---
_id: '12196'
abstract:
- lang: eng
  text: SNC1 (SUPPRESSOR OF NPR1, CONSTITUTIVE 1) is one of a suite of intracellular
    Arabidopsis NOD-like receptor (NLR) proteins which, upon activation, result in
    the induction of defense responses. However, the molecular mechanisms underlying
    NLR activation and the subsequent provocation of immune responses are only partially
    characterized. To identify negative regulators of NLR-mediated immunity, a forward
    genetic screen was undertaken to search for enhancers of the dwarf, autoimmune
    gain-of-function snc1 mutant. To avoid lethality resulting from severe dwarfism,
    the screen was conducted using mos4 (modifier of snc1, 4) snc1 plants, which display
    wild-type-like morphology and resistance. M2 progeny were screened for mutant,
    snc1-enhancing (muse) mutants displaying a reversion to snc1-like phenotypes.
    The muse9 mos4 snc1 triple mutant was found to exhibit dwarf morphology, elevated
    expression of the pPR2-GUS defense marker reporter gene and enhanced resistance
    to the oomycete pathogen Hyaloperonospora arabidopsidis Noco2. Via map-based cloning
    and Illumina sequencing, it was determined that the muse9 mutation is in the gene
    encoding the SWI/SNF chromatin remodeler SYD (SPLAYED), and was thus renamed syd-10.
    The syd-10 single mutant has no observable alteration from wild-type-like resistance,
    although the syd-4 T-DNA insertion allele displays enhanced resistance to the
    bacterial pathogen Pseudomonas syringae pv. maculicola ES4326. Transcription of
    SNC1 is increased in both syd-4 and syd-10. These data suggest that SYD plays
    a subtle, specific role in the regulation of SNC1 expression and SNC1-mediated
    immunity. SYD may work with other proteins at the chromatin level to repress SNC1
    transcription; such regulation is important for fine-tuning the expression of
    NLR-encoding genes to prevent unpropitious autoimmunity.
acknowledgement: "This work was supported by the National Sciences and Engineering
  Research Council of Canada [Canada Graduate\r\nScholarship–Doctoral to K.J.; Discovery
  Grant to X.L.]; the department of Botany at the University of f British Columbia\r\n[the
  Dewar Cooper Memorial Fund to X.L.].The authors would like to thank Dr. Yuelin Zhang
  and Ms. Yan Li for their assistance with next-generation sequencing, and Mr. Charles
  Copeland for critical reading of the manuscript."
article_processing_charge: No
article_type: original
author:
- first_name: Kaeli C.M.
  full_name: Johnson, Kaeli C.M.
  last_name: Johnson
- first_name: Shitou
  full_name: Xia, Shitou
  last_name: Xia
- first_name: Xiaoqi
  full_name: Feng, Xiaoqi
  id: e0164712-22ee-11ed-b12a-d80fcdf35958
  last_name: Feng
  orcid: 0000-0002-4008-1234
- first_name: Xin
  full_name: Li, Xin
  last_name: Li
citation:
  ama: Johnson KCM, Xia S, Feng X, Li X. The chromatin remodeler SPLAYED negatively
    regulates SNC1-mediated immunity. <i>Plant and Cell Physiology</i>. 2015;56(8):1616-1623.
    doi:<a href="https://doi.org/10.1093/pcp/pcv087">10.1093/pcp/pcv087</a>
  apa: Johnson, K. C. M., Xia, S., Feng, X., &#38; Li, X. (2015). The chromatin remodeler
    SPLAYED negatively regulates SNC1-mediated immunity. <i>Plant and Cell Physiology</i>.
    Oxford University Press. <a href="https://doi.org/10.1093/pcp/pcv087">https://doi.org/10.1093/pcp/pcv087</a>
  chicago: Johnson, Kaeli C.M., Shitou Xia, Xiaoqi Feng, and Xin Li. “The Chromatin
    Remodeler SPLAYED Negatively Regulates SNC1-Mediated Immunity.” <i>Plant and Cell
    Physiology</i>. Oxford University Press, 2015. <a href="https://doi.org/10.1093/pcp/pcv087">https://doi.org/10.1093/pcp/pcv087</a>.
  ieee: K. C. M. Johnson, S. Xia, X. Feng, and X. Li, “The chromatin remodeler SPLAYED
    negatively regulates SNC1-mediated immunity,” <i>Plant and Cell Physiology</i>,
    vol. 56, no. 8. Oxford University Press, pp. 1616–1623, 2015.
  ista: Johnson KCM, Xia S, Feng X, Li X. 2015. The chromatin remodeler SPLAYED negatively
    regulates SNC1-mediated immunity. Plant and Cell Physiology. 56(8), 1616–1623.
  mla: Johnson, Kaeli C. M., et al. “The Chromatin Remodeler SPLAYED Negatively Regulates
    SNC1-Mediated Immunity.” <i>Plant and Cell Physiology</i>, vol. 56, no. 8, Oxford
    University Press, 2015, pp. 1616–23, doi:<a href="https://doi.org/10.1093/pcp/pcv087">10.1093/pcp/pcv087</a>.
  short: K.C.M. Johnson, S. Xia, X. Feng, X. Li, Plant and Cell Physiology 56 (2015)
    1616–1623.
date_created: 2023-01-16T09:20:22Z
date_published: 2015-08-01T00:00:00Z
date_updated: 2023-05-08T11:03:23Z
department:
- _id: XiFe
doi: 10.1093/pcp/pcv087
extern: '1'
external_id:
  pmid:
  - '26063389'
intvolume: '        56'
issue: '8'
keyword:
- Cell Biology
- Plant Science
- Physiology
- General Medicine
language:
- iso: eng
month: '08'
oa_version: None
page: 1616-1623
pmid: 1
publication: Plant and Cell Physiology
publication_identifier:
  issn:
  - 0032-0781
  - 1471-9053
publication_status: published
publisher: Oxford University Press
quality_controlled: '1'
scopus_import: '1'
status: public
title: The chromatin remodeler SPLAYED negatively regulates SNC1-mediated immunity
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 56
year: '2015'
...