[{"date_created":"2021-07-04T22:01:25Z","month":"06","page":"733–744","status":"public","intvolume":"        23","department":[{"_id":"EdHa"}],"quality_controlled":"1","publication":"Nature Cell Biology","isi":1,"publisher":"Springer Nature","type":"journal_article","author":[{"full_name":"Yang, Qiutan","first_name":"Qiutan","last_name":"Yang"},{"last_name":"Xue","first_name":"Shi-lei","full_name":"Xue, Shi-lei","id":"31D2C804-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Chii Jou","full_name":"Chan, Chii Jou","last_name":"Chan"},{"first_name":"Markus","full_name":"Rempfler, Markus","last_name":"Rempfler"},{"full_name":"Vischi, Dario","first_name":"Dario","last_name":"Vischi"},{"last_name":"Maurer-Gutierrez","full_name":"Maurer-Gutierrez, Francisca","first_name":"Francisca"},{"last_name":"Hiiragi","first_name":"Takashi","full_name":"Hiiragi, Takashi"},{"orcid":"0000-0001-6005-1561","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","full_name":"Hannezo, Edouard B","first_name":"Edouard B","last_name":"Hannezo"},{"first_name":"Prisca","full_name":"Liberali, Prisca","last_name":"Liberali"}],"day":"21","title":"Cell fate coordinates mechano-osmotic forces in intestinal crypt formation","ec_funded":1,"citation":{"mla":"Yang, Qiutan, et al. “Cell Fate Coordinates Mechano-Osmotic Forces in Intestinal Crypt Formation.” <i>Nature Cell Biology</i>, vol. 23, Springer Nature, 2021, pp. 733–744, doi:<a href=\"https://doi.org/10.1038/s41556-021-00700-2\">10.1038/s41556-021-00700-2</a>.","ieee":"Q. Yang <i>et al.</i>, “Cell fate coordinates mechano-osmotic forces in intestinal crypt formation,” <i>Nature Cell Biology</i>, vol. 23. Springer Nature, pp. 733–744, 2021.","ista":"Yang Q, Xue S, Chan CJ, Rempfler M, Vischi D, Maurer-Gutierrez F, Hiiragi T, Hannezo EB, Liberali P. 2021. Cell fate coordinates mechano-osmotic forces in intestinal crypt formation. Nature Cell Biology. 23, 733–744.","chicago":"Yang, Qiutan, Shi-lei Xue, Chii Jou Chan, Markus Rempfler, Dario Vischi, Francisca Maurer-Gutierrez, Takashi Hiiragi, Edouard B Hannezo, and Prisca Liberali. “Cell Fate Coordinates Mechano-Osmotic Forces in Intestinal Crypt Formation.” <i>Nature Cell Biology</i>. Springer Nature, 2021. <a href=\"https://doi.org/10.1038/s41556-021-00700-2\">https://doi.org/10.1038/s41556-021-00700-2</a>.","apa":"Yang, Q., Xue, S., Chan, C. J., Rempfler, M., Vischi, D., Maurer-Gutierrez, F., … Liberali, P. (2021). Cell fate coordinates mechano-osmotic forces in intestinal crypt formation. <i>Nature Cell Biology</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41556-021-00700-2\">https://doi.org/10.1038/s41556-021-00700-2</a>","ama":"Yang Q, Xue S, Chan CJ, et al. Cell fate coordinates mechano-osmotic forces in intestinal crypt formation. <i>Nature Cell Biology</i>. 2021;23:733–744. doi:<a href=\"https://doi.org/10.1038/s41556-021-00700-2\">10.1038/s41556-021-00700-2</a>","short":"Q. Yang, S. Xue, C.J. Chan, M. Rempfler, D. Vischi, F. Maurer-Gutierrez, T. Hiiragi, E.B. Hannezo, P. Liberali, Nature Cell Biology 23 (2021) 733–744."