---
_id: '3439'
abstract:
- lang: eng
  text: High molecular weight DNA was extracted from the primary Neotropical malaria
    vector, Anopheles darlingi from Capanema, Pará, Brazil, to create a small insert
    genomic library, and then a phagemid library. Enriched sublibraries were constructed
    from the phagemid library using a microsatellite oligo primed second strand synthesis
    protocol. The resulting 242 760 individual clones were screened. The mean clone
    size of the positive clones was 302 bp. Flanking primers were designed for each
    suitable microsatellite sequence. Eight polymorphic loci were optimized and characterized.
    The allele size ranges are based on 253 samples of A. darlingi from eastern Amazonian
    and central Brazil.
acknowledgement: For  support  in  Brazil  we  thank  D.  Galiza,  R.N.L.  Lacerda,E.P.
  Santa Rosa, M.N.O. Segura, and R.T.L. de Souza. We also thankM.J.  Braun  for  allowing  work  on  the  library  construction  at  theLaboratory
  of Molecular Systematics, Washington. Supported byNIH AI 40116 to JEC and Instituto
  Evandro Chagas, Belém, Brazil.
article_processing_charge: No
article_type: original
author:
- first_name: Jan
  full_name: Conn, Jan
  last_name: Conn
- first_name: Jonathan P
  full_name: Bollback, Jonathan P
  id: 2C6FA9CC-F248-11E8-B48F-1D18A9856A87
  last_name: Bollback
  orcid: 0000-0002-4624-4612
- first_name: David
  full_name: Onyabe, David
  last_name: Onyabe
- first_name: Tessa
  full_name: Robinson, Tessa
  last_name: Robinson
- first_name: Richard
  full_name: Wilkerson, Richard
  last_name: Wilkerson
- first_name: Marinete
  full_name: Povoa, Marinete
  last_name: Povoa
citation:
  ama: Conn J, Bollback JP, Onyabe D, Robinson T, Wilkerson R, Povoa M. Isolation
    of polymorphic microsatellite markers from the malaria vector Anopheles darlingi.
    <i>Molecular Ecology Notes</i>. 2001;1(4):223-225. doi:<a href="https://doi.org/
    10.1046/j.1471-8278.2001.00078.x"> 10.1046/j.1471-8278.2001.00078.x</a>
  apa: Conn, J., Bollback, J. P., Onyabe, D., Robinson, T., Wilkerson, R., &#38; Povoa,
    M. (2001). Isolation of polymorphic microsatellite markers from the malaria vector
    Anopheles darlingi. <i>Molecular Ecology Notes</i>. Wiley-Blackwell. <a href="https://doi.org/
    10.1046/j.1471-8278.2001.00078.x">https://doi.org/ 10.1046/j.1471-8278.2001.00078.x</a>
  chicago: Conn, Jan, Jonathan P Bollback, David Onyabe, Tessa Robinson, Richard Wilkerson,
    and Marinete Povoa. “Isolation of Polymorphic Microsatellite Markers from the
    Malaria Vector Anopheles Darlingi.” <i>Molecular Ecology Notes</i>. Wiley-Blackwell,
    2001. <a href="https://doi.org/ 10.1046/j.1471-8278.2001.00078.x">https://doi.org/
    10.1046/j.1471-8278.2001.00078.x</a>.
  ieee: J. Conn, J. P. Bollback, D. Onyabe, T. Robinson, R. Wilkerson, and M. Povoa,
    “Isolation of polymorphic microsatellite markers from the malaria vector Anopheles
    darlingi,” <i>Molecular Ecology Notes</i>, vol. 1, no. 4. Wiley-Blackwell, pp.
    223–225, 2001.
  ista: Conn J, Bollback JP, Onyabe D, Robinson T, Wilkerson R, Povoa M. 2001. Isolation
    of polymorphic microsatellite markers from the malaria vector Anopheles darlingi.
    Molecular Ecology Notes. 1(4), 223–225.
  mla: Conn, Jan, et al. “Isolation of Polymorphic Microsatellite Markers from the
    Malaria Vector Anopheles Darlingi.” <i>Molecular Ecology Notes</i>, vol. 1, no.
    4, Wiley-Blackwell, 2001, pp. 223–25, doi:<a href="https://doi.org/ 10.1046/j.1471-8278.2001.00078.x">
    10.1046/j.1471-8278.2001.00078.x</a>.
  short: J. Conn, J.P. Bollback, D. Onyabe, T. Robinson, R. Wilkerson, M. Povoa, Molecular
    Ecology Notes 1 (2001) 223–225.
date_created: 2018-12-11T12:03:20Z
date_published: 2001-12-01T00:00:00Z
date_updated: 2023-05-15T13:58:49Z
day: '01'
doi: ' 10.1046/j.1471-8278.2001.00078.x'
extern: '1'
intvolume: '         1'
issue: '4'
language:
- iso: eng
month: '12'
oa_version: None
page: 223 - 225
publication: Molecular Ecology Notes
publication_identifier:
  issn:
  - 1471-8278
publication_status: published
publisher: Wiley-Blackwell
publist_id: '2961'
quality_controlled: '1'
status: public
title: Isolation of polymorphic microsatellite markers from the malaria vector Anopheles
  darlingi
type: journal_article
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 1
year: '2001'
...