@article{7416,
  abstract     = {Earlier, we demonstrated that transcript levels of METAL TOLERANCE PROTEIN2 (MTP2) and of HEAVY METAL ATPase2 (HMA2) increase strongly in roots of Arabidopsis upon prolonged zinc (Zn) deficiency and respond to shoot physiological Zn status, and not to the local Zn status in roots. This provided evidence for shoot-to-root communication in the acclimation of plants to Zn deficiency. Zn-deficient soils limit both the yield and quality of agricultural crops and can result in clinically relevant nutritional Zn deficiency in human populations. Implementing Zn deficiency during cultivation of the model plant Arabidopsis thaliana on agar-solidified media is difficult because trace element contaminations are present in almost all commercially available agars. Here, we demonstrate root morphological acclimations to Zn deficiency on agar-solidified medium following the effective removal of contaminants. These advancements allow reproducible phenotyping toward understanding fundamental plant responses to deficiencies of Zn and other essential trace elements.},
  author       = {Sinclair, Scott A and Krämer, U.},
  issn         = {1559-2324},
  journal      = {Plant Signaling & Behavior},
  number       = {1},
  publisher    = {Taylor & Francis},
  title        = {{Generation of effective zinc-deficient agar-solidified media allows identification of root morphology changes in response to zinc limitation}},
  doi          = {10.1080/15592324.2019.1687175},
  volume       = {15},
  year         = {2020},
}

@article{7417,
  abstract     = {Previously, we reported that the allelic de-etiolated by zinc (dez) and trichome birefringence (tbr) mutants exhibit photomorphogenic development in the dark, which is enhanced by high Zn. TRICHOME BIREFRINGENCE-LIKE proteins had been implicated in transferring acetyl groups to various hemicelluloses. Pectin O-acetylation levels were lower in dark-grown dez seedlings than in the wild type. We observed Zn-enhanced photomorphogenesis in the dark also in the reduced wall acetylation 2 (rwa2-3) mutant, which exhibits lowered O-acetylation levels of cell wall macromolecules including pectins and xyloglucans, supporting a role for cell wall macromolecule O-acetylation in the photomorphogenic phenotypes of rwa2-3 and dez. Application of very short oligogalacturonides (vsOGs) restored skotomorphogenesis in dark-grown dez and rwa2-3. Here we demonstrate that in dez, O-acetylation of non-pectin cell wall components, notably of xyloglucan, is enhanced. Our results highlight the complexity of cell wall homeostasis and indicate against an influence of xyloglucan O-acetylation on light-dependent seedling development.},
  author       = {Sinclair, Scott A and Gille, S. and Pauly, M. and Krämer, U.},
  issn         = {1559-2324},
  journal      = {Plant Signaling & Behavior},
  number       = {1},
  publisher    = {Informa UK Limited},
  title        = {{Regulation of acetylation of plant cell wall components is complex and responds to external stimuli}},
  doi          = {10.1080/15592324.2019.1687185},
  volume       = {15},
  year         = {2020},
}