},"doi":"10.1038/s41556-021-00700-2","language":[{"iso":"eng"}],"acknowledgement":"We acknowledge the members of the Lennon-Duménil laboratory for sharing the mouse line of Myh9-GFP. We are grateful to the members of the Liberali laboratory and the FMI facilities for their support. We thank E. Tagliavini for IT support; L. Gelman for assistance and training; S. Bichet and A. Bogucki for helping with histology of mouse tissues; H. Kohler for fluorescence-activated cell sorting; G. Q. G. de Medeiros for maintenance of light-sheet microscopy; M. G. Stadler for scRNA-seq analysis; G. Gay for discussions on the 3D vertex model; the members of the Liberali laboratory, C. P. Heisenberg and C. Tsiairis for reading and providing feedback on the manuscript. Funding: Q.Y. is supported by a Postdoc fellowship from Peter und Taul Engelhorn Stiftung (PTES). This work received funding from the European Research Council (ERC) under the EU Horizon 2020 research and Innovation Programme Grant Agreement no. 758617 (to P.L.), the Swiss National Foundation (SNF) (POOP3_157531, to P.L.) and from the ERC under the EU Horizon 2020 Research and Innovation Program Grant Agreements 851288 (to E.H.) and the Austrian Science Fund (FWF) (P31639, to E.H.).","pmid":1,"project":[{"grant_number":"851288","name":"Design Principles of Branching Morphogenesis","_id":"05943252-7A3F-11EA-A408-12923DDC885E","call_identifier":"H2020"},{"call_identifier":"FWF","_id":"268294B6-B435-11E9-9278-68D0E5697425","name":"Active mechano-chemical description of the cell cytoskeleton","grant_number":"P31639"}],"date_published":"2021-06-21T00:00:00Z","_id":"9629","abstract":[{"text":"Intestinal organoids derived from single cells undergo complex crypt–villus patterning and morphogenesis. However, the nature and coordination of the underlying forces remains poorly characterized. Here, using light-sheet microscopy and large-scale imaging quantification, we demonstrate that crypt formation coincides with a stark reduction in lumen volume. We develop a 3D biophysical model to computationally screen different mechanical scenarios of crypt morphogenesis. Combining this with live-imaging data and multiple mechanical perturbations, we show that actomyosin-driven crypt apical contraction and villus basal tension work synergistically with lumen volume reduction to drive crypt morphogenesis, and demonstrate the existence of a critical point in differential tensions above which crypt morphology becomes robust to volume changes. Finally, we identified a sodium/glucose cotransporter that is specific to differentiated enterocytes that modulates lumen volume reduction through cell swelling in the villus region. Together, our study uncovers the cellular basis of how cell fate modulates osmotic and actomyosin forces to coordinate robust morphogenesis.","lang":"eng"}],"article_processing_charge":"No","volume":23,"oa":1,"main_file_link":[{"open_access":"1","url":"https://www.biorxiv.org/content/10.1101/2020.05.13.094359"}],"publication_status":"published","year":"2021","oa_version":"Preprint","article_type":"original","publication_identifier":{"eissn":["1476-4679"],"issn":["1465-7392"]},"date_updated":"2023-08-10T13:57:36Z","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","scopus_import":"1","external_id":{"isi":["000664016300003"],"pmid":["34155381"]}},{"volume":21,"publication_status":"published","oa":1,"main_file_link":[{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7025891","open_access":"1"}],"_id":"7105","abstract":[{"text":"Cell migration is hypothesized to involve a cycle of behaviours beginning with leading edge extension. However, recent evidence suggests that the leading edge may be dispensable for migration, raising the question of what actually controls cell directionality. Here, we exploit the embryonic migration of Drosophila macrophages to bridge the different temporal scales of the behaviours controlling motility. This approach reveals that edge fluctuations during random motility are not persistent and are weakly correlated with motion. In contrast, flow of