---
_id: '8234'
abstract:
- lang: eng
  text: Molecular imaging probes such as PET-tracers have the potential to improve
    the accuracy of tumor characterization by directly visualizing the biochemical
    situation. Thus, molecular changes can be detected early before morphological
    manifestation. The A3 adenosine receptor (A3AR) is described to be highly expressed
    in colon cancer cell lines and human colorectal cancer (CRC), suggesting this
    receptor as a tumor marker. The aim of this preclinical study was the evaluation
    of FE@SUPPY as a PET-tracer for CRC using in vitro imaging and in vivo PET imaging.
    First, affinity and selectivity of FE@SUPPY and its metabolites were determined,
    proving the favorable binding profile of FE@SUPPY. The human adenocarcinoma cell
    line HT-29 was characterized regarding its hA3AR expression and was subsequently
    chosen as tumor graft. Promising results regarding the potential of FE@SUPPY as
    a PET-tracer for CRC imaging were obtained by autoradiography as ≥2.3-fold higher
    accumulation of FE@SUPPY was found in CRC tissue compared to adjacent healthy
    colon tissue from the same patient. Nevertheless, first in vivo studies using
    HT-29 xenografts showed insufficient tumor uptake due to (1) poor conservation
    of target expression in xenografts and (2) unfavorable pharmacokinetics of FE@SUPPY
    in mice. We therefore conclude that HT-29 xenografts are not adequate to visualize
    hA3ARs using FE@SUPPY.
article_number: '1269830'
article_processing_charge: No
article_type: original
author:
- first_name: T.
  full_name: Balber, T.
  last_name: Balber
- first_name: Judit
  full_name: Singer, Judit
  id: 36432834-F248-11E8-B48F-1D18A9856A87
  last_name: Singer
  orcid: 0000-0002-8777-3502
- first_name: N.
  full_name: Berroterán-Infante, N.
  last_name: Berroterán-Infante
- first_name: M.
  full_name: Dumanic, M.
  last_name: Dumanic
- first_name: L.
  full_name: Fetty, L.
  last_name: Fetty
- first_name: J.
  full_name: Fazekas-Singer, J.
  last_name: Fazekas-Singer
  orcid: 0000-0002-8777-3502
- first_name: C.
  full_name: Vraka, C.
  last_name: Vraka
- first_name: L.
  full_name: Nics, L.
  last_name: Nics
- first_name: M.
  full_name: Bergmann, M.
  last_name: Bergmann
- first_name: K.
  full_name: Pallitsch, K.
  last_name: Pallitsch
- first_name: H.
  full_name: Spreitzer, H.
  last_name: Spreitzer
- first_name: W.
  full_name: Wadsak, W.
  last_name: Wadsak
  orcid: 0000-0003-4479-8053
- first_name: M.
  full_name: Hacker, M.
  last_name: Hacker
- first_name: E.
  full_name: Jensen-Jarolim, E.
  last_name: Jensen-Jarolim
- first_name: H.
  full_name: Viernstein, H.
  last_name: Viernstein
- first_name: M.
  full_name: Mitterhauser, M.
  last_name: Mitterhauser
  orcid: 0000-0003-3173-5272
citation:
  ama: 'Balber T, Singer J, Berroterán-Infante N, et al. Preclinical in vitro and
    in vivo evaluation of [18F]FE@SUPPY for cancer PET imaging: Limitations of a xenograft
    model for colorectal cancer. <i>Contrast Media &#38; Molecular Imaging</i>. 2018;2018.
    doi:<a href="https://doi.org/10.1155/2018/1269830">10.1155/2018/1269830</a>'
  apa: 'Balber, T., Singer, J., Berroterán-Infante, N., Dumanic, M., Fetty, L., Fazekas-Singer,
    J., … Mitterhauser, M. (2018). Preclinical in vitro and in vivo evaluation of
    [18F]FE@SUPPY for cancer PET imaging: Limitations of a xenograft model for colorectal
    cancer. <i>Contrast Media &#38; Molecular Imaging</i>. Hindawi. <a href="https://doi.org/10.1155/2018/1269830">https://doi.org/10.1155/2018/1269830</a>'
  chicago: 'Balber, T., Judit Singer, N. Berroterán-Infante, M. Dumanic, L. Fetty,
    J. Fazekas-Singer, C. Vraka, et al. “Preclinical in Vitro and in Vivo Evaluation
    of [18F]FE@SUPPY for Cancer PET Imaging: Limitations of a Xenograft Model for
    Colorectal Cancer.” <i>Contrast Media &#38; Molecular Imaging</i>. Hindawi, 2018.
    <a href="https://doi.org/10.1155/2018/1269830">https://doi.org/10.1155/2018/1269830</a>.'
  ieee: 'T. Balber <i>et al.</i>, “Preclinical in vitro and in vivo evaluation of
    [18F]FE@SUPPY for cancer PET imaging: Limitations of a xenograft model for colorectal
    cancer,” <i>Contrast Media &#38; Molecular Imaging</i>, vol. 2018. Hindawi, 2018.'
  ista: 'Balber T, Singer J, Berroterán-Infante N, Dumanic M, Fetty L, Fazekas-Singer
    J, Vraka C, Nics L, Bergmann M, Pallitsch K, Spreitzer H, Wadsak W, Hacker M,
    Jensen-Jarolim E, Viernstein H, Mitterhauser M. 2018. Preclinical in vitro and
    in vivo evaluation of [18F]FE@SUPPY for cancer PET imaging: Limitations of a xenograft
    model for colorectal cancer. Contrast Media &#38; Molecular Imaging. 2018, 1269830.'
  mla: 'Balber, T., et al. “Preclinical in Vitro and in Vivo Evaluation of [18F]FE@SUPPY
    for Cancer PET Imaging: Limitations of a Xenograft Model for Colorectal Cancer.”
    <i>Contrast Media &#38; Molecular Imaging</i>, vol. 2018, 1269830, Hindawi, 2018,
    doi:<a href="https://doi.org/10.1155/2018/1269830">10.1155/2018/1269830</a>.'
  short: T. Balber, J. Singer, N. Berroterán-Infante, M. Dumanic, L. Fetty, J. Fazekas-Singer,
    C. Vraka, L. Nics, M. Bergmann, K. Pallitsch, H. Spreitzer, W. Wadsak, M. Hacker,
    E. Jensen-Jarolim, H. Viernstein, M. Mitterhauser, Contrast Media &#38; Molecular
    Imaging 2018 (2018).
date_created: 2020-08-10T11:53:07Z
date_published: 2018-02-13T00:00:00Z
date_updated: 2021-01-12T08:17:38Z
day: '13'
doi: 10.1155/2018/1269830
extern: '1'
intvolume: '      2018'
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: https://doi.org/10.1155/2018/1269830
month: '02'
oa: 1
oa_version: Published Version
publication: Contrast Media & Molecular Imaging
publication_identifier:
  issn:
  - 1555-4309
  - 1555-4317
publication_status: published
publisher: Hindawi
quality_controlled: '1'
status: public
title: 'Preclinical in vitro and in vivo evaluation of [18F]FE@SUPPY for cancer PET
  imaging: Limitations of a xenograft model for colorectal cancer'
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 2018
year: '2018'
...