---
_id: '6126'
abstract:
- lang: eng
text: Aerobic animals constantly monitor and adapt to changes in O2 levels. The
molecular mechanisms involved in sensing O2 are, however, incompletely understood.
Previous studies showed that a hexacoordinated globin called GLB-5 tunes the dynamic
range of O2-sensing neurons in natural C. elegans isolates, but is defective in
the N2 lab reference strain (McGrath et al., 2009; Persson et al., 2009). GLB-5
enables a sharp behavioral switch when O2 changes between 21 and 17%. Here, we
show that GLB-5 also confers rapid behavioral and cellular recovery from exposure
to hypoxia. Hypoxia reconfigures O2-evoked Ca2+ responses in the URX O2 sensors,
and GLB-5 enables rapid recovery of these responses upon re-oxygenation. Forward
genetic screens indicate that GLB-5's effects on O2 sensing require PDL-1, the
C. elegans ortholog of mammalian PrBP/PDE6δ protein. In mammals, PDE6δ regulates
the traffic and activity of prenylated proteins (Zhang et al., 2004; Norton et
al., 2005). PDL-1 promotes localization of GCY-33 and GCY-35, atypical soluble
guanylate cyclases that act as O2 sensors, to the dendritic endings of URX and
BAG neurons, where they colocalize with GLB-5. Both GCY-33 and GCY-35 are predicted
to be prenylated. Dendritic localization is not essential for GCY-35 to function
as an O2 sensor, but disrupting pdl-1 alters the URX neuron's O2 response properties.
Functional GLB-5 can restore dendritic localization of GCY-33 in pdl-1 mutants,
suggesting GCY-33 and GLB-5 are in a complex. Our data suggest GLB-5 and the soluble
guanylate cyclases operate in close proximity to sculpt O2 responses.
author:
- first_name: E.
full_name: Gross, E.
last_name: Gross
- first_name: Z.
full_name: Soltesz, Z.
last_name: Soltesz
- first_name: S.
full_name: Oda, S.
last_name: Oda
- first_name: V.
full_name: Zelmanovich, V.
last_name: Zelmanovich
- first_name: Z.
full_name: Abergel, Z.
last_name: Abergel
- first_name: Mario
full_name: de Bono, Mario
id: 4E3FF80E-F248-11E8-B48F-1D18A9856A87
last_name: de Bono
orcid: 0000-0001-8347-0443
citation:
ama: Gross E, Soltesz Z, Oda S, Zelmanovich V, Abergel Z, de Bono M. GLOBIN-5-dependent
O2 responses are regulated by PDL-1/PrBP that targets prenylated soluble guanylate
cyclases to dendritic endings. Journal of Neuroscience. 2014;34(50):16726-16738.
doi:10.1523/jneurosci.5368-13.2014
apa: Gross, E., Soltesz, Z., Oda, S., Zelmanovich, V., Abergel, Z., & de Bono,
M. (2014). GLOBIN-5-dependent O2 responses are regulated by PDL-1/PrBP that targets
prenylated soluble guanylate cyclases to dendritic endings. Journal of Neuroscience.
Society for Neuroscience. https://doi.org/10.1523/jneurosci.5368-13.2014
chicago: Gross, E., Z. Soltesz, S. Oda, V. Zelmanovich, Z. Abergel, and Mario de
Bono. “GLOBIN-5-Dependent O2 Responses Are Regulated by PDL-1/PrBP That Targets
Prenylated Soluble Guanylate Cyclases to Dendritic Endings.” Journal of Neuroscience.
Society for Neuroscience, 2014. https://doi.org/10.1523/jneurosci.5368-13.2014.
ieee: E. Gross, Z. Soltesz, S. Oda, V. Zelmanovich, Z. Abergel, and M. de Bono,
“GLOBIN-5-dependent O2 responses are regulated by PDL-1/PrBP that targets prenylated
soluble guanylate cyclases to dendritic endings,” Journal of Neuroscience,
vol. 34, no. 50. Society for Neuroscience, pp. 16726–16738, 2014.
ista: Gross E, Soltesz Z, Oda S, Zelmanovich V, Abergel Z, de Bono M. 2014. GLOBIN-5-dependent
O2 responses are regulated by PDL-1/PrBP that targets prenylated soluble guanylate
cyclases to dendritic endings. Journal of Neuroscience. 34(50), 16726–16738.
mla: Gross, E., et al. “GLOBIN-5-Dependent O2 Responses Are Regulated by PDL-1/PrBP
That Targets Prenylated Soluble Guanylate Cyclases to Dendritic Endings.” Journal
of Neuroscience, vol. 34, no. 50, Society for Neuroscience, 2014, pp. 16726–38,
doi:10.1523/jneurosci.5368-13.2014.
short: E. Gross, Z. Soltesz, S. Oda, V. Zelmanovich, Z. Abergel, M. de Bono, Journal
of Neuroscience 34 (2014) 16726–16738.