the actin network behind the leading edge is highly persistent. Quantification of actin flow structure during migration reveals a stable organization and asymmetry in the cell-wide flowfield that strongly correlates with cell directionality. This organization is regulated by a gradient of actin network compression and destruction, which is controlled by myosin contraction and cofilin-mediated disassembly. It is this stable actin-flow polarity, which integrates rapid fluctuations of the leading edge, that controls inherent cellular persistence.","lang":"eng"}],"date_published":"2019-11-01T00:00:00Z","issue":"11","article_processing_charge":"No","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","date_updated":"2023-09-06T11:08:52Z","scopus_import":"1","external_id":{"isi":["000495888300009"],"pmid":["31685997"]},"publication_identifier":{"eissn":["1476-4679"],"issn":["1465-7392"]},"article_type":"original","year":"2019","oa_version":"Submitted Version","department":[{"_id":"MiSi"}],"quality_controlled":"1","publication":"Nature Cell Biology","intvolume":"        21","status":"public","publisher":"Springer Nature","isi":1,"month":"11","date_created":"2019-11-25T08:55:00Z","page":"1370-1381","language":[{"iso":"eng"}],"doi":"10.1038/s41556-019-0411-5","pmid":1,"day":"01","author":[{"full_name":"Yolland, Lawrence","first_name":"Lawrence","last_name":"Yolland"},{"last_name":"Burki","full_name":"Burki, Mubarik","first_name":"Mubarik"},{"last_name":"Marcotti","first_name":"Stefania","full_name":"Marcotti, Stefania"},{"last_name":"Luchici","full_name":"Luchici, Andrei","first_name":"Andrei"},{"last_name":"Kenny","full_name":"Kenny, Fiona N.","first_name":"Fiona N."},{"last_name":"Davis","first_name":"John Robert","full_name":"Davis, John Robert"},{"last_name":"Serna-Morales","full_name":"Serna-Morales, Eduardo","first_name":"Eduardo"},{"last_name":"Müller","first_name":"Jan","full_name":"Müller, Jan","id":"AD07FDB4-0F61-11EA-8158-C4CC64CEAA8D"},{"first_name":"Michael K","full_name":"Sixt, Michael K","last_name":"Sixt","orcid":"0000-0002-6620-9179","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Davidson","full_name":"Davidson, Andrew","first_name":"Andrew"},{"full_name":"Wood, Will","first_name":"Will","last_name":"Wood"},{"last_name":"Schumacher","full_name":"Schumacher, Linus J.","first_name":"Linus J."},{"last_name":"Endres","full_name":"Endres, Robert G.","first_name":"Robert G."},{"full_name":"Miodownik, Mark","first_name":"Mark","last_name":"Miodownik"},{"last_name":"Stramer","first_name":"Brian M.","full_name":"Stramer, Brian M."}],"type":"journal_article","citation":{"mla":"Yolland, Lawrence, et al. “Persistent and Polarized Global Actin Flow Is Essential for Directionality during Cell Migration.” <i>Nature Cell Biology</i>, vol. 21, no. 11, Springer Nature, 2019, pp. 1370–81, doi:<a href=\"https://doi.org/10.1038/s41556-019-0411-5\">10.1038/s41556-019-0411-5</a>.","chicago":"Yolland, Lawrence, Mubarik Burki, Stefania Marcotti, Andrei Luchici, Fiona N. Kenny, John Robert Davis, Eduardo Serna-Morales, et al. “Persistent and Polarized Global Actin Flow Is Essential for Directionality during Cell Migration.” <i>Nature Cell Biology</i>. Springer Nature, 2019. <a href=\"https://doi.org/10.1038/s41556-019-0411-5\">https://doi.org/10.1038/s41556-019-0411-5</a>.","ista":"Yolland L, Burki M, Marcotti S, Luchici A, Kenny FN, Davis JR, Serna-Morales E, Müller J, Sixt MK, Davidson A, Wood W, Schumacher LJ, Endres RG, Miodownik M, Stramer BM. 2019. Persistent and polarized global actin flow is essential for directionality during cell migration. Nature Cell Biology. 