---
_id: '7665'
abstract:
- lang: eng
  text: Acute brain slice preparation is a powerful experimental model for investigating
    the characteristics of synaptic function in the brain. Although brain tissue is
    usually cut at ice-cold temperature (CT) to facilitate slicing and avoid neuronal
    damage, exposure to CT causes molecular and architectural changes of synapses.
    To address these issues, we investigated ultrastructural and electrophysiological
    features of synapses in mouse acute cerebellar slices prepared at ice-cold and
    physiological temperature (PT). In the slices prepared at CT, we found significant
    spine loss and reconstruction, synaptic vesicle rearrangement and decrease in
    synaptic proteins, all of which were not detected in slices prepared at PT. Consistent
    with these structural findings, slices prepared at PT showed higher release probability.
    Furthermore, preparation at PT allows electrophysiological recording immediately
    after slicing resulting in higher detectability of long-term depression (LTD)
    after motor learning compared with that at CT. These results indicate substantial
    advantages of the slice preparation at PT for investigating synaptic functions
    in different physiological conditions.
article_number: '63'
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Kohgaku
  full_name: Eguchi, Kohgaku
  id: 2B7846DC-F248-11E8-B48F-1D18A9856A87
  last_name: Eguchi
  orcid: 0000-0002-6170-2546
- first_name: Philipp
  full_name: Velicky, Philipp
  id: 39BDC62C-F248-11E8-B48F-1D18A9856A87
  last_name: Velicky
  orcid: 0000-0002-2340-7431
- first_name: Elena
  full_name: Hollergschwandtner, Elena
  id: 3C054040-F248-11E8-B48F-1D18A9856A87
  last_name: Hollergschwandtner
- first_name: Makoto
  full_name: Itakura, Makoto
  last_name: Itakura
- first_name: Yugo
  full_name: Fukazawa, Yugo
  last_name: Fukazawa
- first_name: Johann G
  full_name: Danzl, Johann G
  id: 42EFD3B6-F248-11E8-B48F-1D18A9856A87
  last_name: Danzl
  orcid: 0000-0001-8559-3973
- first_name: Ryuichi
  full_name: Shigemoto, Ryuichi
  id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
  last_name: Shigemoto
  orcid: 0000-0001-8761-9444
citation:
  ama: Eguchi K, Velicky P, Saeckl E, et al. Advantages of acute brain slices prepared
    at physiological temperature in the characterization of synaptic functions. <i>Frontiers
    in Cellular Neuroscience</i>. 2020;14. doi:<a href="https://doi.org/10.3389/fncel.2020.00063">10.3389/fncel.2020.00063</a>
  apa: Eguchi, K., Velicky, P., Saeckl, E., Itakura, M., Fukazawa, Y., Danzl, J. G.,
    &#38; Shigemoto, R. (2020). Advantages of acute brain slices prepared at physiological
    temperature in the characterization of synaptic functions. <i>Frontiers in Cellular
    Neuroscience</i>. Frontiers Media. <a href="https://doi.org/10.3389/fncel.2020.00063">https://doi.org/10.3389/fncel.2020.00063</a>
  chicago: Eguchi, Kohgaku, Philipp Velicky, Elena Saeckl, Makoto Itakura, Yugo Fukazawa,
    Johann G Danzl, and Ryuichi Shigemoto. “Advantages of Acute Brain Slices Prepared
    at Physiological Temperature in the Characterization of Synaptic Functions.” <i>Frontiers
    in Cellular Neuroscience</i>. Frontiers Media, 2020. <a href="https://doi.org/10.3389/fncel.2020.00063">https://doi.org/10.3389/fncel.2020.00063</a>.
  ieee: K. Eguchi <i>et al.</i>, “Advantages of acute brain slices prepared at physiological
    temperature in the characterization of synaptic functions,” <i>Frontiers in Cellular
    Neuroscience</i>, vol. 14. Frontiers Media, 2020.
  ista: Eguchi K, Velicky P, Saeckl E, Itakura M, Fukazawa Y, Danzl JG, Shigemoto
    R. 2020. Advantages of acute brain slices prepared at physiological temperature
    in the characterization of synaptic functions. Frontiers in Cellular Neuroscience.
