"keyword":["Plant Science","Molecular Biology"],"acknowledgement":"A.J. is supported by funding from the Austrian Science Fund I3630B25 (to J.F.). This research was supported by the Scientific Service Units of Institute of Science and Technology Austria (ISTA) through resources provided by the Electron Microscopy Facility, Lab Support Facility, and the Imaging and Optics Facility. We acknowledge Prof. David Robinson (Heidelberg) and Prof. Jan Traas (Lyon) for making us aware of previously published classical on-grid preparation methods. No conflict of interest declared.","year":"2022","doi":"10.1016/j.molp.2022.09.003","acknowledged_ssus":[{"_id":"EM-Fac"},{"_id":"LifeSc"},{"_id":"Bio"}],"file_date_updated":"2023-01-30T07:46:51Z","article_type":"original","_id":"12239","external_id":{"isi":["000882769800009"],"pmid":["36081349"]},"abstract":[{"lang":"eng","text":"Biological systems are the sum of their dynamic three-dimensional (3D) parts. Therefore, it is critical to study biological structures in 3D and at high resolution to gain insights into their physiological functions. Electron microscopy of metal replicas of unroofed cells and isolated organelles has been a key technique to visualize intracellular structures at nanometer resolution. However, many of these methods require specialized equipment and personnel to complete them. Here, we present novel accessible methods to analyze biological structures in unroofed cells and biochemically isolated organelles in 3D and at nanometer resolution, focusing on Arabidopsis clathrin-coated vesicles (CCVs). While CCVs are essential trafficking organelles, their detailed structural information is lacking due to their poor preservation when observed via classical electron microscopy protocols experiments. First, we establish a method to visualize CCVs in unroofed cells using scanning transmission electron microscopy tomography, providing sufficient resolution to define the clathrin coat arrangements. Critically, the samples are prepared directly on electron microscopy grids, removing the requirement to use extremely corrosive acids, thereby enabling the use of this method in any electron microscopy lab. Secondly, we demonstrate that this standardized sample preparation allows the direct comparison of isolated CCV samples with those visualized in cells. Finally, to facilitate the high-throughput and robust screening of metal replicated samples, we provide a deep learning analysis method to screen the “pseudo 3D” morphologies of CCVs imaged with 2D modalities. Collectively, our work establishes accessible ways to examine the 3D structure of biological samples and provide novel insights into the structure of plant CCVs."}],"ddc":["580"],"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"publication_status":"published","page":"1533-1542","department":[{"_id":"JiFr"},{"_id":"EM-Fac"},{"_id":"Bio"}],"quality_controlled":"1","volume":15,"publisher":"Elsevier","date_published":"2022-10-03T00:00:00Z","project":[{"grant_number":"I03630","_id":"26538374-B435-11E9-9278-68D0E5697425","call_identifier":"FWF","name":"Molecular mechanisms of endocytic cargo recognition in plants"}]},{"publication_status":"published","external_id":{"pmid":["32087370"]},"abstract":[{"lang":"eng","text":"Protein abundance and localization at the plasma membrane (PM) shapes plant development and mediates adaptation to changing environmental conditions. It is regulated by ubiquitination, a post-translational modification crucial for the proper sorting of endocytosed PM proteins to the vacuole for subsequent degradation. To understand the significance and the variety of roles played by this reversible modification, the function of ubiquitin receptors, which translate the ubiquitin signature into a cellular response, needs to be elucidated. In