---
_id: '8442'
abstract:
- lang: eng
  text: Membrane proteins perform a host of vital cellular functions. Deciphering
    the molecular mechanisms whereby they fulfill these functions requires detailed
    biophysical and structural investigations. Detergents have proven pivotal to extract
    the protein from its native surroundings. Yet, they provide a milieu that departs
    significantly from that of the biological membrane, to the extent that the structure,
    the dynamics, and the interactions of membrane proteins in detergents may considerably
    vary, as compared to the native environment. Understanding the impact of detergents
    on membrane proteins is, therefore, crucial to assess the biological relevance
    of results obtained in detergents. Here, we review the strengths and weaknesses
    of alkyl phosphocholines (or foscholines), the most widely used detergent in solution-NMR
    studies of membrane proteins. While this class of detergents is often successful
    for membrane protein solubilization, a growing list of examples points to destabilizing
    and denaturing properties, in particular for α-helical membrane proteins. Our
    comprehensive analysis stresses the importance of stringent controls when working
    with this class of detergents and when analyzing the structure and dynamics of
    membrane proteins in alkyl phosphocholine detergents.
article_processing_charge: No
article_type: original
author:
- first_name: Christophe
  full_name: Chipot, Christophe
  last_name: Chipot
- first_name: François
  full_name: Dehez, François
  last_name: Dehez
- first_name: Jason R.
  full_name: Schnell, Jason R.
  last_name: Schnell
- first_name: Nicole
  full_name: Zitzmann, Nicole
  last_name: Zitzmann
- first_name: Eva
  full_name: Pebay-Peyroula, Eva
  last_name: Pebay-Peyroula
- first_name: Laurent J.
  full_name: Catoire, Laurent J.
  last_name: Catoire
- first_name: Bruno
  full_name: Miroux, Bruno
  last_name: Miroux
- first_name: Edmund R. S.
  full_name: Kunji, Edmund R. S.
  last_name: Kunji
- first_name: Gianluigi
  full_name: Veglia, Gianluigi
  last_name: Veglia
- first_name: Timothy A.
  full_name: Cross, Timothy A.
  last_name: Cross
- first_name: Paul
  full_name: Schanda, Paul
  id: 7B541462-FAF6-11E9-A490-E8DFE5697425
  last_name: Schanda
  orcid: 0000-0002-9350-7606
citation:
  ama: 'Chipot C, Dehez F, Schnell JR, et al. Perturbations of native membrane protein
    structure in alkyl phosphocholine detergents: A critical assessment of NMR and
    biophysical studies. <i>Chemical Reviews</i>. 2018;118(7):3559-3607. doi:<a href="https://doi.org/10.1021/acs.chemrev.7b00570">10.1021/acs.chemrev.7b00570</a>'
  apa: 'Chipot, C., Dehez, F., Schnell, J. R., Zitzmann, N., Pebay-Peyroula, E., Catoire,
    L. J., … Schanda, P. (2018). Perturbations of native membrane protein structure
    in alkyl phosphocholine detergents: A critical assessment of NMR and biophysical
    studies. <i>Chemical Reviews</i>. American Chemical Society. <a href="https://doi.org/10.1021/acs.chemrev.7b00570">https://doi.org/10.1021/acs.chemrev.7b00570</a>'
  chicago: 'Chipot, Christophe, François Dehez, Jason R. Schnell, Nicole Zitzmann,
    Eva Pebay-Peyroula, Laurent J. Catoire, Bruno Miroux, et al. “Perturbations of
    Native Membrane Protein Structure in Alkyl Phosphocholine Detergents: A Critical
    Assessment of NMR and Biophysical Studies.” <i>Chemical Reviews</i>. American
    Chemical Society, 2018. <a href="https://doi.org/10.1021/acs.chemrev.7b00570">https://doi.org/10.1021/acs.chemrev.7b00570</a>.'
  ieee: 'C. Chipot <i>et al.</i>, “Perturbations of native membrane protein structure
    in alkyl phosphocholine detergents: A critical assessment of NMR and biophysical
    studies,” <i>Chemical Reviews</i>, vol. 118, no. 7. American Chemical Society,
    pp. 3559–3607, 2018.'
  ista: 'Chipot C, Dehez F, Schnell JR, Zitzmann N, Pebay-Peyroula E, Catoire LJ,
    Miroux B, Kunji ERS, Veglia G, Cross TA, Schanda P. 2018. Perturbations of native
    membrane protein structure in alkyl phosphocholine detergents: A critical assessment
    of NMR and biophysical studies. Chemical Reviews. 118(7), 3559–3607.'
  mla: 'Chipot, Christophe, et al. “Perturbations of Native Membrane Protein Structure
    in Alkyl Phosphocholine Detergents: A Critical Assessment of NMR and Biophysical
    Studies.” <i>Chemical Reviews</i>, vol. 118, no. 7, American Chemical Society,
    2018, pp. 3559–607, doi:<a href="https://doi.org/10.1021/acs.chemrev.7b00570">10.1021/acs.chemrev.7b00570</a>.'
  short: C. Chipot, F. Dehez, J.R. Schnell, N. Zitzmann, E. Pebay-Peyroula, L.J. Catoire,
    B. Miroux, E.R.S. Kunji, G. Veglia, T.A. Cross, P. Schanda, Chemical Reviews 118
    (2018) 3559–3607.
date_created: 2020-09-18T10:05:35Z
date_published: 2018-02-28T00:00:00Z
date_updated: 2021-01-12T08:19:18Z
day: '28'
doi: 10.1021/acs.chemrev.7b00570
extern: '1'
intvolume: '       118'
issue: '7'
keyword:
- General Chemistry
language:
- iso: eng
month: '02'
oa_version: None
page: 3559-3607
publication: Chemical Reviews
publication_identifier:
  issn:
  - 0009-2665
  - 1520-6890
publication_status: published
publisher: American Chemical Society
quality_controlled: '1'
status: public
title: 'Perturbations of native membrane protein structure in alkyl phosphocholine
  detergents: A critical assessment of NMR and biophysical studies'
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 118
year: '2018'
...