    14, 63.
  mla: Eguchi, Kohgaku, et al. “Advantages of Acute Brain Slices Prepared at Physiological
    Temperature in the Characterization of Synaptic Functions.” <i>Frontiers in Cellular
    Neuroscience</i>, vol. 14, 63, Frontiers Media, 2020, doi:<a href="https://doi.org/10.3389/fncel.2020.00063">10.3389/fncel.2020.00063</a>.
  short: K. Eguchi, P. Velicky, E. Saeckl, M. Itakura, Y. Fukazawa, J.G. Danzl, R.
    Shigemoto, Frontiers in Cellular Neuroscience 14 (2020).
date_created: 2020-04-19T22:00:55Z
date_published: 2020-03-19T00:00:00Z
date_updated: 2023-08-21T06:12:48Z
day: '19'
ddc:
- '570'
department:
- _id: JoDa
- _id: RySh
doi: 10.3389/fncel.2020.00063
ec_funded: 1
external_id:
  isi:
  - '000525582200001'
file:
- access_level: open_access
  checksum: 1c145123c6f8dc3e2e4bd5a66a1ad60e
  content_type: application/pdf
  creator: dernst
  date_created: 2020-04-20T10:59:49Z
  date_updated: 2020-07-14T12:48:01Z
  file_id: '7668'
  file_name: 2020_FrontiersCellularNeurosc_Eguchi.pdf
  file_size: 9227283
  relation: main_file
file_date_updated: 2020-07-14T12:48:01Z
has_accepted_license: '1'
intvolume: '        14'
isi: 1
language:
- iso: eng
month: '03'
oa: 1
oa_version: Published Version
project:
- _id: 2659CC84-B435-11E9-9278-68D0E5697425
  call_identifier: H2020
  grant_number: '793482'
  name: 'Ultrastructural analysis of phosphoinositides in nerve terminals: distribution,
    dynamics and physiological roles in synaptic transmission'
- _id: 25CA28EA-B435-11E9-9278-68D0E5697425
  call_identifier: H2020
  grant_number: '694539'
  name: 'In situ analysis of single channel subunit composition in neurons: physiological
    implication in synaptic plasticity and behaviour'
- _id: 265CB4D0-B435-11E9-9278-68D0E5697425
  call_identifier: FWF
  grant_number: I03600
  name: Optical control of synaptic function via adhesion molecules
- _id: B67AFEDC-15C9-11EA-A837-991A96BB2854
  name: IST Austria Open Access Fund
publication: Frontiers in Cellular Neuroscience
publication_identifier:
  issn:
  - '16625102'
publication_status: published
publisher: Frontiers Media
quality_controlled: '1'
scopus_import: '1'
status: public
title: Advantages of acute brain slices prepared at physiological temperature in the
  characterization of synaptic functions
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 14
year: '2020'
...
---
_id: '41'
abstract:
- lang: eng
  text: 'The small-conductance, Ca2+-activated K+ (SK) channel subtype SK2 regulates
    the spike rate and firing frequency, as well as Ca2+ transients in Purkinje cells
    (PCs). To understand the molecular basis by which SK2 channels mediate these functions,
    we analyzed the exact location and densities of SK2 channels along the neuronal
    surface of the mouse cerebellar PCs using SDS-digested freeze-fracture replica
    labeling (SDS-FRL) of high sensitivity combined with quantitative analyses. Immunogold
    particles for SK2 were observed on post- and pre-synaptic compartments showing
    both scattered and clustered distribution patterns. We found an axo-somato-dendritic
    gradient of the SK2 particle density increasing 12-fold from soma to dendritic
    spines. Using two different immunogold approaches, we also found that SK2 immunoparticles
    were frequently adjacent to, but never overlap with, the postsynaptic density
    of excitatory synapses in PC spines. Co-immunoprecipitation analysis demonstrated
    that SK2 channels form macromolecular complexes with two types of proteins that
    mobilize Ca2+: CaV2.1 channels and mGlu1α receptors in the cerebellum. Freeze-fracture
    replica double-labeling showed significant co-clustering of particles for SK2
    with those for CaV2.1 channels and mGlu1α receptors. SK2 channels were also detected
    at presynaptic sites, mostly at the presynaptic active zone (AZ), where they are
    close to CaV2.1 channels, though they are not significantly co-clustered. These
    data demonstrate that SK2 channels located in different neuronal compartments
    can associate with distinct proteins mobilizing Ca2+, and suggest that the ultrastructural
    association of SK2 with CaV2.1 and mGlu1α provides the mechanism that ensures
    voltage (excitability) regulation by distinct intracellular Ca2+ transients in
    PCs.'
