---
_id: '9046'
acknowledgement: Our work was supported by the Swedish Research Council (grant 2017-01527)
  to DIA
article_number: e1009172
article_processing_charge: No
article_type: original
author:
- first_name: Roderich
  full_name: Römhild, Roderich
  id: 68E56E44-62B0-11EA-B963-444F3DDC885E
  last_name: Römhild
  orcid: 0000-0001-9480-5261
- first_name: Dan I.
  full_name: Andersson, Dan I.
  last_name: Andersson
citation:
  ama: Römhild R, Andersson DI. Mechanisms and therapeutic potential of collateral
    sensitivity to antibiotics. <i>PLoS Pathogens</i>. 2021;17(1). doi:<a href="https://doi.org/10.1371/journal.ppat.1009172">10.1371/journal.ppat.1009172</a>
  apa: Römhild, R., &#38; Andersson, D. I. (2021). Mechanisms and therapeutic potential
    of collateral sensitivity to antibiotics. <i>PLoS Pathogens</i>. Public Library
    of Science. <a href="https://doi.org/10.1371/journal.ppat.1009172">https://doi.org/10.1371/journal.ppat.1009172</a>
  chicago: Römhild, Roderich, and Dan I. Andersson. “Mechanisms and Therapeutic Potential
    of Collateral Sensitivity to Antibiotics.” <i>PLoS Pathogens</i>. Public Library
    of Science, 2021. <a href="https://doi.org/10.1371/journal.ppat.1009172">https://doi.org/10.1371/journal.ppat.1009172</a>.
  ieee: R. Römhild and D. I. Andersson, “Mechanisms and therapeutic potential of collateral
    sensitivity to antibiotics,” <i>PLoS Pathogens</i>, vol. 17, no. 1. Public Library
    of Science, 2021.
  ista: Römhild R, Andersson DI. 2021. Mechanisms and therapeutic potential of collateral
    sensitivity to antibiotics. PLoS Pathogens. 17(1), e1009172.
  mla: Römhild, Roderich, and Dan I. Andersson. “Mechanisms and Therapeutic Potential
    of Collateral Sensitivity to Antibiotics.” <i>PLoS Pathogens</i>, vol. 17, no.
    1, e1009172, Public Library of Science, 2021, doi:<a href="https://doi.org/10.1371/journal.ppat.1009172">10.1371/journal.ppat.1009172</a>.
  short: R. Römhild, D.I. Andersson, PLoS Pathogens 17 (2021).
date_created: 2021-01-31T23:01:21Z
date_published: 2021-01-14T00:00:00Z
date_updated: 2023-08-07T13:36:55Z
day: '14'
ddc:
- '570'
department:
- _id: CaGu
doi: 10.1371/journal.ppat.1009172
external_id:
  isi:
  - '000610190400007'
  pmid:
  - '33444399'
file:
- access_level: open_access
  checksum: d745d7f8fcbb9b95fea16a36f94dee31
  content_type: application/pdf
  creator: dernst
  date_created: 2021-02-03T12:13:03Z
  date_updated: 2021-02-03T12:13:03Z
  file_id: '9070'
  file_name: 2021_PlosPathogens_Roemhild.pdf
  file_size: 570066
  relation: main_file
  success: 1
file_date_updated: 2021-02-03T12:13:03Z
has_accepted_license: '1'
intvolume: '        17'
isi: 1
issue: '1'
language:
- iso: eng
license: https://creativecommons.org/licenses/by/4.0/
month: '01'
oa: 1
oa_version: Published Version
pmid: 1
publication: PLoS Pathogens
publication_identifier:
  eissn:
  - '15537374'
  issn:
  - '15537366'
publication_status: published
publisher: Public Library of Science
quality_controlled: '1'
scopus_import: '1'
status: public
title: Mechanisms and therapeutic potential of collateral sensitivity to antibiotics
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 17
year: '2021'
...
---
_id: '540'
abstract:
- lang: eng
  text: RNA-dependent RNA polymerases (RdRps) play a key role in the life cycle of
    RNA viruses and impact their immunobiology. The arenavirus lymphocytic choriomeningitis
    virus (LCMV) strain Clone 13 provides a benchmark model for studying chronic infection.
    A major genetic determinant for its ability to persist maps to a single amino
    acid exchange in the viral L protein, which exhibits RdRp activity, yet its functional
    consequences remain elusive. To unravel the L protein interactions with the host
    proteome, we engineered infectious L protein-tagged LCMV virions by reverse genetics.
    A subsequent mass-spectrometric analysis of L protein pulldowns from infected
    human cells revealed a comprehensive network of interacting host proteins. The
    obtained LCMV L protein interactome was bioinformatically integrated with known
    host protein interactors of RdRps from other RNA viruses, emphasizing interconnected
    modules of human proteins. Functional characterization of selected interactors
    highlighted proviral (DDX3X) as well as antiviral (NKRF, TRIM21) host factors.
