---
_id: '10614'
abstract:
- lang: eng
  text: 'The infiltration of immune cells into tissues underlies the establishment
    of tissue-resident macrophages and responses to infections and tumors. Yet the
    mechanisms immune cells utilize to negotiate tissue barriers in living organisms
    are not well understood, and a role for cortical actin has not been examined.
    Here, we find that the tissue invasion of Drosophila macrophages, also known as
    plasmatocytes or hemocytes, utilizes enhanced cortical F-actin levels stimulated
    by the Drosophila member of the fos proto oncogene transcription factor family
    (Dfos, Kayak). RNA sequencing analysis and live imaging show that Dfos enhances
    F-actin levels around the entire macrophage surface by increasing mRNA levels
    of the membrane spanning molecular scaffold tetraspanin TM4SF, and the actin cross-linking
    filamin Cheerio, which are themselves required for invasion. Both the filamin
    and the tetraspanin enhance the cortical activity of Rho1 and the formin Diaphanous
    and thus the assembly of cortical actin, which is a critical function since expressing
    a dominant active form of Diaphanous can rescue the Dfos macrophage invasion defect.
    In vivo imaging shows that Dfos enhances the efficiency of the initial phases
    of macrophage tissue entry. Genetic evidence argues that this Dfos-induced program
    in macrophages counteracts the constraint produced by the tension of surrounding
    tissues and buffers the properties of the macrophage nucleus from affecting tissue
    entry. We thus identify strengthening the cortical actin cytoskeleton through
    Dfos as a key process allowing efficient forward movement of an immune cell into
    surrounding tissues. '
acknowledged_ssus:
- _id: LifeSc
acknowledgement: 'We thank the following for their contributions: Plasmids were supplied
  by the Drosophila Genomics Resource Center (NIH 2P40OD010949-10A1); fly stocks were
  provided by K. Brueckner, B. Stramer, M. Uhlirova, O. Schuldiner, the Bloomington
  Drosophila Stock Center (NIH P40OD018537) and the Vienna Drosophila Resource Center,
  FlyBase for essential genomic information, and the BDGP in situ database for data.
  For antibodies, we thank the Developmental Studies Hybridoma Bank, which was created
  by the Eunice Kennedy Shriver National Institute of Child Health and Human Development
  of the NIH and is maintained at the University of Iowa, as well as J. Zeitlinger
  for her generous gift of Dfos antibody. We thank the Vienna BioCenter Core Facilities
  for RNA sequencing and analysis and the Life Scientific Service Units at IST Austria
  for technical support and assistance with microscopy and FACS analysis. We thank
  C. P. Heisenberg, P. Martin, M. Sixt, and Siekhaus group members for discussions
  and T. Hurd, A. Ratheesh, and P. Rangan for comments on the manuscript.'
article_processing_charge: No
article_type: original
author:
- first_name: Vera
  full_name: Belyaeva, Vera
  id: 47F080FE-F248-11E8-B48F-1D18A9856A87
  last_name: Belyaeva
- first_name: Stephanie
  full_name: Wachner, Stephanie
  id: 2A95E7B0-F248-11E8-B48F-1D18A9856A87
  last_name: Wachner
- first_name: Attila
  full_name: György, Attila
  id: 3BCEDBE0-F248-11E8-B48F-1D18A9856A87
  last_name: György
  orcid: 0000-0002-1819-198X
- first_name: Shamsi
  full_name: Emtenani, Shamsi
  id: 49D32318-F248-11E8-B48F-1D18A9856A87
  last_name: Emtenani
  orcid: 0000-0001-6981-6938
- first_name: Igor
  full_name: Gridchyn, Igor
  id: 4B60654C-F248-11E8-B48F-1D18A9856A87
  last_name: Gridchyn
  orcid: 0000-0002-1807-1929
- first_name: Maria
  full_name: Akhmanova, Maria
  id: 3425EC26-F248-11E8-B48F-1D18A9856A87
  last_name: Akhmanova
  orcid: 0000-0003-1522-3162
- first_name: M
  full_name: Linder, M
  last_name: Linder
- first_name: Marko
  full_name: Roblek, Marko
  id: 3047D808-F248-11E8-B48F-1D18A9856A87
  last_name: Roblek
  orcid: 0000-0001-9588-1389
- first_name: M
  full_name: Sibilia, M
  last_name: Sibilia
- first_name: Daria E
  full_name: Siekhaus, Daria E
  id: 3D224B9E-F248-11E8-B48F-1D18A9856A87
  last_name: Siekhaus
  orcid: 0000-0001-8323-8353
citation:
  ama: Belyaeva V, Wachner S, György A, et al. Fos regulates macrophage infiltration
    against surrounding tissue resistance by a cortical actin-based mechanism in Drosophila.