date_created: 2019-03-19T14:52:26Z
date_published: 2014-12-10T00:00:00Z
date_updated: 2021-01-12T08:06:14Z
day: '10'
ddc:
- '570'
doi: 10.1523/jneurosci.5368-13.2014
extern: '1'
external_id:
pmid:
- '25505325'
file:
- access_level: open_access
checksum: a3dd71969f94c43909327cd083283d4b
content_type: application/pdf
creator: kschuh
date_created: 2019-03-19T14:55:58Z
date_updated: 2020-07-14T12:47:20Z
file_id: '6127'
file_name: 2014_SFN_Gross.pdf
file_size: 3263422
relation: main_file
file_date_updated: 2020-07-14T12:47:20Z
has_accepted_license: '1'
intvolume: ' 34'
issue: '50'
language:
- iso: eng
month: '12'
oa: 1
oa_version: Published Version
page: 16726-16738
pmid: 1
publication: Journal of Neuroscience
publication_identifier:
issn:
- 0270-6474
- 1529-2401
publication_status: published
publisher: Society for Neuroscience
quality_controlled: '1'
status: public
title: GLOBIN-5-dependent O2 responses are regulated by PDL-1/PrBP that targets prenylated
soluble guanylate cyclases to dendritic endings
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 34
year: '2014'
...
---
_id: '8025'
abstract:
- lang: eng
text: Chandelier (axoaxonic) cells (ChCs) are a distinct group of GABAergic interneurons
that innervate the axon initial segments of pyramidal cells. However, their circuit
role and the function of their clearly defined anatomical specificity remain unclear.
Recent work has demonstrated that chandelier cells can produce depolarizing GABAergic
PSPs, occasionally driving postsynaptic targets to spike. On the other hand, other
work suggests that ChCs are hyperpolarizing and may have an inhibitory role. These
disparate functional effects may reflect heterogeneity among ChCs. Here, using
brain slices from transgenic mouse strains, we first demonstrate that, across
different neocortical areas and genetic backgrounds, upper Layer 2/3 ChCs belong
to a single electrophysiologically and morphologically defined population, extensively
sampling Layer 1 inputs with asymmetric dendrites. Consistent with being a single
cell type, we find electrical coupling between ChCs. We then investigate the effect
of chandelier cell activation on pyramidal neuron spiking in several conditions,
ranging from the resting membrane potential to stimuli designed to approximate
in vivo membrane potential dynamics. We find that under quiescent conditions,
chandelier cells are capable of both promoting and inhibiting spike generation,
depending on the postsynaptic membrane potential. However, during in vivo-like
membrane potential fluctuations, the dominant postsynaptic effect was a strong
inhibition. Thus, neocortical chandelier cells, even from within a homogeneous
population, appear to play a dual role in the circuit, helping to activate quiescent
pyramidal neurons, while at the same time inhibiting active ones.
article_processing_charge: No
article_type: original
author:
- first_name: A. R.
full_name: Woodruff, A. R.
last_name: Woodruff
- first_name: L. M.
full_name: McGarry, L. M.
last_name: McGarry
- first_name: Tim P
full_name: Vogels, Tim P
id: CB6FF8D2-008F-11EA-8E08-2637E6697425
last_name: Vogels
orcid: 0000-0003-3295-6181
- first_name: M.
full_name: Inan, M.
last_name: Inan
- first_name: S. A.
full_name: Anderson, S. A.
last_name: Anderson
- first_name: R.
full_name: Yuste, R.
last_name: Yuste
citation:
ama: Woodruff AR, McGarry LM, Vogels TP, Inan M, Anderson SA, Yuste R. State-dependent
function of neocortical chandelier cells. Journal of Neuroscience. 2011;31(49):17872-17886.
doi:10.1523/jneurosci.3894-11.2011
apa: Woodruff, A. R., McGarry, L. M., Vogels, T. P., Inan, M., Anderson, S. A.,
& Yuste, R. (2011). State-dependent function of neocortical chandelier cells.
Journal of Neuroscience. Society for Neuroscience. https://doi.org/10.1523/jneurosci.3894-11.2011
chicago: Woodruff, A. R., L. M. McGarry, Tim P Vogels, M. Inan, S. A. Anderson,
and R. Yuste. “State-Dependent Function of Neocortical Chandelier Cells.” Journal
of Neuroscience. Society for Neuroscience, 2011. https://doi.org/10.1523/jneurosci.3894-11.2011.
ieee: A. R. Woodruff, L. M. McGarry, T. P. Vogels, M. Inan, S. A. Anderson, and
R. Yuste, “State-dependent function of neocortical chandelier cells,” Journal
of Neuroscience, vol. 31, no. 49. Society for Neuroscience, pp. 17872–17886,
2011.
ista: Woodruff AR, McGarry LM, Vogels TP, Inan M, Anderson SA, Yuste R. 2011. State-dependent
function of neocortical chandelier cells. Journal of Neuroscience. 31(49), 17872–17886.
mla: Woodruff, A. R., et al. “State-Dependent Function of Neocortical Chandelier
Cells.” Journal of Neuroscience, vol. 31, no. 49, Society for Neuroscience,
2011, pp. 17872–86, doi:10.1523/jneurosci.3894-11.2011.