article_number: '311'
article_processing_charge: No
article_type: original
author:
- first_name: Rafæl
  full_name: Luján, Rafæl
  last_name: Luján
- first_name: Carolina
  full_name: Aguado, Carolina
  last_name: Aguado
- first_name: Francisco
  full_name: Ciruela, Francisco
  last_name: Ciruela
- first_name: Xavier
  full_name: Arus, Xavier
  last_name: Arus
- first_name: Alejandro
  full_name: Martín Belmonte, Alejandro
  last_name: Martín Belmonte
- first_name: Rocío
  full_name: Alfaro Ruiz, Rocío
  last_name: Alfaro Ruiz
- first_name: Jesus
  full_name: Martinez Gomez, Jesus
  last_name: Martinez Gomez
- first_name: Luis
  full_name: De La Ossa, Luis
  last_name: De La Ossa
- first_name: Masahiko
  full_name: Watanabe, Masahiko
  last_name: Watanabe
- first_name: John
  full_name: Adelman, John
  last_name: Adelman
- first_name: Ryuichi
  full_name: Shigemoto, Ryuichi
  id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
  last_name: Shigemoto
  orcid: 0000-0001-8761-9444
- first_name: Yugo
  full_name: Fukazawa, Yugo
  last_name: Fukazawa
citation:
  ama: Luján R, Aguado C, Ciruela F, et al. Sk2 channels associate with mGlu1α receptors
    and CaV2.1 channels in Purkinje cells. <i>Frontiers in Cellular Neuroscience</i>.
    2018;12. doi:<a href="https://doi.org/10.3389/fncel.2018.00311">10.3389/fncel.2018.00311</a>
  apa: Luján, R., Aguado, C., Ciruela, F., Arus, X., Martín Belmonte, A., Alfaro Ruiz,
    R., … Fukazawa, Y. (2018). Sk2 channels associate with mGlu1α receptors and CaV2.1
    channels in Purkinje cells. <i>Frontiers in Cellular Neuroscience</i>. Frontiers
    Media. <a href="https://doi.org/10.3389/fncel.2018.00311">https://doi.org/10.3389/fncel.2018.00311</a>
  chicago: Luján, Rafæl, Carolina Aguado, Francisco Ciruela, Xavier Arus, Alejandro
    Martín Belmonte, Rocío Alfaro Ruiz, Jesus Martinez Gomez, et al. “Sk2 Channels
    Associate with MGlu1α Receptors and CaV2.1 Channels in Purkinje Cells.” <i>Frontiers
    in Cellular Neuroscience</i>. Frontiers Media, 2018. <a href="https://doi.org/10.3389/fncel.2018.00311">https://doi.org/10.3389/fncel.2018.00311</a>.
  ieee: R. Luján <i>et al.</i>, “Sk2 channels associate with mGlu1α receptors and
    CaV2.1 channels in Purkinje cells,” <i>Frontiers in Cellular Neuroscience</i>,
    vol. 12. Frontiers Media, 2018.
  ista: Luján R, Aguado C, Ciruela F, Arus X, Martín Belmonte A, Alfaro Ruiz R, Martinez
    Gomez J, De La Ossa L, Watanabe M, Adelman J, Shigemoto R, Fukazawa Y. 2018. Sk2
    channels associate with mGlu1α receptors and CaV2.1 channels in Purkinje cells.
