---
_id: '11057'
abstract:
- lang: eng
  text: During mitosis, transcription of genomic DNA is dramatically reduced, before
    it is reactivated during nuclear reformation in anaphase/telophase. Many aspects
    of the underlying principles that mediate transcriptional memory and reactivation
    in the daughter cells remain unclear. Here, we used ChIP-seq on synchronized cells
    at different stages after mitosis to generate genome-wide maps of histone modifications.
    Combined with EU-RNA-seq and Hi-C analyses, we found that during prometaphase,
    promoters, enhancers, and insulators retain H3K4me3 and H3K4me1, while losing
    H3K27ac. Enhancers globally retaining mitotic H3K4me1 or locally retaining mitotic
    H3K27ac are associated with cell type-specific genes and their transcription factors
    for rapid transcriptional activation. As cells exit mitosis, promoters regain
    H3K27ac, which correlates with transcriptional reactivation. Insulators also gain
    H3K27ac and CCCTC-binding factor (CTCF) in anaphase/telophase. This increase of
    H3K27ac in anaphase/telophase is required for posttranscriptional activation and
    may play a role in the establishment of topologically associating domains (TADs).
    Together, our results suggest that the genome is reorganized in a sequential order,
    in which histone methylations occur first in prometaphase, histone acetylation,
    and CTCF in anaphase/telophase, transcription in cytokinesis, and long-range chromatin
    interactions in early G1. We thus provide insights into the histone modification
    landscape that allows faithful reestablishment of the transcriptional program
    and TADs during cell division.
article_processing_charge: No
article_type: original
author:
- first_name: Hyeseon
  full_name: Kang, Hyeseon
  last_name: Kang
- first_name: Maxim N.
  full_name: Shokhirev, Maxim N.
  last_name: Shokhirev
- first_name: Zhichao
  full_name: Xu, Zhichao
  last_name: Xu
- first_name: Sahaana
  full_name: Chandran, Sahaana
  last_name: Chandran
- first_name: Jesse R.
  full_name: Dixon, Jesse R.
  last_name: Dixon
- first_name: Martin W
  full_name: HETZER, Martin W
  id: 86c0d31b-b4eb-11ec-ac5a-eae7b2e135ed
  last_name: HETZER
  orcid: 0000-0002-2111-992X
citation:
  ama: Kang H, Shokhirev MN, Xu Z, Chandran S, Dixon JR, Hetzer M. Dynamic regulation
    of histone modifications and long-range chromosomal interactions during postmitotic
    transcriptional reactivation. <i>Genes &#38; Development</i>. 2020;34(13-14):913-930.
    doi:<a href="https://doi.org/10.1101/gad.335794.119">10.1101/gad.335794.119</a>
  apa: Kang, H., Shokhirev, M. N., Xu, Z., Chandran, S., Dixon, J. R., &#38; Hetzer,
    M. (2020). Dynamic regulation of histone modifications and long-range chromosomal
    interactions during postmitotic transcriptional reactivation. <i>Genes &#38; Development</i>.
    Cold Spring Harbor Laboratory Press. <a href="https://doi.org/10.1101/gad.335794.119">https://doi.org/10.1101/gad.335794.119</a>
  chicago: Kang, Hyeseon, Maxim N. Shokhirev, Zhichao Xu, Sahaana Chandran, Jesse
    R. Dixon, and Martin Hetzer. “Dynamic Regulation of Histone Modifications and
    Long-Range Chromosomal Interactions during Postmitotic Transcriptional Reactivation.”
    <i>Genes &#38; Development</i>. Cold Spring Harbor Laboratory Press, 2020. <a
    href="https://doi.org/10.1101/gad.335794.119">https://doi.org/10.1101/gad.335794.119</a>.
  ieee: H. Kang, M. N. Shokhirev, Z. Xu, S. Chandran, J. R. Dixon, and M. Hetzer,
    “Dynamic regulation of histone modifications and long-range chromosomal interactions
    during postmitotic transcriptional reactivation,” <i>Genes &#38; Development</i>,
    vol. 34, no. 13–14. Cold Spring Harbor Laboratory Press, pp. 913–930, 2020.