short: A.R. Woodruff, L.M. McGarry, T.P. Vogels, M. Inan, S.A. Anderson, R. Yuste,
Journal of Neuroscience 31 (2011) 17872–17886.
date_created: 2020-06-25T13:09:49Z
date_published: 2011-12-07T00:00:00Z
date_updated: 2021-01-12T08:16:36Z
day: '7'
doi: 10.1523/jneurosci.3894-11.2011
extern: '1'
external_id:
pmid:
- '22159102'
intvolume: ' 31'
issue: '49'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4071969/
month: '12'
oa: 1
oa_version: Published Version
page: 17872-17886
pmid: 1
publication: Journal of Neuroscience
publication_identifier:
issn:
- 0270-6474
- 1529-2401
publication_status: published
publisher: Society for Neuroscience
quality_controlled: '1'
status: public
title: State-dependent function of neocortical chandelier cells
type: journal_article
user_id: D865714E-FA4E-11E9-B85B-F5C5E5697425
volume: 31
year: '2011'
...
---
_id: '8028'
abstract:
- lang: eng
text: 'Transmission of signals within the brain is essential for cognitive function,
but it is not clear how neural circuits support reliable and accurate signal propagation
over a sufficiently large dynamic range. Two modes of propagation have been studied:
synfire chains, in which synchronous activity travels through feedforward layers
of a neuronal network, and the propagation of fluctuations in firing rate across
these layers. In both cases, a sufficient amount of noise, which was added to
previous models from an external source, had to be included to support stable
propagation. Sparse, randomly connected networks of spiking model neurons can
generate chaotic patterns of activity. We investigate whether this activity, which
is a more realistic noise source, is sufficient to allow for signal transmission.
We find that, for rate-coded signals but not for synfire chains, such networks
support robust and accurate signal reproduction through up to six layers if appropriate
adjustments are made in synaptic strengths. We investigate the factors affecting
transmission and show that multiple signals can propagate simultaneously along
different pathways. Using this feature, we show how different types of logic gates
can arise within the architecture of the random network through the strengthening
of specific synapses.'
article_processing_charge: No
article_type: original
author:
- first_name: Tim P
full_name: Vogels, Tim P
id: CB6FF8D2-008F-11EA-8E08-2637E6697425
last_name: Vogels
orcid: 0000-0003-3295-6181
- first_name: L. F.
full_name: Abbott, L. F.
last_name: Abbott
citation:
ama: Vogels TP, Abbott LF. Signal propagation and logic gating in networks of integrate-and-fire
neurons. Journal of Neuroscience. 2005;25(46):10786-10795. doi:10.1523/jneurosci.3508-05.2005
apa: Vogels, T. P., & Abbott, L. F. (2005). Signal propagation and logic gating
in networks of integrate-and-fire neurons. Journal of Neuroscience. Society
for Neuroscience. https://doi.org/10.1523/jneurosci.3508-05.2005
chicago: Vogels, Tim P, and L. F. Abbott. “Signal Propagation and Logic Gating in
Networks of Integrate-and-Fire Neurons.” Journal of Neuroscience. Society
for Neuroscience, 2005. https://doi.org/10.1523/jneurosci.3508-05.2005.
ieee: T. P. Vogels and L. F. Abbott, “Signal propagation and logic gating in networks
of integrate-and-fire neurons,” Journal of Neuroscience, vol. 25, no. 46.
Society for Neuroscience, pp. 10786–10795, 2005.
ista: Vogels TP, Abbott LF. 2005. Signal propagation and logic gating in networks
of integrate-and-fire neurons. Journal of Neuroscience. 25(46), 10786–10795.
mla: Vogels, Tim P., and L. F. Abbott. “Signal Propagation and Logic Gating in Networks
of Integrate-and-Fire Neurons.” Journal of Neuroscience, vol. 25, no. 46,
Society for Neuroscience, 2005, pp. 10786–95, doi:10.1523/jneurosci.3508-05.2005.
short: T.P. Vogels, L.F. Abbott, Journal of Neuroscience 25 (2005) 10786–10795.
date_created: 2020-06-25T13:12:33Z
date_published: 2005-11-16T00:00:00Z
date_updated: 2021-01-12T08:16:37Z
day: '16'
doi: 10.1523/jneurosci.3508-05.2005
extern: '1'
external_id:
pmid:
- '16291952'
intvolume: ' 25'
issue: '46'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6725859/
month: '11'
oa: 1
oa_version: Published Version
page: 10786-10795
pmid: 1
publication: Journal of Neuroscience
publication_identifier:
issn:
- 0270-6474
- 1529-2401
publication_status: published
publisher: Society for Neuroscience
quality_controlled: '1'
status: public
title: Signal propagation and logic gating in networks of integrate-and-fire neurons
type: journal_article
user_id: D865714E-FA4E-11E9-B85B-F5C5E5697425
volume: 25
year: '2005'
...