---
_id: '1006'
abstract:
- lang: eng
  text: 'Background: The phenomenon of immune priming, i.e. enhanced protection following
    a secondary exposure to a pathogen, has now been demonstrated in a wide range
    of invertebrate species. Despite accumulating phenotypic evidence, knowledge of
    its mechanistic underpinnings is currently very limited. Here we used the system
    of the red flour beetle, Tribolium castaneum and the insect pathogen Bacillus
    thuringiensis (Bt) to further our molecular understanding of the oral immune priming
    phenomenon. We addressed how ingestion of bacterial cues (derived from spore supernatants)
    of an orally pathogenic and non-pathogenic Bt strain affects gene expression upon
    later challenge exposure, using a whole-transcriptome sequencing approach. Results:
    Whereas gene expression of individuals primed with the orally non-pathogenic strain
    showed minor changes to controls, we found that priming with the pathogenic strain
    induced regulation of a large set of distinct genes, many of which are known immune
    candidates. Intriguingly, the immune repertoire activated upon priming and subsequent
    challenge qualitatively differed from the one mounted upon infection with Bt without
    previous priming. Moreover, a large subset of priming-specific genes showed an
    inverse regulation compared to their regulation upon challenge only. Conclusions:
    Our data demonstrate that gene expression upon infection is strongly affected
    by previous immune priming. We hypothesise that this shift in gene expression
    indicates activation of a more targeted and efficient response towards a previously
    encountered pathogen, in anticipation of potential secondary encounter.'
article_processing_charge: No
author:
- first_name: Jenny
  full_name: Greenwood, Jenny
  last_name: Greenwood
- first_name: Barbara
  full_name: Milutinovic, Barbara
  id: 2CDC32B8-F248-11E8-B48F-1D18A9856A87
  last_name: Milutinovic
  orcid: 0000-0002-8214-4758
- first_name: Robert
  full_name: Peuß, Robert
  last_name: Peuß
- first_name: Sarah
  full_name: Behrens, Sarah
  last_name: Behrens
- first_name: Daniela
  full_name: Essar, Daniela
  last_name: Essar
- first_name: Philip
  full_name: Rosenstiel, Philip
  last_name: Rosenstiel
- first_name: Hinrich
  full_name: Schulenburg, Hinrich
  last_name: Schulenburg
- first_name: Joachim
  full_name: Kurtz, Joachim
  last_name: Kurtz
citation:
  ama: Greenwood J, Milutinovic B, Peuß R, et al. Oral immune priming with Bacillus
    thuringiensis induces a shift in the gene expression of Tribolium castaneum larvae.
    <i>BMC Genomics</i>. 2017;18(1):329. doi:<a href="https://doi.org/10.1186/s12864-017-3705-7">10.1186/s12864-017-3705-7</a>
  apa: Greenwood, J., Milutinovic, B., Peuß, R., Behrens, S., Essar, D., Rosenstiel,
    P., … Kurtz, J. (2017). Oral immune priming with Bacillus thuringiensis induces
    a shift in the gene expression of Tribolium castaneum larvae. <i>BMC Genomics</i>.
    BioMed Central. <a href="https://doi.org/10.1186/s12864-017-3705-7">https://doi.org/10.1186/s12864-017-3705-7</a>
  chicago: Greenwood, Jenny, Barbara Milutinovic, Robert Peuß, Sarah Behrens, Daniela
    Essar, Philip Rosenstiel, Hinrich Schulenburg, and Joachim Kurtz. “Oral Immune
    Priming with Bacillus Thuringiensis Induces a Shift in the Gene Expression of
    Tribolium Castaneum Larvae.” <i>BMC Genomics</i>. BioMed Central, 2017. <a href="https://doi.org/10.1186/s12864-017-3705-7">https://doi.org/10.1186/s12864-017-3705-7</a>.
  ieee: J. Greenwood <i>et al.</i>, “Oral immune priming with Bacillus thuringiensis
    induces a shift in the gene expression of Tribolium castaneum larvae,” <i>BMC
    Genomics</i>, vol. 18, no. 1. BioMed Central, p. 329, 2017.
  ista: Greenwood J, Milutinovic B, Peuß R, Behrens S, Essar D, Rosenstiel P, Schulenburg
    H, Kurtz J. 2017. Oral immune priming with Bacillus thuringiensis induces a shift
    in the gene expression of Tribolium castaneum larvae. BMC Genomics. 18(1), 329.
  mla: Greenwood, Jenny, et al. “Oral Immune Priming with Bacillus Thuringiensis Induces
    a Shift in the Gene Expression of Tribolium Castaneum Larvae.” <i>BMC Genomics</i>,
    vol. 18, no. 1, BioMed Central, 2017, p. 329, doi:<a href="https://doi.org/10.1186/s12864-017-3705-7">10.1186/s12864-017-3705-7</a>.
  short: J. Greenwood, B. Milutinovic, R. Peuß, S. Behrens, D. Essar, P. Rosenstiel,
    H. Schulenburg, J. Kurtz, BMC Genomics 18 (2017) 329.
date_created: 2018-12-11T11:49:39Z
date_published: 2017-04-26T00:00:00Z
date_updated: 2023-09-22T09:47:44Z
day: '26'
ddc:
- '570'
department:
- _id: SyCr
doi: 10.1186/s12864-017-3705-7
external_id:
  isi:
  - '000400625200004'
file:
- access_level: open_access
  content_type: application/pdf
  creator: system
  date_created: 2018-12-12T10:16:46Z
  date_updated: 2018-12-12T10:16:46Z
  file_id: '5236'
  file_name: IST-2017-814-v1+1_s12864-017-3705-7.pdf
  file_size: 2379672
  relation: main_file
file_date_updated: 2018-12-12T10:16:46Z
has_accepted_license: '1'
intvolume: '        18'
isi: 1
issue: '1'
language:
- iso: eng
month: '04'
oa: 1
oa_version: Published Version
page: '329'
publication: BMC Genomics
publication_identifier:
  issn:
  - '14712164'
publication_status: published
publisher: BioMed Central
publist_id: '6392'
pubrep_id: '814'
quality_controlled: '1'
related_material:
  record:
  - id: '9859'
    relation: research_data
    status: public
  - id: '9860'
    relation: research_data
    status: public
scopus_import: '1'
status: public
title: Oral immune priming with Bacillus thuringiensis induces a shift in the gene
  expression of Tribolium castaneum larvae
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 18
year: '2017'
...