  ista: Kang H, Shokhirev MN, Xu Z, Chandran S, Dixon JR, Hetzer M. 2020. Dynamic
    regulation of histone modifications and long-range chromosomal interactions during
    postmitotic transcriptional reactivation. Genes &#38; Development. 34(13–14),
    913–930.
  mla: Kang, Hyeseon, et al. “Dynamic Regulation of Histone Modifications and Long-Range
    Chromosomal Interactions during Postmitotic Transcriptional Reactivation.” <i>Genes
    &#38; Development</i>, vol. 34, no. 13–14, Cold Spring Harbor Laboratory Press,
    2020, pp. 913–30, doi:<a href="https://doi.org/10.1101/gad.335794.119">10.1101/gad.335794.119</a>.
  short: H. Kang, M.N. Shokhirev, Z. Xu, S. Chandran, J.R. Dixon, M. Hetzer, Genes
    &#38; Development 34 (2020) 913–930.
date_created: 2022-04-07T07:44:09Z
date_published: 2020-04-28T00:00:00Z
date_updated: 2022-07-18T08:31:08Z
day: '28'
ddc:
- '570'
doi: 10.1101/gad.335794.119
extern: '1'
external_id:
  pmid:
  - '32499403'
file:
- access_level: open_access
  checksum: 84e92d40e67936c739628315c238daf9
  content_type: application/pdf
  creator: dernst
  date_created: 2022-04-08T07:12:33Z
  date_updated: 2022-04-08T07:12:33Z
  file_id: '11136'
  file_name: 2020_GenesDevelopment_Kang.pdf
  file_size: 4406772
  relation: main_file
  success: 1
file_date_updated: 2022-04-08T07:12:33Z
has_accepted_license: '1'
intvolume: '        34'
issue: 13-14
keyword:
- Developmental Biology
- Genetics
language:
- iso: eng
license: https://creativecommons.org/licenses/by/4.0/
month: '04'
oa: 1
oa_version: Published Version
page: 913-930
pmid: 1
publication: Genes & Development
publication_identifier:
  issn:
  - 0890-9369
  - 1549-5477
publication_status: published
publisher: Cold Spring Harbor Laboratory Press
quality_controlled: '1'
scopus_import: '1'
status: public
title: Dynamic regulation of histone modifications and long-range chromosomal interactions
  during postmitotic transcriptional reactivation
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 72615eeb-f1f3-11ec-aa25-d4573ddc34fd
volume: 34
year: '2020'
...
---
_id: '11063'
abstract:
- lang: eng
  text: The total number of nuclear pore complexes (NPCs) per nucleus varies greatly
    between different cell types and is known to change during cell differentiation
    and cell transformation. However, the underlying mechanisms that control how many
    nuclear transport channels are assembled into a given nuclear envelope remain
    unclear. Here, we report that depletion of the NPC basket protein Tpr, but not
    Nup153, dramatically increases the total NPC number in various cell types. This
    negative regulation of Tpr occurs via a phosphorylation cascade of extracellular
    signal-regulated kinase (ERK), the central kinase of the mitogen-activated protein
    kinase (MAPK) pathway. Tpr serves as a scaffold for ERK to phosphorylate the nucleoporin
    (Nup) Nup153, which is critical for early stages of NPC biogenesis. Our results
    reveal a critical role of the Nup Tpr in coordinating signal transduction pathways
    during cell proliferation and the dynamic organization of the nucleus.
article_processing_charge: No
article_type: original
author:
- first_name: Asako
  full_name: McCloskey, Asako
  last_name: McCloskey
- first_name: Arkaitz
  full_name: Ibarra, Arkaitz
  last_name: Ibarra
- first_name: Martin W
  full_name: HETZER, Martin W
  id: 86c0d31b-b4eb-11ec-ac5a-eae7b2e135ed
  last_name: HETZER
  orcid: 0000-0002-2111-992X
citation:
  ama: McCloskey A, Ibarra A, Hetzer M. Tpr regulates the total number of nuclear
    pore complexes per cell nucleus. <i>Genes &#38; Development</i>. 2018;32(19-20):1321-1331.
    doi:<a href="https://doi.org/10.1101/gad.315523.118">10.1101/gad.315523.118</a>
  apa: McCloskey, A., Ibarra, A., &#38; Hetzer, M. (2018). Tpr regulates the total
    number of nuclear pore complexes per cell nucleus. <i>Genes &#38; Development</i>.