    To corroborate these findings, we infected Trim21-/-mice with LCMV and found impaired
    virus control in chronic infection. These results provide insights into the complex
    interactions of the arenavirus LCMV and other viral RdRps with the host proteome
    and contribute to a better molecular understanding of how chronic viruses interact
    with their host.
article_number: e1006758
author:
- first_name: Kseniya
  full_name: Khamina, Kseniya
  last_name: Khamina
- first_name: Alexander
  full_name: Lercher, Alexander
  last_name: Lercher
- first_name: Michael
  full_name: Caldera, Michael
  last_name: Caldera
- first_name: Christopher
  full_name: Schliehe, Christopher
  last_name: Schliehe
- first_name: Bojan
  full_name: Vilagos, Bojan
  last_name: Vilagos
- first_name: Mehmet
  full_name: Sahin, Mehmet
  last_name: Sahin
- first_name: Lindsay
  full_name: Kosack, Lindsay
  last_name: Kosack
- first_name: Anannya
  full_name: Bhattacharya, Anannya
  last_name: Bhattacharya
- first_name: Peter
  full_name: Májek, Peter
  last_name: Májek
- first_name: Alexey
  full_name: Stukalov, Alexey
  last_name: Stukalov
- first_name: Roberto
  full_name: Sacco, Roberto
  id: 42C9F57E-F248-11E8-B48F-1D18A9856A87
  last_name: Sacco
- first_name: Leo
  full_name: James, Leo
  last_name: James
- first_name: Daniel
  full_name: Pinschewer, Daniel
  last_name: Pinschewer
- first_name: Keiryn
  full_name: Bennett, Keiryn
  last_name: Bennett
- first_name: Jörg
  full_name: Menche, Jörg
  last_name: Menche
- first_name: Andreas
  full_name: Bergthaler, Andreas
  last_name: Bergthaler
citation:
  ama: Khamina K, Lercher A, Caldera M, et al. Characterization of host proteins interacting
    with the lymphocytic choriomeningitis virus L protein. <i>PLoS Pathogens</i>.
    2017;13(12). doi:<a href="https://doi.org/10.1371/journal.ppat.1006758">10.1371/journal.ppat.1006758</a>
  apa: Khamina, K., Lercher, A., Caldera, M., Schliehe, C., Vilagos, B., Sahin, M.,
    … Bergthaler, A. (2017). Characterization of host proteins interacting with the
    lymphocytic choriomeningitis virus L protein. <i>PLoS Pathogens</i>. Public Library
    of Science. <a href="https://doi.org/10.1371/journal.ppat.1006758">https://doi.org/10.1371/journal.ppat.1006758</a>
  chicago: Khamina, Kseniya, Alexander Lercher, Michael Caldera, Christopher Schliehe,
    Bojan Vilagos, Mehmet Sahin, Lindsay Kosack, et al. “Characterization of Host
    Proteins Interacting with the Lymphocytic Choriomeningitis Virus L Protein.” <i>PLoS
    Pathogens</i>. Public Library of Science, 2017. <a href="https://doi.org/10.1371/journal.ppat.1006758">https://doi.org/10.1371/journal.ppat.1006758</a>.
  ieee: K. Khamina <i>et al.</i>, “Characterization of host proteins interacting with
    the lymphocytic choriomeningitis virus L protein,” <i>PLoS Pathogens</i>, vol.
    13, no. 12. Public Library of Science, 2017.
  ista: Khamina K, Lercher A, Caldera M, Schliehe C, Vilagos B, Sahin M, Kosack L,
    Bhattacharya A, Májek P, Stukalov A, Sacco R, James L, Pinschewer D, Bennett K,
    Menche J, Bergthaler A. 2017. Characterization of host proteins interacting with
    the lymphocytic choriomeningitis virus L protein. PLoS Pathogens. 13(12), e1006758.
  mla: Khamina, Kseniya, et al. “Characterization of Host Proteins Interacting with
    the Lymphocytic Choriomeningitis Virus L Protein.” <i>PLoS Pathogens</i>, vol.
    13, no. 12, e1006758, Public Library of Science, 2017, doi:<a href="https://doi.org/10.1371/journal.ppat.1006758">10.1371/journal.ppat.1006758</a>.
  short: K. Khamina, A. Lercher, M. Caldera, C. Schliehe, B. Vilagos, M. Sahin, L.
    Kosack, A. Bhattacharya, P. Májek, A. Stukalov, R. Sacco, L. James, D. Pinschewer,
    K. Bennett, J. Menche, A. Bergthaler, PLoS Pathogens 13 (2017).
date_created: 2018-12-11T11:47:03Z
date_published: 2017-12-01T00:00:00Z
date_updated: 2021-01-12T08:01:48Z
day: '01'
ddc:
- '576'
- '616'
department:
- _id: GaNo
doi: 10.1371/journal.ppat.1006758
file:
- access_level: open_access
  checksum: 1aa20f19a1e90664fadce6e7d5284fdc
  content_type: application/pdf
  creator: system
  date_created: 2018-12-12T10:12:26Z
  date_updated: 2020-07-14T12:46:44Z
  file_id: '4944'
  file_name: IST-2018-931-v1+1_journal.ppat.1006758.pdf
  file_size: 4106772
  relation: main_file
file_date_updated: 2020-07-14T12:46:44Z
has_accepted_license: '1'
intvolume: '        13'
issue: '12'
language:
- iso: eng
month: '12'
oa: 1
oa_version: Published Version
publication: PLoS Pathogens
publication_identifier:
  issn:
  - '15537366'
publication_status: published
publisher: Public Library of Science
publist_id: '7276'
pubrep_id: '931'
quality_controlled: '1'
scopus_import: 1
status: public
title: Characterization of host proteins interacting with the lymphocytic choriomeningitis
  virus L protein
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 13
year: '2017'
...