21(11), 1370–1381.","ieee":"L. Yolland <i>et al.</i>, “Persistent and polarized global actin flow is essential for directionality during cell migration,” <i>Nature Cell Biology</i>, vol. 21, no. 11. Springer Nature, pp. 1370–1381, 2019.","ama":"Yolland L, Burki M, Marcotti S, et al. Persistent and polarized global actin flow is essential for directionality during cell migration. <i>Nature Cell Biology</i>. 2019;21(11):1370-1381. doi:<a href=\"https://doi.org/10.1038/s41556-019-0411-5\">10.1038/s41556-019-0411-5</a>","apa":"Yolland, L., Burki, M., Marcotti, S., Luchici, A., Kenny, F. N., Davis, J. R., … Stramer, B. M. (2019). Persistent and polarized global actin flow is essential for directionality during cell migration. <i>Nature Cell Biology</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41556-019-0411-5\">https://doi.org/10.1038/s41556-019-0411-5</a>","short":"L. Yolland, M. Burki, S. Marcotti, A. Luchici, F.N. Kenny, J.R. Davis, E. Serna-Morales, J. Müller, M.K. Sixt, A. Davidson, W. Wood, L.J. Schumacher, R.G. Endres, M. Miodownik, B.M. Stramer, Nature Cell Biology 21 (2019) 1370–1381."},"title":"Persistent and polarized global actin flow is essential for directionality during cell migration"},{"publication":"Nature Cell Biology","quality_controlled":"1","status":"public","intvolume":"        21","publisher":"Springer Nature","month":"08","extern":"1","date_created":"2020-02-11T08:43:49Z","page":"924-932","language":[{"iso":"eng"}],"doi":"10.1038/s41556-019-0362-x","pmid":1,"day":"01","type":"journal_article","author":[{"last_name":"Andersen","full_name":"Andersen, Marianne Stemann","first_name":"Marianne Stemann"},{"last_name":"Hannezo","first_name":"Edouard B","full_name":"Hannezo, Edouard B","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-6005-1561"},{"last_name":"Ulyanchenko","full_name":"Ulyanchenko, Svetlana","first_name":"Svetlana"},{"last_name":"Estrach","first_name":"Soline","full_name":"Estrach, Soline"},{"last_name":"Antoku","full_name":"Antoku, Yasuko","first_name":"Yasuko"},{"last_name":"Pisano","full_name":"Pisano, Sabrina","first_name":"Sabrina"},{"last_name":"Boonekamp","first_name":"Kim E.","full_name":"Boonekamp, Kim E."},{"full_name":"Sendrup, Sarah","first_name":"Sarah","last_name":"Sendrup"},{"last_name":"Maimets","first_name":"Martti","full_name":"Maimets, Martti"},{"first_name":"Marianne Terndrup","full_name":"Pedersen, Marianne Terndrup","last_name":"Pedersen"},{"last_name":"Johansen","full_name":"Johansen, Jens V.","first_name":"Jens V."},{"full_name":"Clement, Ditte L.","first_name":"Ditte L.","last_name":"Clement"},{"full_name":"Feral, Chloe C.","first_name":"Chloe C.","last_name":"Feral"},{"first_name":"Benjamin D.","full_name":"Simons, Benjamin D.","last_name":"Simons"},{"first_name":"Kim B.","full_name":"Jensen, Kim B.","last_name":"Jensen"}],"citation":{"apa":"Andersen, M. S., Hannezo, E. B., Ulyanchenko, S., Estrach, S., Antoku, Y., Pisano, S., … Jensen, K. B. (2019). Tracing the cellular dynamics of sebaceous gland development in normal and perturbed states. <i>Nature Cell Biology</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41556-019-0362-x\">https://doi.org/10.1038/s41556-019-0362-x</a>","ama":"Andersen MS, Hannezo EB, Ulyanchenko S, et al. Tracing the cellular dynamics of sebaceous gland development in normal and perturbed states. <i>Nature Cell Biology</i>. 