    <i>PLoS Biology</i>. 2022;20(1):e3001494. doi:<a href="https://doi.org/10.1371/journal.pbio.3001494">10.1371/journal.pbio.3001494</a>
  apa: Belyaeva, V., Wachner, S., György, A., Emtenani, S., Gridchyn, I., Akhmanova,
    M., … Siekhaus, D. E. (2022). Fos regulates macrophage infiltration against surrounding
    tissue resistance by a cortical actin-based mechanism in Drosophila. <i>PLoS Biology</i>.
    Public Library of Science. <a href="https://doi.org/10.1371/journal.pbio.3001494">https://doi.org/10.1371/journal.pbio.3001494</a>
  chicago: Belyaeva, Vera, Stephanie Wachner, Attila György, Shamsi Emtenani, Igor
    Gridchyn, Maria Akhmanova, M Linder, Marko Roblek, M Sibilia, and Daria E Siekhaus.
    “Fos Regulates Macrophage Infiltration against Surrounding Tissue Resistance by
    a Cortical Actin-Based Mechanism in Drosophila.” <i>PLoS Biology</i>. Public Library
    of Science, 2022. <a href="https://doi.org/10.1371/journal.pbio.3001494">https://doi.org/10.1371/journal.pbio.3001494</a>.
  ieee: V. Belyaeva <i>et al.</i>, “Fos regulates macrophage infiltration against
    surrounding tissue resistance by a cortical actin-based mechanism in Drosophila,”
    <i>PLoS Biology</i>, vol. 20, no. 1. Public Library of Science, p. e3001494, 2022.
  ista: Belyaeva V, Wachner S, György A, Emtenani S, Gridchyn I, Akhmanova M, Linder
    M, Roblek M, Sibilia M, Siekhaus DE. 2022. Fos regulates macrophage infiltration
    against surrounding tissue resistance by a cortical actin-based mechanism in Drosophila.
    PLoS Biology. 20(1), e3001494.
  mla: Belyaeva, Vera, et al. “Fos Regulates Macrophage Infiltration against Surrounding
    Tissue Resistance by a Cortical Actin-Based Mechanism in Drosophila.” <i>PLoS
    Biology</i>, vol. 20, no. 1, Public Library of Science, 2022, p. e3001494, doi:<a
    href="https://doi.org/10.1371/journal.pbio.3001494">10.1371/journal.pbio.3001494</a>.
  short: V. Belyaeva, S. Wachner, A. György, S. Emtenani, I. Gridchyn, M. Akhmanova,