    Frontiers in Cellular Neuroscience. 12, 311.
  mla: Luján, Rafæl, et al. “Sk2 Channels Associate with MGlu1α Receptors and CaV2.1
    Channels in Purkinje Cells.” <i>Frontiers in Cellular Neuroscience</i>, vol. 12,
    311, Frontiers Media, 2018, doi:<a href="https://doi.org/10.3389/fncel.2018.00311">10.3389/fncel.2018.00311</a>.
  short: R. Luján, C. Aguado, F. Ciruela, X. Arus, A. Martín Belmonte, R. Alfaro Ruiz,
    J. Martinez Gomez, L. De La Ossa, M. Watanabe, J. Adelman, R. Shigemoto, Y. Fukazawa,
    Frontiers in Cellular Neuroscience 12 (2018).
date_created: 2018-12-11T11:44:19Z
date_published: 2018-09-19T00:00:00Z
date_updated: 2023-09-18T09:31:18Z
day: '19'
ddc:
- '570'
department:
- _id: RySh
doi: 10.3389/fncel.2018.00311
ec_funded: 1
external_id:
  isi:
  - '000445090100002'
file:
- access_level: open_access
  checksum: 0bcaec8d596162af0b7fe3f31325d480
  content_type: application/pdf
  creator: dernst
  date_created: 2018-12-17T08:49:03Z
  date_updated: 2020-07-14T12:46:23Z
  file_id: '5684'
  file_name: fncel-12-00311.pdf
  file_size: 6834251
  relation: main_file
file_date_updated: 2020-07-14T12:46:23Z
has_accepted_license: '1'
intvolume: '        12'
isi: 1
language:
- iso: eng
month: '09'
oa: 1
oa_version: Published Version
project:
- _id: 25CBA828-B435-11E9-9278-68D0E5697425
  call_identifier: H2020
  grant_number: '720270'
  name: Human Brain Project Specific Grant Agreement 1 (HBP SGA 1)
publication: Frontiers in Cellular Neuroscience
publication_identifier:
  issn:
  - '16625102'
publication_status: published
publisher: Frontiers Media
publist_id: '8013'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Sk2 channels associate with mGlu1α receptors and CaV2.1 channels in Purkinje
  cells
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 12
year: '2018'
...
---
_id: '960'
abstract:
- lang: eng
  text: The human cerebral cortex is the seat of our cognitive abilities and composed
    of an extraordinary number of neurons, organized in six distinct layers. The establishment
    of specific morphological and physiological features in individual neurons needs
    to be regulated with high precision. Impairments in the sequential developmental
    programs instructing corticogenesis lead to alterations in the cortical cytoarchitecture
    which is thought to represent the major underlying cause for several neurological
    disorders including neurodevelopmental and psychiatric diseases. In this review
    we discuss the role of cell polarity at sequential stages during cortex development.
    We first provide an overview of morphological cell polarity features in cortical
    neural stem cells and newly-born postmitotic neurons. We then synthesize a conceptual
    molecular and biochemical framework how cell polarity is established at the cellular
    level through a break in symmetry in nascent cortical projection neurons. Lastly
    we provide a perspective how the molecular mechanisms applying to single cells
    could be probed and integrated in an in vivo and tissue-wide context.
article_number: '176'
article_processing_charge: Yes
author:
- first_name: Andi H
  full_name: Hansen, Andi H
  id: 38853E16-F248-11E8-B48F-1D18A9856A87
  last_name: Hansen
- first_name: Christian F
  full_name: Düllberg, Christian F
  id: 459064DC-F248-11E8-B48F-1D18A9856A87
  last_name: Düllberg
  orcid: 0000-0001-6335-9748
- first_name: Christine
  full_name: Mieck, Christine
  id: 34CAE85C-F248-11E8-B48F-1D18A9856A87
  last_name: Mieck
  orcid: 0000-0003-1919-7416
- first_name: Martin
  full_name: Loose, Martin
  id: 462D4284-F248-11E8-B48F-1D18A9856A87
  last_name: Loose
  orcid: 0000-0001-7309-9724
- first_name: Simon
  full_name: Hippenmeyer, Simon
  id: 37B36620-F248-11E8-B48F-1D18A9856A87
  last_name: Hippenmeyer
  orcid: 0000-0003-2279-1061
citation:
  ama: Hansen AH, Düllberg CF, Mieck C, Loose M, Hippenmeyer S. Cell polarity in cerebral
    cortex development - cellular architecture shaped by biochemical networks. <i>Frontiers
    in Cellular Neuroscience</i>. 2017;11. doi:<a href="https://doi.org/10.3389/fncel.2017.00176">10.3389/fncel.2017.00176</a>
  apa: Hansen, A. H., Düllberg, C. F., Mieck, C., Loose, M., &#38; Hippenmeyer, S.