2019;21(8):924-932. doi:<a href=\"https://doi.org/10.1038/s41556-019-0362-x\">10.1038/s41556-019-0362-x</a>","short":"M.S. Andersen, E.B. Hannezo, S. Ulyanchenko, S. Estrach, Y. Antoku, S. Pisano, K.E. Boonekamp, S. Sendrup, M. Maimets, M.T. Pedersen, J.V. Johansen, D.L. Clement, C.C. Feral, B.D. Simons, K.B. Jensen, Nature Cell Biology 21 (2019) 924–932.","mla":"Andersen, Marianne Stemann, et al. “Tracing the Cellular Dynamics of Sebaceous Gland Development in Normal and Perturbed States.” <i>Nature Cell Biology</i>, vol. 21, no. 8, Springer Nature, 2019, pp. 924–32, doi:<a href=\"https://doi.org/10.1038/s41556-019-0362-x\">10.1038/s41556-019-0362-x</a>.","ista":"Andersen MS, Hannezo EB, Ulyanchenko S, Estrach S, Antoku Y, Pisano S, Boonekamp KE, Sendrup S, Maimets M, Pedersen MT, Johansen JV, Clement DL, Feral CC, Simons BD, Jensen KB. 2019. Tracing the cellular dynamics of sebaceous gland development in normal and perturbed states. Nature Cell Biology. 21(8), 924–932.","ieee":"M. S. Andersen <i>et al.</i>, “Tracing the cellular dynamics of sebaceous gland development in normal and perturbed states,” <i>Nature Cell Biology</i>, vol. 21, no. 8. Springer Nature, pp. 924–932, 2019.","chicago":"Andersen, Marianne Stemann, Edouard B Hannezo, Svetlana Ulyanchenko, Soline Estrach, Yasuko Antoku, Sabrina Pisano, Kim E. Boonekamp, et al. “Tracing the Cellular Dynamics of Sebaceous Gland Development in Normal and Perturbed States.” <i>Nature Cell Biology</i>. Springer Nature, 2019. <a href=\"https://doi.org/10.1038/s41556-019-0362-x\">https://doi.org/10.1038/s41556-019-0362-x</a>."},"title":"Tracing the cellular dynamics of sebaceous gland development in normal and perturbed states","volume":21,"publication_status":"published","oa":1,"main_file_link":[{"open_access":"1","url":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6978139/"}],"_id":"7476","date_published":"2019-08-01T00:00:00Z","abstract":[{"text":"The sebaceous gland (SG) is an essential component of the skin, and SG dysfunction is debilitating1,2. Yet, the cellular bases for its origin, development and subsequent maintenance remain poorly understood. Here, we apply large-scale quantitative fate mapping to define the patterns of cell fate behaviour during SG development and maintenance. We show that the SG develops from a defined number of lineage-restricted progenitors that undergo a programme of independent and stochastic cell fate decisions. Following an expansion phase, equipotent progenitors transition into a phase of homeostatic turnover, which is correlated with changes in the mechanical properties of the stroma and spatial restrictions on gland size. Expression of the oncogene KrasG12D results in a release from these constraints and unbridled gland expansion. Quantitative clonal fate analysis reveals that, during this phase, the primary effect of the Kras oncogene is to drive a constant fate bias with little effect on cell division rates. These findings provide insight into the developmental programme of the SG, as well as the mechanisms that drive tumour progression and gland dysfunction.","lang":"eng"}],"article_processing_charge":"No","issue":"8","external_id":{"pmid":["31358966"]},"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","date_updated":"2021-01-12T08:13:47Z","publication_identifier":{"issn":["1465-7392","1476-4679"]},"article_type":"original","year":"2019","oa_version":"Submitted Version"},{"publisher":"Springer Nature","status":"public","intvolume":"         9","quality_controlled":"1","publication":"Nature Cell Biology","page":"1160-1166","date_created":"2022-04-07T07:56:04Z","extern":"1","month":"09","pmid":1,"doi":"10.1038/ncb1636","language":[{"iso":"eng"}],"keyword":["Cell