---
_id: '7464'
abstract:
- lang: eng
  text: 'Retrovirus assembly is driven by the multidomain structural protein Gag.
    Interactions between the capsid domains (CA) of Gag result in Gag multimerization,
    leading to an immature virus particle that is formed by a protein lattice based
    on dimeric, trimeric, and hexameric protein contacts. Among retroviruses the inter-
    and intra-hexamer contacts differ, especially in the N-terminal sub-domain of
    CA (CANTD). For HIV-1 the cellular molecule inositol hexakisphosphate (IP6) interacts
    with and stabilizes the immature hexamer, and is required for production of infectious
    virus particles. We have used in vitro assembly, cryo-electron tomography and
    subtomogram averaging, atomistic molecular dynamics simulations and mutational
    analyses to study the HIV-related lentivirus equine infectious anemia virus (EIAV).
    In particular, we sought to understand the structural conservation of the immature
    lentivirus lattice and the role of IP6 in EIAV assembly. Similar to HIV-1, IP6
    strongly promoted in vitro assembly of EIAV Gag proteins into virus-like particles
    (VLPs), which took three morphologically highly distinct forms: narrow tubes,
    wide tubes, and spheres. Structural characterization of these VLPs to sub-4Å resolution
    unexpectedly showed that all three morphologies are based on an immature lattice
    with preserved key structural components, highlighting the structural versatility
    of CA to form immature assemblies. A direct comparison between EIAV and HIV revealed
    that both lentiviruses maintain similar immature interfaces, which are established
    by both conserved and non-conserved residues. In both EIAV and HIV-1, IP6 regulates
    immature assembly via conserved lysine residues within the CACTD and SP. Lastly,
    we demonstrate that IP6 stimulates in vitro assembly of immature particles of
    several other retroviruses in the lentivirus genus, suggesting a conserved role
    for IP6 in lentiviral assembly.'
acknowledged_ssus:
- _id: ScienComp
article_number: e1008277
article_processing_charge: No
article_type: original
author:
- first_name: Robert A.
  full_name: Dick, Robert A.
  last_name: Dick
- first_name: Chaoyi
  full_name: Xu, Chaoyi
  last_name: Xu
- first_name: Dustin R.
  full_name: Morado, Dustin R.
  last_name: Morado
- first_name: Vladyslav
  full_name: Kravchuk, Vladyslav
  id: 4D62F2A6-F248-11E8-B48F-1D18A9856A87
  last_name: Kravchuk
  orcid: 0000-0001-9523-9089
- first_name: Clifton L.
  full_name: Ricana, Clifton L.
  last_name: Ricana
- first_name: Terri D.
  full_name: Lyddon, Terri D.
  last_name: Lyddon
- first_name: Arianna M.
  full_name: Broad, Arianna M.
  last_name: Broad
- first_name: J. Ryan
  full_name: Feathers, J. Ryan
  last_name: Feathers
- first_name: Marc C.
  full_name: Johnson, Marc C.
  last_name: Johnson
- first_name: Volker M.
  full_name: Vogt, Volker M.
  last_name: Vogt
- first_name: Juan R.
  full_name: Perilla, Juan R.
  last_name: Perilla
- first_name: John A. G.
  full_name: Briggs, John A. G.
  last_name: Briggs
- first_name: Florian KM
  full_name: Schur, Florian KM
  id: 48AD8942-F248-11E8-B48F-1D18A9856A87
  last_name: Schur
  orcid: 0000-0003-4790-8078
citation:
  ama: Dick RA, Xu C, Morado DR, et al. Structures of immature EIAV Gag lattices reveal
    a conserved role for IP6 in lentivirus assembly. <i>PLOS Pathogens</i>. 2020;16(1).
    doi:<a href="https://doi.org/10.1371/journal.ppat.1008277">10.1371/journal.ppat.1008277</a>
  apa: Dick, R. A., Xu, C., Morado, D. R., Kravchuk, V., Ricana, C. L., Lyddon, T.