    M. Linder, M. Roblek, M. Sibilia, D.E. Siekhaus, PLoS Biology 20 (2022) e3001494.
date_created: 2022-01-12T10:18:17Z
date_published: 2022-01-06T00:00:00Z
date_updated: 2024-03-25T23:30:15Z
day: '06'
ddc:
- '570'
department:
- _id: DaSi
- _id: JoCs
doi: 10.1371/journal.pbio.3001494
ec_funded: 1
external_id:
  isi:
  - '000971223700001'
  pmid:
  - '34990456'
file:
- access_level: open_access
  checksum: f454212a5522a7818ba4b2892315c478
  content_type: application/pdf
  creator: cchlebak
  date_created: 2022-01-12T13:50:04Z
  date_updated: 2022-01-12T13:50:04Z
  file_id: '10615'
  file_name: 2022_PLOSBio_Belyaeva.pdf
  file_size: 5426932
  relation: main_file
  success: 1
file_date_updated: 2022-01-12T13:50:04Z
has_accepted_license: '1'
intvolume: '        20'
isi: 1
issue: '1'
language:
- iso: eng
month: '01'
oa: 1
oa_version: Published Version
page: e3001494
pmid: 1
project:
- _id: 253B6E48-B435-11E9-9278-68D0E5697425
  call_identifier: FWF
  grant_number: P29638
  name: Drosophila TNFa´s Funktion in Immunzellen
- _id: 26199CA4-B435-11E9-9278-68D0E5697425
  grant_number: '24800'
  name: Tissue barrier penetration is crucial for immunity and metastasis
- _id: 2536F660-B435-11E9-9278-68D0E5697425
  call_identifier: FP7
  grant_number: '334077'
  name: Investigating the role of transporters in invasive migration through junctions
publication: PLoS Biology
publication_identifier:
  eissn:
  - 1545-7885
  issn:
  - 1544-9173
publication_status: published
publisher: Public Library of Science
quality_controlled: '1'
related_material:
  link:
  - relation: earlier_version
    url: https://www.biorxiv.org/content/10.1101/2020.09.18.301481
  - description: News on the ISTA Website
    relation: press_release
    url: https://ista.ac.at/en/news/resisting-the-pressure/
  record:
  - id: '8557'
    relation: earlier_version
    status: public
  - id: '11193'
    relation: dissertation_contains
    status: public
scopus_import: '1'
status: public
title: Fos regulates macrophage infiltration against surrounding tissue resistance
  by a cortical actin-based mechanism in Drosophila
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 20
year: '2022'
...
---
_id: '10322'
abstract:
- lang: eng
  text: To survive elevated temperatures, ectotherms adjust the fluidity of membranes
    by fine-tuning lipid desaturation levels in a process previously described to
    be cell autonomous. We have discovered that, in Caenorhabditis elegans, neuronal
    heat shock factor 1 (HSF-1), the conserved master regulator of the heat shock
    response (HSR), causes extensive fat remodeling in peripheral tissues. These changes
    include a decrease in fat desaturase and acid lipase expression in the intestine
    and a global shift in the saturation levels of plasma membrane’s phospholipids.
    The observed remodeling of plasma membrane is in line with ectothermic adaptive
    responses and gives worms a cumulative advantage to warm temperatures. We have
    determined that at least 6 TAX-2/TAX-4 cyclic guanosine monophosphate (cGMP) gated
    channel expressing sensory neurons, and transforming growth factor ß (TGF-β)/bone
    morphogenetic protein (BMP) are required for signaling across tissues to modulate
    fat desaturation. We also find neuronal hsf-1 is not only sufficient but also
    partially necessary to control the fat remodeling response and for survival at
    warm temperatures. This is the first study to show that a thermostat-based mechanism
    can cell nonautonomously coordinate membrane saturation and composition across
    tissues in a multicellular animal.
acknowledgement: We dedicate this work to the memory of Michael J.O. Wakelam. We would
  like to acknowledge Michael Fasseas (Invermis, Magnitude Biosciences) for plasmid
  injections and Sunny Biotech for transgenics; Catalina Vallejos and John Marioni
  for statistical advice at the beginning of the work; Simon Walker, Imaging, Bioinformatics
  and Lipidomics Facilities at Babraham Institute for technical support; and Cindy
  Voisine, Michael Witting, Jon Houseley, Len Stephens, Carmen Nussbaum Krammer, Rebeca
  Aldunate, Patricija van Oosten-Hawle, Jean-Louis Bessereau, and Jane Alfred for
  feedback on the manuscript. We thank Andy Dillin, Atsushi Kuhara, Amy Walker, Andrew
  Leifer, Yun Zhang, and Michalis Barkoulas for reagents and Julie Ahringer, Anne
  Ferguson-Smith, and Anne Corcoran for support and helpful discussions. We also acknowledge
  Babraham Institute Facilities.