Biology"],"title":"Nuclear envelope formation by chromatin-mediated reorganization of the endoplasmic reticulum","citation":{"short":"D.J. Anderson, M. Hetzer, Nature Cell Biology 9 (2007) 1160–1166.","apa":"Anderson, D. J., &#38; Hetzer, M. (2007). Nuclear envelope formation by chromatin-mediated reorganization of the endoplasmic reticulum. <i>Nature Cell Biology</i>. Springer Nature. <a href=\"https://doi.org/10.1038/ncb1636\">https://doi.org/10.1038/ncb1636</a>","ama":"Anderson DJ, Hetzer M. Nuclear envelope formation by chromatin-mediated reorganization of the endoplasmic reticulum. <i>Nature Cell Biology</i>. 2007;9(10):1160-1166. doi:<a href=\"https://doi.org/10.1038/ncb1636\">10.1038/ncb1636</a>","ieee":"D. J. Anderson and M. Hetzer, “Nuclear envelope formation by chromatin-mediated reorganization of the endoplasmic reticulum,” <i>Nature Cell Biology</i>, vol. 9, no. 10. Springer Nature, pp. 1160–1166, 2007.","ista":"Anderson DJ, Hetzer M. 2007. Nuclear envelope formation by chromatin-mediated reorganization of the endoplasmic reticulum. Nature Cell Biology. 9(10), 1160–1166.","chicago":"Anderson, Daniel J., and Martin Hetzer. “Nuclear Envelope Formation by Chromatin-Mediated Reorganization of the Endoplasmic Reticulum.” <i>Nature Cell Biology</i>. Springer Nature, 2007. <a href=\"https://doi.org/10.1038/ncb1636\">https://doi.org/10.1038/ncb1636</a>.","mla":"Anderson, Daniel J., and Martin Hetzer. “Nuclear Envelope Formation by Chromatin-Mediated Reorganization of the Endoplasmic Reticulum.” <i>Nature Cell Biology</i>, vol. 9, no. 10, Springer Nature, 2007, pp. 1160–66, doi:<a href=\"https://doi.org/10.1038/ncb1636\">10.1038/ncb1636</a>."},"author":[{"last_name":"Anderson","first_name":"Daniel J.","full_name":"Anderson, Daniel J."},{"last_name":"HETZER","first_name":"Martin W","full_name":"HETZER, Martin W","id":"86c0d31b-b4eb-11ec-ac5a-eae7b2e135ed","orcid":"0000-0002-2111-992X"}],"type":"journal_article","day":"09","publication_status":"published","volume":9,"issue":"10","article_processing_charge":"No","_id":"11115","date_published":"2007-09-09T00:00:00Z","abstract":[{"lang":"eng","text":"The formation of the nuclear envelope (NE) around chromatin is a major membrane-remodelling event that occurs during cell division of metazoa. It is unclear whether the nuclear membrane reforms by the fusion of NE fragments or if it re-emerges from an intact tubular network of the endoplasmic reticulum (ER). Here, we show that NE formation and expansion requires a tubular ER network and occurs efficiently in the presence of the membrane fusion inhibitor GTPγS. Chromatin recruitment of membranes, which is initiated by tubule-end binding, followed by the formation, expansion and sealing of flat membrane sheets, is mediated by DNA-binding proteins residing in the ER. Thus, chromatin plays an active role in reshaping of the ER during NE formation."}],"publication_identifier":{"issn":["1465-7392"],"eissn":["1476-4679"]},"date_updated":"2022-07-18T08:56:38Z","user_id":"72615eeb-f1f3-11ec-aa25-d4573ddc34fd","scopus_import":"1","external_id":{"pmid":["17828249"]},"year":"2007","oa_version":"None","article_type":"original"},{"year":"2002","oa_version":"None","article_type":"original","publication_identifier":{"eissn":["1476-4679"],"issn":["1465-7392"]},"external_id":{"pmid":["12105431"]},"scopus_import":"1","user_id":"72615eeb-f1f3-11ec-aa25-d4573ddc34fd","date_updated":"2022-07-18T08:58:03Z","article_processing_charge":"No","issue":"7","date_published":"2002-07-01T00:00:00Z","_id":"11123","abstract":[{"lang":"eng","text":"The