    (2017). Cell polarity in cerebral cortex development - cellular architecture shaped
    by biochemical networks. <i>Frontiers in Cellular Neuroscience</i>. Frontiers
    Research Foundation. <a href="https://doi.org/10.3389/fncel.2017.00176">https://doi.org/10.3389/fncel.2017.00176</a>
  chicago: Hansen, Andi H, Christian F Düllberg, Christine Mieck, Martin Loose, and
    Simon Hippenmeyer. “Cell Polarity in Cerebral Cortex Development - Cellular Architecture
    Shaped by Biochemical Networks.” <i>Frontiers in Cellular Neuroscience</i>. Frontiers
    Research Foundation, 2017. <a href="https://doi.org/10.3389/fncel.2017.00176">https://doi.org/10.3389/fncel.2017.00176</a>.
  ieee: A. H. Hansen, C. F. Düllberg, C. Mieck, M. Loose, and S. Hippenmeyer, “Cell
    polarity in cerebral cortex development - cellular architecture shaped by biochemical
    networks,” <i>Frontiers in Cellular Neuroscience</i>, vol. 11. Frontiers Research
    Foundation, 2017.
  ista: Hansen AH, Düllberg CF, Mieck C, Loose M, Hippenmeyer S. 2017. Cell polarity
    in cerebral cortex development - cellular architecture shaped by biochemical networks.
    Frontiers in Cellular Neuroscience. 11, 176.
  mla: Hansen, Andi H., et al. “Cell Polarity in Cerebral Cortex Development - Cellular
    Architecture Shaped by Biochemical Networks.” <i>Frontiers in Cellular Neuroscience</i>,
    vol. 11, 176, Frontiers Research Foundation, 2017, doi:<a href="https://doi.org/10.3389/fncel.2017.00176">10.3389/fncel.2017.00176</a>.
  short: A.H. Hansen, C.F. Düllberg, C. Mieck, M. Loose, S. Hippenmeyer, Frontiers
    in Cellular Neuroscience 11 (2017).
date_created: 2018-12-11T11:49:25Z
date_published: 2017-06-28T00:00:00Z
date_updated: 2024-03-25T23:30:23Z
day: '28'
ddc:
- '570'
department:
- _id: SiHi
- _id: MaLo
doi: 10.3389/fncel.2017.00176
ec_funded: 1
external_id:
  isi:
  - '000404486700001'
file:
- access_level: open_access
  checksum: dc1f5a475b918d09a0f9f587400b1626
  content_type: application/pdf
  creator: system
  date_created: 2018-12-12T10:09:40Z
  date_updated: 2020-07-14T12:48:16Z
  file_id: '4764'
  file_name: IST-2017-830-v1+1_2017_Hansen_CellPolarity.pdf
  file_size: 2153858
  relation: main_file
file_date_updated: 2020-07-14T12:48:16Z
has_accepted_license: '1'
intvolume: '        11'
isi: 1
language:
- iso: eng
month: '06'
oa: 1
oa_version: Published Version
project:
- _id: 25D61E48-B435-11E9-9278-68D0E5697425
  call_identifier: FP7
  grant_number: '618444'
  name: Molecular Mechanisms of Cerebral Cortex Development
- _id: 25D7962E-B435-11E9-9278-68D0E5697425
  grant_number: RGP0053/2014
  name: Quantitative Structure-Function Analysis of Cerebral Cortex Assembly at Clonal
    Level
- _id: 25681D80-B435-11E9-9278-68D0E5697425
  call_identifier: FP7
  grant_number: '291734'
  name: International IST Postdoc Fellowship Programme
- _id: 25985A36-B435-11E9-9278-68D0E5697425
  call_identifier: FWF
  grant_number: T00817-B21
  name: The biochemical basis of PAR polarization
publication: Frontiers in Cellular Neuroscience
publication_identifier:
  issn:
  - '16625102'
publication_status: published
publisher: Frontiers Research Foundation
publist_id: '6445'
pubrep_id: '830'
quality_controlled: '1'
related_material:
  record:
  - id: '9962'
    relation: dissertation_contains
    status: public
scopus_import: '1'
status: public
title: Cell polarity in cerebral cortex development - cellular architecture shaped
  by biochemical networks
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 11
year: '2017'
...