article_number: e3001431
article_processing_charge: No
article_type: original
author:
- first_name: Laetitia
  full_name: Chauve, Laetitia
  last_name: Chauve
- first_name: Francesca
  full_name: Hodge, Francesca
  last_name: Hodge
- first_name: Sharlene
  full_name: Murdoch, Sharlene
  last_name: Murdoch
- first_name: Fatemah
  full_name: Masoudzadeh, Fatemah
  last_name: Masoudzadeh
- first_name: Harry Jack
  full_name: Mann, Harry Jack
  last_name: Mann
- first_name: Andrea
  full_name: Lopez-Clavijo, Andrea
  last_name: Lopez-Clavijo
- first_name: Hanneke
  full_name: Okkenhaug, Hanneke
  last_name: Okkenhaug
- first_name: Greg
  full_name: West, Greg
  last_name: West
- first_name: Bebiana C.
  full_name: Sousa, Bebiana C.
  last_name: Sousa
- first_name: Anne
  full_name: Segonds-Pichon, Anne
  last_name: Segonds-Pichon
- first_name: Cheryl
  full_name: Li, Cheryl
  last_name: Li
- first_name: Steven
  full_name: Wingett, Steven
  last_name: Wingett
- first_name: Hermine
  full_name: Kienberger, Hermine
  last_name: Kienberger
- first_name: Karin
  full_name: Kleigrewe, Karin
  last_name: Kleigrewe
- first_name: Mario
  full_name: De Bono, Mario
  id: 4E3FF80E-F248-11E8-B48F-1D18A9856A87
  last_name: De Bono
  orcid: 0000-0001-8347-0443
- first_name: Michael
  full_name: Wakelam, Michael
  last_name: Wakelam
- first_name: Olivia
  full_name: Casanueva, Olivia
  last_name: Casanueva
citation:
  ama: Chauve L, Hodge F, Murdoch S, et al. Neuronal HSF-1 coordinates the propagation
    of fat desaturation across tissues to enable adaptation to high temperatures in
    C. elegans. <i>PLoS Biology</i>. 2021;19(11). doi:<a href="https://doi.org/10.1371/journal.pbio.3001431">10.1371/journal.pbio.3001431</a>
  apa: Chauve, L., Hodge, F., Murdoch, S., Masoudzadeh, F., Mann, H. J., Lopez-Clavijo,
    A., … Casanueva, O. (2021). Neuronal HSF-1 coordinates the propagation of fat
    desaturation across tissues to enable adaptation to high temperatures in C. elegans.
    <i>PLoS Biology</i>. Public Library of Science. <a href="https://doi.org/10.1371/journal.pbio.3001431">https://doi.org/10.1371/journal.pbio.3001431</a>
  chicago: Chauve, Laetitia, Francesca Hodge, Sharlene Murdoch, Fatemah Masoudzadeh,
    Harry Jack Mann, Andrea Lopez-Clavijo, Hanneke Okkenhaug, et al. “Neuronal HSF-1
    Coordinates the Propagation of Fat Desaturation across Tissues to Enable Adaptation
    to High Temperatures in C. Elegans.” <i>PLoS Biology</i>. Public Library of Science,
    2021. <a href="https://doi.org/10.1371/journal.pbio.3001431">https://doi.org/10.1371/journal.pbio.3001431</a>.
  ieee: L. Chauve <i>et al.</i>, “Neuronal HSF-1 coordinates the propagation of fat
    desaturation across tissues to enable adaptation to high temperatures in C. elegans,”
    <i>PLoS Biology</i>, vol. 19, no. 11. Public Library of Science, 2021.
  ista: Chauve L, Hodge F, Murdoch S, Masoudzadeh F, Mann HJ, Lopez-Clavijo A, Okkenhaug
    H, West G, Sousa BC, Segonds-Pichon A, Li C, Wingett S, Kienberger H, Kleigrewe
    K, de Bono M, Wakelam M, Casanueva O. 2021. Neuronal HSF-1 coordinates the propagation
    of fat desaturation across tissues to enable adaptation to high temperatures in
    C. elegans. PLoS Biology. 19(11), e3001431.
  mla: Chauve, Laetitia, et al. “Neuronal HSF-1 Coordinates the Propagation of Fat
    Desaturation across Tissues to Enable Adaptation to High Temperatures in C. Elegans.”