small GTPase Ran is a key regulator of nucleocytoplasmic transport during interphase. The asymmetric distribution of the GTP-bound form of Ran across the nuclear envelope — that is, large quantities in the nucleus compared with small quantities in the cytoplasm — determines the directionality of many nuclear transport processes. Recent findings that Ran also functions in spindle formation and nuclear envelope assembly during mitosis suggest that Ran has a general role in chromatin-centred processes. Ran functions in these events as a signal for chromosome position."}],"publication_status":"published","volume":4,"title":"The Ran GTPase as a marker of chromosome position in spindle formation and nuclear envelope assembly","citation":{"apa":"Hetzer, M., Gruss, O. J., &#38; Mattaj, I. W. (2002). The Ran GTPase as a marker of chromosome position in spindle formation and nuclear envelope assembly. <i>Nature Cell Biology</i>. Springer Nature. <a href=\"https://doi.org/10.1038/ncb0702-e177\">https://doi.org/10.1038/ncb0702-e177</a>","ama":"Hetzer M, Gruss OJ, Mattaj IW. The Ran GTPase as a marker of chromosome position in spindle formation and nuclear envelope assembly. <i>Nature Cell Biology</i>. 2002;4(7):E177-E184. doi:<a href=\"https://doi.org/10.1038/ncb0702-e177\">10.1038/ncb0702-e177</a>","short":"M. Hetzer, O.J. Gruss, I.W. Mattaj, Nature Cell Biology 4 (2002) E177–E184.","mla":"Hetzer, Martin, et al. “The Ran GTPase as a Marker of Chromosome Position in Spindle Formation and Nuclear Envelope Assembly.” <i>Nature Cell Biology</i>, vol. 4, no. 7, Springer Nature, 2002, pp. E177–84, doi:<a href=\"https://doi.org/10.1038/ncb0702-e177\">10.1038/ncb0702-e177</a>.","ista":"Hetzer M, Gruss OJ, Mattaj IW. 2002. The Ran GTPase as a marker of chromosome position in spindle formation and nuclear envelope assembly. Nature Cell Biology. 4(7), E177–E184.","ieee":"M. Hetzer, O. J. Gruss, and I. W. Mattaj, “The Ran GTPase as a marker of chromosome position in spindle formation and nuclear envelope assembly,” <i>Nature Cell Biology</i>, vol. 4, no. 7. Springer Nature, pp. E177–E184, 2002.","chicago":"Hetzer, Martin, Oliver J. Gruss, and Iain W. Mattaj. “The Ran GTPase as a Marker of Chromosome Position in Spindle Formation and Nuclear Envelope Assembly.” <i>Nature Cell Biology</i>. Springer Nature, 2002. <a href=\"https://doi.org/10.1038/ncb0702-e177\">https://doi.org/10.1038/ncb0702-e177</a>."},"author":[{"full_name":"HETZER, Martin W","first_name":"Martin W","last_name":"HETZER","id":"86c0d31b-b4eb-11ec-ac5a-eae7b2e135ed","orcid":"0000-0002-2111-992X"},{"full_name":"Gruss, Oliver J.","first_name":"Oliver J.","last_name":"Gruss"},{"last_name":"Mattaj","full_name":"Mattaj, Iain W.","first_name":"Iain W."}],"type":"journal_article","day":"01","pmid":1,"doi":"10.1038/ncb0702-e177","keyword":["Cell Biology"],"language":[{"iso":"eng"}],"page":"E177-E184","date_created":"2022-04-07T07:57:19Z","month":"07","extern":"1","publisher":"Springer Nature","intvolume":"         4","status":"public","publication":"Nature Cell Biology","quality_controlled":"1"},{"article_type":"original","oa_version":"None","year":"2001","date_updated":"2022-07-18T08:58:07Z","user_id":"72615eeb-f1f3-11ec-aa25-d4573ddc34fd","external_id":{"pmid":["11781570"]},"scopus_import":"1","publication_identifier":{"issn":["1465-7392"],"eissn":["1476-4679"]},"_id":"11125","abstract":[{"lang":"eng","text":"Although nuclear envelope (NE) assembly is known to require the GTPase Ran, the membrane fusion machinery involved is uncharacterized. NE assembly involves