    Cold Spring Harbor Laboratory. <a href="https://doi.org/10.1101/gad.315523.118">https://doi.org/10.1101/gad.315523.118</a>
  chicago: McCloskey, Asako, Arkaitz Ibarra, and Martin Hetzer. “Tpr Regulates the
    Total Number of Nuclear Pore Complexes per Cell Nucleus.” <i>Genes &#38; Development</i>.
    Cold Spring Harbor Laboratory, 2018. <a href="https://doi.org/10.1101/gad.315523.118">https://doi.org/10.1101/gad.315523.118</a>.
  ieee: A. McCloskey, A. Ibarra, and M. Hetzer, “Tpr regulates the total number of
    nuclear pore complexes per cell nucleus,” <i>Genes &#38; Development</i>, vol.
    32, no. 19–20. Cold Spring Harbor Laboratory, pp. 1321–1331, 2018.
  ista: McCloskey A, Ibarra A, Hetzer M. 2018. Tpr regulates the total number of nuclear
    pore complexes per cell nucleus. Genes &#38; Development. 32(19–20), 1321–1331.
  mla: McCloskey, Asako, et al. “Tpr Regulates the Total Number of Nuclear Pore Complexes
    per Cell Nucleus.” <i>Genes &#38; Development</i>, vol. 32, no. 19–20, Cold Spring
    Harbor Laboratory, 2018, pp. 1321–31, doi:<a href="https://doi.org/10.1101/gad.315523.118">10.1101/gad.315523.118</a>.
  short: A. McCloskey, A. Ibarra, M. Hetzer, Genes &#38; Development 32 (2018) 1321–1331.
date_created: 2022-04-07T07:45:30Z
date_published: 2018-09-18T00:00:00Z
date_updated: 2022-07-18T08:32:32Z
day: '18'
doi: 10.1101/gad.315523.118
extern: '1'
external_id:
  pmid:
  - '30228202'
intvolume: '        32'
issue: 19-20
keyword:
- Developmental Biology
- Genetics
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: https://doi.org/10.1101/gad.315523.118
month: '09'
oa: 1
oa_version: Published Version
page: 1321-1331
pmid: 1
publication: Genes & Development
publication_identifier:
  issn:
  - 0890-9369
  - 1549-5477
publication_status: published
publisher: Cold Spring Harbor Laboratory
quality_controlled: '1'
scopus_import: '1'
status: public
title: Tpr regulates the total number of nuclear pore complexes per cell nucleus
type: journal_article
user_id: 72615eeb-f1f3-11ec-aa25-d4573ddc34fd
volume: 32
year: '2018'
...
---
_id: '11066'
abstract:
- lang: eng
  text: Recent studies have shown that a subset of nucleoporins (Nups) can detach
    from the nuclear pore complex and move into the nuclear interior to regulate transcription.
    One such dynamic Nup, called Nup98, has been implicated in gene activation in
    healthy cells and has been shown to drive leukemogenesis when mutated in patients
    with acute myeloid leukemia (AML). Here we show that in hematopoietic cells, Nup98
    binds predominantly to transcription start sites to recruit the Wdr82–Set1A/COMPASS
    (complex of proteins associated with Set1) complex, which is required for deposition
    of the histone 3 Lys4 trimethyl (H3K4me3)-activating mark. Depletion of Nup98
    or Wdr82 abolishes Set1A recruitment to chromatin and subsequently ablates H3K4me3
    at adjacent promoters. Furthermore, expression of a Nup98 fusion protein implicated
    in aggressive AML causes mislocalization of H3K4me3 at abnormal regions and up-regulation
    of associated genes. Our findings establish a function of Nup98 in hematopoietic
    gene activation and provide mechanistic insight into which Nup98 leukemic fusion
    proteins promote AML.
article_processing_charge: No
article_type: original
author:
- first_name: Tobias M.
  full_name: Franks, Tobias M.