    <i>PLoS Biology</i>, vol. 19, no. 11, e3001431, Public Library of Science, 2021,
    doi:<a href="https://doi.org/10.1371/journal.pbio.3001431">10.1371/journal.pbio.3001431</a>.
  short: L. Chauve, F. Hodge, S. Murdoch, F. Masoudzadeh, H.J. Mann, A. Lopez-Clavijo,
    H. Okkenhaug, G. West, B.C. Sousa, A. Segonds-Pichon, C. Li, S. Wingett, H. Kienberger,
    K. Kleigrewe, M. de Bono, M. Wakelam, O. Casanueva, PLoS Biology 19 (2021).
date_created: 2021-11-21T23:01:28Z
date_published: 2021-11-01T00:00:00Z
date_updated: 2023-08-14T11:53:27Z
day: '01'
ddc:
- '570'
department:
- _id: MaDe
doi: 10.1371/journal.pbio.3001431
external_id:
  isi:
  - '000715818400001'
  pmid:
  - '34723964'
file:
- access_level: open_access
  checksum: 0c61b667f814fd9435b3ac42036fc36d
  content_type: application/pdf
  creator: cchlebak
  date_created: 2021-11-22T09:34:03Z
  date_updated: 2021-11-22T09:34:03Z
  file_id: '10330'
  file_name: 2021_PLoSBio_Chauve.pdf
  file_size: 4069215
  relation: main_file
  success: 1
file_date_updated: 2021-11-22T09:34:03Z
has_accepted_license: '1'
intvolume: '        19'
isi: 1
issue: '11'
language:
- iso: eng
month: '11'
oa: 1
oa_version: Published Version
pmid: 1
publication: PLoS Biology
publication_identifier:
  eissn:
  - 1545-7885
  issn:
  - 1544-9173
publication_status: published
publisher: Public Library of Science
quality_controlled: '1'
related_material:
  record:
  - id: '13069'
    relation: research_data
    status: public
scopus_import: '1'
status: public
title: Neuronal HSF-1 coordinates the propagation of fat desaturation across tissues
  to enable adaptation to high temperatures in C. elegans
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 19
year: '2021'
...
---
_id: '9517'
abstract:
- lang: eng
  text: Multicellular eukaryotes produce small RNA molecules (approximately 21–24
    nucleotides) of two general types, microRNA (miRNA) and short interfering RNA
    (siRNA). They collectively function as sequence-specific guides to silence or
    regulate genes, transposons, and viruses and to modify chromatin and genome structure.
    Formation or activity of small RNAs requires factors belonging to gene families
    that encode DICER (or DICER-LIKE [DCL]) and ARGONAUTE proteins and, in the case
    of some siRNAs, RNA-dependent RNA polymerase (RDR) proteins. Unlike many animals,
    plants encode multiple DCL and RDR proteins. Using a series of insertion mutants
    of Arabidopsis thaliana, unique functions for three DCL proteins in miRNA (DCL1),
    endogenous siRNA (DCL3), and viral siRNA (DCL2) biogenesis were identified. One
    RDR protein (RDR2) was required for all endogenous siRNAs analyzed. The loss of
    endogenous siRNA in dcl3 and rdr2 mutants was associated with loss of heterochromatic
    marks and increased transcript accumulation at some loci. Defects in siRNA-generation
    activity in response to turnip crinkle virus in dcl2 mutant plants correlated
    with increased virus susceptibility. We conclude that proliferation and diversification
    of DCL and RDR genes during evolution of plants contributed to specialization
    of small RNA-directed pathways for development, chromatin structure, and defense.
article_processing_charge: No
article_type: original
author:
- first_name: Zhixin
  full_name: Xie, Zhixin
  last_name: Xie
- first_name: Lisa K.
  full_name: Johansen, Lisa K.
  last_name: Johansen
- first_name: Adam M.