formation of a reticular network on chromatin, fusion of this network into a closed NE and subsequent expansion. Here we show that p97, an AAA-ATPase previously implicated in fusion of Golgi and transitional endoplasmic reticulum (ER) membranes together with the adaptor p47, has two discrete functions in NE assembly. Formation of a closed NE requires the p97–Ufd1–Npl4 complex, not previously implicated in membrane fusion. Subsequent NE growth involves a p97–p47 complex. This study provides the first insights into the molecular mechanisms and specificity of fusion events involved in NE formation."}],"date_published":"2001-11-02T00:00:00Z","issue":"12","article_processing_charge":"No","volume":3,"publication_status":"published","day":"02","author":[{"id":"86c0d31b-b4eb-11ec-ac5a-eae7b2e135ed","orcid":"0000-0002-2111-992X","full_name":"HETZER, Martin W","first_name":"Martin W","last_name":"HETZER"},{"last_name":"Meyer","full_name":"Meyer, Hemmo H.","first_name":"Hemmo H."},{"last_name":"Walther","first_name":"Tobias C.","full_name":"Walther, Tobias C."},{"last_name":"Bilbao-Cortes","full_name":"Bilbao-Cortes, Daniel","first_name":"Daniel"},{"full_name":"Warren, Graham","first_name":"Graham","last_name":"Warren"},{"last_name":"Mattaj","full_name":"Mattaj, Iain W.","first_name":"Iain W."}],"type":"journal_article","citation":{"ama":"Hetzer M, Meyer HH, Walther TC, Bilbao-Cortes D, Warren G, Mattaj IW. Distinct AAA-ATPase p97 complexes function in discrete steps of nuclear assembly. <i>Nature Cell Biology</i>. 2001;3(12):1086-1091. doi:<a href=\"https://doi.org/10.1038/ncb1201-1086\">10.1038/ncb1201-1086</a>","apa":"Hetzer, M., Meyer, H. H., Walther, T. C., Bilbao-Cortes, D., Warren, G., &#38; Mattaj, I. W. (2001). Distinct AAA-ATPase p97 complexes function in discrete steps of nuclear assembly. <i>Nature Cell Biology</i>. Springer Nature. <a href=\"https://doi.org/10.1038/ncb1201-1086\">https://doi.org/10.1038/ncb1201-1086</a>","short":"M. Hetzer, H.H. Meyer, T.C. Walther, D. Bilbao-Cortes, G. Warren, I.W. Mattaj, Nature Cell Biology 3 (2001) 1086–1091.","mla":"Hetzer, Martin, et al. “Distinct AAA-ATPase P97 Complexes Function in Discrete Steps of Nuclear Assembly.” <i>Nature Cell Biology</i>, vol. 3, no. 12, Springer Nature, 2001, pp. 1086–91, doi:<a href=\"https://doi.org/10.1038/ncb1201-1086\">10.1038/ncb1201-1086</a>.","chicago":"Hetzer, Martin, Hemmo H. Meyer, Tobias C. Walther, Daniel Bilbao-Cortes, Graham Warren, and Iain W. Mattaj. “Distinct AAA-ATPase P97 Complexes Function in Discrete Steps of Nuclear Assembly.” <i>Nature Cell Biology</i>. Springer Nature, 2001. <a href=\"https://doi.org/10.1038/ncb1201-1086\">https://doi.org/10.1038/ncb1201-1086</a>.","ieee":"M. Hetzer, H. H. Meyer, T. C. Walther, D. Bilbao-Cortes, G. Warren, and I. W. Mattaj, “Distinct AAA-ATPase p97 complexes function in discrete steps of nuclear assembly,” <i>Nature Cell Biology</i>, vol. 3, no. 12. Springer Nature, pp. 1086–1091, 2001.","ista":"Hetzer M, Meyer HH, Walther TC, Bilbao-Cortes D, Warren G, Mattaj IW. 2001. Distinct AAA-ATPase p97 complexes function in discrete steps of nuclear assembly. Nature Cell Biology. 3(12), 1086–1091."},"title":"Distinct AAA-ATPase p97 complexes function in discrete steps of nuclear assembly","language":[{"iso":"eng"}],"keyword":["Cell Biology"],"doi":"10.1038/ncb1201-1086","pmid":1,"extern":"1","month":"11","date_created":"2022-04-07T07:57:42Z","page":"1086-1091","quality_controlled":"1","publication":"Nature Cell Biology","status":"public","intvolume":"         3","publisher":"Springer Nature"}]