this study, we show that TOL (TOM1-like) proteins function in plants as multivalent ubiquitin receptors, governing ubiquitinated cargo delivery to the vacuole via the conserved Endosomal Sorting Complex Required for Transport (ESCRT) pathway. TOL2 and TOL6 interact with components of the ESCRT machinery and bind to K63-linked ubiquitin via two tandemly arranged conserved ubiquitin-binding domains. Mutation of these domains results not only in a loss of ubiquitin binding but also altered localization, abolishing TOL6 ubiquitin receptor activity. Function and localization of TOL6 is itself regulated by ubiquitination, whereby TOL6 ubiquitination potentially modulates degradation of PM-localized cargoes, assisting in the fine-tuning of the delicate interplay between protein recycling and downregulation. Taken together, our findings demonstrate the function and regulation of a ubiquitin receptor that mediates vacuolar degradation of PM proteins in higher plants."}],"ddc":["580"],"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"page":"717-731","department":[{"_id":"EvBe"}],"volume":13,"quality_controlled":"1","publisher":"Elsevier","date_published":"2020-05-04T00:00:00Z","date_created":"2024-02-28T08:55:56Z","citation":{"short":"J. Moulinier-Anzola, M. Schwihla, L. De-Araújo, C. Artner, L. Jörg, N. Konstantinova, C. Luschnig, B. Korbei, Molecular Plant 13 (2020) 717–731.","ama":"Moulinier-Anzola J, Schwihla M, De-Araújo L, et al. TOLs function as ubiquitin receptors in the early steps of the ESCRT pathway in higher plants. <i>Molecular Plant</i>. 2020;13(5):717-731. doi:<a href=\"https://doi.org/10.1016/j.molp.2020.02.012\">10.1016/j.molp.2020.02.012</a>","ieee":"J. Moulinier-Anzola <i>et al.</i>, “TOLs function as ubiquitin receptors in the early steps of the ESCRT pathway in higher plants,” <i>Molecular Plant</i>, vol. 13, no. 5. Elsevier, pp. 717–731, 2020.","ista":"Moulinier-Anzola J, Schwihla M, De-Araújo L, Artner C, Jörg L, Konstantinova N, Luschnig C, Korbei B. 2020. TOLs function as ubiquitin receptors in the early steps of the ESCRT pathway in higher plants. Molecular Plant. 13(5), 717–731.","mla":"Moulinier-Anzola, Jeanette, et al. “TOLs Function as Ubiquitin Receptors in the Early Steps of the ESCRT Pathway in Higher Plants.” <i>Molecular Plant</i>, vol. 13, no. 5, Elsevier, 2020, pp. 717–31, doi:<a href=\"https://doi.org/10.1016/j.molp.2020.02.012\">10.1016/j.molp.2020.02.012</a>.","chicago":"Moulinier-Anzola, Jeanette, Maximilian Schwihla, Lucinda De-Araújo, Christina Artner, Lisa Jörg, Nataliia Konstantinova, Christian Luschnig, and Barbara Korbei. “TOLs Function as Ubiquitin Receptors in the Early Steps of the ESCRT Pathway in Higher Plants.” <i>Molecular Plant</i>. Elsevier, 2020. <a href=\"https://doi.org/10.1016/j.molp.2020.02.012\">https://doi.org/10.1016/j.molp.2020.02.012</a>.","apa":"Moulinier-Anzola, J., Schwihla, M., De-Araújo, L., Artner, C., Jörg, L., Konstantinova, N., … Korbei, B. (2020). TOLs function as ubiquitin receptors in the early steps of the ESCRT pathway in higher plants. <i>Molecular Plant</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.molp.2020.02.012\">https://doi.org/10.1016/j.molp.2020.02.012</a>"},"keyword":["Plant Science","Molecular Biology"],"year":"2020","file_date_updated":"2024-02-28T12:39:56Z","_id":"15037","article_type":"original","doi":"10.1016/j.molp.2020.02.012","author":[{"first_name":"Jeanette","full_name":"Moulinier-Anzola, Jeanette","last_name":"Moulinier-Anzola"},{"full_name":"Schwihla, Maximilian","first_name":"Maximilian","last_name":"Schwihla"},{"first_name":"Lucinda","full_name":"De-Araújo, Lucinda","last_name":"De-Araújo"},{"last_name":"Artner","id":"45DF286A-F248-11E8-B48F-1D18A9856A87","first_name":"Christina","full_name":"Artner, Christina"},{"first_name":"Lisa","full_name":"Jörg, Lisa","last_name":"Jörg"},{"first_name":"Nataliia","full_name":"Konstantinova, Nataliia","last_name":"Konstantinova"},{"full_name":"Luschnig, Christian","first_name":"Christian","last_name":"Luschnig"},{"last_name":"Korbei","full_name":"Korbei, Barbara","first_name":"Barbara"}],"oa_version":"Published