---
_id: '8439'
abstract:
- lang: eng
  text: Lipopolysaccharides (LPS) are complex glycolipids forming the outside layer
    of Gram-negative bacteria. Their hydrophobic and heterogeneous nature greatly
    hampers their structural study in an environment similar to the bacterial surface.
    We have studied LPS purified from E. coli and pathogenic P. aeruginosa with long
    O-antigen polysaccharides assembled in solution as vesicles or elongated micelles.
    Solid-state NMR with magic-angle spinning permitted the identification of NMR
    signals arising from regions with different flexibilities in the LPS, from the
    lipid components to the O-antigen polysaccharides. Atomic scale data on the LPS
    enabled the study of the interaction of gentamicin antibiotic bound to P. aeruginosa
    LPS, for which we could confirm that a specific oligosaccharide is involved in
    the antibiotic binding. The possibility to study LPS alone and bound to a ligand
    when it is assembled in membrane-like structures opens great prospects for the
    investigation of proteins and antibiotics that specifically target such an important
    molecule at the surface of Gram-negative bacteria.
article_processing_charge: No
article_type: original
author:
- first_name: Cedric
  full_name: Laguri, Cedric
  last_name: Laguri
- first_name: Alba
  full_name: Silipo, Alba
  last_name: Silipo
- first_name: Alessandra M.
  full_name: Martorana, Alessandra M.
  last_name: Martorana
- first_name: Paul
  full_name: Schanda, Paul
  id: 7B541462-FAF6-11E9-A490-E8DFE5697425
  last_name: Schanda
  orcid: 0000-0002-9350-7606
- first_name: Roberta
  full_name: Marchetti, Roberta
  last_name: Marchetti
- first_name: Alessandra
  full_name: Polissi, Alessandra
  last_name: Polissi
- first_name: Antonio
  full_name: Molinaro, Antonio
  last_name: Molinaro
- first_name: Jean-Pierre
  full_name: Simorre, Jean-Pierre
  last_name: Simorre
citation:
  ama: Laguri C, Silipo A, Martorana AM, et al. Solid state NMR studies of intact
    lipopolysaccharide endotoxin. <i>ACS Chemical Biology</i>. 2018;13(8):2106-2113.
    doi:<a href="https://doi.org/10.1021/acschembio.8b00271">10.1021/acschembio.8b00271</a>
  apa: Laguri, C., Silipo, A., Martorana, A. M., Schanda, P., Marchetti, R., Polissi,
    A., … Simorre, J.-P. (2018). Solid state NMR studies of intact lipopolysaccharide
    endotoxin. <i>ACS Chemical Biology</i>. American Chemical Society. <a href="https://doi.org/10.1021/acschembio.8b00271">https://doi.org/10.1021/acschembio.8b00271</a>
  chicago: Laguri, Cedric, Alba Silipo, Alessandra M. Martorana, Paul Schanda, Roberta
    Marchetti, Alessandra Polissi, Antonio Molinaro, and Jean-Pierre Simorre. “Solid
    State NMR Studies of Intact Lipopolysaccharide Endotoxin.” <i>ACS Chemical Biology</i>.
    American Chemical Society, 2018. <a href="https://doi.org/10.1021/acschembio.8b00271">https://doi.org/10.1021/acschembio.8b00271</a>.
  ieee: C. Laguri <i>et al.</i>, “Solid state NMR studies of intact lipopolysaccharide
    endotoxin,” <i>ACS Chemical Biology</i>, vol. 13, no. 8. American Chemical Society,
    pp. 2106–2113, 2018.
  ista: Laguri C, Silipo A, Martorana AM, Schanda P, Marchetti R, Polissi A, Molinaro
    A, Simorre J-P. 2018. Solid state NMR studies of intact lipopolysaccharide endotoxin.
    ACS Chemical Biology. 13(8), 2106–2113.
  mla: Laguri, Cedric, et al. “Solid State NMR Studies of Intact Lipopolysaccharide
    Endotoxin.” <i>ACS Chemical Biology</i>, vol. 13, no. 8, American Chemical Society,
    2018, pp. 2106–13, doi:<a href="https://doi.org/10.1021/acschembio.8b00271">10.1021/acschembio.8b00271</a>.
  short: C. Laguri, A. Silipo, A.M. Martorana, P. Schanda, R. Marchetti, A. Polissi,
    A. Molinaro, J.-P. Simorre, ACS Chemical Biology 13 (2018) 2106–2113.
date_created: 2020-09-18T10:05:09Z
date_published: 2018-07-02T00:00:00Z
date_updated: 2021-01-12T08:19:16Z
day: '02'
doi: 10.1021/acschembio.8b00271
extern: '1'
intvolume: '        13'
issue: '8'
keyword:
- Molecular Medicine
- Biochemistry
- General Medicine
language:
- iso: eng
month: '07'
oa_version: None
page: 2106-2113
publication: ACS Chemical Biology
publication_identifier:
  issn:
  - 1554-8929
  - 1554-8937
publication_status: published
publisher: American Chemical Society
quality_controlled: '1'
status: public
title: Solid state NMR studies of intact lipopolysaccharide endotoxin
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 13
year: '2018'
...