    D., … Schur, F. K. (2020). Structures of immature EIAV Gag lattices reveal a conserved
    role for IP6 in lentivirus assembly. <i>PLOS Pathogens</i>. Public Library of
    Science. <a href="https://doi.org/10.1371/journal.ppat.1008277">https://doi.org/10.1371/journal.ppat.1008277</a>
  chicago: Dick, Robert A., Chaoyi Xu, Dustin R. Morado, Vladyslav Kravchuk, Clifton
    L. Ricana, Terri D. Lyddon, Arianna M. Broad, et al. “Structures of Immature EIAV
    Gag Lattices Reveal a Conserved Role for IP6 in Lentivirus Assembly.” <i>PLOS
    Pathogens</i>. Public Library of Science, 2020. <a href="https://doi.org/10.1371/journal.ppat.1008277">https://doi.org/10.1371/journal.ppat.1008277</a>.
  ieee: R. A. Dick <i>et al.</i>, “Structures of immature EIAV Gag lattices reveal
    a conserved role for IP6 in lentivirus assembly,” <i>PLOS Pathogens</i>, vol.
    16, no. 1. Public Library of Science, 2020.
  ista: Dick RA, Xu C, Morado DR, Kravchuk V, Ricana CL, Lyddon TD, Broad AM, Feathers
    JR, Johnson MC, Vogt VM, Perilla JR, Briggs JAG, Schur FK. 2020. Structures of
    immature EIAV Gag lattices reveal a conserved role for IP6 in lentivirus assembly.
    PLOS Pathogens. 16(1), e1008277.
  mla: Dick, Robert A., et al. “Structures of Immature EIAV Gag Lattices Reveal a
    Conserved Role for IP6 in Lentivirus Assembly.” <i>PLOS Pathogens</i>, vol. 16,
    no. 1, e1008277, Public Library of Science, 2020, doi:<a href="https://doi.org/10.1371/journal.ppat.1008277">10.1371/journal.ppat.1008277</a>.
  short: R.A. Dick, C. Xu, D.R. Morado, V. Kravchuk, C.L. Ricana, T.D. Lyddon, A.M.
    Broad, J.R. Feathers, M.C. Johnson, V.M. Vogt, J.R. Perilla, J.A.G. Briggs, F.K.
    Schur, PLOS Pathogens 16 (2020).
date_created: 2020-02-06T18:47:17Z
date_published: 2020-01-27T00:00:00Z
date_updated: 2023-10-17T12:29:34Z
day: '27'
ddc:
- '570'
department:
- _id: FlSc
doi: 10.1371/journal.ppat.1008277
external_id:
  isi:
  - '000510746400010'
  pmid:
  - '31986188'
file:
- access_level: open_access
  checksum: a297f54d1fef0efe4789ca00f37f241e
  content_type: application/pdf
  creator: dernst
  date_created: 2020-02-11T10:07:28Z
  date_updated: 2020-07-14T12:47:59Z
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  file_name: 2020_PLOSPatho_Dick.pdf
  file_size: 4551246
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file_date_updated: 2020-07-14T12:47:59Z
has_accepted_license: '1'
intvolume: '        16'
isi: 1
issue: '1'
language:
- iso: eng
month: '01'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 26736D6A-B435-11E9-9278-68D0E5697425
  call_identifier: FWF
  grant_number: P31445
  name: Structural conservation and diversity in retroviral capsid
publication: PLOS Pathogens
publication_identifier:
  issn:
  - 1553-7374
publication_status: published
publisher: Public Library of Science
quality_controlled: '1'
related_material:
  record:
  - id: '9723'
    relation: research_data
    status: deleted
scopus_import: '1'
status: public
title: Structures of immature EIAV Gag lattices reveal a conserved role for IP6 in
  lentivirus assembly
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 16
year: '2020'
...