---
_id: '1061'
abstract:
- lang: eng
  text: 'Background: Metabolic engineering and synthetic biology of cyanobacteria
    offer a promising sustainable alternative approach for fossil-based ethylene production,
    by using sunlight via oxygenic photosynthesis, to convert carbon dioxide directly
    into ethylene. Towards this, both well-studied cyanobacteria, i.e., Synechocystis
    sp PCC 6803 and Synechococcus elongatus PCC 7942, have been engineered to produce
    ethylene by introducing the ethylene-forming enzyme (Efe) from Pseudomonas syringae
    pv. phaseolicola PK2 (the Kudzu strain), which catalyzes the conversion of the
    ubiquitous tricarboxylic acid cycle intermediate 2-oxoglutarate into ethylene.
    Results: This study focuses on Synechocystis sp PCC 6803 and shows stable ethylene
    production through the integration of a codon-optimized version of the efe gene
    under control of the Ptrc promoter and the core Shine-Dalgarno sequence (5\''-AGGAGG-3\'')
    as the ribosome-binding site (RBS), at the slr0168 neutral site. We have increased
    ethylene production twofold by RBS screening and further investigated improving
    ethylene production from a single gene copy of efe, using multiple tandem promoters
    and by putting our best construct on an RSF1010-based broad-host-self-replicating
    plasmid, which has a higher copy number than the genome. Moreover, to raise the
    intracellular amounts of the key Efe substrate, 2-oxoglutarate, from which ethylene
    is formed, we constructed a glycogen-synthesis knockout mutant (glgC) and introduced
    the ethylene biosynthetic pathway in it. Under nitrogen limiting conditions, the
    glycogen knockout strain has increased intracellular 2-oxoglutarate levels; however,
    surprisingly, ethylene production was lower in this strain than in the wild-type
    background. Conclusion: Making use of different RBS sequences, production of ethylene
    ranging over a 20-fold difference has been achieved. However, a further increase
    of production through multiple tandem promoters and a broad-host plasmid was not
    achieved speculating that the transcription strength and the gene copy number
    are not the limiting factors in our system.'
article_number: '34'
article_processing_charge: No
author:
- first_name: Vinod
  full_name: Veetil, Vinod
  last_name: Veetil
- first_name: Andreas
  full_name: Angermayr, Andreas
  id: 4677C796-F248-11E8-B48F-1D18A9856A87
  last_name: Angermayr
  orcid: 0000-0001-8619-2223
- first_name: Klaas
  full_name: Hellingwerf, Klaas
  last_name: Hellingwerf
citation:
  ama: Veetil V, Angermayr A, Hellingwerf K. Ethylene production with engineered Synechocystis
    sp PCC 6803 strains. <i>Microbial Cell Factories</i>. 2017;16(1). doi:<a href="https://doi.org/10.1186/s12934-017-0645-5">10.1186/s12934-017-0645-5</a>
  apa: Veetil, V., Angermayr, A., &#38; Hellingwerf, K. (2017). Ethylene production
    with engineered Synechocystis sp PCC 6803 strains. <i>Microbial Cell Factories</i>.
    BioMed Central. <a href="https://doi.org/10.1186/s12934-017-0645-5">https://doi.org/10.1186/s12934-017-0645-5</a>
  chicago: Veetil, Vinod, Andreas Angermayr, and Klaas Hellingwerf. “Ethylene Production
    with Engineered Synechocystis Sp PCC 6803 Strains.” <i>Microbial Cell Factories</i>.
    BioMed Central, 2017. <a href="https://doi.org/10.1186/s12934-017-0645-5">https://doi.org/10.1186/s12934-017-0645-5</a>.
  ieee: V. Veetil, A. Angermayr, and K. Hellingwerf, “Ethylene production with engineered
    Synechocystis sp PCC 6803 strains,” <i>Microbial Cell Factories</i>, vol. 16,
    no. 1. BioMed Central, 2017.
  ista: Veetil V, Angermayr A, Hellingwerf K. 2017. Ethylene production with engineered
    Synechocystis sp PCC 6803 strains. Microbial Cell Factories. 16(1), 34.
  mla: Veetil, Vinod, et al. “Ethylene Production with Engineered Synechocystis Sp
    PCC 6803 Strains.” <i>Microbial Cell Factories</i>, vol. 16, no. 1, 34, BioMed
    Central, 2017, doi:<a href="https://doi.org/10.1186/s12934-017-0645-5">10.1186/s12934-017-0645-5</a>.
  short: V. Veetil, A. Angermayr, K. Hellingwerf, Microbial Cell Factories 16 (2017).
date_created: 2018-12-11T11:49:56Z
date_published: 2017-02-23T00:00:00Z
date_updated: 2023-09-20T12:09:21Z
day: '23'
ddc:
- '579'
doi: 10.1186/s12934-017-0645-5
extern: '1'
external_id:
  isi:
  - '000397733000001'
  pmid:
  - '28231787'
file:
- access_level: open_access
  content_type: application/pdf
  creator: system
  date_created: 2018-12-12T10:16:50Z
  date_updated: 2018-12-12T10:16:50Z
  file_id: '5240'
  file_name: IST-2017-792-v1+1_s12934-017-0645-5.pdf
  file_size: 1361313
  relation: main_file
file_date_updated: 2018-12-12T10:16:50Z
has_accepted_license: '1'
intvolume: '        16'
isi: 1
issue: '1'
language:
- iso: eng
month: '02'
oa: 1
oa_version: Published Version
pmid: 1
publication: Microbial Cell Factories
publication_identifier:
  issn:
  - '14752859'
publication_status: published
publisher: BioMed Central
publist_id: '6325'
pubrep_id: '792'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Ethylene production with engineered Synechocystis sp PCC 6803 strains
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 16
year: '2017'
...