Version","day":"04","file":[{"creator":"dernst","content_type":"application/pdf","file_size":3089212,"access_level":"open_access","relation":"main_file","file_id":"15038","file_name":"2020_MolecularPlant_MoulinierAnzola.pdf","checksum":"c538a5008f7827f62d17d40a3bfabe65","success":1,"date_updated":"2024-02-28T12:39:56Z","date_created":"2024-02-28T12:39:56Z"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","publication_identifier":{"issn":["1674-2052"]},"month":"05","pmid":1,"status":"public","type":"journal_article","title":"TOLs function as ubiquitin receptors in the early steps of the ESCRT pathway in higher plants","oa":1,"date_updated":"2024-02-28T12:41:52Z","issue":"5","article_processing_charge":"No","has_accepted_license":"1","intvolume":"        13","language":[{"iso":"eng"}],"publication":"Molecular Plant"},{"doi":"10.1016/j.molp.2019.09.003","_id":"6920","article_type":"original","year":"2019","citation":{"apa":"Artner, C., &#38; Benková, E. (2019). Ethylene and cytokinin - partners in root growth regulation. <i>Molecular Plant</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.molp.2019.09.003\">https://doi.org/10.1016/j.molp.2019.09.003</a>","ista":"Artner C, Benková E. 2019. Ethylene and cytokinin - partners in root growth regulation. Molecular Plant. 12(10), 1312–1314.","mla":"Artner, Christina, and Eva Benková. “Ethylene and Cytokinin - Partners in Root Growth Regulation.” <i>Molecular Plant</i>, vol. 12, no. 10, Cell Press, 2019, pp. 1312–14, doi:<a href=\"https://doi.org/10.1016/j.molp.2019.09.003\">10.1016/j.molp.2019.09.003</a>.","chicago":"Artner, Christina, and Eva Benková. “Ethylene and Cytokinin - Partners in Root Growth Regulation.” <i>Molecular Plant</i>. Cell Press, 2019. <a href=\"https://doi.org/10.1016/j.molp.2019.09.003\">https://doi.org/10.1016/j.molp.2019.09.003</a>.","short":"C. Artner, E. Benková, Molecular Plant 12 (2019) 1312–1314.","ama":"Artner C, Benková E. Ethylene and cytokinin - partners in root growth regulation. <i>Molecular Plant</i>. 2019;12(10):1312-1314. doi:<a href=\"https://doi.org/10.1016/j.molp.2019.09.003\">10.1016/j.molp.2019.09.003</a>","ieee":"C. Artner and E. Benková, “Ethylene and cytokinin - partners in root growth regulation,” <i>Molecular Plant</i>, vol. 12, no. 10. Cell Press, pp. 1312–1314, 2019."},"date_created":"2019-09-30T10:00:40Z","date_published":"2019-10-07T00:00:00Z","publisher":"Cell Press","volume":12,"quality_controlled":"1","page":"1312-1314","department":[{"_id":"EvBe"}],"project":[{"name":"Hormonal regulation of plant adaptive responses to environmental signals","_id":"2685A872-B435-11E9-9278-68D0E5697425"}],"external_id":{"isi":["000489132500002"],"pmid":["31541740"]},"publication_status":"published","publication":"Molecular Plant","language":[{"iso":"eng"}],"isi":1,"intvolume":"        12","scopus_import":"1","article_processing_charge":"No","issue":"10","date_updated":"2023-08-30T06:55:02Z","title":"Ethylene and cytokinin - partners in root growth regulation","status":"public","type":"journal_article","pmid":1,"publication_identifier":{"issn":["1674-2052","1752-9867"]},"month":"10","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","oa_version":"None","day":"07","author":[{"first_name":"Christina","full_name":"Artner, Christina","id":"45DF286A-F248-11E8-B48F-1D18A9856A87","last_name":"Artner"},{"first_name":"Eva","full_name":"Benková, Eva","orcid":"0000-0002-8510-9739","last_name":"Benková","id":"38F4F166-F248-11E8-B48F-1D18A9856A87"}]}]