  full_name: Gustafson, Adam M.
  last_name: Gustafson
- first_name: Kristin D.
  full_name: Kasschau, Kristin D.
  last_name: Kasschau
- first_name: 'Andrew D. '
  full_name: 'Lellis, Andrew D. '
  last_name: Lellis
- first_name: Daniel
  full_name: Zilberman, Daniel
  id: 6973db13-dd5f-11ea-814e-b3e5455e9ed1
  last_name: Zilberman
  orcid: 0000-0002-0123-8649
- first_name: Steven E.
  full_name: Jacobsen, Steven E.
  last_name: Jacobsen
- first_name: James C.
  full_name: Carrington, James C.
  last_name: Carrington
citation:
  ama: Xie Z, Johansen LK, Gustafson AM, et al. Genetic and functional diversification
    of small RNA pathways in plants. <i>PLoS Biology</i>. 2004;2(5):0642-0652. doi:<a
    href="https://doi.org/10.1371/journal.pbio.0020104">10.1371/journal.pbio.0020104</a>
  apa: Xie, Z., Johansen, L. K., Gustafson, A. M., Kasschau, K. D., Lellis, A. D.,
    Zilberman, D., … Carrington, J. C. (2004). Genetic and functional diversification
    of small RNA pathways in plants. <i>PLoS Biology</i>. Public Library of Science.
    <a href="https://doi.org/10.1371/journal.pbio.0020104">https://doi.org/10.1371/journal.pbio.0020104</a>
  chicago: Xie, Zhixin, Lisa K. Johansen, Adam M. Gustafson, Kristin D. Kasschau,
    Andrew D.  Lellis, Daniel Zilberman, Steven E. Jacobsen, and James C. Carrington.
    “Genetic and Functional Diversification of Small RNA Pathways in Plants.” <i>PLoS
    Biology</i>. Public Library of Science, 2004. <a href="https://doi.org/10.1371/journal.pbio.0020104">https://doi.org/10.1371/journal.pbio.0020104</a>.
  ieee: Z. Xie <i>et al.</i>, “Genetic and functional diversification of small RNA
    pathways in plants,” <i>PLoS Biology</i>, vol. 2, no. 5. Public Library of Science,
    pp. 0642–0652, 2004.
  ista: Xie Z, Johansen LK, Gustafson AM, Kasschau KD, Lellis AD, Zilberman D, Jacobsen
    SE, Carrington JC. 2004. Genetic and functional diversification of small RNA pathways
    in plants. PLoS Biology. 2(5), 0642–0652.
  mla: Xie, Zhixin, et al. “Genetic and Functional Diversification of Small RNA Pathways
    in Plants.” <i>PLoS Biology</i>, vol. 2, no. 5, Public Library of Science, 2004,
    pp. 0642–52, doi:<a href="https://doi.org/10.1371/journal.pbio.0020104">10.1371/journal.pbio.0020104</a>.
  short: Z. Xie, L.K. Johansen, A.M. Gustafson, K.D. Kasschau, A.D. Lellis, D. Zilberman,
    S.E. Jacobsen, J.C. Carrington, PLoS Biology 2 (2004) 0642–0652.
date_created: 2021-06-07T14:12:08Z
date_published: 2004-02-24T00:00:00Z
date_updated: 2021-12-14T08:43:57Z
day: '24'
department:
- _id: DaZi
doi: 10.1371/journal.pbio.0020104
extern: '1'
external_id:
  pmid:
  - '15024409'
intvolume: '         2'
issue: '5'
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: https://doi.org/10.1371/journal.pbio.0020104
month: '02'
oa: 1
oa_version: Published Version
page: 0642-0652
pmid: 1
publication: PLoS Biology
publication_identifier:
  eissn:
  - 1545-7885
  issn:
  - 1544-9173
publication_status: published
publisher: Public Library of Science
quality_controlled: '1'
scopus_import: '1'
status: public
title: Genetic and functional diversification of small RNA pathways in plants
type: journal_article
user_id: 8b945eb4-e2f2-11eb-945a-df72226e66a9
volume: 2
year: '2004'
...