  last_name: Franks
- first_name: Asako
  full_name: McCloskey, Asako
  last_name: McCloskey
- first_name: Maxim Nikolaievich
  full_name: Shokhirev, Maxim Nikolaievich
  last_name: Shokhirev
- first_name: Chris
  full_name: Benner, Chris
  last_name: Benner
- first_name: Annie
  full_name: Rathore, Annie
  last_name: Rathore
- first_name: Martin W
  full_name: HETZER, Martin W
  id: 86c0d31b-b4eb-11ec-ac5a-eae7b2e135ed
  last_name: HETZER
  orcid: 0000-0002-2111-992X
citation:
  ama: Franks TM, McCloskey A, Shokhirev MN, Benner C, Rathore A, Hetzer M. Nup98
    recruits the Wdr82–Set1A/COMPASS complex to promoters to regulate H3K4 trimethylation
    in hematopoietic progenitor cells. <i>Genes &#38; Development</i>. 2017;31(22):2222-2234.
    doi:<a href="https://doi.org/10.1101/gad.306753.117">10.1101/gad.306753.117</a>
  apa: Franks, T. M., McCloskey, A., Shokhirev, M. N., Benner, C., Rathore, A., &#38;
    Hetzer, M. (2017). Nup98 recruits the Wdr82–Set1A/COMPASS complex to promoters
    to regulate H3K4 trimethylation in hematopoietic progenitor cells. <i>Genes &#38;
    Development</i>. Cold Spring Harbor Laboratory. <a href="https://doi.org/10.1101/gad.306753.117">https://doi.org/10.1101/gad.306753.117</a>
  chicago: Franks, Tobias M., Asako McCloskey, Maxim Nikolaievich Shokhirev, Chris
    Benner, Annie Rathore, and Martin Hetzer. “Nup98 Recruits the Wdr82–Set1A/COMPASS
    Complex to Promoters to Regulate H3K4 Trimethylation in Hematopoietic Progenitor
    Cells.” <i>Genes &#38; Development</i>. Cold Spring Harbor Laboratory, 2017. <a
    href="https://doi.org/10.1101/gad.306753.117">https://doi.org/10.1101/gad.306753.117</a>.
  ieee: T. M. Franks, A. McCloskey, M. N. Shokhirev, C. Benner, A. Rathore, and M.
    Hetzer, “Nup98 recruits the Wdr82–Set1A/COMPASS complex to promoters to regulate
    H3K4 trimethylation in hematopoietic progenitor cells,” <i>Genes &#38; Development</i>,
    vol. 31, no. 22. Cold Spring Harbor Laboratory, pp. 2222–2234, 2017.
  ista: Franks TM, McCloskey A, Shokhirev MN, Benner C, Rathore A, Hetzer M. 2017.
    Nup98 recruits the Wdr82–Set1A/COMPASS complex to promoters to regulate H3K4 trimethylation
    in hematopoietic progenitor cells. Genes &#38; Development. 31(22), 2222–2234.
  mla: Franks, Tobias M., et al. “Nup98 Recruits the Wdr82–Set1A/COMPASS Complex to
    Promoters to Regulate H3K4 Trimethylation in Hematopoietic Progenitor Cells.”
    <i>Genes &#38; Development</i>, vol. 31, no. 22, Cold Spring Harbor Laboratory,
    2017, pp. 2222–34, doi:<a href="https://doi.org/10.1101/gad.306753.117">10.1101/gad.306753.117</a>.
  short: T.M. Franks, A. McCloskey, M.N. Shokhirev, C. Benner, A. Rathore, M. Hetzer,
    Genes &#38; Development 31 (2017) 2222–2234.
date_created: 2022-04-07T07:45:59Z
date_published: 2017-12-21T00:00:00Z
date_updated: 2022-07-18T08:33:05Z
day: '21'
doi: 10.1101/gad.306753.117
extern: '1'
external_id:
  pmid:
  - '29269482'
intvolume: '        31'
issue: '22'
keyword:
- Developmental Biology
- Genetics
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: https://doi.org/10.1101/gad.306753.117
month: '12'
oa: 1
oa_version: Published Version
page: 2222-2234
pmid: 1
publication: Genes & Development
publication_identifier:
  issn:
  - 0890-9369
  - 1549-5477
publication_status: published
publisher: Cold Spring Harbor Laboratory
quality_controlled: '1'
scopus_import: '1'
status: public
title: Nup98 recruits the Wdr82–Set1A/COMPASS complex to promoters to regulate H3K4
  trimethylation in hematopoietic progenitor cells
type: journal_article
user_id: 72615eeb-f1f3-11ec-aa25-d4573ddc34fd
volume: 31
year: '2017'
...